Poultry plays an important role in global food security as well as development economic condition by providing a readily available, affordable, and nutritious source of protein in the form of meat and eggs. It also provides income and employment opportunities, particularly in rural areas with a great contribution towards sustainable agriculture practices through the application of poultry manure as a fertilizer. In addition, poultry meat and eggs are very valuable sources of high-quality of protein that is essential for human health, Besides, poultry farming can provide a constant source of income, generation for small scale farmers in rural communities. It is well known that poultry have a relatively high feed conversion rate with a increasing demand of meat or eggs compared to other livestock. In this context, poultry health and disease and its management are well emphasized to improve productivity. The MCQ book of poultry medicine deals with both preventive and clinical medicine. The book is the valuable source of poultry medicine in which students can prepare themselves for competitive examinations like JRF/ SRF/ARS//NET, Union/State Public Service commission examination (UPSC) and Indian Forest Service’s examination (IFS). This book, is well designed to markup easy understanding and easy grasping for the readership. The book is a comprehensive guidebook for the academicians to prepare question about the subject in the academic programme. The book is prepared in such a fashion that students are not feeling bored and monotonous. A brief introduction is also included in every chapter before starting of the MCQ for better understanding of the subject. The book covers more than 2000 solved multiple choice questions with their answer keys. I hope, the book will be helpful for the undergraduate and post graduates, to prepare various competitive examinations including ICAR-JRF, SRF, NET, ARS and other competitive examination. I also welcome readers to give their opinion, criticism, suggestions, and queries for bringing out the next edition for the better improvement of the readership.

Introduction The Poultry industry stands as one of the most advanced sectors globally. The impact of diseases on poultry can be profoundly detrimental, affecting productivity, trade of live birds, meat and related products. Vulnerabilities in biosecurity within production sites, as well as weaknesses in disease diagnosis, pave the way for emerging pathogens to establish as persistent threats. In numerous tropical and developing nations poultry infections like viscerotropic velogenic New castle disease have become endemic, leading to disastrous losses. Many developing countries contend with a prevalence of vertically transmitted diseases among village-level flocks, including ailments like pullorum disease and mycoplasmosis. The combination of hot, humid climates fosters mycotoxicosis, compounded by prevalent immunosuppressive diseases, which in turn hinder the effectiveness of vaccination efforts. In the absence of robust quarantine measures and government – mandated control programs, disease can persist within both commercial and local flocks. The introduction of new infections further compounds the vulnerability of susceptible poultry populations.

1. How does the toxin of Clostridium botulinum affect the nervous system? a) Blocks inhibitory transmitter substance b) Stimulates macrophages c) Causes haemolysis and tissue destruction d) Induces systemic shock and death What is the specific effect of the toxin produced by Clostridium perfringens on host cells? a) Blocks inhibitory transmitter substance b) Induces systemic shock c) Causes ribosomal dysfunction d) Disrupts plasma membranes

1. What is a characteristic feature of fungal cell walls? a) Peptidoglycans b) Ergosterol and polysaccharides c) Antiphagocytic capsules d) Fruiting bodies 2. How do fungi reproduce in tissues? a) Binary fission b) Budding c) Simple division of round, yeast-like forms or slender, tubular hyphae d) Spore formation

Introduction Avian mycoplasmosis is caused by several pathogenic mycoplasma of which mycoplasmosis due to Mycoplasma gallisepticum (Mg), Mycoplasma synoviae (MS) and Mycoplasma meleagridis are important. The mycoplasma are group of bacteria that lack cell wall due to which the shape of bacteria is pleomorphic. Due to lack of cell wal, mycoplasma are resistant to those drug which act on cell wall and includes Penicillin and its derivatives. Mycoplasma primarily affects respiratory system and joints in birds and respiratory and urogenital system in humans. The avian mycoplasma are mainly transmitted by vertical route (egg transmission), but can also transmitted by horizontal route. Mycoplasma gallisepticum (Mg) causes chronic respiratory disease (CRD) in chicken, turkeys and game birds. Mycoplasma synoviae (Ms) causes infectious synovitis in chicken and turkeys whereas Mycoplasma meleagridis cause disease in turkey. The Mg and Ms belongs to class of Mollicutes (Molecutis: soft tissue), order Mycoplasmatales and family Mycoplasmataceae. The CRD caused by Mg affects respiratory system and air sac in presence of other respiratory pathogen/ stress or immunosuppression. Clinically, CRD affected birds show respiratory rales, gasping, nasal discharge, and rhinitis with mortality due to complication with other respiratory viral pathogens or E. coli infection. The Ms has affinity for tendon and ligaments of joint causing severe lameness due to tenosynovitis with mild lesions in respiratory system and air sac. The Ms affected birds show lameness, breast blister and increased culling of male breeder birds. The Mg and Ms causes significant losses in poultry industry due to decreased egg production, condemnation and downgrading of carcass and decreased hatchability. Mycoplasma meleagridis in turkey associated with air sacculitis and drop in egg production.

