Laboratory Manual for Immunology
authored by: Amit Kumar
Laboratory Manual for Immunology is a practical and hands-on manual designed to support undergraduate teaching and laboratory training in immunology. The book has been prepared in the context of the ICAR 6th Dean Committee recommendations and the revised B.Tech. Biotechnology curriculum, in which Immunology is offered during the third year. It provides students with fundamental practical knowledge of immunological procedures, laboratory techniques and serological reactions.The manual begins with essential laboratory procedures, including handling of experimental laboratory animals, methods of inoculation and blood collection, followed by the preparation of bacterin for raising polyclonal serum. It then progresses to important concepts and practical techniques involving antigen–antibody interactions, agglutination reactions, precipitation reactions, agar gel immunodiffusion (AGID), radial immunodiffusion (RID), ELISA, SDS-PAGE, Western blotting and radioimmunoassay.
The book also connects laboratory techniques with their diagnostic and research applications, including detection of antibodies and antigens, diagnosis of infectious diseases, identification and classification of microorganisms, and quantitative estimation of antigen or antibody concentrations. The final section provides an outline of vaccines and vaccine delivery approaches, giving students an introduction to the broader application of immunological principles in disease prevention.
Prof. Amit Kumar is a Professor and Head of the Division of Animal Biotechnology at the College of Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Modipuram, Meerut, Uttar Pradesh, India. His academic and professional responsibilities involve teaching, research, and academic leadership in the field of animal biotechnology.
Chapter 1. Selection, Care and Handling of Laboratory Animals
Chapter 2. Methods of Inoculation in Experimental Laboratory Animals
Chapter 3. Methods of Collection of Blood in Lab Animals
Chapter 4. Preparation of Bacterin to Raise Polyclonal Serum
Chapter 5. Preparation of Immune Serum for Different Serological Reaction
Chapter 6. Separation of Blood Cells
Chapter 7. Agglutination Reaction
Chapter 8. Precipitation Reaction
Chapter 9. Agar Gel Immuno Diffusion Test
Chapter 10. Radial Immuno-Diffusion (RID) Reaction
Chapter 11. Enzyme-linked Immunosorbent Assay (ELISA)
Chapter 12. Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)
Chapter 13. Western Blotting/Immunoblotting
Chapter 14. Radio Immuno Assay (RIA)
Chapter 15. Immunohistochemistry
Chapter 16. Vaccine: Its Type and Method of Production
Immunology laboratory manual, practical immunology, immunology techniques, veterinary immunology, animal immunology, laboratory immunology, antigen antibody reactions, serological techniques, agglutination reaction, precipitation reaction, agar gel immunodiffusion, radial immunodiffusion, ELISA, SDS-PAGE, Western blotting, radioimmunoassay, immunohistochemistry, vaccine production, antibody production, immunological diagnostics.