Preface Nutraceutical and functional foods has gained immense popularity in the whole world to combat with many diseases. In recent days, people from India and other developing countries spends more time and effort to maintain health through exploring various herbal medicines and natural health care products. The World Health Organization on March 11, 2020, has declared the novel coronavirus (COVID-19) outbreak a global pandemic. Since then, the Severe Acute Respiratory Syndrome Coronavirus 2(SARS-CoV-2) has caused, and is continuing to cause, global disruption, a significant loss of life and extensive human suffering. Studies reveal that nutraceutical and functional foods which include vitamin C, vitamin D, omega-3-polyunsaturated fatty acids, probiotics, and zinc can largely be attributed towards preventive healthcare with the possibility of inhibiting the replication of SARS-CoV-2 and its prognosis. With the ongoing situation, a large part of the population now understands the role of these immunity boosting supplements and this has led to a significant change in consumer behaviour. Moreover, it has been seen from ancient time that herbal medicines play a great role in preventing diseases and offer better health to human being. India is the origin of “Ayurveda”means “The Science of Life” and is often called the “Mother of All Healing”. Herbal medicine is the basis of “Ayurveda medicine” which comes into limelight over 5000 years ago in India. Many nutraceuticals and functional foods are worth to mention here which are used to develop herbal medicines. Moreover, special micronutrients (Phytochemicals) are discovered which are used to cure many diseases like cardiovascular disease (CVD), cancer, diabetes, dementia etc. Nature is enriched with such ingredients which are progressively discovered by many research groups. Several research works are already pursued to explore the relationships among various food components, their physiological functionality and health benefits. Functional foods contain natural extracts, concentrates, or natural ingredients majorly which can reduce current healthcare costs by health improvement and disease prevention. The past decade has evidenced consumer driven trend of the functional food consumption. Future prospect of this trend is excellent and as a result, scientific information on all aspects of functional food ingredients is important for the advancement of this emerging sector. The second wave of COVID-19 has also paved the way for the nutraceutical sector to build a strong presence in the domestic as well as global market. With an objective to provide better understanding of use, benefits, significance and impact on human health for various nutraceutical foods, natural bioactive compounds and to disseminate the recent developments in such a rapidly expanding field, this book entitled “Functional Foods – Processing and Packaging Aspects” under the publication of the New India Publishing Agency, New Delhi has been compiled and edited. Seventeen chapters in this book not only cover various aspects of functional foods and bioactive compounds from diverse natural sources and its impact, but also discuss the sources and applications of natural antioxidants, probiotics, prebiotics and synbiotics.The contributing authors are experts in their respective fields, and I am grateful to each and every one of them for their thoughtfulness in contributions to this book. This book will be of interest to a wide spectrum of professionals from food scientists and technologists, nutritionists, biochemists, and engineers to entrepreneurs worldwide. It will also serve as a unique reference for food scientists for the R&D departments of food companies that are working with functional foods and ingredients.

Introduction The health and wellness continue to drive growth in the global food industry as the consumers are constantly looking for the foods, beverages and other dietary solutions that will help them more aggressively to meet their own unique nutrition and personal health goals. Worldwide countries are going through a nutritional transition and are now affected by double burden of problems like nutritional and food security. The common alarming problems are under nutrition and specific nutrient deficiencies simultaneously coupled with imbalanced diets and chronic diseases. The problem of chronic diseases, non-communicable diseases (cardiovascular diseases, respiratory diseases, diabetes, obesity, cancer etc.) and malnutrition is more serious in the developing countries. The lack of access to health services, sufficient income, healthy and nutritious foods characterize majority of poor people in developing countries. So, food is not only the solution but also viewed as a product to enhance health and wellbeing, and it encourages the producers by supplying new goods that meet these needs. From the past decade, there has been growing consumer awareness regarding health, nutrition and the food safety which are often triggered by specific food incidents, especially in the developed countries and high-income groups in such countries. In the food industry there have been two broad trends which are hazards in the food chain and nutritional profile of the food. There has been recognition that hazards and contaminants can enter the food chain and can cause sickness, death and diseases, and so governments, often to deal with consumer and industry concerns, establish regulations and standards on food hazards including standards on pesticides, antibiotics, microbiological hazards and other chemical and physical contaminants. On the other hand, there is also a growing awareness of the health-enhancing properties for prevention and treatment of health concerns beyond the basic nutritional component of many foods.

