The content of the book include lectures based on the deliberations and discussions held during the faculty development programme under NAHEP grant of College of Biotechnology. The objective is the availability of these content so that these can be pursued by the academicians who teach the students and want to stay at the top of the field and keen to learn about the various best practices in the field of biotechnology in particularly in nanotechnology. The FDP included teachers, trainers, or researchers working in schools, colleges, universities, professional institutes, training centers of business enterprises, staff training institutes of central and state governments. The course included various aspects prevailing in current scenario and need to be taught to the students of agriculture and allied sciences including animal sciences, veterinary, biotechnology.

Introduction As per the reports of the United Nations Organization (UNO), the world population is expected to increase to about 9.7 billion by 2050 and to 10.4 billion by the mid-2080s. The increasing population will result in food scarcity and hunger across the world. To meet the demand for food to feed the people and meet the present aim of WHO of “Zero Hunger by 2030”, agriculture and livestock production needs to be increased. However, according to the ICAR Vision 2050 document, growth of the livestock sector is expected to be higher than the agriculture due to different factors like urbanization and changing food habits among the population residing in the urban area, lack of technology among the small and marginal farmers, reducing operational land, water scarcity, reduced irrigation infrastructure, climate change, etc. to name a few. But to achieve food security through the livestock sector, animal health and production of the animals need to be addressed.

The evolution process took millions of years to develop human and animals on the top of the hierarchy. It has moved forward due to ability to survive the various deadly diseases as for the survival of any leaving creature, it have to overcome the threat posed by the pathogens surviving in its environment and most of the time in its close vicinity. These microbes are smarter than us and are the best example of the survival of the fittest. To overcome these, nature has provided defense system to all leaving creatures that neutralize the invasion of pathogens or undesired microbes. The concept is based on the detection of self and nonself molecules.

History Pauling and his colleagues coined the phrase "molecular disease" in 1949, based on their finding that a single amino acid shift in the β-globin chain causes sickle cell anemia, which is characterized primarily by repeated bouts of acute pain due to vessel occlusion. In theory, their discoveries laid the groundwork for molecular diagnoses, though the major breakthrough came many years later. At the time, when molecular biology was only rapidly growing, providing molecular diagnostic services was unthinkable and practically impossible. In the early days of recombinant DNA technology, many scientists from different f ields collaborated to provide the first seedlings of molecular diagnostics.

Introduction Nanoparticles are minute particles with at least one dimension that measures less than 100 nanometers. They have been extensively studied in various f ields including medicine, biology, chemistry, physics, engineering, and environmental science. Nanoparticles have several unique properties, such as a high surface area to volume ratio, enhanced reactivity, and optical, electrical, and magnetic properties that differ from the bulk material. These properties make nanoparticles suitable for a variety of biomedical applications, including diagnosis, imaging, drug delivery, and therapy. This chapter will discuss the current and potential applications of nanoparticles in healthcare and research.

Cowpathy Panchgavya chikitsa is an age old system of medicine described in ancient Indian literature ‘Ayurveda’ and now it is known as ‘cowpathy’ means the therapy of human ailments through cow products. However, Cowpathy is different from Panchgavya as it also include cow cuddling, Backrub, Gau grass, Gauseva, and living with cow besides, Panchgavya products milk, curd, ghee, urine and dung. Since the cowpathy practice is to increase the aura of an individual, it prevents from many ailments and infections. In Ayurvedic system, the medicines are prepared either from plants or from animals besides the use of certain metals. The Ayurvedic medicines of animal origin are mainly prepared from indigenous cow products such as urine, dung, milk, curd and ghee. Medicinal use of cow urine has been in practice since time immemorial in India.

