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This volume is the compilation of twenty-six research and review articles contributed by senior faculty members of different universities and scientific institutions. With its application oriented and interdisciplinary approach, we hope that the students, teachers, researchers, scientists, policy makers and environmental lawyers will find this volume much more useful. Editors are grateful to the following under mentioned distinguished scientists and other fellow colleagues for their constant encouragements, suggestions, valuable guidelines and necessary help. These distinguished, beloved scientists and well-wishers are Dr. Anurag Kr. Singh of IMS-BHU, Varanasi (Uttar Pradesh), Dr. Anurag Protim Das of Gargaon College, Simaluguri (Assam), Dr.S. R. Sitre and Dr.A. K. Pimpalshende of Nilkanthrao Shinde Science and Arts College, Bhadrawati (Maharashtra), Dr. S. M. Hussain and Dr. B.N.Hazarika of College of Horticulture and Forestry, Pasighat (Arunchal Pradesh), Dr. T. H. Dave, Dr.Hardik Sikotariya, Dr. Ketan V. Tank, Dr.Ketan Makwana and Dr. Pinak Bamaniya, Dr. H. L. Parmar, Dr.Sayan Biswas, Dr.Neha Kharadi and Dr. Mayur Bhadarka, Dr.Mohit Bamaniya and Dr. D. T. Vaghela of Kamdhenu University, Veraval (Gujarat), Dr. Devashish Ka of Department of Life Science and Bioinformatics, Silchar (Assam), Dr. Gora Shiva Prasad of West Bengal University of Animal and Fishery Sciences, Kolkata (West Bengal), Dr.H. Puinyabati of Pravabati College, Mayang Imphal (Manipur), Dr.Tribeni Hazarika, Dr. Januka Sharma, Dr. Monjeet Sonowal of Arunachal University of Studies, Namsai (Arunachal Pradesh), Dr.Kardivel Govindasamy, ICAR-ATARI, Guwahati (Assam), Dr. H.S. Praveenjoshi, Dr. Hariyala Sirisha and Dr. Kranthi Kumar Dhande, Andhra Pradesh Fisheries University, Narasapuram (Andhra Pradesh), Dr. M. Shomorendra of Thambal Marik College, Oinam (Manipur), Dr. M. Sivagangai of Periyar Arts College, Devanampattinam, Cuddalore (Tamil Nadu), Dr. Mostafa A. M. Soliman and Dr. Safwat Gobran and Dr. Mohammed A. A. Abo-Taleb of Agricultural Research Centre, Giza (Egypt), Dr. Pravin U. Meshram of Sevadal Mahila Mahavidyalaya, Nagpur (Maharashtra), Dr. R. Anandhan and V. Kavitha of Government Arts College, Kumbakonam (Tamil Nadu), Dr. S. Borthakur of College of Fisheries, Raha, Nagaon, Dr. Sanyogta Meshram of Shree Ramdeobaba Engineering College, Nagpur (Maharashtra), Dr. Sanyogita R. Verma and Dr. Vaishali A. Meshram of Anand Niketan College, Anandwan, Warora (Maharashtra), Dr.Sharda Dhase and Shahnoor Khan of CSIR-National Environmental Engineering Research Institute, Nagpur (Maharashtra), Dr. Shreya D. Indurkar, Seevadal Mahila Mahavidyalaya, Nagpur (Maharashtra), Dr. Vaishali P. Meshram, M.P. Deo Dharampeth College, Nagpur (Maharashtra), Dr. Aasidhara Darvekar, K.Z.S. Science College, Bramhani-Kalmeshwar (Maharashtra), Dr. Shyama Prasad Biswas of Dibrugarh University, Dibrugarh (Assam), Dr. Budhin Gogoi, Moran College, Moranhat, Charaideo (Assam), Dr.Vivekanand Safi and Dr.Tadang Meena of KVK, Karsingsa (Arunachal Pradesh), Dr. Tilling Tayo, KVK Longding, ICAR for NEH Region (Arunachal Pradesh), Dr. Shivendra Kumar, College of Fisheries, Dholi (Bihar), Dr. Ram Kumar, Koshi College, Khagaria (Bihar), Dr. Parmanand Prabhakar, College of Fisheries, Kishanganj (Bihar), Dr. Mukesh Kumar Singh, College of Fisheries, Dholi (Bihar), Dr. Nabam Gama, KVK, Karsingsa, (Arunachal Pradesh), and Dr. T. Neeraja, Andhra Pradesh Fisheries University, Narasapuram (Andhra Pradesh).

INTRODUCTION Ecosystem services and biodiversity conservation are well recognized as essential to human well-being and have become a policy priority. India is gifted with a large coastline and vast stretches of coastal wetlands. The estuaries and back waters which may include mangroves, mud flats, bays etc. extend over a large part of the Indian coasts. The mangrove ecosystem functions as a habitat for nurseries, thus benefiting fishers in the food chain (Mumby et al., 2004; Venkatachalam et al., 2018). Mangrove habitats increase the survival ratio of young fish that migrate to other offshore habitats when they mature, resulting in the enrichment of fish biomass in adjacent mangrove areas (Mumby et al., 2004; Aburto-Oropeza et al., 2008). Mangroves enhance fish production by providing food and shelter to fishery resources. Mangrove forests are highly productive, with average primary productivity level similar to that of tropical terrestrial forests (Alongi, 2009). Therefore, mangrove loss could have a devastating effect on fishery outcomes. The estimated fish catch is up to 70% higher in mangrove areas than in non-mangrove areas (Das, 2017). NATURE OF MANGROVE FORESTS Mangroves are amongst the most productive marine ecosystems on the Earth, providing a unique habitat opportunity for many species and key goods and significant services for human beings. They are salt tolerant plants, and are found distributed growing in estuaries, lagoons, backwaters and bays at the land-sea interface in tropical and subtropical areas of the world. Mangroves thrive on low-oxygen through the breathing roots or pneumatophores that are used for respiration. They need freshwater for their growth and so they live at the sea edge, they must remove the salts from the water that is taken in by the roots.