Introduction Society is dynamic. The requirement of society varies in course of time. During the life process human being faces lots of health hazards which are mostly due to intake of various foods. So people become cautious about the intake of food and think about the quality of foods which can protect the health of human being. In ancient times people made medicines from plant extracts to protect their health. In 1989, Dr. Stephen coined the term “Nutraceuticals” which is combination of nutrition and pharmaceutical. Nutrition is required for health and pharmaceuticals provide remedy for sickness. So it is a preventive approach. Nowadays, the definition of nutraceuticals has been expanded to include vitamins, minerals, herbs and other botanicals, amino acids, and dietary substance for human use as a supplement diet (Stauffer, 1999). Nutraceuticals are dietary supplements made only from whole foods to augment health. Currently over 470 nutraceutical and functional food products are available with documented health benefits (Brower, 1999). The patients are much aware about the side effects of chemical agents in short and long term therapy. So to avoid medicine used for small diseases, new therapies came into trend with the help of nutritional approaches i.e. “Nutraceuticals”. The use of nutraceuticals becomes easier than pharmaceuticals since nutraceuticals does not require any animal verification for its use in human. Nutraceuticals, Functional foods and Dietary Supplements are most important concept in food science. They have distinction amongst themselves.

Introduction Our traditional knowledge of medicine has been one of the strongest areas of research because of our rich heritage in herbal plants & great biodiversity in our fauna & flora. Consumption of plant foods and the structurally diverse natural products provided by them have long been associated with health-protective and therapeutic effects. Natural products in plant foods have been shown to have pleiotropic therapeutic and chemopreventive properties. These bioactive products are known to potentiate the efficacy of other drugs and have synergistic effects too. Most of the prescription drugs discovered so far is from plant sources and 75% drugs had been claimed in folklore claims and traditional medicinal system (Reynold, 1991; Benowitz, 1996). The traditional Indian medicinal system utilizes multicomponent formulations of plant extracts which may either act synergistically or avoid many side effects and might have added up trace element through combination of different plant extracts which could have additive effects. However the effort to utilize traditional knowledge in the light of modern scientific findings is lacking in India. Accumulating evidences as well as in-vitro studies implicates inverse relationship between regular consumption of fruits vegetable and the risk of specific cancer. Diet derived nutritional compound mediated interventions in the process of tumorigenesis may be promising chemopreventive strategy with minimal toxicities to the physiological system.

Introduction Consumers are nowadays very much concerned about the food they purchase and consume. Consumer wants the food containing antioxidant properties or can say functional food in safe and stable forms, so that the availability of daily requirements of functional food ingredient must be ensured for normal growth and development of the body. The functional food that can provide a health benefit such as increased dietary fibre, vitamins or minerals to the body. The incorporation of antioxidant compounds acts as an efficient way to fight against reactive oxygen species (ROS) generated in our body. As it is already known that the ROS causes oxidative stress and acts as a main trigger for different life threatening chronic diseases like cancer, diabetes, cardiovascular disease, aging etc. Therefore, seeing the consumers’ demand for value added products with bioactive ingredients, all small and large scale food industries are taking initiatives to process different types of food (fruits, vegetables, dairy, sea food, animal, microbial products etc.) to extract and purify its active fraction of functional ingredients to valorize different value added products. Functional ingredients may also be derived from different food wastes generated during processing of food. This practice provides additional economic benefits to food businesses. Food industries are also giving importance to different processing methods to extract high quality of functional food ingredients in large quantity because processing method affects the concentration of extracted bioactive compounds. The simple addition of functional ingredients may cause an unwanted and negative change of sensory and texture of food. Therefore, using novel processing technologies such as microencapsulation, the negative changes could be minimized or avoided because these technologies could prevent the inactivation of bioactive ingredients.

