Computational and Systems Approaches in RNA Therapeutic Design presents an interdisciplinary approach to the rapidly developing field of RNA-based medicine, bringing together RNA biology, bioinformatics, computational biology, artificial intelligence, machine learning, systems biology and drug discovery. Computational methods are increasingly important for RNA therapeutic development, including RNA structure prediction, target identification, molecular modelling, sequence design and delivery optimization.

The book begins with the fundamentals of RNA therapeutics, RNA structure, function and stability, before progressing to computational tools for RNA folding and structure prediction and databases supporting RNA research. It explores AI and machine learning in RNA drug discovery, in-silico design of siRNA, miRNA and mRNA therapeutics, molecular docking and molecular dynamics, and systems-level analysis of RNA regulatory networks.

Dr. Mohd. Tariq is a multidisciplinary biotechnologist, academic administrator, and researcher with extensive expertise in plant-derived bioactive compounds, secondary metabolites, and their therapeutic applications. He currently serves as Associate Professor at Sumandeep College of Allied and Healthcare Sciences and Assistant Registrar (Academics) at Sumandeep Vidyapeeth Deemed to be University, Vadodara, India, where he contributes to academic governance, quality assurance, and regulatory frameworks, while actively engaging in research and scholarly publishing.

Dr. Tariq earned his Ph.D. in Biotechnology from Kumaun University, Uttarakhand, with doctoral research focused on biotechnological and ecological strategies for the conservation of Paris polyphylla, a high-value medicinal plant of the Indian Himalayan Region. His academic foundation also includes an M.Sc. in Biotechnology from Graphic Era University and a B.Sc. (Hons.) in Zoology from the University of Delhi. His research interests broadly encompass plant secondary metabolites, ethnopharmacology, metabolomics, neuroprotective phytochemicals, nanotechnology-based drug delivery, and plant biotechnology, with increasing emphasis on plant-derived interventions for neurological and neurodegenerative disorders.

Dr. Tariq has authored and co-authored 100+ peer-reviewed research articles and book chapters published in leading international journals such as Biomedicine & Pharmacotherapy, Scientific Reports, Journal of Ethnopharmacology, Frontiers in Medicine, and Industrial Crops and Products. His scholarly contributions bridge plant biochemistry, neurotherapeutics, gut–brain axis research, nanotechnology, and translational biomedical sciences. He has also edited and contributed to several academic books with publishers such as Springer Nature, Wiley-Scrivener, Elsevier, IGI Global, CRC Press, and Bentham Science.

Dr. Mohd. Tariq is a multidisciplinary biotechnologist, academic administrator, and researcher with extensive expertise in plant-derived bioactive compounds, secondary metabolites, and their therapeutic applications. He currently serves as Associate Professor at Sumandeep College of Allied and Healthcare Sciences and Assistant Registrar (Academics) at Sumandeep Vidyapeeth Deemed to be University, Vadodara, India, where he contributes to academic governance, quality assurance, and regulatory frameworks, while actively engaging in research and scholarly publishing.

Dr. Tariq earned his Ph.D. in Biotechnology from Kumaun University, Uttarakhand, with doctoral research focused on biotechnological and ecological strategies for the conservation of Paris polyphylla, a high-value medicinal plant of the Indian Himalayan Region. His academic foundation also includes an M.Sc. in Biotechnology from Graphic Era University and a B.Sc. (Hons.) in Zoology from the University of Delhi. His research interests broadly encompass plant secondary metabolites, ethnopharmacology, metabolomics, neuroprotective phytochemicals, nanotechnology-based drug delivery, and plant biotechnology, with increasing emphasis on plant-derived interventions for neurological and neurodegenerative disorders.

Dr. Tariq has authored and co-authored 100+ peer-reviewed research articles and book chapters published in leading international journals such as Biomedicine & Pharmacotherapy, Scientific Reports, Journal of Ethnopharmacology, Frontiers in Medicine, and Industrial Crops and Products. His scholarly contributions bridge plant biochemistry, neurotherapeutics, gut–brain axis research, nanotechnology, and translational biomedical sciences. He has also edited and contributed to several academic books with publishers such as Springer Nature, Wiley-Scrivener, Elsevier, IGI Global, CRC Press, and Bentham Science.

Dr. Ayush Madan earned his Ph.D. in Biotechnology from Shobhit University, India. He is currently affiliated as an Assistant Professor at Department of Biotechnology, People’s University, Bhopal, India. Previously, He worked as a Graduate Research Assistant at Universiti Malaysia Terengganu, Malaysia, where he contributes to projects on nanotechnology-driven bioremediation and sustainable agriculture. He completed his M. Tech. in Biotechnology from Graphic Era University, India. Dr. Madan’s academic background includes research at Biocon Research Limited and ONGC, with expertise in microbiology, bioinformatics, and nanobiotechnology. He has published several book chapters with prominent international publishers, focusing on topics such as environmental sustainability, cancer immunotherapy, and AI-driven recycling technologies. His research emphasizes the intersection of biotechnology and environmental health.