Flea of Brds Ceratophyllus gallinae – commonest flea of domestic poultry may be responsible for irritation, restlessness and even anaemia Feeds readily on humans and domestic pets and its often acquired in the handling of poultry and from injured and wild birds brought into houses could migrate into rooms from nests under adjacent caves. Echidnophaga gallinacea -‘stick tight’ flea-seen on the skin of the fowl (usually comb and wattles). Absence of genal and pronotal comb. Fleas established in a poultry house - Remove and burn all litter them spray the poultry house with an insectide. Mites of Birds

1. Prosthogonimus pellucidus is found in a) Fowl b) Duck c) Both d) None 2. Heterakis gallinarum is found in a) Fowl b) Pea fowl c) Guinea fowl d) All

Introduction Rickettsial diseases in poultry are caused by various species of bacteria belonging to the genus Rickettsia. These diseases can have significant economic implications for the poultry industry due to decreased production, increased mortality, and trade restrictions. Rickettsial diseases affecting poultry are relatively uncommon compared to other pathogens but can still occur. Rickettsial organisms are tiny, pleomorphic, weakly gram-negative bacilli that multiply by binary fission. The Rickettsia requires an arthropod for transmission and to complete its life cycle. The organisms are non-motile and non-capsulated and possess both DNA and RNA. Some of the rickettsial diseases affecting poultry include: 1. Rocky Mountain spotted fever: Chickens can be infected with various species of Rickettsia, including Rickettsia rickettsia, which causes Rocky Mountain spotted fever in humans. In chickens, rickettsial infections can lead to symptoms such as fever, lethargy, decreased egg production, respiratory distress, and neurological signs.

Introduction The protozoa (meaning proto- first and zoa mean animals) are considered to primitive and are unicellular organism. The protozoa are Eukaryotic organism having nucleus in which the genetic information is stored in chromosomes of nucleus. In poultry, the protozoan disease categorized into two main group 1. Intestinal protozoan parasites include Eimeria spp. (Coccidiosis), Cryptosporidia (Cryptosporidiosis) Histomoniasis (Histomonas meleagridis), Trichomoniasis (Trichomonas spp.) and Spironucleus (Hexamitiasis). Coccidiosis is important disease of poultry caused by protozoa of the genus Eimeria. The disease occurs by ingestion of sporulated oocyst ehcih upon action of trypsin rupture in gizzard and proventriculus relealeses sporozoites. The sprozoites penetrate mucosa of intestine and two generation of asexual reproduction occurs (Schizogony) and releases merozoites which mature and produces gamonts. Sexyual reproduction occurs (Gametogony) and produses zygot which mature to form oocysts. The oocysts are passed in faeces. The high moisture (exceeding 30%), immunosuppressive diseses and environmental and managemental stress lead to occurrence of disease. The birds affected with coccidiosis show depression, ruffled feathers, blood tinged diarrhoea and pale comb and wattle. Specific part of intestine is affected by each species of Eimeria. Diagnosis of coccidiosis is done by finding oocyst in faeces under microscope.

Introduction The control of infectious diseases of bacterial etiology in food animals is often by using collective and simultaneous medication of a group, i.e. the so-called preventive uses prophylaxis and metaphylaxis. These uses are vigorously debated because they involve the mass consumption of antibiotics that is suspected to favour the emergence of resistant bacteria, especially in the gut flora, which is critical for the potential transfer of resistance to humans via the food chain. Metaphylaxis corresponds to the administration of antibiotics to animals experiencing any level of bacterial disease before overt disease occurs, with the time of intervention depending on the detection of disease outbreaks in a few animals in the group. Metaphylaxis is launched after (meta) pathogen contamination, with the goal of the bacteriological cure of infected animals, which subsequently warrants the final protection (phylaxis) against infection outbreaks. In this respect, metaphylaxis should be viewed as a curative treatment occurring early in the time scale of the infection, and should be compared to classical curative treatments of sick animals. The pathophysiological status of the animals and the size of the bacterial load at the infection site are specificities of metaphylaxis that could play an important role in antibiotic pharmacodynamics, including the reduction of resistance selection within the pathogenic inoculum. Recent advances have been made in evaluating the antibiotic doses required for clinical and bacteriological cures, depending on the size of bacterial load at the infection site. The observations that total doses were much lower for early treatments when bacterial loads were small, compared to later treatments of sick animals with higher bacterial loads, reinforces the in vivo relevance of the so called ‘inoculum effect’, whatever its underlying mechanisms.

Introduction The quality of interaction between clinicians and microbiologists has an enormous influence on the effectiveness of the laboratory service. An accurate diagnosis is based on the interpretation of both clinical and laboratory data. Investigation of disease is solely dependent on the quality and appropriateness of the specimens collected. The diagnosis depends on the skill and care with which the clinicians select, collect and transport the specimen to the laboratory. Clinical history, including the tentative diagnosis, should always accompany the specimen. If available, a detailed post-mortem report must also be sent. These set of information will help the microbiologist to select the most appropriate procedure and to furnish a meaningful interpretation of the results. Specimens need to be collected for establishing a disease diagnosis or monitoring of vaccine response or surveillance. The knowledge of the pathogenesis of the infectious disease is the most important factor for determining the most suitable specimen. The samples need to be appropriate, and adequate in number and amount to provide a statistically valid result. Samples must be taken with care, to avoid undue stress or damage to the bird or danger to the operator. Careful consideration must be given to the collection, containment, and storage of the specimens, including biosafety measures to prevent spillage to the environment or exposure of other birds and humans.