INTRODUCTION Biodiversity can be useful as an ecosystem indicator for conservation and monitoring, through continuous assessment of its main properties including stability, primary productivity, exploitation tolerance and even global environmental changes (Douglas et al., 2012). Crustaceans are found one of the most morphologically diverse taxonomic groups on the planet (Martin and Davis, 2001). Among them, decapods are the most studied taxon, mainly on account of the commercial interest of some species and their great diversity (Isarch and Munoz, 2015). Decapods are chiefly composed of marine species that live in waters depths ranging from shallow to deeper than 5000 m. Isarch and Munoz (2015) described the importance of this group lies in several factors such as the great biomass they represent, their significant role in marine food webs and the commercial interest of many decapod species. Order Decapoda comprises the commercially important shrimps, crabs and lobsters. Out of 649 species of marine crabs so far recorded from Indian waters, only 12 species of edible crabs namely Portunus sanguinolentus, P. plagicus, Charybdis feriatus, C. lucifera, C. annulata, C. natator, Scylla serrata, S. tranquebarica, Matuta lunaris, Sesarma tetragonum and Varuna litterata, which inhabit the coastal waters and adjoining brackishwater environments, support commercial fisheries (Menon and Pillai, 1996). However substantial quantities of crabs are landed every year as by-catches of shrimp trawlers and indigenous fishing units throughout the county. India represents one of the major contributors to the world production of marine crustaceans. Shrimps constitute as the main seafood export industry for the major foreign exchange earner as well as a source of livelihood for millions of fish workers. As shrimps are targeted for fishing, information on the present status of the fishery is critical for implementation of proper management. Some of the important penaeid shrimps that support commercial fisheries along the Indian seas are Fenneropenaeus indicus, P.semisulcatus P.monodon, P.merguiensis, P.japonicus, P.penicillatus, Metapenaeus dobsoni, M.monoceros, M. affinis, M. kutchensis , M. brevicornis, Parapenaeopsis stylifera, P.hardwickii, P.sculptilis, P.maxillipedo, P.uncta, Trachypenaeus curvirostris, Metapenaeopsis stridulans, Parapenaeus longipes, Solenocera crassicornis and S.choprai (Mohan Joseph and Jayaprakash, 2003). Rajkumar et al. (2015) carried out molecular identification of shrimp species, Penaeus semisulcatus, Metapenaeus dobsoni, Metapenaeus brevicornis, Fenneropenaeus indicus, Parapenaeopsis stylifera and Solenocera crassicornis from Tamil Nadu, India.

INTRODUCTION Species of the Clupeiformes (Teleostei), commonly known as herrings, shads, menhadens, sardines, anchovies, and their relatives, are a major component of forage fishes in coastal ecosystems and dominate worldwide forage fish landings (Tacon and Metian, 2009a; FAO, 2020). Clupeiformes are globally distributed fishes with having nearly 419 marine, freshwater, and diadromous species (Bloom and Egan, 2018) and they are filter-feeding fishes that form large schools having a diverse group of trophic guilds and habitats (Bloom and Egan, 2018). Additional to providing ecological and economic support, clupeiforms contribute to global food security given their abundance, easy access, and exceptionally high nutrient content (FAO, 2018). In some human communities, clupeiforms comprise the major or the sole protein source (Alder et al., 2008; Mohanty et al., 2019). Historically, clupeiform presence has been associated with persistent human settlement, growth, and survival (Thornton et al., 2010; Levin et al., 2016). Clupeiform fishes are ecologically diverse and span all aquatic habitats, including coastal and open marine environments, oceanic islands, estuaries, and freshwater rivers and lakes (Whitehead, 1985; Lavoue et al., 2013). Species can be restricted to marine, estuarine, or fresh waters, or they can be euryhaline, where a subset exhibit diadromy (Whitehead, 1985). Strictly marine clupeiforms (33.7% of all species) are distributed in every ocean, except for the Southern Ocean (Whitehead, 1985), while strictly freshwater species (17.8% of all species) are found on every continent except for Antarctica (Bloom and Egan, 2018). Major threats to clupeiforms are similar to those found for other groups of fishes (e.g., Roberts and Hawkins, 1999; Reynolds et al., 2005), with overexploitation as the leading threat for all clupeiforms in all habitats. While overexploitation may be the most prolific threat by impacting the highest number of clupeiforms, pollution may be the most detrimental, as it affects greater numbers of CR species. When compared to other economically and ecologically important fish groups globally assessed using the IUCN Red List methodology, clupeiforms have the lowest estimated percentage of threatened and NT species overall. Using the midpoint of species evaluated as elevated concern, roughly 11% are currently at high risk compared to approximately 22% of tunas and billfishes (Collette et al., 2011), 19% of sparids (Comeros Raynal et al., 2016), and 19% of groupers (Sadovy de Mitcheson et al., 2020).

INTRODUCTION Coral reefs are fragile and most diverse and valuable ecosystems on earth. The stony corals are formed from the tiny, soft colonial organisms called polyps. They provide enormous ecological, economic services, and cultural value to hundreds of millions of people. They are significantly contributing to the nutrition, economic security and protection from natural disasters. They are often termed as the rain forests of the sea. Coral reefs were first appeared 485 million years ago during the early Ordovician period. They belong to the class Anthozoa of animal phylum Cnidaria, which includes sea anemones and jelly fish. Coral reef is an under water ecosystem which is characterized by reef-building corals. Unlike sea anemones, colonies of coral polyps secrete a hard exoskeletons called reef which is made up of calcium carbonate to support and protect the corals. These reefs grow best in warm, shallow, clear, sunny and agitated water. BIODIVERSITY The term biodiversity was coined by Walter G. Rosen in the year 1985. Biodiversity refers to the variation among living organisms that can be found in a particular area such as terrestrial, marine and desert ecosystems and the ecological complexes to which they are a part. The study of biodiversity helps to understand the ecological stability, economic and ethical importance. Coral reefs are believed to have the highest biodiversity on the planet even more than a tropical rainforest. Though the coral reefs distributed in less than one percent of the ocean floor, they provide habitat for more than 25% marine life with more than 4,000 world’s fishes. They act as protective nurseries to many fish species, and shelter small fishes to grow. Coral reefs diversity is so rich that we do not have a firm count on all the species that live within it and every year discover new species.” There are about six thousand coral species in the world, in which few are growing in warm shallow waters near coastlines while others in the dark, cold seafloor of the open ocean.