Introduction Fruits contain various beneficial compounds that possess biological activities and can be used as pharmaceutical nutraceutical products. Most of the fruits contain polyphenolic components that can promote antioxidant potential on human health. Wild Blueberries, strawberries, plums, oranges, grapes, cherries and mangoes are rich in antioxidant. Additionally, anti-inflammatory, antibacterial, antifungal and chemopreventive effects are associated with the various fruits. Besides polyphenols, other compounds such as xanthones, carotenoids, saponins also exhibit health effects. Fruit consumption is considerably increasing in the daily diet because they supply high levels of biologically active compounds that impart health benefits beyond basic nutrition. Fruits provide an optimal mixture of antioxidants such as vitamins C and E, polyphenols and carotenoids, vitamins, phytoestrogens, glucosinolates and anthocyanins. Diets rich in fruits may help to delay the ageing processes and reduce the risk of certain types of cancer, cardiovascular diseases and other chronic diseases. The combination of vitamins, minerals, phenolic, antioxidants and fibre seems to be responsible for these effects. The most dietary components in fruits acting as antioxidants are fibre, polyphenols, conjugated isomers of linoleic acid, D-limolene, epigallocatechin, gallate, soy protein, isoflavones, vitamins A, B, C and E (group of tocopherols), calcium, selenium, chlorophyllin, alipharin, sulphides, catechin, tetrahy-drocurecumin, seasaminol, glutathione, uric acid, indoles, thiocyanates and protease inhibitors.

Introduction Functional elements are non-conventional biomolecules in foods that have the ability to modulate one or more body metabolic processes or pathways, leading in health advantages and well-being promotion. Research has demonstrated a connection between food, health and well-being functional elements. Consequently, functional elements have health-promoting roles connected with multiple progressive steps at various phases of disease control, from initiation to growth. They can therefore be implemented efficiently in disease therapy and prevention. Therefore, the borderline between food and medicine is becoming very thin at a moment when the role of a healthy diet in stopping non-communicable diseases is well recognized. Functional elements include plant-derived, non-nutritious and biologically active chemicals that operate in the body to avoid certain non-communicable diseases from occurring. Food contains over 900 phytochemicals. One fruit or vegetable serving (about 120g) can contain as many as 100 distinct phytochemicals. Previously, functional elements were believed to happen predominantly only as phytochemicals in plant products including whole grains, fruits and vegetables. However, in animal goods such as milk, fermented dairy products and cold water fish, probiotics, conjugated linolenic acid, long-chain omega-3, −6 and −9 polyunsaturated fatty acids and bioactive peptides are also discovered. Usually, functional elements happen in various forms in food such as glycosylated, esterified, thiolated or hydroxylated materials. They also have numerous metabolic operations that enable positive impacts in the body on several illnesses and target tissues (Balic et al. 2020).

Introduction Pulses are annually grown leguminous crops that yield one to twelve grains/ seeds within a pod, and are often promoted in diet owing to their low cost and many beneficial nutritional effects (Craig 2009). The United Nations general assembly has declared 2016 as the international year of pulses (Oyeyinka et al. 2016). The Food and Agriculture Organization (FAO) describes pulses as the crops that are harvested only for dry grain. This definition excludes crops that are harvested green as food sources (such as green beans and green peas), crops that are used only for sowing purposes (such as alfalfa and clover) and crops used primarily for oil extraction (such as peanuts and soybean). India is the largest producer (around 25%) of pulses in the world and the Indian subcontinent covers the leading area in terms of consumption of pulses. It was also suggested that India can also increase the production output by 30%, considering a rise in government support prices for stimulating the growth of pulses and in case of a normal monsoon season (FAOSTAT 2008). The mainly grown pulses in India include mungbean (green gram), chickpea (Bengal gram), urd bean (black gram), lentil (masur), pigeon pea (arhar), cowpea, horse gram and peas (Tiwari and Singh 2012). In the western countries, nowadays pulses are one of the preferred food sources of the population, owing to ideological reasons and interest in vegetarian diet.