Introduction The current health situation in poultry farming is dotted with cases where these emerging or re-emerging diseases attack birds. A large majority of them are of viral origin and a powerful immunosuppressive effect is observed, sometimes accompanied by bacterial infections. Most of the important infectious diseases of poultry have been known for many years and are controlled by medication, vaccination, eradication and bio-security. Examples of such diseases that were known before the intensive commercial poultry industry development are infectious bronchitis (IB), newcastle disease (ND), infectious bursal disease (IBD), lymphoid leucosis and so on. From time to time, new diseases emerge to provide fresh challenges for diagnosis, control and understanding of their epidemiology. World Health Organization (WHO) definition of an emerging disease is defined as a disease that has appeared in a population for the first time, or that may have existed previously but is rapidly increasing in incidence or geographic range. Diseases that reappear after a period of absence can be considered as re-emerging. Some of these include runting/ stunting syndrome and inclusion body hepatitis. In addition, other well-established diseases evolve in different ways and can also present problems for diagnosis and control. In the wider sphere, it is recognized that emerging viral infections in both humans and animals have been reported with increased frequency in recent years. Examples are SARS, avian influenza, West Nile virus and the Nipah virus. The more recent diseases of viral origin, others that have re-emerged and some experiences from previous emerging diseases are living examples for threat to the poultry industries. In addition, infectious bronchitis is an example of a disease which the causal virus is showing constant evolution. The impact of infections caused by the avian metapneumovirus (aMPV) is an example of recently emerged viral agent.

Any abnormal condition, which affects the normal functioning of cells, tissues, organs and any other system in an individual, is called a disease. In all living organisms, including plants, animals, birds and humans, diseases can be caused due to infectious or non-infectious agents and various other factors. Laboratory tests may identify organisms directly (eg, visually, using a microscope, growing the organism in culture) or indirectly (eg, identifying antibodies to the organism). Some tests (eg, Gram stain, routine aerobic culture) can detect a large variety of pathogens and are commonly done for many suspected infectious illnesses. However, because some pathogens are missed on these tests, clinicians must be aware of the limitations of each test for each suspected pathogen. In such cases, clinicians should request tests specific for the suspected pathogen (eg, special stains or culture media or advise the laboratory of the suspected organism(s) so that it may select more specific tests. The main causative factors of diseases in humans include internal factors, genetic irregularities, allergies, poor immune system, external factors and a lot more.

Introductioin Poultry diseases can be caused by bacteria, viruses, fungi, parasites, or a combination of these pathogens. Common poultry diseases include Newcastle disease, avian influenza, infectious bronchitis, infectious bursal disease, coccidiosis, and salmonellosis, among others (Grace et al., 2024). These diseases can affect various organs and systems in poultry, including the respiratory, gastrointestinal, and immune systems, leading to a wide range of clinical signs and symptoms (Dunislawska et al., 2024). Prevention and control of diseases in poultry is paramount for maintaining the health and welfare of poultry f locks and ensuring the sustainability of the poultry industry. Poultry diseases can have devastating effects on production, leading to decreased productivity, increased mortality rates, and significant economic losses. Therefore, implementing effective prevention and control measures is essential for poultry producers to mitigate the risks associated with diseases (George & George, 2023).

Introduction A thoroughly performed physical examination in poultry and avian clinical practice is of great importance. Most avian species happen to be prey animals, with the exception of a few raptors that tend to be predatory. This makes them try to appear healthy, in preparation to escape from potential predators, and as such, makes it difficult for their owners, keepers and veterinarians to detect early diseases. Physical examination when carefully performed in detail, increases the chances of obtaining fewer differential diagnosis that are likely to be harboring the confirmatory diagnosis after laboratory works are being performed. In essence, a thorough physical examination brings about cost efficiency in poultry/ avian clinical practice. Take for instance, a farm with over 50,000 layers, having lowered productivity. It will be cumbersome to go ahead and start blood sampling, faecal sampling or oral cavity swabs for laboratory analysis as the starting point. The disadvantages will be high labour demands, high capital demands, more stress on the animals and of course, long time till feasible outcome is to be obtained.

ELECTROLYTE BALANCE Electrolytes like sodium, potassium, and chloride are substances that dissolve into posi tively and negatively charged particles when mixed with a liquid. The interaction between these substances, referred to as the ‘dietary electrolyte balance (DEB), can be influenced by either the electrolyte itself or the additional salt source used. Dietary inclusion of potassium and chloride has shown positive effects on heat-stressed broilers. Among various salt options for broiler diets, sodium bicarbonate and potassium chloride have been identified as the optimal choices, especially during hot summer conditions. Electrolyte balance is often quantified by the uncomplicated equation Na + K – Cl expressed in terms of mEq/kg of diet. An ideal overall dietary equilibrium ranging from 240 to 260 mEq/kg is generally deemed optimal for normal physiological functioning. The body’s buffering systems play a pivotal role in maintaining a close-to-normal physiological pH, thus preventing deviations in electrolyte levels. The principal function of electrolytes pertains to the regulation of body water and ionic equilibrium. Consequently, the requisites for elements like sodium, potassium, and chloride can’t be viewed in isolation; it’s the collective equilibrium that holds significance. The term “electrolyte balance,” synonymous with acid-base balance, is influenced by three factors: 1. The equilibrium and proportion of these electrolytes in the diet. 2. Endogenous production of acids. 3. The pace of renal clearance.