Increasing pressures exerted on the environment by humans make protection of natural areas crucial for the preservation of biological diversity. Protected areas are one of the most effective tools available for conservation of biodiversity. The IUCN defines a Protected Area as ‘an area of land and/or sea specially dedicated to the protection and maintenance of biological diversity, and of natural and associated cultured resources, and managed through legal or other effective means. Since 1986, the IUCN commission on National Parks and Protected Areas (CNPPA) has been promoting the establishment and management of a global representative system of Marine Protected Areas (MPAs) which play a critical role in the conservation of biodiversity. MPAs are growing in importance globally as practical and potentially effective options for the management of fisheries, the protection of biodiversity and the generation of income from eco-tourism. The effective management of MPAs to ensure that they meet their declared objectives poses many challenges. The IUCN has defined an Marine Protected Area (MPA) as ‘Any area of the intertidal or subtidal terrain, together with its overlying water and associated flora, fauna, historical and cultural features, which has been reserved by law or other effective means to protect part or all the enclosed environment (Kelleher,1999).

Study done in India on various aspects of marine copepods are those of Krishnaswamy (1952), Kartha (1959), Ummerkutty (1960, 1966), Ganapathi et al. (1961), Kasturirangan (1963), Saraswathy (1966), Abraham (1970), Silas and Pillai (1973), Sarladevi (1979), Sarkar et al. (1985), Madhupratap and Haridas (1986), Wells and Rao (1987), Gajbhiye et al. (1991), Madhupratap (1999), Kesarkar and Anil (2010), Ramanibai and Shanthi (2011), Srichandan et al. (2014). Jayabarathi et al. (2015), Nishida et al. (2015), Arunpandi et al. (2017), Loka et al. (2017), Deepika et al. (2019), Kaviyarasan et al. (2019), Khandagale et al. (2022), Nawaz et al. (2023), Thangaraj and Vijayanand (2023). Copepods are planktonic crustaceans, which occur in marine, fresh as well as brackish waters. They represent about 80% of the zooplankton in the ocean. There are more than 210 families, 2400 genera and 24000 species identified in this group. Planktonic copepods are considered to be the most abundant metazoans on earth. The name 'copepod' is derived from the Greek words meaning 'animals with oar shaped foot' i.e., ‘kope’ means oar and ‘podos’ means foot (Stottrup,2003). Copepods are important secondary producers and primary consumers and ultimately contribute significantly to the food chain in large ecosystems. Copepods are very hardly planktonic forms which can withstand most of the unfavourable conditions and can produce diapause eggs and resting eggs to survive in these conditions. Most of the copepods can adjust wide range of salinity and temperature levels. Due to its hardy nature, copepods can be easily introduced into all types of water bodies. Copepods forms important food for many aquatic animals especially fishes. Certain fishes and fish larvae were evolutionary adapted to feed on copepods. Copepods have been found to be nutritionally superior to almost all other live feeds.

INTRODUCTION The Food and Agriculture Organization (FAO) of the United Nations defines aquaculture as the cultivation of aquatic organisms such as fish, mollusks, crustaceans, and aquatic plants by individuals or corporations. In aquaculture, interventions are made in the rearing process to enhance production. While aquaculture is a practice with origins dating back more than 3000 years, Aquaculture products have only recently been recognized by the United Nations Statistical Commission as distinct global commodities, despite the practice having origins dating back more than 3000 years. According to the FAO's "Global Aquaculture Production" database, the annual global production of aquaculture has increased at an average rate of around 8% over the past three decades (since 1980). Despite a recent slowing in global trends, growth remains higher than any other major animal food production sector. This rapid global increase in production is occurring alongside a slow decline in marine fisheries catches. There is a growing global focus, largely driven by demand from Western countries, on increasing the intensive production of omnivorous and carnivorous species farmed and harvested in maritime and brackish coastal environments, a practice known as mariculture. The farming of these species, such as salmon, groupers, seabasses, and prawns, typically relies heavily on inputs such as wild post-larvae and juveniles, fishmeal, fish oils, as well as significant amounts of water, land, and energy. As a result, mariculture has faced widespread criticism due to its often negative impacts on marine and coastal ecosystem health. Negative associations have been drawn between mariculture and its effects on marine capture fisheries and food security.

INTRODUCTION The aquaculture industry is regarded as one of the most advanced food production industries globally. From 2010 to 2020, it grew at an average annual rate of 4.6%, reaching 122.6 million tonnes of live weight (FAO, 2022). Sustainable aquaculture proposes numerous profits to attain economic progress through well usage of natural resources (FAO 2017). The expansions in the sustainable aquaculture business are challenging due to the inadequate number of available natural resources and industrialized effects that released to the surround environments (Costa-Pierce et al. 2012; Verdegem 2013). Particularly in intensive system, increased higher organic matter concentrations raise several future issues, including acute harmful, chronic impacts (Piedrahita 2003). Recently, Innovative biofloc technology system (BFT) is one of the most popular methods to combat these problems. Biofloc is a climate-smart technology that relies on mass synthesis of in situ microorganisms (Ogello et al., 2021). The BFT permits high stocking densities and higher fish productivity (Ogello et al., 2021) while requiring limited or zero water exchange in to sustain the flocs (Emerenciano et al., 2017). The power of BFT aligns with the "cradle to cradle" concept that outlined by McDonough and Braungart (2002), which omits the phrase "waste”. In BFT, nitrogen wastes that generated from feces of aquatic animals and residual feeds are transformed into the microbial protein that becomes feed for the same creatures (Minaz and Kubilay 2021). Numerous microorganisms, including bacteria, microalgae, protozoa, and zooplankton, are present in biofloc particles (Ray et al. 2010a). Hence, biofloc can incorporate the diet of aquatic species, which utilises the natural productivity in the culture system as a feed source (Wasielesky et al. 2006; Emerenciano et al. 2012a). This microorganisms are recognised for their contributions to (i) preserving high water quality (Emerenciano et al., 2017), (ii) enhancing culture efficiency by lowering feed conversion ratio (FCR) and costs of feed (Hargreaves, 2006). (iii) biosecurity (Defoirdt et al., 2004), and (iv) conserving greenhouse gases (GHG) (Manan et al., 2016). These four biological roles of microorganisms in BFT units are factors of high fish production, profitability and environmental protection (Ogello et al., 2021).