Introduction Biological activation of grain or germination is a natural, biological processing techniques and traditional method that can be used to improve the nutritional, functional, and sensory properties of grains. During biological activation of grains, extensive phytochemical changes were reported in a variety of food grains, which reflects a dynamic and complex nutrient flux including remobilization, degradation and accumulation. During this process many hydrolytic enzymes were activated to hydrolyze the starches, proteins and, non-starch polysaccharides, which results in more oligosaccharides and free amino acids. Many nutrients and bioactive compounds reportedly increase and then decrease as they are utilized by the growing plant, which results in degradation of the high molecular weight polymers for generation of bio-functional substances. The biological activation of grains also realizes a significant growth of nutritional value by increasing the bioavailability of nutritional compounds, vitamins, bio-elements and other biologically active substances due to the partial hydrolysis of starch, proteins, hemicelluloses and even celluloses. Biological activation triggers the enzymatic activity in grain and dormant hydrolytic enzymes are activated, breaking down starch, fibers and proteins and leading to an increase in the amount of digestible compounds along with improvement of functional properties without any chemical modification. Also, the activity of some anti-nutritional factors (enzyme inhibitors, haemagglutinins, anti-vitamins etc.) decreases or disappears during activation, allowing a complete valorization of biological compounds in grains. During biological activation hydrolytic amylase enzymes, proteolytic enzymes, and catalase were activated in grains, which enhances the starch degradation, a complex biochemical process and thus lead to the conversion of insoluble granules to soluble starch and dextrin. Apart from positive shifting in the level of nutrition, biochemical activities which occur during activation also generate bioactive components of health-promoting activities through the enzymatic actions that are antioxidants in nature, such as ascorbic acid, tocopherols, tocotrienols and phenolic compounds, thus resulting in an increase of antioxidant activity.

Introduction India is considered as the “Home of Spices”. They are a prized group of minor components and have been an integral ingredient of Indian diet. Since antiquity, spices form an important part of the Ayurvedic Pharmacopoeia (The Indian System of Medicine), with their widespread traditional use. With its wide climatic conditions and topographical features, India naturally possesses wide variety of medicinal flora. The country enjoys the distinction of being the largest producer and consumer of spices as well as the fastest growing spice market in the world. Spices are potent plant foods that possess a wide range of nutrient bioactive compounds that contribute to improve health, while adding variety, colour, flavour and aroma to daily diet. The term spice or condiment applies to “such natural plant or vegetable products or mixtures thereof, in whole or ground form, as are used for imparting flavour, aroma and piquancy to and for seasoning food” (Sikri and Berwal, 2008). Webster (2011) describes spices as ‘Any of various aromatic vegetable productions as pepper, cinnamon, nutmeg, mace, allspice, ginger, cloves, etc., used in cookery to season and to flavour sauces, pickles, etc.; a vegetable condiment or relish, usually in the form of a powder; also, as condiments collectively’.

Introduction Food ingredients sector is indicating ample prospects for growth. The segment is an obligatory element for the growth of the food and beverage sector. These ingredients are also driving the development of the healthy functional foods industry to increase by 20% growth annually. Innovation in the food and beverage market, especially in the processed food domain, has created innumerable opportunities for new product development in the ingredients market as well. The segment is driven by the growing food processing industry and India is emerging as ingredients bowl of the future. Additionally, growing urbanization coupled with industrialization and changing lifestyles have led to growing demand for convenient food that offers health benefits, over and above the basic nutritional benefits of the foodstuff. There are nearly 10 to 15 broad classes of ingredients that include the very basic ingredients as well as certain value added ingredients that have niche applications. Key ingredients are preservatives, flavours, colours, hydrocolloids/stabilizers, emulsifiers, artificial sweeteners and value added health food ingredients such as omega-3-fatty acids prebiotics, probiotics etc. Until recently, there has been limited research into functional foods in meats, while functional foods in the dairy sector have been developed to a high degree. But meat is less allergenic than many other foods and much more research into the development and marketing of novel functional meat products is required. Such meat products would open up a new market for the meat industry. Nutraceutical and functional food industry is a very vibrant industry today with the claims and a clear mandate of health, wellness and prevention of diseases as one of its main goals.