Introduction Knowledge of the avian respiratory system is essential for developing a health monitoring plan for a poultry flock, recognizing problems that may occur, and taking action to correct them. The avian respiratory system begins with nostril includes trachea, bronchi (bronchus) and ends with lungs and air sacs. Respiratory diseases are the most common cause of death in a poultry flock. The avian respiratory system is involved in the following functions: • Absorption of oxygen (O2) • Release of carbon dioxide (CO2) • Release of heat (temperature regulation) • Detoxification of certain chemicals • Rapid adjustments of acid/base balance • Vocalization

Introduction Poultry are domesticated birds mostly reared for the purpose of meat and egg consumption as well as for their feathers. It includes chickens, quails, turkeys, waterfowls, ducks and geese. The endocrine system of poultry consists of hypothalamic-hypophyseal complex, parathyroid gland, thyroid gland, pancreatic islet cells, adrenal glands, ultimobranchial glands, endocrine cells of gut and the gonads. These endocrine organs release hormones into the bloodstream. Peptide hormones act on the surface of cells, whereas steroid hormones act on target tissues by entering in the cytoplasm or nucleus of cells. Lack of diagnosis in the case of endocrinopathies is a major cause of production loss globally. Advancement in awareness, diagnosis, treatment as well as management is critical to ensure optimal flock health. Cost-effective programs of biosecurity and vaccination are required to prevent or limit the impact of disease. Programs of emergency treatment and long-term prevention are justified for severe endocrine diseases which have a profound impact on production.

Introduction Ante mortem diagnosis of cardio vascular diseases in avian patients is a challenging to the physician. Compare with the mammals, few anatomical and physiological features are unique in birds. The avian heart is anatomically similar to mammals, although birds have a proportionally larger heart size relative to a comparably sized mammal. Heart size in mammals remains equivalent to body mass, while larger birds have a proportionally smaller hearts in relation to body size than smaller birds. The apex of the heart is enclosed between the right and left hepatic lobes and lies along the sternum and parallel to the thoracic spine in the cranio ventral coelom. The avian cardiovascular system is adapted to the high aerobic requirements for flight, running or swimming. Efficient oxygen transfer to the tissues is facilitated by a lower total peripheral resistance, higher heart rate, higher arterial blood pressure and more rapid myocardial depolarization. The clinical signs seen in birds afflicted with cardiac disease can be vague and non-specific which are mimicking many other disease conditions. Similar to mammals, birds may present with lethargy, weakness, exercise intolerance, syncope, dyspnoea, coughing and even sudden death. On physical examination cardiac disease may be suspected based on the presence of arrhythmia, murmur, muffled cardiac sounds, poor peripheral pulses, cyanosis and coelomic distension. Pulmonary oedema, hepatomegaly, ascites and enlarged jugular veins with pulsation may be seen in cases of congestive cardiac failure. Pericarditis can occur with primary infectious agents or secondary to disease occurring in adjacent tissues, such as mycotic pulmonary granulomas. Visceral gout due to hyperuricaemia may also affect the pericardium. Congestive heart failure and systemic manifestations of disease, such as hypoproteinaemia, may result in effusions within the pericardium. Myocardial ventricular hypertrophy can occur with any condition that results in an elevated preload on the heart, including pulmonary hypertension and atherosclerosis. Myocarditis has been observed in cases of chlamydiosis, polyomavirus infection and proventricular dilatation disease from bornavirus. Heavy metal toxicosis has been associated with cardiac neural dysfunction and myocardial infarction. Vegetative endocarditis and resultant valvular insufficiency can occur with bacteraemia from chronic bacterial infections involving other organ systems.

Introdcution Poultry have a nervous system made up of the brain, spinal cord, sympathetic nerves that govern their viscera, and branches that go to their ears and eyes. The structure of the brain and spinal cord is very similar to that of mammals. However, there are also significant structural variations between many bird brains and many human brains, including the following: 1. Absence of cortex: According to Cobb (1960), the cortex is “a peripherally placed coating (pallium) of cells arranged in layers,” yet it is completely absent in birds. However, other authors have compared the hyperstriatum to the mammalian cortex (Haefelfinger, 1957; Stingelin, 1958). 2. Presence of hyperstriatum: The functions of the hyperstriatum are comparable to those of the mammalian cortex. In pigeons and chickens, the hyperstriatum may be involved in the visual and hearing systems’ functions. 3. Birds have highly developed optic lobes. 4. Turkeys and vultures are the only birds with a well-developed olfactory system.