Sumel Ashique has been working as an assistant professor at Department of Pharmaceutical Technology, School of Health and Medical Sciences, Adamas University, Kolkata-700126, West Bengal, India. He has 6 years of teaching experience. He has achieved 200 publications of International and national accredited reputed journals. He has knowledge in drug delivery, nanotechnology and targeted treatment strategy. He is working as potential reviewer in many well-known publishers (Elsevier, Springer, Dove Press, and Bentham Sc.). He has also 5 granted patents from IP and Australia, 20 published book chapters in Bentham, Springer, Elsevier, Bentham and Taylor & Francis. Currently is editing 30 books under CRC Press, Elsevier, Taylor and Francis, Wiley, Nova Science and Springer. He has published 80+ papers including research, reviews, column article, editorial in Scopus, WoS indexed. He is serving as guest editor in JoVE journal (IF 1.2, Scopus & WOS indexed), Current Probiotics (Bentham Science) & Current Indian Science Journal. He is a potential reviewer in Elsevier, springer, Taylor & Francis and wiley journal for the expertise in the field of targeted drug delivery, nanotechnology, gut-microbiome, postbiotics. Currently he is pursuing his PhD degree from Lovely Professional University, Punjab, India (NAAC A⁺⁺) in the Pharmaceutical Science department. He has been selected top 2% scientist in the year of 2025 by Stanford University.

Dr. Ajay Kumar is a Professor of Biotechnology, Dean (Research & Development) at Rama University Uttar Pradesh, Kanpur, India, with more than 21 years of experience in teaching, research, and academic leadership. He earned his M.Tech. in Biotechnology from the Institute of Engineering and Technology (IET), Lucknow, and his Ph.D. from the ICAR–Central Institute for Research on Goats (CIRG), Mathura. His expertise spans Genomics & Proteomics, Bioprocess Engineering, Bioinformatics, Microbiology, Genetic Engineering, Environmental Biotechnology, Fermentation Technology, and Food Biotechnology. His research interests include computational vaccine and drug development, cancer biology, genomics, proteomics, and fermentation technology. Dr. Kumar has made significant scholarly contributions with over 130 research publications, 55 book chapters, 6 books, and 7 patents to his credit. He has also served as Chief Editor, Associate Editor, Editorial Board Member, and Reviewer for several reputed international journals. A dedicated research mentor, he has successfully supervised numerous M.Tech. and Ph.D. scholars. He is actively involved in academic governance as a member of the Board of Studies and Academic Council of Rama University and contributes to professional bodies such as IAENG and INSA, while also providing consultancy services in vaccine research and development.

Chapter 1. Overview of RNA Therapeutics: From Concept to Clinic
Sarmita S. Jana, Vasundhara Raina, Singh Khushi Sanjeev, Joshua C. Varughese, Rishabh Garg

Chapter 2. RNA Biology: Structure, Function, and Stability
Hansa Gul, Haris Ahmed Khan, Nouman Ahmad, Muhammad Nauman Khan, Muhammad Iqbal, Mujeeb ur Rahman

Chapter 3. Computational Tools for RNA Structure Prediction and Folding in Therapeutics

Chapter 4. Databases and Repositories for RNA Therapeutics
Uchakankhi Kashyap, Hemanga Kashyap, Nima D. Namsa

Chapter 5. Machine Learning and Artificial Intelligence in RNA Drug Discovery
Bhavya Mudgal, Poornika Sah, Sugam Gupta, Devvret Verma, Kumud Pant, Mohd Tariq, Saleha Diwan, Ayush Madan

Chapter 6. In Silico Design of siRNA, miRNA, and mRNA Therapeutics
Anshul, Sugam Gupta, Devvret Verma, Kumud Pant, Deeksha Kumari, Debasis Mitra

Chapter 7. Molecular Docking and Dynamics in RNA-Targeted Drug Development
Anu Radha Pathania, Aashvi Panchal, Imran Hasan, Anshita, Neha Govind Rao

Chapter 8. Systems Biology Models in RNA Regulatory Networks
Anshul, Devvret Verma, Kumud Pant, Deeksha Kumari, Debasis Mitra, Mohd Tariq, Mohammedtarik Saiyad, Ayush Madan

Chapter 9. Network Biology of RNA Interactions: Deciphering Pathways and Molecular Crosstalk in Disease
Hansa Gul, Mamoona Uzma Kalsoom, Muhammad Iqbal, Muhammad Nauman Khan

Chapter 10. Multi-Omics Integration for RNA Therapeutic Target Identification

Chapter 11. Predictive Modeling for RNA-Based Gene Regulation
Rishabh Garg, Ayush Madan, Mohd Tariq

Chapter 12. Synthetic Biology and Systems-Level RNA Circuit Design

Chapter 13. RNA Delivery Systems Including Lipid Nanoparticles, Exosomes, and Polymers for Therapeutic Application

Chapter 14. Computational Pharmacokinetics and Toxicity Modelling of RNA Drugs
Bishakha Thakur, Ramandeep Saini, Isha Agarwal, Shreya Priyam, Ayush Madan

Chapter 15. Personalized Medicine and RNA Therapeutic Optimization

Chapter 16. Case Studies: mRNA Vaccines and Beyond
Sameel Hassan, Amna Khalil, Muhammad Anas, Anwer Usma, Javed Iqbal, Banzeer Ahsan Abbasi

Chapter 17. Ethical and Regulatory Aspects in RNA Therapeutics

Chapter 18. Data-Driven Innovation in RNA Drug Development

Chapter 19. The Future of AI and Systems Medicine in RNA Therapeutics
Muhammad Nauman Khan, Muhammad Iqbal, Hansa Gul, Zahida Nasreen, Alevcan Kaplan, Mukul Jain, Mohd Tariq