Introduction Increasing knowledge regarding the impact of diet on human health along with state-of-the-art technologies has led to significant nutritional discoveries, product innovations, and mass production on an unprecedented scale. In particular, naturally occurring bioactive extracts or single compounds thereof, that are believed to benefit human health, have spawned an important and dynamic new area of research resulting in substantial advances in nutritional knowledge. There is also growing awareness that dietary source and form of food may affect overall health. Suitably, the role of food as an agent for improving health has been recognized, initiating the development of new classes of food, known as functional foods (Honkanen 2009). The concept of functional foods is to improve the general conditions of the body and decrease the risk of illness and disease. That is to say, bioactive compounds present as natural constituents or as fortificants in food having the potential to provide health benefits beyond the basic nutritional value of the product. Marine-derived nutrients and other marine bioactive components have excellent potential as functional food ingredients as they possess advantageous physiological effects, with medicinal characteristics and added health benefits such as anticancer or anti-inflammatory activity. The marine world, due to its phenomenal biodiversity, is a rich natural resource of many biologically active compounds such as polyunsaturated fatty acids (PUFAs), sterols, proteins, polysaccharides, antioxidants and pigments. Many marine organisms live in complex habitats exposed to extreme conditions and, in adapting to new environmental surroundings, they produce a wide variety of secondary (biologically active) metabolites which cannot be found in other organisms. Moreover, considering its great taxonomic diversity, investigations related to the search of new bioactive compounds from the marine environment can be seen as an almost unlimited field.

Introduction In recent years, food scientists, nutritionists and health experts have become more inclined towards functional food components present in various food sources due to their positive influence on health, which is beyond providing the basic nutrition.Nowa days, generalconsumers are also seeking healthier food options because of nutritive food habits and awareness. Therefore, it has triggered the studyof food and deep mining of food components from nutritional and health benefit’s perspective also. Functional food ingredient can be defined as any componentwhich is more than a source of nutrient i.e. provides additional specific health benefits. Milk is well known to exert positive effect on human health, provides nutrition and widely consumed all across the globe in form of either milk or milk products. As per the definition, milk is heterogeneous blend of lacteal secretion which contains various elements that displays wide range of chemical and functional activities. It has been more than a source nutrition and supplement to different age groups either neonates, youngsters or grown up peoples (Park, 2009). It is one of the basicelements of many diets either in its native form or fermented form which every class of people can afford and had evolved as one of the important nourishment sources. Milk in its native form contains fewer bioactive components while fermentation process produces numerous secondary metabolites which possess various health-beneficial properties (Stanton et al., 2005).

Introduction During the first 50 years of the 20th century, scientific focus was on the identification of essential elements and their role in the prevention of various dietary deficiency diseases. This emphasis on nutrient deficiencies or “undernutrition” shifted dramatically, however, during the 1970s when diseases linked to excess and “overnutrition” became a major public health concern. “Let food be thy medicine and medicine be thy food”, the age old quote by Hippocrates is the ideology of today’s health conscious population. Eli Metchnikoff, the Russian Nobel prize winner was the first one to recognize the beneficial role of selected bacteria on gastrointestinal tract of humans. Subsequently the “Theory of Longevity” by Metchnikoff was correlated with prolonged youth and a healthy old age, observed largely in Balkan peasants of those times, who used cultured milks in their diet (Kaufmann, 2008). Since then, the quest continues for understanding the role of wide range of food components and nutrients in enhancing health or preventing chronic diseases. The research in this field has resulted in a plethora of new labels for pro-, pre- and synbiotics.

Introduction Hippocrate, an ancient physician, a great thinker and also often referred as father of medicine, for the first time conceive the idea nearly 2,500 years ago that a disease is caused due to natural factors such as environmental factors, diet, and living habits. He advocated the secret of healthy living in healthy food in his famous quote “Let food be thy medicine and medicine be thy food”. But unfortunately, this wisdom about food has faded with the time and we became dependent on synthetic medicines and drugs for even life style disorders. In the last century, research advancement in the field of food science and nutrition established food not only as a source of nutrition but also a source of active food components that aid specific functions and rejuvenated the Hippocrates concept. According to the Department of Health and Human Services, USA, daily diet of a person, plays a crucial role in 5 of 10 diseases which are the leading causes of death, such as diabetes (noninsulin dependent or type 2), stroke, coronary heart disease (CHD), specific type of cancer, and atherosclerosis. In the mid-1980s, term “functional food” was introduce in Japan and defined as food that contain active components either from plant or animal origin and provide physiological benefits in addition to the basic nutritional needs.