Introduction The digestive system of the birds has modifications to facilitate the easy flight. Length of intestine in birds is relatively shorter compared to mammals. Also, they lack teeth and jaw muscles which are replaced with light weight beak. Food grains are swallowed as whole and ground in the gizzard with the help of powerful muscles. In the mouth, tongue is used to manipulate the food and also aids in swallowing the food. Chickens have as many as 300 taste buds. The elementary system in birds consists of gastro intestinal tract and the accessory glands, the course of the elementary starts with Mouth, oesophagus, crop, proventriculus (glandular/true stomach), gizzard (mascular stomach), small intestine (duodenum, Jejenum, ileum), large intestine (colon and caeca) which lastly opens into cloaca. For fast growing poultry species elementary system is very important. It plays vital role in digestion, absorption and also provides protective immunity. Poultry intestines harbours diversified microflora which aids in enhanced availability of nutrients to the bird. Intestinal wall contains four layers: mucosal, submucosal, muscle tunic, and the serosal layer. As intestine is involved in important functions like digestion & immunity, any change in intestinal integrity / microflora balance impacts the bird performance in terms of body weight gain in broilers and egg number in layers. Some bacteria, viruses and also fungi produce different diseases / conditions in poultry birds. The ban on the use of antimicrobial growth promoters has pushed poultry producers from use of AGP’s to alternatives to AGP’s such as prebiotics, probiotics, essential oils and some herbals. Maintaining good gut health in the absence of AGP’s and anticoccidial drugs is a challenging situation for the poultry farmers across the globe. Poultry gut contains diverse community of bacteria, fungi, protozoa and viruses. The recent data suggests that gastrointestinal tract of a broiler chicken colonizes by an estimated 600 – 800 species of bacteria. The abundance and diversity of microbiota varies along the GI tract. After the hatch GI tract colonizes within 3-4 days. Small intestine of broilers mainly contains lactobacilli although enterococci, E.coli, eubacteria, clostridia, propionibacteria and fusobacteria can be found.

Introduction Hepatocytes are tiny cells that constitute the primary structural unit of the liver. Periportal hepatocytes, the first to receive blood from the portal system, are particularly vulnerable to hepatotoxins and endotoxins. Periacinar hepatocytes, placed further away from the portal blood supply, are more susceptible to hypoxia, toxic metabolites and hypoperfusion. Inflammatory cells are drawn to injured hepatocytes and biliary epithelium due to the release of cytokines and inflammatory mediators. This causes additional hepatocyte necrosis and fibrosis. The liver has exceptional regenerative abilities as hepatocytes can be replaced even if only 12% remains intact. Hepatocytes exhibit colour changes when they are laden with fat or pigments. In their f irst week of life, young turkeys and chickens typically have a lot of fat and pigments (carotenoids) deposited in the liver due to the ability of intestines in mobilizing the yolk content. It is typical to find fat vacuoles (microscopic fat deposits) within the hepatocytes at this point. A bird's liver will typically turn mahogany-brown after about seven days of life. Because of the influence of oestrogens, the hormones that are present in large concentrations once maturity is attained, the quantity of fat in the liver of adult hens increases before the age of lay. Because a large amount of fat and pigments are transferred from the liver to the oviduct for yolk formation in eggs, the majority of laying hens in a flock will typically have a pale brown or yellowish liver. When liver seems pale or yellow, histopathology is a crucial technique that can be used to establish a differential diagnosis.

Introduction Musculoskeletal diseases in poultry are often exhibited as lameness or leg weakness. Numerous factors, including those related to diet, animal husbandry, infections, or heredity, might contribute to musculoskeletal problems. Lameness can be exhibited due to disorders in the central or peripheral neurological systems, reproductive system, trauma or infection in the foot, leg, hip, spine, or just a generalised sickness. Less frequently, musculoskeletal anomalies can affect the bird’s beak, neck, or wings. The diseases can also be classified as infectious origin (bacterial, viral) or non-infectious origin (congenital deformities, nutritional, trauma or neoplasia). Modern poultry production lines put a lot of strain on the musculoskeletal system due to factors like growth rate in broiler chickens and egg output in laying hens. As a result, poor nutrition and husbandry practices frequently lead to musculoskeletal disorders. Thus, musculoskeletal disorders in poultry can be controlled by choosing proper genetic selection, efficient vaccinations, adequate nutrition and proper husbandry practices.

Introduction The urinary system of poultry, consisting of the kidneys, ureters, and cloaca, is essential for removing waste products and maintaining fluid balance. However, this system can be susceptible to various disorders that can impact the health and productivity of the flock. This chapter will discuss some of the most common urinary system disorders in poultry. Urinary system disorders can have significant negative impacts on poultry health and productivity (Klybeck, n.d; Mississippi State University Extension, n.d; Poultry Hub, n. d.). Some of the key ways these disorders affect poultry include: 1. Gout: Renal diseases often lead to gout in poultry, which is a metabolic disorder resulting in hyperuricemia and deposition of uric acid or urates in tissues (Klybeck, n.d.). This can further damage the kidneys or other body systems. 2. Reduced productivity: Gout has a direct effect on productivity by lowering weight gain, increasing feed conversion ratio (FCR), and causing mortality which may reach 11-30% (Klybeck, n.d) 3. Organ damage: Accumulation of uric acid can lead to swelling and damage of kidney cells, as well as visceral gout where whitish deposits are found on the surface of organs like the heart, liver, intestines, and lungs (Klybeck, n.d; Mississippi State University Extension, n.d.). 4. Mortality: Severe kidney damage and dysfunction can quickly lead to debilitation and death in poultry (Poultry Hub, n.d.). Acute septicemic coliform infections may also cause sudden death (Mississippi State University Extension, n.d.) 5. Respiratory issues: Infectious bronchitis virus can affect both the respiratory and urinary systems, causing inflammation and damage to the kidneys (Mississippi State University Extension, n.d.).