INTRODUCTION Aquaculture is a technology driven industry which relies a lot on research to develop species diversification in culture system as well as in the appropriate technology for commercial production. The rapid development of aquaculture in the world depends largely on the availability of fish feed; which are traditionally based on fish meal as the main protein source (Hardy & Tacon, 2002; Krishnankutty, 2005; Yigit et al., 2006) but the major challenge is its availability, quality and cost (Ghazala et al., 2011; Tabinda and Butt, 2012). In the aqua feed formulation, protein is the main but expensive ingredient and its quality and quantity in fish feeds formulation plays a vital role in promoting fish growth (Pandian, 2001). Dietary protein provides essential and non essential amino acids to synthesize body protein and energy for maintenance. According to fish species, fish size, dietary protein sources and environmental conditions protein requirements ranges from 300 to 550 g /kg (NRC 1993). In India there is a significant development in fresh water aquaculture in last few decades contributing greatly to overall fish production of the country. The fresh water aquaculture is crossing the traditional boundary of concentration on farming of major carp and entered the arena of diversification to the culture of other species like catfishes. So far in India majority of research works were concentrated on development of commercial fish feed mainly for carps and shrimps. To fulfill the objectives of large scale culture of cat fishes and development as an economic proposition, it is necessary to take up induced breeding and larval rearing of commercially important catfishes in controlled conditions and develop easily adoptable package of practices of the same to cater to the needs of interested fish entrepreneurs. Among the catfishes, Clarias magur, an air breathing species and popularly known as ‘magur’ or ‘walking catfish’ is a high valued fish in the Indian subcontinent and fetches higher price than many other fishes including major carps and it is highly revered for its nutritious flesh which is therapeutic in nature, hence, better prospects are there for developing its culture (Hussain et al., 2016). Vishwanath (2010) reported that this species is very much popular in Bangladesh due to use as important part of the diet for children and lactating mothers and also prescribed as diet for the convalescent of the patients. This fish is highly regarded for food due to its high protein (15.0%), low fat (1.0%) and high iron content (710mg/100g tissue). Paul et al. (2015) in their studies on Indian catfish namely magur (Clarias batrachus) and singhi (Heteropneustes fossilis) for proximate composition and mineral content concluded that both the fishes are rich in protein, fat and minerals content irrespective of their size and season.

INTRODUCTION An expanding aquaculture industry that offers high incomes and job opportunities to people worldwide is ornamental fish farming. With over 2 billion live ornamental fish traded globally, the ornamental fish trade has become a very lucrative sector (Das & Biswas, 2016; Katia, 2001) with global net worth of more than 20 billion USD. According to Saxena (1994), the most significant factor influencing an ornamental fish's market value is its vibrant body colouration. Fish are incapable to synthesize carotenoids De novo (Goodwin 1951),and rely on dietary carotenoids for its body pigmentation (Hata and Hata, 1973). So, there is hence a direct correlation between pigmentation and dietary carotenoids(Halten et al., 1995). Aquatic animals' skin, meat, shell, and exoskeleton are coloured red, orange, and yellow due to a class of naturally occurring lipid-soluble organic pigments called carotenoids (Pailanet al., 2012). Fish skin and muscle colour regulation is significantly influenced by dietary carotenoids (Ahilan et al., 2008). The freshwater ornamental fish industry has encountered the issue of fadingfish body colouration, particularly in cases where the fish are reared in captivity for extended periods of time and subjected to rigorous cultivation conditions (Das and Biswas, 2016). According to Das and Biswas (2016), an ornamental fish's food should generally be nutritionally balanced, appetizing, resistant to crumbling, water stable, buoyant, and enhance or retain the fish coloration. The phenomena of coloration in fish are quite significant and have a direct impact on its aesthetic value. If pigment-enriched feed can be prepared and used to increase coloration, the fish will undoubtedly have better quality and a cheaper price tag. Detailed research on colour enrichment in native ornamental fish using formulated feed is lacking (Das and Biswas, 2016). For the purpose of colour enrichment in fish, native plant sources have been used. Choubert (1979), Boonyaratpalin and Phromkunthong (1986), and Alagappan et al. (2004), utilized spirulina as a source of carotenoid colours for fancy carp and rainbow trout. Enhancement of pigmentation of Cyprinus carpio and Carassius auratus with microalgal biomass supplementation has demonstrated that Chlorella vulgaris is just as effective as synthetic colours (Gouveia et al., 2003). Alagappan et al. (2004) observed increased body pigmentation in Trichogaster trichopterus using Spirulina sp. algae as a source of carotenoid pigment. The body pigmentation of goldfish (Carassius auratus L.), red swordtail (Xiphophorus helleri), and tiger barb (Puntius tetrazona) was enhanced by feeding with marigold petal diet (Boonyarapatin and Lovell, 1977; Ezhil et al., 2008; Alma et al., 2013). Using natural sources of carotenoid pigments to improve the colour of ornamental fish is a cost-effective and ecofriendly solution. Due to the aforementioned information, the current study was carried out to assess how dietary natural carotenoids from source such broccoli (Brassica oleracea var. italica) effects the development and coloration of Blue Gourami, Trichogaster trichopterus, one of the important ornamental fishes with high market value.

INTRODUCTION Fish live in a variety of watery habitats and comprise around 50% of all vertebrate species worldwide. There are approximately 21,723 known living fish species out of a total of 39,900 vertebrate species, with 8,411 found in freshwater habitats and 11,650 in marine environments (Jayaram, 1999). Fish are used as markers of contamination and contribute significantly to the preservation of human history and welfare, either directly or indirectly. According to Mittermeier et al. (1997), India ranks eighth in the world for freshwater biodiversity, making it one of the nations with significant biodiversity. There are over 2,500 fish species in India; roughly 930 of them are found in freshwater environments, and roughly 1570 are found in marine ones (Kar et al., 2006). Fish diversity is particularly rich in the northeastern part of the nation; studies have shown that there are 267 freshwater fish species from 114 genera distributed over 38 families and 10 orders (Mahanta et al., 2003). Fish plays a crucial role in Assam's cuisine, where a wide range of fish species may be found in the region's floodplain wetlands because of the subtropical climate, favorable ecological and topographical circumstances, and inherent capacity for stocking. Assam's waters, including its wetlands, are home to around 217 fish species, representing 104 genera, 37 families, and 10 orders, according to Goswami & Singha (2023). Assam's lakes and marshes are vital for supplying the state's domestic fish needs, fostering economic growth, and giving the general public access to fairly priced food. In Maguri Beel, several fish are in danger of going extinct because of pollution, habitat damage, and the introduction of non-native species, overexploitation and human activity (Moyle & Moyle, 1995; Malakar & Boruah, 2017). The 2020 oil spill incident might have had a major impact on the variety of the ichthyofauna's composition. To address the same, a comparison of the same with earlier research is desperately needed. As a result, the current study intends to examine the fish variety of Maguri Motapung Beel, close to Motapung Village in the Tinsukia region of Assam and Dibru-Saikhowa National Park.

