Search in
Keywords

Restriction endonucleases (Type II / IIS), Recognition sequence & overhangs (sticky vs blunt),
DNA ligase (T4) & ligation efficiency, Alkaline phosphatase (vector dephosphorylation), Multiple Cloning Site (MCS), Origin of replication (ori) & copy number, Selectable markers (Amp^R, Kan^R),
Reporter systems (lacZ/blue–white), Plasmids, Cosmids, Phagemids, BAC/PAC/YAC vectors,
Shuttle & co-integrating vectors, Transposon vectors, Competent cells (CaCl₂ heat-shock),
Electroporation, ,Gene cloning & library construction (genomic/cDNA),Probe preparation & labeling (radio/chemiluminescent), Gel electrophoresis (agarose/PAGE), Southern / Northern / Western blotting, PCR & variants (RT-PCR, qPCR, Nested), Gibson Assembly / Golden Gate (Type IIS), ICAR 6th Deans Syllabus

Recombinant DNA Technology - Volume 04

Language: English | Imprint: NIPA

Hardback

ISBN: 9789367552582
Pages: 200 | Length: 152 mm | Breadth: 12 mm | Height: 229 mm | Weight: 600 GSM
Print Book Price: 200.00 USD (Get 10% OFF)
Shipping:
Free Worldwide Delivery Within 10-15 Days By Indian Post (Traceable Methods)

EBook

EISBN: 9789367550120
Ebook Individual Price: 0.00 USD
Individual Rates Only:
Access valid for 30 days from the date of activation for single user only.

eChapter

eChapter details: Available in the Content section below.
<p style="text-align:justify">The book is&nbsp;in accordance with the recommendations of the <strong>ICAR Sixth Deans&#39; Committee and NEP-2020</strong>, <em>Fundamentals of Recombinant DNA Technology</em> provides a comprehensive, student-friendly, and outcome-based introduction to the principles and applications of modern molecular biology and genetic engineering. The book is designed to equip undergraduate students with the conceptual understanding and practical competencies required in contemporary agricultural, biological, and biotechnological sciences.</p> <p style="text-align:justify">Beginning with the structure and properties of nucleic acids, the text systematically covers restriction enzymes, cloning vectors, recombinant DNA methodologies, gene transfer techniques, polymerase chain reaction (PCR), genomic and cDNA libraries, DNA sequencing, molecular markers, and gene expression systems. Advanced topics such as transgenic technologies, genome editing, biosafety, bioethics, and regulatory frameworks are integrated to connect fundamental concepts with emerging developments in life sciences and agriculture.</p> <p style="text-align:justify">The subject matter is presented in a simple and logical manner through illustrations, flowcharts, comparative tables, and practical examples that promote conceptual clarity, analytical thinking, and skill development. Special emphasis has been placed on laboratory techniques, experimental design, interpretation of molecular data, and problem-solving approaches to support experiential learning and competency-based education.</p> <p style="text-align:justify"><strong>Salient Features</strong></p> <p style="text-align:justify">&bull; Fully aligned with the <strong>ICAR Sixth Deans&#39; Committee syllabus and course outcomes</strong>.<br /> &bull; Outcome-based and competency-driven approach in accordance with <strong>NEP-2020</strong>.<br /> &bull; Comprehensive coverage of the principles and applications of recombinant DNA technology and genetic engineering.<br /> &bull; Student-friendly presentation through illustrations, flowcharts, diagrams, and summary tables.<br /> &bull; Emphasis on laboratory skills, molecular techniques, and interpretation of experimental results.<br /> &bull; Includes chapter-end review questions, objective questions, and self-assessment exercises.<br /> &bull; Integrates contemporary topics such as genome editing, transgenic technologies, biosafety, and bioethics.<br /> &bull; Useful for classroom teaching, practical training, experiential learning, and competitive examination preparation.</p> <p style="text-align:justify">This textbook is primarily intended for <strong>undergraduate students of Agriculture, Biotechnology, Horticulture, Forestry, and allied life sciences</strong>, and will also serve as a valuable reference for postgraduate students, teachers, researchers, and professionals interested in molecular biology and genetic engineering.</p>
Payment Methods