Poultry, encompassing a wide range of domesticated birds, plays a crucial role in agriculture, providing meat and eggs to populations worldwide. The health and well-being of poultry are of paramount importance for sustainable and efficient production. One aspect that significantly influences poultry health is the condition of their wings. Wings serve various purposes for birds, including flight, balance, and protection. Disorders affecting poultry wings can arise from a multitude of factors, ranging from nutritional imbalances to infectious agents and environmental stressors. Understanding and addressing poultry wing disorders require a comprehensive approach that encompasses both preventive measures and therapeutic interventions. This comprehensive understanding involves recognizing the various disorders that can afflict poultry wings, their causes, symptoms, and potential treatments. In this exploration, we delve into 50 multiple-choice questions and answers covering a diverse array of poultry wing disorders, providing insights into the complexity of avian health management. The Significance of Poultry Wing Health Maintaining the health of poultry wings is not merely a matter of ensuring their ability to f ly; it is intrinsically linked to the overall well-being of the bird. Poultry wings are vital for performing a myriad of daily activities, including foraging, preening, and maintaining social hierarchies within flocks. Additionally, the condition of the wings often reflects the bird’s general health status, making them an essential diagnostic indicator for avian health professionals.

Introduction The reproductive system is fundamental to poultry production, playing a crucial role in both the quantity and quality of eggs laid. Its importance goes beyond mere biology, impacting the profitability and long-term sustainability of poultry operations (Abbasi et al., 2024). Central to this is the egg, the ultimate product of avian reproduction. The reproductive system coordinates complex processes to ensure eggs meet high standards. Any disruption to this delicate balance can have far-reaching effects on the economic and environmental aspects of poultry farming. Disorders affecting the avian reproductive system pose significant challenges to the poultry industry. Whether they are minor disturbances or major disruptions, these disorders can undermine the foundation of successful production. They can lead to reduced egg production and compromised egg quality, affecting productivity, efficiency, and ultimately, profitability. In today’s poultry management landscape, where efficiency is paramount, the impact of reproductive disorders cannot be overstated. Beyond economic concerns, these disorders also affect sustainability and environmental responsibility. A healthy reproductive system is not only crucial for financial success but also for upholding ethical and responsible poultry practices. As such, ensuring the reproductive health of poultry is of utmost importance. It requires dedicated effort, attention to detail, and a comprehensive approach. By strengthening the reproductive system against various threats, poultry producers can secure not only the current success of their operations but also a legacy of responsible stewardship for future generations (Habibi et al., 2024).

Introduction Pain management in poultry is an important aspect of animal welfare and overall production efficiency in the poultry industry. Poultry birds, such as chickens and turkeys, can experience pain due to various reasons including routine management procedures, injuries, and diseases. Managing pain in poultry is crucial not only for ethical reasons but also because pain can lead to decreased productivity and increased susceptibility to diseases. For the better management First step is identification of pain in birds which can only be possible with the thorough knowledge of their behaviour. Second step is the selection of appropriate analgesics and their dosing’s as there are various species of birds. Commonly used analgesics for birds includes, NSAID’s, opioids etc. 1. Routine Management Procedures: Many routine procedures in the poultry industry, such as beak trimming, toe clipping, and wing clipping, can cause pain and distress to birds if not done properly. Beak trimming, for example, is often performed to reduce feather pecking and cannibalism in crowded conditions but can cause acute pain if not done correctly. Proper techniques and pain management strategies must be employed during such procedures to minimize pain and distress in birds.

The skin of the birds provides a physical barrier between the birds its environment, protecting the birds from bacteria and physical injury. The health of the bird can be compromised, and the value of the end-product can dramatically decrease. Bird’s skin differs from that of mammals by its thinness, by the presence of feathers instead of hair, and by the absence of sebaceous glands (sweat glands), although the overall histological structure is similar. Bird’s skin is composed of an epidermis separated from a dermis by a basal membranes to protect the body from infection. The integumentary system consists of the skin, the feathers and the appendages (claws and beak). The skin covers the majority of the body and contains glands in the outer ear canal and the preen gland at the base of the tail, that the bird uses to preen its feathers. The integumentary system is very important in providing protection to the bird from a number of potentially dangerous situations. The functions provided by the integumentary system include: A barrier between the external environment and the internal systems and organs thus provides support and protection from infection by microorganisms and from physical injury. Excellent thermal insulation to help regulate body temperature in a variable environment. Numerous nerve endings for the senses to enable the bird to be aware of potentially harmful situations. Pigments for display and protection from the elements. The compounds capable of conversion into vitamin D when exposed to sunlight. Avian skin is relatively thin with the epidermis consisting of only 2-10 cell layers. There are no sweat glands; instead heat is lost via the respiratory tract and by radiation from featherless areas. The brood patch is a featherless area that can occur in both sexes and is either seasonal or permanent depending on species.