INTRODUCTION The aquatic ecosystem is highly dependent on water quality and biodiversity. The water quality parameters play an important role in the biology and physiology of fish (Dhawan and Kaur, 2002). Fish communities are an important aspect of the aquatic ecosystem. Moreover, they also serve as an indicator of the quality of the aquatic ecosystem (Sharma et al., 2014). Ramsar Convention on wetlands in 1971, characterized wetlands as "Areas of Marsh, fen, peatland, whether natural or artificial, permanent or temporary with water that is static or flowing, brackish or salt, including areas of marine water the depth of which at low tide should not exceed". At the surface, freshwater serves as a habitat for numerous species and comprises a substantial portion of Earth's biological diversity. Wetlands cover approximately 6.4% of the Earth's total surface area. Being a large biodiverse region, India accounts for 11.72% of the world's fish diversity and 40% of fish live in freshwater ecosystems (Kar et al., 2006, Balasubramanium, 2017). Fish are key species that determine the distribution and abundance of other organisms and have a significant impact on the ecosystem and are considered good indicators of water quality in the ecosystem (Moyle and Leidy, 1992). Assam, located in the northeastern region, possesses extensive and diverse freshwater resources, earning it recognition as one of the richest biodiversity sites in India. There are 2,500 species of fish in India; 930 of them live in fresh water and 1570 in the sea (Kar, 2003). The entire northeastern region, including Assam, has large and diverse freshwater resources and is considered one of the richest biodiversity hotspots of India. The NE region has unique topographic conditions and is blessed with large and varied water resources in the form of rivers (19,150 km); swimming pools (23,792ha); lakes and marshes (143,740 ha); ponds and mini-dams (40,808 ha) and shallow spawning systems paddy with fish farming systems (2780 ha) (Mahanta et al., 2003). This region of the country is very diverse in terms of fish. So far, 267 freshwater fish species belonging to 114 genera 38 families and 10 orders have been reported from the region (Mahanta et al., 2003). A total of 266 species belonging to 114 genera under 38 genera and 10 orders have been reported from the Northeast including Assam (Sen, 2010). A survey of the fish fauna of the Brahmaputra in Assam revealed 41 commercially important species (Talwar and Jhingran, 1991). Assam has 1,392 beels in over 100,000 hectares, which constitutes 61 percent of the state's lentic water supply. Water is one of the most important compounds in the ecosystem. Better water quality is characterized by its physical, chemical and biological characteristics.

INTRODUCTION The genus Pallisentis was erected by Van Cleave, 1928 with P. umbellatus as its type species. The other species reported under this genus are P. gaoes (Mac Callum, 1918) Van Cleave, 1928; P. cleatus Van Cleave, 1928; P. ophiocephali (Thapar, 1930) Baylis, 1933; P. nagpurensis (Bhalerao, 1931) Baylis, 1933; P. nandai Sarkar, 1953; P. colisai Sarkar, 1956; P. basiri Farooqi, 1958; P. allahabadi Agarwal, 1958; P. buckleyi Tadross, 1966; P. pandei Rai, 1967; P. guntei Sahay et al., 1967; P. garuai n. comb. Davendrosentis garuai Sahay et al., 1971; P. magnum (Saeed & Bilqees, 1971); P. tetradontae Troncy, 1978; P. clupei Gupta and Gupta, 1979; P. cavasi, P. fasciati, P. gomtii Gupta and Verma, 1980; P. indica, P. croftoni Mithal and Lal, 1981; P. kalrai Khan and Bilqees, 1985; P. guptai, P. mehrai Gupta and Fatma, 1985; P. fotedari Gupta and Sinha, 1991 and P. jagani Koul et al., 1991. MATERIALS AND METHODS The fish hosts were collected from the study area and brought to the laboratory for parasitic examination. The external body surface as well as the internal body organs were thoroughly examined for the parasites. The collected acanthocephalan parasites were washed and relaxed in normal saline. Then the specimen was fixed and preserved in AFA (alcohol-formalin-acetic acid) solution. To facilitate identification of the worms the parasites were cleared in lactophenol and mounted in glycerine jelly. Diagrams were made with the help of camera lucida and measurements were taken with ocular micrometer. All the measurements are in millimetres. The collected parasites were identified following Yamaguti (1963) and Bhattacharya (2007).

INTRODUCTION Lakes are of immense ecological, economic, and cultural significance, representing integral features within our planet's landscapes. Studies show that there are around 117 million lakes worldwide, constituting about 4% of the Earth's non-glaciated land surface (Verpoorter et al., 2014). These lakes play a crucial role in providing essential services to nearby communities and serve as important centres for recreation and overall well-being. However, the increasing demand for natural resources has led to widespread degradation of these lakes (Jenny et al., 2020). Despite efforts to restore lakes, there is a prevailing perception that such programs often encounter high failure rates (Søndergaard et al., 2007; Gulati et al., 2008; Poikane et al., 2024). This concern is particularly pronounced in less economically developed countries, where populations are highly exposed to the consequences of lake degradation, and monitoring, assessment, and restoration programs may be in nascent stages. Additionally, lakes play a pivotal role in climate regulation, influencing local weather patterns and contributing to global processes such as water and carbon cycles. Recognizing and preserving the significance of lakes is imperative for sustaining ecosystems, ensuring water security, and nurturing the well-being of both the natural environment and human societies. 21o 8’ 55’’ 79o 4’46’’E. Nagpur situated at coordinates 21o 8’ 55’’ N and 79o 4’46’’E, holds the distinction of being the second capital of Maharashtra. Renowned as the "orange city" and the "city of lakes," Nagpur relies significantly on its lakes as a valuable resource base. The current water supply for the city is primarily sourced from, Kanhan River, Gorewada Lake, and Pench Dam. Futala Lake serves an irrigation purpose, benefitting 84 acres of cultivated agricultural land. Ambazari Lake, on the other hand, caters to the water needs of local residents in the MIDC colony, Hingna area, Nagpur, and supports industrial purposes. Other notable water bodies in Nagpur include Sonegaon, Gandhisagar, Naik, Sakkardara, and Lendi. However, these lakes face severe pollution due to anthropogenic activities such as bathing, washing clothes, and improperly disposing of plastic, paper, oil, and worship materials. Moreover, the lakes Gandhisagar and Lendi are now dried.

