Enzymology and Enzyme Technologies provides a structured introduction to enzymes, their biological significance, molecular structure and practical applications. The book explains enzyme classification, nomenclature, active sites, cofactors, coenzymes and metalloenzymes before progressing to enzyme isolation, purification, assays and characterization. It presents the essential principles of enzyme kinetics, including Michaelis–Menten kinetics, Km, Vmax and the effects of pH and temperature. The regulation and inhibition of enzyme activity are discussed alongside isoenzymes, schizomers and isoschizomers. Advanced topics include ribozymes, catalytic RNA and enzyme immobilization. By connecting fundamental enzymology with applications in biotechnology, agriculture, medicine, food processing, industry and environmental science, the book serves as a useful academic resource for undergraduate and postgraduate students of biochemistry, biotechnology, microbiology, food science and related life-science disciplines.