Introduction Eye Avian ocular apparatus includes eyeball, optic nerve, and accessary structures e.g., eyelids, lacrimal glands, and muscles etc. The eyeball is composed of three tunics namely the f ibrous (cornea and sclera), vascular (choroid, iris, ciliary body) and nervous tunic (retina). The histological structures of all these tunics are similar to mammals except the Bowman’s membrane (the basal lamina of corneal epithelium) is not distinct. Similar to the mammals, they also have three refractive media viz., aqueous humour, lens, and vitreous humour. The cornea does not refract light. There are some important diseases and disease conditions of eye in poultry birds. The common aetiology of eye diseases in poultry birds has a wide range and includes heredity, nutritional deficiency, infections (microbial and parasitic) and managemental failure. Some of them are not seen often in the modern intensive rearing systems like the deficiency diseases while others are seen associated only with the intensive system housing of birds like the ammonia burns. The infectious diseases of only eyes, are not encountered in poultry birds. The diseases like the colibacillosis, pasteurellosis and Marek’s disease can affect eyes along with other body systems. Understanding of eye diseases of poultry birds requires basic knowledge of ophthalmic anatomy, concepts and terminology of eye associated pathological changes. The affected birds generally show ocular lesions and loss of condition due to starvation as they are unable to search for feed and water. Some conditions are reversible while others may be irreversible according to the extent of damage. Nutritional and managemental diseases in most cases are relatively easy to diagnose and manage, while infectious and hereditary diseases may require deeper investigation. Some diseases like endophthalmitis, eye notch syndrome and ophthalmopathy have been recorded but their causes could not be found out.

Introduction Disorders of the hemopoietic system in poultry can significantly impact the health and productivity of these birds. The hemopoietic system is responsible for the production of blood cells, including red blood cells, white blood cells, and platelets. Disorders within this system can lead to various health issues, including anemia, immunodeficiency, and impaired blood clotting. Some of the common disorders of hemopoietic system include: 1. Anemia: Anemia is a condition characterized by a decrease in the number of red blood cells or hemoglobin in the blood. In poultry, anemia can be caused by various factors, including nutritional deficiencies (such as iron deficiency), infectious diseases (such as avian malaria or chicken infectious anemia), and parasitic infestations (such as blood-sucking parasites like leucocytozooncaulleryi, ticks, lice and mites). 2. Avian Leukosis:Avian leucosis is a viral disease caused by avian leukosis virus (ALV). It primarily affects young birds, causing tumors in various organs, including the spleen, liver, and bursa of Fabricius. Avian leukosis can lead to immunosuppression, anemia, and increased susceptibility to secondary infections. The avian leucosis diseases include lymphoid leucosis (affecting lymphoid cells), myeloid leucosis (affecting myeloid cells), osteoptrosis, etc. and can result in immunodeficiency, increased susceptibility to infections, and other systemic symptoms. It also affects blood-forming tissues, including the bone marrow and lymphatic system, leading to an overproduction of abnormal white blood cells.

Metabolic disorders are classed as illness associated with a failure in one of the body hormone or enzyme systems, storage disease related to lack of metabolism of secretory products because of the lack of production of a specific enzyme, or the failure or reduced activity of some metabolic function. There are numerous genetic, metabolic disorders in poultry but these are rare in commercial poultry. In poultry it is usual to include under the heading of metabolic disorders those conditions associated with increased metabolism, rapid growth rate or high egg production that result in the failure of a body system because of the increased work-load on that organ or system. Metabolic disorders that result from an increase production of, or deficiency of or failure in the production, synthesis, or transport of an enzyme, hormone or secretary mechanism. Metabolic disorders that result from high nutrient intake, rapid growth, high metabolic rate, pulmonary or systemic hypertension, and high egg production or a rapid increase in egg production. Other conditions that could be classed as metabolic disorders related to: (a) management defects; (b) nutritional deficiency or excess; (c) infectious agents; (d) toxins.

Nutritional deficiencies can result from a lack of specific nutrients in the diet, adverse interactions between nutrients in seemingly well-balanced diets, or the influence of specific anti-nutrients. The latter two scenarios are particularly challenging to diagnose because dietary analysis may suggest that nutrient levels are adequate. Micronutrients such as vitamins and trace minerals are typically added to diets through standalone micro premixes, making classic signs of individual nutrient deficiencies rare. Instead, the effects are often a combination of various metabolic conditions. Accurate diagnosis often requires comprehensive information about the diet and management practices, clinical signs in affected birds, necropsies, and tissue analyses. However, tissue analysis, especially of the liver and serum, can be misleading. Following the onset of a deficiency, birds may sequester nutrients in the liver, leading to falsely high liver assay values even when the diet is deficient. This effect is particularly significant for minerals such as copper. A diet that appears to contain sufficient levels of certain nutrients based on analysis may actually be deficient in those nutrients to some extent. Stressors such as bacterial, parasitic, or viral infections, as well as extreme temperatures, can interfere with nutrient absorption or increase the nutritional requirements. Toxins or microorganisms may destroy or render unavailable specific nutrients that appear to be present in adequate levels according to conventional chemical or physical assays. Consequently, many trace minerals and vitamins are included in the diet at levels significantly higher than the actual requirements to account for these potential issues.