INTRODUCTION Heterotrophic organisms, such as zooplankton, use phytoplankton as food, regenerating nutrients through metabolic processes and transferring energy to higher trophic levels. Zooplankton are involved in the conversion of waste matter into animal food that may be consumed, in addition to helping to shift food from the primary to the secondary level. These species act as a bridge in the food chain, carrying energy from the primary producers, planktonic algae, to the larger predatory invertebrates and fish that eat them (Kumari et al., 2008). Macróplankton (200–2000 μm), microplankton (20–200 μm), nanoplankton (2–20 μm), picoplankton (0.2-2 μm), and femtoplankton (0.02 0.2 μm) are the different sizes of plankton (Kumar et al., 2023). The sensitivity of zooplankton to alterations in aquatic environments is significant. Differences in species composition, abundance, and distribution of body size can be used to identify the consequences of environmental disturbances(Joshi & Joshi, 2011). A number of variables, including temperature, sunlight, ocean currents, and nutrition availability, affect plankton populations. They are vital to ecosystems because they support carbon cycling, global biodiversity, and the general health of the oceans (Cottenie et al., 2001). An evaluation of the pollution state heavily depends on the interaction between phytoplankton diversity and environmental conditions. The diversity of algae found in the Chlorophyceae family is a key sign of the quality of the water. The differences in water quality can be attributed to a combination of natural and human-caused factors. Owing to extensive human activity, anthropogenic inputs from various sources are typically the main variables influencing the water quality of the majority of rivers, lakes, estuaries, and seas, particularly those that are next to densely populated areas(Jeyaraj et al., 2016). Living things, including plants, plankton, animals, and bacteria, are known as bioindicators, and they are used to monitor the state of the environment's natural ecosystem. They are known across the world as aquatic pollution indicator species. Every organic entity in a biological system gives information about the state of its surroundings. For example, plankton responds quickly to changes in the environment and is a valuable biomarker for determining the quality of water as well as a sign of pollution. Plankton is the best indicator of the health of aquatic flora and serves as an early warning system(Wadjikar et al., 2017). An ecosystem that is in balance is one in which beneficial interactions occur between the environment and living organisms. Since water quality is essential to maintaining an environment in balance, it is evident that it plays a crucial role in this relationship(Jeyaraj et al., 2016).

INTRODUCTION In nature water is considered as the most important natural resource which is utilized in large quantity throughout the world for drinking, washing, bathing, recreation, irrigation, aquaculture and many industries. Water also plays a fundamental role in regulation of water cycle in nature. India is a agriculture based country totally based on different water sources. The quality of water plays a important role to mankind because it is directly linked with human health. Lake water’s are mainly used for farming and domestic activities like cloth washing. Increasing human activities continuously in lake basins is posing a great threat to all forms of life in aquatic environment. Most of the people who live in villages use irrigation facilities for farms and use excessive use fertilizers and pesticides for agriculture purposes and subsequently and degrade the aquatic ecosystems of lakes. We need to conserve water for future generations and safeguard our natural resources for sustainable development and biodiversity conservation. Many researchers investigated the freshwater lakes and ponds in the Indian subcontinent viz. Bagde and Verma (1985), Desai (2014), Patel and Patel (2012), Nirbhavanae and Khobragade (2017), Mehta et al (2016), Gupta (2018), Bastola (2013), Raj and Sevankodiyane (2018), Rana Phul Kunwar Singh (2016), Shyamala and Hemvathy (2018), Meshram et al (2014), Fule and Nimgare (2018), Chaudhary and Sitre (2020), Pimpalshende and Sitre (2021).

INTRODUCTION India is rich in biodiversity and possesses about 108276 species of insects. Indian subcontinent is one of the mega biodiversity countries of the world occupying ninth position in terms of freshwater mega biodiversity (Mittermeier et al., 1997) comprising 30 order (Dijkstra et al. 2013). Inland wetlands of India serve as the habitat for more than 500 species of aquatic insects which are represented predominantly by Ephemeroptera (mayflies), Odonata (dragonflies) and Trichoptera (caddisflies), (Subramanian and Sivaramakrishnan, 2007). Aquatic insects play important ecological roles in keeping freshwater ecosystems functioning properly (Choudhary and Janak, 2015). The aquatic insects are important part of aquatic ecosystem which play vital role in ecosystem functioning and are known to be representative of structure and function of freshwater ecosystem due to their high abundance, high birthrate with short generation time, large and rapid colonization (Choudhary and Ahi 2015). Aquatic insect function on multiple trophic level such as shredders, collectors, scraper and predators on animal debris, wood and leaf litter reaching the wetland from the surrounding landscape. Nutrients processed by aquatic insects are further degraded into absorbable form by fungal and bacterial action. Plants in the riparian zone absorb this nutrient soup transported through the wetlands. In addition to this significant ecosystem function, aquatic insects are also a primary source of food for fishes and amphibians (Tachet et al., 2003 ; Kumar, 2014).They interact in nutrient cycling within lentic and lotic systems and are regarded as an effective bioindicator (Lundquist and Zhu 2018) and serve as an important component of natural foodweb in aquatic ecosystem. However, the aquatic insect fauna of this part in India is inadequately documented. Therefore, present study carried out to delineate the baseline status of aquatic insects of Anandwan lakes at Warora Dist: Chandrapur.

