Molecular Breeding and Biotechnology Techniques for Fruit Crop Improvement provides a comprehensive account of modern molecular and biotechnological approaches for improving fruit crops. The book brings together recent developments in molecular breeding, genomics, phenomics, bioinformatics, functional genomics, biotechnology, artificial intelligence, genome editing, RNA technologies, epigenetics and nanobiotechnology.

The volume begins with the genetic architecture of yield, fruit quality and stress tolerance, followed by high-throughput phenotyping, molecular markers, genotyping platforms, QTL mapping, GWAS, marker-assisted selection and genomic selection. It subsequently covers genome sequencing, multi-omics, disease resistance, abiotic-stress tolerance, climate resilience, machine learning and AI-assisted breeding.

The later chapters focus on tissue culture, micropropagation, somatic embryogenesis, cryopreservation, synthetic seeds, genetic transformation, cisgenesis, CRISPR/Cas genome editing, RNA-based technologies and epigenetic regulation. The book concludes with rootstock–scion interactions and applications of nanobiotechnology and nanomaterials in fruit crop improvement.

The book is particularly relevant to fruit breeders, horticulturists, plant geneticists, molecular biologists, biotechnologists, genomics researchers, postgraduate students, agricultural scientists and professionals involved in developing high-yielding, high-quality, disease-resistant and climate-resilient fruit cultivars.

Chapter 1. Genetic Architecture of Yield, Quality, and Stress Tolerance Traits in Fruit Crops

Chapter 2. High-Throughput Phenotyping and Trait Evaluation in Fruit Crops

Chapter 3. Molecular Markers, Genotyping Platforms, and Bioinformatics Tools

Chapter 4. QTL Mapping and Genome-Wide Association Studies in Fruit Crops

Chapter 5. Marker-Assisted Selection and Genomic Selection in Fruit Crops

Chapter 6. Genome Sequencing Technologies in Fruit Crops

Chapter 7. Functional Genomics and Multi-Omics Approaches in Fruit Crops

Chapter 8. Molecular Breeding for Disease

Chapter 9. Molecular Breeding for Abiotic Stress Tolerance and Climate Resilience in Fruit Crops

Chapter 10. Machine Learning and Artificial Intelligence in Molecular Breeding

Chapter 11. In Vitro Culture, Micropropagation, and Quality Planting Material Production

Chapter 12. Somatic Embryogenesis, Cryopreservation, and Synthetic Seed Technology

Chapter 13. Genetic Transformation and Cisgenic Approaches in Fruit Crops

Chapter 14. Genome Editing Technologies in Fruit Crops

Chapter 15. RNA-Based Technologies and Epigenetic Regulation in Fruit Crops

Chapter 16. Epigenetic Mechanism of Abiotic Stress Responses in Fruit Crop

Chapter 17. Epigenetic Mechanism of Biotic Stress Responses in Fruit Crop

Chapter 18. Rootstock Breeding and Scion–Rootstock Interactions

Chapter 19. Nanobiotechnology and Nanomaterials in Fruit Crop Improvement