Paracelsus recognized more than 400 years ago that it is “the dose that makes the poison.”Deliberate or inadvertent over dosages may cause illness, and a misplaced decimal in water or feed medication concentrations frequently results in toxicity. A general feature of modern complex poultry rations, feed mill equipment, and feed delivery to poultry farms is that any component included in a ration may at some time be mistakenly included at a higher than desired rate. This may occur through human or mechanical error. For example, sulfaquinoxaline poisoning occurs in meat-type chickens, even at recommended doses, because of high water intake in warm buildings, particularly in hot weather, or because of poor feed mixing. Poisonous substances are widely distributed in nature. Poisoning occurs more frequently in free-range and backyard flocks and in village poultry where birds forage in neighboring gardens and fields or receive household waste and weeds cut from roadsides and fields. Some of these poisonings are malicious. Contaminated litter on the floor and in nest boxes is an added source of toxins in chickens not raised on wire.

All infectious agents, toxins, and nutritional imbalances have an impact on the performance of the farm and consequently on the local poultry industry. Additionally, poultry can be infected with common diseases like endoparasites, ectoparasites, infectious bronchitis, Marek’s disease, fowl cholera, salmonellosis, infectious coryza, fowl pox, avian encephalomyelitis, etc. Controlling infectious diseases is vital for poultry health and diagnostic methods are an indispensable feature to resolve disease etiologies and the impact of infectious agents on the host. Although the basic principles of disease diagnostics have not changed, the spectrum of poultry diseases constantly expanded, with the identification of new pathogens and improved knowledge on epidemiology and disease pathogenesis. In parallel, new technologies have been devised to identify and characterize infectious agents, but classical methods remain crucial, especially the isolation of pathogens and their further characterization in functional assays and studies. In poultry medicine, the diagnostic process originally shifted from the traditional veterinarian approach centered on individual animals to the health assessment of entire flocks. Flocks are commonly classified as “healthy” if they perform according to their genetic potential and are considered free from clinical disease. On-farm, diagnostic activities comprise routine sampling and investigations in line with health control programs; nationally and/or internationally adopted control programs for certain Mycoplasma and Salmonella species represent examples of paramount importance. Samples may be investigated immediately on site (e.g., rapid antigen test for avian influenza) or sent for further processing to a laboratory (e.g., ELISA and PCR). Field veterinarians further implement diagnostic surveillance in order to provide epidemiological data for flock management purposes. The periodical collection of samples (e.g., feces, serum samples, and swabs from mucosal surfaces) is primarily used to confirm the infection (free) status of a flock or to monitor vaccine response. Altogether, generated data facilitate objective judgment and decision making in order to optimize flock health and production.

In avian medicine, because clinical signs in birds can be vague, veterinarians must rely on additional clinical tests to accurately diagnose and treat conditions. These tests, including hematology, urine analysis and others, are essential for understanding diseases early and tailoring effective treatments. Clinical hematology is qualitative and quantitative assessment of blood and other blood component for the treatment of clinical patient. In comparison to mammals, bird’s erythrocytes are oval and nucleated cells, nucleated thrombocytes, heterophiles instead of neutrophils. RBCs of birds have relatively short life span and regenerate more quickly than mammals. The preferred vein for blood collection in birds varies with the species. In most species the wing veins is preferred site. Both EDTA and heparin have been used in avian clinical haematology as anticoagulant. The average blood volume of most birds is approximately 10% body weight. Avian plasma samples frequently are yellow due to carotenoid pigments, not bilirubin. Uric acid is the major nitrogenous waste product of birds it is relatively inert and substantially less toxic than ammonia or urea. Both BUN and creatinine levels are normally low in birds and may be below the minimum detectable limit of the assays in the laboratory. It may be useful to evaluate BUN and uric acid together to differentiate among dehydration, postprandial effects and renal pathology.

Introduction Poultry is one of the most widespread food industries worldwide. Chicken is the most common type of poultry in the world. The term broiler is applied to chickens that have especially been bred for meat; they grow rapidly. Broiler strains are based on hybrid crosses between Cornish White, New Hampshire and White Plymouth Rock. In broiler production there are two main production phases – keeping of parent stock and production of day-old-chicken (DOC); and growing and finishing of broilers. The primary purpose of any enterprise is to maximize return on investment over the long-term. It is therefore necessary to market poultry, meat products, and eggs at a price which allows farmers or integrators to maintain profitability in a competitive market. Cost-effective programs of biosecurity and vaccination are required to prevent or limit the impact of disease. It is emphasized that the incremental return in the form of enhanced egg production, hatchability, liveability, growth rate, and feed conversion efficiency must exceed capital and operating expenditures on disease prevention.