INTRODUCTION Wheeler bearers commonly referred to as ‘Rotifers ‘are significantly they are often taken as reliable indicators of mesotrophic characteristics in the lake ecosystems (Gannon and Stemberger, 1978). Because of their wide tolerances to varied dissolved oxygen levels and temperature distributions, they are even found to inhabit the deeper waters of aquatic ecosystems (Esparcia et al., 1989). They belong to the Kingdom Animalia, Subkingdom Eumetazoa, Phylum Rotifera (Cuvier, 1798) having three classes Monogononta, Digononta and Seisonidea (Pennak 1953). In other words these wheel animalcules named, because of the presence of their corona, which is composed of several ciliated tufts around their mouth region. This ciliated tuft helps in locomotion and resembles a wheel. These create a current that sweeps food into the mouth, where it is chewed up by a characteristic pharynx (called the mastax) containing a tiny, calcified, jaw-like structure called the trophi. The cilia functions to pull the animal, when unattached, through the water. Most free-living forms have pair of posterior toes to anchor themselves while feeding. Rotifers are bilaterally symmetrical found in various shapes. There is a well-developed cuticle, thick and rigid, providing a box-like shape, or flexible, giving the animal a worm like shape; such rotifers are respectively called loricate and illoricate. Habitats of Rotifers are relatively easy to find. Many live in ponds, moist soil, or any stagnant water. Rotifers can be free swimming or sessile. Rotifers are mostly omnivorous and some have been observed to be cannibalistic. They normally eat algae or decomposing organic material. As they are a major food source for Cladoceran , Copepodans , juvenile fishes (fry) and fishes, therefore , an important food link of water ecosystem (Arora and Mehra 2003). Presence of abundant Rotifers in freshwaters determines them to be one of the main groups of zooplankton in limnological studies. They are permanently and obligatorily connected to aquatic habitats in all active stages and only their resting stages are drought-resistant (Chopra et al 2012). Various researchers have conducted pioneer study on the diversity and habitat of rotifers such as Anderson 1889; Sharma 1987; Sharma 1998a; Gilbert and Schreiber 1998; Arora and Mehra 2003; George et al. 2011; Tyor and Chawala 2012. However such studies on Madhuganga dam water has not been conducted, hence for assessment of Madhuganga Lentic water has been conducted for the seasonal variation in diversity and density of rotifers.

INTRODUCTION Indigenous Technical Knowledge (ITK) represents the treasure trove of wisdom held by indigenous communities, often overlooked yet immensely valuable. Its impact spans various domains, notably aquaculture, where it has been pivotal. Local fisher communities, drawing from their indigenous knowledge, have been crucial in safeguarding wild fish genetics and improving aquaculture yields. Moreover, their Indigenous and local knowledge (ILK) contributes multifaceted insights to fisheries and aquatic ecosystems science, underscoring the need to preserve and honor this invaluable heritage. Additionally, traditional indigenous systems like aub-anbars have historically demonstrated their effectiveness in practical applications such as passive cooling and water storage, further highlighting the relevance and efficacy of ITK. Importance of ITK in Aquaculture Indigenous Technical Knowledge (ITK) is essential in aquaculture, providing valuable methods for pond preparation, water quality management, disease control, and post-harvest operations. These traditional practices, backed by scientific validation, can greatly improve fish production sustainability while being environmentally friendly. Moreover, modern biotechnological tools are increasingly used in aquaculture to enhance fish quality, disease resistance, growth rates, and genetic diversity. Additionally, the concept of intelligent fish farms, employing technologies like IoT and artificial intelligence, aims to optimize aquaculture processes for precise feeding, disease control, and harvesting, promoting green and sustainable practices. Combining ITK with modern biotechnology and intelligent technologies is crucial for advancing aquaculture practices towards efficiency, sustainability, and increased productivity.

INTRODUCTION The expansion of aquaculture, a vital source of income for a large portion of the global population (Salam et al., 2014), has created an unforeseen challenge: increased competition for land use with traditional agriculture. This land squeeze is further intensified by a multitude of factors. Shrinking arable land due to urbanization and desertification, coupled with the relentless growth of the human population, puts immense strain on existing agricultural resources. Man-made environmental pollution from industrial waste and agricultural runoff, along with the far-reaching effects of climate change, add another layer of complexity. These combined pressures necessitate a paradigm shift towards innovative and sustainable agricultural practices. One promising solution gaining traction is aquaponics, a system designed to cultivate plants and aquatic animals together in a carefully balanced, recirculating environment (Salam et al., 2014). This approach leverages a symbiotic relationship between fish and plants. Fish waste, rich in nitrogenous compounds, acts as a natural fertilizer for the plants growing in the system. These plants, in turn, function as biofilters, removing excess nutrients and purifying the water for the fish. In essence, aquaponics integrates two established agricultural techniques: intensive aquaculture, the practice of raising fish in controlled tank environments, and hydroponics, the cultivation of plants without soil (FAO, 2012). The aquaculture component of aquaponics utilizes eco-friendly, indoor recirculating systems that enable meticulous control over water quality and temperature year-round. This controlled environment offers significant advantages over traditional methods, minimizing dependence on external factors like weather patterns.

INTRODUCTION The aquaculture sector expands and environmental regulations become stricter, waste management has emerged as a significant responsibility for managers of aquaculture farms. Waste generated by aquaculture facilities usually comes in the form of either effluent or sludge. Effective management and disposal of wastewater in aquaculture are becoming progressively crucial due to the strict regulations concerning the discharge of waste into natural water systems. The worldwide expansion of aquaculture industries has led to a rise in adverse environmental effects due to the release of significant quantities of polluting effluents containing uneaten feed and fecal matter (Chávez Crooker and Obreque-Contreras, 2010) Efficient management of wastewater in aquaculture is crucial for fostering sustainable practices. A variety of innovative methods have been developed to treat aquaculture wastewater effectively. These encompass eco-sensitive systems such as aquaponics and integrated multi-trophic systems, along with biofilm technologies that exploit the mutual growth of algae and fungi to remove nutrients. Furthermore, the reuse of wastewater via oxidation ponds can both provide natural sustenance for fish and treat household sewage effectively. Technologies like constructed wetlands and recirculation systems have proven highly effective in removing nutrients and recycling water, thus aiding in sustainable aquaculture practices. Additionally, specialized continuous automatic treatment equipment has been devised specifically for aquaculture wastewater, ensuring efficient purification processes. Together, these diverse strategies collectively strive to address the challenges posed by nutrient pollution and resource depletion in managing aquaculture wastewater.

INTRODUCTION Phenolic compounds in the wastewater are harmful pollutants which originate as byproducts from several industries like pulp and paper mills, coaking plants, petroleum refineries, tanning industries, phenolic resin manufacturing units etc. Phenolic compounds impart colour and odour to water and are toxic even at a very low level of concentration. The various processes for the removal of phenolics from the wastewater can be grouped into two broad categories. In one category are those processes like microbiological degradation, chemical treatment, incineration etc. which aim at destroying the phenolics, while the other category has processes involving the recovery of phenolics e.g. solvent extraction, activated carbon treatment, uptake by porous polymeric adsorbents etc. Among these various methods polymeric adsorbents are very promising for the removal of phenolic compounds due to following reasons. The efficiency of removal of phenolics by porous polymeric adsorbents and their subsequent elution from the polymer matrix is exceptionally good. The requirement equipment is very simple and the operation is usually trouble free. The operation can be carried out at ambient temperature and the space requirements are comparatively small. Very stringent specifications regarding the quality of the effluent can be met by the use of polymeric adsorbents.

