Milk is nature’s perfect food; recommended to all age groups for a healthy body. Developing countries are facing scarcity of milk especially in urban areas. This may be due to the factors like lack of processing facilities and application of advanced processing methodologies. In recent times dramatic changes have taken place in dairy industry by adopting latest technologies. New technologies are emerging with advancement of scientific knowledge in dairying. At present there are books on various processing techniques, quality control methods, but are scattered across several books.

Milk is considered to be a perfect food which contains almost all the macroand micronutrients required for normal body growth. However, it is highly perishable in nature which poses problem during its storage. It gets spoiled within few hours after milking if not processed or cooled immediately. It can also be a source of several disease causing microbes as it is conducive for the growth of microorganisms.

High-pressure processing (HPP) is a novel method for non-thermal preservation technique of food that inactivates harmful pathogens and vegetative spoilage microorganisms. HPP is an alternate, non-thermal food processing method, wherein the food is subjected to a very high pressure range from 100 -800 MPa (1MPa = 145.03 Psi or 10 Bar). HPP is also referred as High hydrostatic pressure processing, Ultra high-pressure processing, Hyperbaric processing and Pascalization (Rao et al., 2014).

Electricity is one of the fundamental phenomena of nature. Electricity or electric current is caused due to the movement of charged particles i.e., electrons. Due to the potential difference between two points in a conductor, electron movement takes place. The potential difference between two points is caused due to the difference in charge density. Electron tends to flow from lower potential to higher potential which causes electricity to flow. In general, the electrons flow from cathode (negative) to anode (positive) but in a wire electricity, electrons flow from anode to cathode when connected to a battery or any electricity source.

Thermal heating methods are used from long time to decontaminate the food products from microorganism and therefore increase its shelf life and ultimately make the product safe for human consumption. But these methods diminish the sensory, physicochemical and nutritional properties of food (Barba et al., 2012). Also, the cost of generation of heat in manufacture of food product make the product costlier in thermal processing and also create environmental issue.

The last two decades have seen rapid growth and development in the industrial sector as well as urbanization has increased. Due to globalization and mass production of commodities, the situation in the modern world nothing like ever before. Food and dairy industries are also not left out from this massive expansion. Due to changing behavior and food consumption habits too, there has been a surge in the risk of foodborne illness. This has created a problem in terms of public health and sustainability

Biosensors are the next generation detection devices, use biological sensing elements like enzymes, nucleic acids, antibody, cells, organelles, tissues, receptors; have got varied applications in the field of drug discovery, diagnosis, biomedicine, food safety and processing, environmental monitoring, defense and security etc. Microbial biosensors are analytical device that uses naturally occurring or engineered microbial cells as the recognition elements to detect physiological changes in cells with the help of suitable physicochemical transducer.

Fourier transform infrared (FTIR) spectroscopy is one of the most effective methods for determining the functional groups and potential molecular interactions between membrane chemical molecules. Understanding of (infrared) IR absorption band placements in the spectrum as wave numbers can be used to identify the various chemical constituents (such as aromatic amides) that may not be visible by X-ray photoelectron spectroscopy.

Enzymes (biological catalyst) can be defined as a substance which initiates or fastens the reaction rate, without being consumed during the reaction. Enzymes function as a catalyst in various biological processes taking place in all life forms. Although enzyme synthesis takes place inside living cells, they can even function as a catalyst in the in vitro processes, which makes it suitable for many industrial applications. For product formation in any chemical reaction, reactants or substrates has to overcome the transition state.

Starch is the most abundant polysaccharide carbohydrate reserve which is available in different parts of plants such as roots, stem, leaves, seeds, flowers, and fruits. It is considered an excellent source of energy and plants utilizes carbon from it for their metabolic activities (Smith, 2001). It is composed of glucose units linked together by glycosidic bonds to form amylose and amylopectin polymeric chains (Abbas et al., 2010).

The term nanoscience defines controlling or modifying the shapes and sizes of the materials such that they lie in the range of a nanometre (1 nm = 10-9 m) scale, typically 1-100 nm (Chavada et al., 2016). The application of these materials and systems by their suitable designing and production is what is known as nanotechnology. Richard Feynman, in 1959, first discussed the possibility of this advanced technology. However, the term was first used by Norio Taniguchi in 1974 (Chavada et al., 2016).

The exploration of facts about healthy and nutritious food has increased over the years. The advent of advance scientific tools has elucidated facts regarding the role of diet or nutrients in regulating gene expression (Sales et al., 2014). Various studies based on molecular interactions in food has revealed that many dietary components such as proteins, carbohydrates, fats, minerals, vitamins and phyto-constituents has the tendency to regulate gene expression.

Milk, commonly referred to as the “ideal food,” is an essential source of nutrition for human consumption. Because of the growing human population and the rising demand for milk and milk products, there is more interest in and concern about the quality and safety of dairy products. Milk comprises chemical substances with various functions in the human body, such as carbohydrates, lipids, proteins, and trace elements (vitamins, minerals, etc.) (Guetouache et al., 2014).