Introduction However, most of the crops get damaged by pest infestation, microbial attacks, and insufficient nutrient availability. Currently, the primary goal of world’s agriculture is to fulfil the nutritional demand of the rapidly growing population. Nanotechnology tries to fill the gaps in agriculture to become more resilient, while making sure there is enough food for everyone. As a result, nanoparticles are evolving into a cutting-edge material that will change how contemporary agriculture is done. The use of nanotechnology in agriculture encompasses the creation of novel materials, devices, and systems at the nanoscale to enhance the efficiency, productivity, and sustainability of agriculture. This technology has the potential to bring a revolutionary change to the field of agriculture by offering innovative solutions to various agricultural problems.

Introduction Nanocomposites are materials that incorporate nanosized particles into a standard material matrix. The addition of nanoparticles can significantly improve properties such as mechanical strength, toughness, electrical or thermal conductivity. Typically, only a small amount of material (0.5-5% by weight) is required due to the effectiveness of the nanoparticles. A nanocomposite is a solid material that consists of multiple phases, where at least one of the phases has one, two, or three dimensions in the nanometer size range. The goal of the nanoscale phase process is to achieve synergy between different constituents. Nanocomposites represent a promising alternative to overcome current limitations of microcomposites and monolithics, and are becoming the materials of the future (Schmidt et al., 2002).

Introduction The term "nano" is used to define one that has a size in the nanometer range. Nanotechnology is an interdisciplinary field of medicine, chemistry, physics, and biology that has emerged during the past few decades as a potential area of study. The goal of this interdisciplinary technology is to transform molecules into entities, then do the opposite. Nanobiotechnology is the term for the fusion of nanotechnology with biotechnology. In nanobiotechnology nanoscale principles and techniques are used to explore biosystems and generate novel biomaterials. Biosensing, cell labelling, and molecular imaging can all gain benefit from nanostructure materials in life science, health and biomedicine.

Introduction Non-coding RNAs (ncRNAs) are RNA molecules that do not code for proteins. They are transcribed from DNA and can be categorized into two main types: long non-coding RNAs (lncRNAs) and small non-coding RNAs (sncRNAs). While sncRNAs are shorter than 200 nucleotides, lncRNAs are usually longer than 200 nucleotides. Non-coding RNAs have been found to play important roles in a variety of cellular processes, including gene expression, cell differentiation, and development.

Introduction Currently, the agricultural industry is expanding exponentially. Improved economic growth and rapid population growth have spurred investor interest in the agricultural industry. The World Bank estimates it to be worth $3 trillion. With the urbanization and industrialization of the agricultural sector, the amount of waste generated has also increased, causing many environmental problems. About 5 million metric tons of biomass are produced annually from agriculture. These agricultural wastes become breeding grounds for pathogenic microorganisms if not properly treated and recycled, it is noteworthy that this agricultural waste can be used as substrates for the synthesis of valuable product or as raw material for renewable energy production.

Introduction Wheat, a vital cereal crop cultivated globally, serves as a fundamental source of sustenance and livelihood for millions (FAOSTAT, 2021). However, wheat production faces hindrances from various biotic and abiotic stresses, with heat stress being a notable challenge (Prasad et al., 2019). The term “terminal heat stress” refers to the elevated temperatures occurring during the crucial reproductive phase of wheat, encompassing the grain-filling period and flowering stages (Lobell et al., 2012). Terminal heat stress emerges as a significant impediment to wheat cultivation, particularly in arid and semi arid regions, manifesting during the reproductive stage of the crop (Wassmann et al., 2009). This predicament is exacerbated by the escalating frequency and intensity of heat waves attributable to climate change (IPCC, 2021).

Introduction Recognizing the importance and impact initiatives were taken for starting National Horticulture Mission, announced by Honorable Prime Minister on 15 August 2003. Crop diversification has become an imperative for India’s agri-economy. To address this need and to give further impetus to encouraging growth in cultivation of horticultures, vegetables, flowers, species etc. a National Horticultural Mission has been launched. Its main target will be doubling India;s horticultural production by 2010. Sub sequentially, National Horticuture Mission was launched during Tenth Plan with effect from 2005 2006 for holistic development of horticultural crops, having competitive advantages.