The Cladocera, commonly known as ‘waterfleas’ form a primitive group of microcrustaceans. They play an important role in the aquatic food chain and also contribute significantly to the zooplankton dynamics and secondary productivity in freshwater ecosystem. Though most cladocera is filter feeders, they also feed on detritus, algae, small rotifers and copepod nauplii. They keep a check on eutrophication for which this group is also widely used as good indicators of ecosystem health and sentinels of environmental change (Eggermont and Martens, 2011; Padhye and Dahanukar 2015). At the same time, cladocera is the favorite food prey of many invertebrates and many vertebrates. Their characteristic jerky movements make them easily visible to the predators and that is why most of fish larvae were found to prefer cladocera over the prey (Mayer and Wahl,1971). Cladocerans have a distinct head, a single compound eye, and a large mandible for grinding food particles. Cladocerans feed on algae, small rotifers, and copepod nauplii. Cladocerans reproduce mostly asexually via parthenogenesis. But they can also reproduce sexually based on the environmental conditions. The distribution of cladocerans is affected by different factors including temperature, rainfall, water quality, nutrients, macrophytes, flood pulse etc. (Ghidini et al.,2009; Kiss et al., 2014). Their metabolic rate is variable with water temperature. The order cladocera belongs to the subclass Branchipoda and includes minute crustaceans generally in the size range of 0.2 to 5.0 mm.

INTRODUCTION Aquaculture is the fastest growing food producing sector with annual growth rate of 10.34% and it play a very important role in improving the income generation as well as nutritional security of farmers in many developing countries including India. In the fresh water system, carps are the most widely cultured species throughout the country and carp culture in India is believed to be as old as carp culture in China. Although in early years, carp culture was largely restricted to the North-Eastern States of India, during the last four to five decades, it has spread to almost all parts. The traditional carp culture practice is rapidly transforming into scientific farming in several parts of India. Generally, carp culture in India is a polyculture of 3-6 species of major carps and exotic carps (Vijay Anand, 2019), namely, catla (Catla catla), rohu (Labeo rohita), mrigal (Cirrhinus mrigala), silver carp (Hypophthalmichthys molitrix), grass carp (Ctenopharyngodon idella) and common carp (Cyprinus carpio). Among six species, common carp (Cyprinus carpio) cultured in the composite fish farming system is potential species, which plays a vital role in the Inland Fish production especially for the North Eastern Hill states of India as the species has got high adaptability and tolerance towards high range of temperature and dissolved oxygen variation. The species is introduced to India in 1937 as the Prussian strain and in 1957 as the Bangkok strain. Due to their high food value, the farmers of the state have been culturing the fish in rice fields in monoculture or along with other species particularly in Apatani Plateau of Ziro area gaining an average weight 75g to 110g (Hussain et al., 2018).

INTRODUCTION Integrated farming is an innovative approach towards increased fish production. In integrated fish farming two or more commodities are framed together on a common infra-structural base with the objective of optimizing use of available resources. It is based on the concept that “there are no wastes and waste is only a misplaced resource, which can become a valuable material for another product”. There are several advantages of integrated farming system viz. increased productivity, greater income, improved cash flow, fuller employment, a better diet for the farmer’s family and less biological and economic risk (Pillay, 1990). On the other side, there is the disadvantage that the integrated system is more complex, with a need for more knowledge and better management, since the failure of one system could adversely affect other system (Singh et al., 2014). In integrated farming system, animal manure is shed directly into a fish pond as fertilizer and supports the growth of natural fish food organisms. The fish not only utilize spilled animal feed, but also directly feed on fresh animal excreta which is partially digested and is rich in nutrients. Surplus excreta support the rich growth of planktonic fauna. Fertilizers and supplementary feed are not used, resulting in a drastic cost reduction. These farming systems are relatively confined units with little exchange of water. Manure from livestock which indirectly trigger the growth of mainly photosynthetic organisms resulting in the production high yields fish with low input and limit the dependency on supplementary feed (Saikia et al., 2020 and Sharma et al., 1979; 1998). Ugwumba et al. (2010) suggested that the higher the number of viable enterprises integrated into an agricultural system the higher the expected profit. Integrated culture system is quite compatible with the earthen pond culture and relevant to benefit the rural poor (Misra et al, 2016, Vohra et al., 2012).

INTRODUCTION Generation of solid wastes has a universal problem which are facing by most of the countries in present scenario. These wastes are generated due to several human accomplishments as well as natural disaster. It is estimated that in India nearly 700 million tons of organic wastes is generated annually included leaves, husk, sawdust, steam bark, flowers etc. Vermicomposting is a simple biotechnological process of composting, in which certain species of earthworms are used to enhance the process of waste conversion and produce a better end product. Vermicompost is nutritionally rich natural organic fertilizer, which releases nutrients relatively slowly in the soil and improves quality of the plants along with physical and biological properties of soil. It has a more beneficial impact on plants than soil. During vermicomposting, earthworms eat and grind substrates, convening with anaerobic micro flora to increase the surface area for microbial colonization and enzymatic action. Screening of locally available earthworm species and their suitability for vermicomposting during summer and winter seasons is very much required for effective recycling of available bio-waste for production of quality organic manure – vermicompost. Earthworm’s vermicompost is proving to be highly nutritive ‘organic fertilizer’ and more powerful ‘growth promoter’ over the conventional composts and a ‘protective’ farm input (increasing the physical, chemical and biological properties of soil, restoring and improving its natural fertility) against the ‘destructive’ chemical fertilizers which has destroyed the soil properties and decreased its natural fertility over the years. Vermicompost is rich in NKP (nitrogen 2-3%, potassium 1.85-2.25% and phosphorus 1.55-2.25%), micronutrients, beneficial soil microbes and also contain ‘plant growth hormones and enzymes’. It is scientifically proving as ‘miracle growth promoter and also plant protector’ from pests and diseases. Vermicompost retains nutrients for long time but the conventional compost fails to deliver the required amount of macro and micronutrients including the vital NKP to plants.