This manual can be utilized by undergraduate and postgraduate students in various fields of biological sciences. It offers detailed, step-by-step guidance for conducting laboratory experiments. This manual will also be helpful for researchers and students planning experiments in a molecular biology laboratory.

The authors have extensive experience in molecular biology and are actively engaged in research and teaching in this area. At the end of each experiment, a set of questions has been included to help students better understand the topic. This manual also includes a set of objective questions related to molecular diagnostics that will be beneficial for both theoretical and competitive exams.

Diwakar Singh: Assistant Professor (Biochemistry), Department of Plant Molecular Biology and Biotechnology,, ASPEE College of Horticulture and Forestry,, Navsari Agricultural University, Eru Char Rasta, Dandi Road, Navsari - 396 450, Gujarat – India

K.P. Suthar: Assistant Professor, ASPEE College of Horticulture and Forestry, Navsari Agricultural University, Eru Char Rasta, Dandi Road, Navsari - 396 450, Gujarat – India

Reetu Mehta: Lecturer, Naranlala College of Professional and applied Sciences, Navsari-396450, Gujarat, India

Diagnosis of Drought Tolerance in Crop Plants by PEG Method

Identification of Salt Tolerant Crop Variety by Glycine Betaine (GB) Method

Total Protein Estimation by Folin-Lowry’s Method

Protein Electrophoresis Using SDS-PAGE

Enzyme Extraction and Assay Study of Polyphenol Oxidase (PPO; EC 1.14.18.1)

Enzyme Extraction and Assay Study of Catalase (EC 1.11.1.6)

Enzyme Extraction and Assay Study of Guaiacol Peroxidase (POX) (EC 1.11.1.7)

Enzyme Extraction and Activity Study of Superoxide Dismutase (EC 1.15.1.1)

Peroxidase Isozyme Study by Electrophoresis Method

DNA Isolation from Plant Sample and Quantification by Spectrophotometer

Quality Check of Plant Genomic DNA on Agarose Gel Electrophoresis

Randomly Amplified Polymorphic DNA (RAPD) and Electrophoresis

Southern Blotting Experiment

Total RNA Isolation from Plants and its Quantification by Spectrophotometer

Quality Check of Total RNA on Agarose Gel

cDNA Synthesis from Isolated Plant Total RNA

Indirect Competitive ELISA

Objective Questions