Introduction The art of fermentation process is developed as far back as thousand years ago without understanding the role of microorganism in it. It is believed that the process of fermentation originates in Indus valley during 7000 – 8000 BC. Archives of harappan civilization showed that different clay utensils were used for the fermented product and the agriculture and animal husbandry were fully developed during that period. It is evident from the artifacts of Egypt and middle east that the practice of fermentation was common in that part (Farnworth, 2003). The earliest practice of cheese making is originated from Falcate between Tigris and the Euphrates rivers in Iraq, 8000 years back. Later, Egyptians developed the use of yeast in wine and bread making during 4000 BC (Ross et al., 2002). Further, Sumarians prepared beer from fermented barley during 1750 BC. Earlier the fermenting process was an artisan way without any knowledge of microorganism. However, the main turning point was in 1608, when Antony Van Leeuwenhoek first established the use of a microscope and revealed the existence of microorganisms. Another, major finding was Pasteurization by Louis Pasteur in 1861 AD and this was the first report that documented the role of microorganism in fermentation. Later, there was a shift observed in the production of fermented food from local communities to large scale industry. The health effect of fermented food was first reported by Roman historian Pliny, who reported that fermented milk is beneficial for gastrointestinal complains. Further, a French paediatrician proved that Bifidobacteria in food prevents infants against infection (Grill et al., 1995).

Introduction COVID-19, a worldwide pandemic, addresses a deflationary stun for the worldwide economy. The outbreak of coronavirus has had a significant impact on public health worldwide and all stages of various industries’ supply chain and value chain. The 2019 novel coronavirus (2019-nCoV) or the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is rapidly spreading from its origin in Wuhan City of Hubei Province of China to the rest of the world. The infection began in bats and was communicated to people by inhalation or contact with infected droplets, and its infection time frame goes from 2 to 14 days. Primary manifestations in hospitalized patients with COVID-19 include fever (98%), cough/hack (76%), and myalgia or weakness (44%). Severe complications include acute respiratory distress syndrome (ARDS), RNAemia, acute cardiac injury, and multiple organ failure. However, exaggerated immune responses may lead to Cytokine Storm. Cytokine storm syndrome includes ARDS, shock, and multi-organ failure (Zheng et al., 2020). Many people are asymptomatic also, and the severity of this disease varies from person to person. The fatality rate is estimated to range from 2 to 3%. Typical/low white cell checks can analyze it with raised C-responsive protein (CRP), periodic modernized tomographic chest examination. The infection spreads quicker than its two predecessors, the SARS-CoV and Middle East respiratory disorder Covid (MERS-CoV); however, it has lower casualty. The worldwide effect of this new scourge is yet questionable (Singhal, T. (2020).

Introduction Food and nutraceuticals are obtained from various agricultural resources available in this planet. Many types of bio-active compounds are available in small quantities in the plants and animals which are evidenced to improve performance of various organs, increase in immunity and cure for some diseases. Last decade evidences several advancements in developing phytochemical formulations which can be used as a cure for many diseases as well as helps in boosting immunity in human being. Such materials exist in different form, structure and chemical compositions. Examples of such chemicals are phenolic and organosulphur compound (Pascall 2011). The vegetables, fruits and seeds are used without further processing. Some plants are subjected to cooking before its consumption. Many industries are carried out extraction of bioactive compounds and developed products in the form of tablets, powders and liquids which are available in the market. In order to make commercial process more economic and feasible, long term storage of plant residues (Raw materials) as well as the prevention of the extracts emerges as essential requirement for the industries. Moreover, the products requires suitable packaging solution to increase its self-life, preventing it from the influences of environment, and to make viable solution for easy access which makes it more user-friendly. Suitable packaging solution is required for mainly the three reasons: (i) prevention. (ii) protection and (iii) presentation. Prevention is required in-order to avoid deterioration of the packaged items due to microbial growth, loss in moisture and enzymatic action. Protection is required for the purpose of protecting the packaging materials from the contamination as well as from the environment impact and rough handling. Presentation is equally important to provide it an aesthetic look to make it more approachable to the consumers. Moreover, packaging also offers “labelling for easy identification, warnings, instructions, and nutritional information, ease of transportation” (Pascall 2013).