Preface Agriculture and livestock are interdependent and synergistically contributing to economic growth of the country. Livestock contributes around one fourth of the total GDP coming from agricultural sector. About seventy-five per cent of the rural masses are associated with livestock rearing and earn their livelihood partially/wholly from this sector. Although, we are growing very fast in livestock production for milk, egg, meat etc, yet at the same time are becoming more dependent only on a few high producing livestock breeds. India is endowed with rich livestock diversity having 151 well-established indigenous breeds of livestock across all species till date and many more still to be recognised which are adapted to varying climatic and abiotic stress conditions. However, as a result of indiscriminate crossing of the indigenous breeds with exotic germ plasm or their mixing with the local breeds, there is a concern of losing pure breeds. India possesses one of the largest livestock wealth in the world. The total livestock population consisting of Cattle, Buffalo, Sheep, Goat, Pig, Horses & Ponies, Mules, Donkeys, Camels, Mithun and Yak in the country is 512.05 million (Livestock Census 2012). According to 19th Livestock Census, cattle population was 190.90 million (37.28%), buffalo 108.70 million (21.23%), sheep 65.10 million (12.71%), goat 135.17 million (26.40%) and pigs 10.29 million (2.01%). The corresponding percentage wise figures as per 18 Census, were 37.58%, 19.89%, 13.50%, 26.53% and 2.10%. Mithun, yaks, horses, all together made-up of 0.37 % of the total livestock. There was a positive growth in horses & ponies with 11.68% increase and mules with 13.26% in urban areas. Globally, the livestock diversity has become more vulnerable to genetic erosion probably due to monoculture of high performing breeds in response to increasing market demand. Ever changing utilization pattern has shrunken the base of some of the most important livestock breeds. The widely distributed and diversified genetic resources are also underutilized because of one reason or the other. Our indigenous livestock is well acknowledged worldwide for possessing genes for unique attributes or combinations of characteristics like disease resistance, tolerance to climatic extremes particularly heat tolerance and better thriving ability, which will become more and more important for sustainable animal production in future. However, at the same time, we have to devise new methodologies for better and diversified utilization of our livestock as per the changing market demands and social patterns. This book is based on the conference on “Integrated Development of Vast Biodiversity of Indigenous Livestock for Long Term Rural Livelihood Security” which was organized by the department of Animal Genetics and Breeding, College of Veterinary in collaboration with the Society for Conservation of Domestic Animal Biodiversity on February 7-8, 2013. The society organizes this event every year at different institutions in the country which are engaged in development of livestock and animal welfare. After peer review, 233 research papers were accepted for presentation during the symposium. Later on, 25 papers were selected for publication in this book. There are 25 chapters in total in which the distinguished contributors have made valuable contribution to the field of conservation of animal biodiversity and improvement of livestock. The selected manuscripts were further improved and the edited book presents the topics on cattle genetic resources, strategic action plan for sustainable management, conservation and genetic improvement, breeding strategies to improve milk productivity, production systems and breeding policies for genetic improvement, application of biotechnological tools for conservation and improving of animal genetic resources, bioinformatics and genome analysis in conservation, heat stress management etc. The contributors to this book deserve special thanks as it is only because of their valuable contributions due to which publication of this book could be possible. The contents of the book are as per the material provided by the contributors and the editors shall not be responsible for any controversy. The editors duly acknowledge the assistance provided by Shri Shailendra Singh, Laboratory Technician in the department of Animal Genetics and Breeding for compilation of this book.

In Vedas the cow has been regarded as the mother of Rudras, the daughter of Vashu and the sister of Aditayas and also the use of cow ghee in religious ceremonies had been mentioned in Rig Veda and there is mention of the cow as “Kam Dhenu” in ancient writings. In Shiva temples are found the references of sculpture of “Nandi Bulls”. In Hindu mythology, the male buffalo thought to be the vehicle of the God of death “Yama” and also regarded as physical form of the demon Mahishasur, a symbol of inertia-”Tamas”.

Animal husbandry is an integral component of Indian agriculture supporting livelihood of more than two-third of the rural population. The livestock sector plays a significant role in supplementing family incomes and generating gainful employment in the rural sector, besides providing cheap nutritional food to millions of people. Livestock provides milk, egg, meat as nutritious food, draught power for agriculture, wool, fibre, manure and domestic fuel, hides & skin. In 2010-11, this sector contributed 121.84 million tonnes of milk, 63.02 billion eggs, 42.99 million kg wool, and 4.83 million tonnes of meat. Milk is the main output of livestock sector accounting around two third (67%) of the total output by livestock sector. Meat and egg share 17.5% and 3.6% of the value of livestock output. Livestock contributed 4% to the total GDP and 26% to the agricultural GDP in 2010-11. Livestock are the best insurance against the vagaries of nature like drought, famine and other natural calamities.

Dairy farming has been the backbone of the agriculture and rearing of livestock for milk/milk products and draft power has been the tradition of ancient rural India. Whenever the crops have failed miserably attributed to natural calamities, the livestock has emerged the savior of farmers providing livelihood security in rural India. India is a country of villages (640930 villages) and 68.84% population of India is living in villages as per the All India Census, 2011. Further, 41.8% rural population is below poverty line as compared to 25.7% urban population (Basic Animal Husbandry Statistics, 2012). The marginal and small farmers are rearing 71.7% of total livestock revealing that livestock has been the source of sustainable livelihood security for rural poor majority of which falling under the category of population below poverty line.

India produced 121.8 MT milk during the year 2011-12 and continued to rank first in world milk production. India contributed 16 % of world milk production. As per 2010 statistic, India with 12.5 % of world cattle population contributed 8.4 % in world cow milk and  with 56.7 % of world buffalo population contributed  67.45 % in world buffalo milk. In total milk production of India, contribution of crossbreds, indigenous cattle and buffalo were 23 %, 20% and 53%, respectively.

Introduction India ranks third in sheep population and accounts for 6.13 % of world population (FAO, 2009) with 71.56 million sheep to its record as of Livestock Census 2007 (DAHD, 2012). Out of 71.56 million sheep, 3.73 million are crossbred and 67.83 are indigenous. Sheep husbandry is backbone of rural economy in India. It is important as it helps in sustaining the livelihood of rural poor in difficult terrains characterized by sparse vegetation, marginal land and a high incidence of poverty. These breeds were developed following domestication and natural and artificial selection over the past 12,000 years.

Hill cattle in Uttarakhand are multipurpose animals. They are used for milk, draft, manures and ceremonial purposes. Improvement for these attributes depends on definition and subsequent selection for various traits. Based on the criteria for improvement and detailed literature analysis, it is believed that the hill cattle of Uttarakhand is one of the potential population for milk in the state specially in the remote and high altitude areas.. Hill cattle have also been used for dairy improvement as a dam breed for crossbreeding in some cases in the state. It has been observed that hill cattle in the state mostly reared as dual purpose animal i.e milk and draft. Therefore, the hill cattle could be improved for both milk and draft. The aim of the present study is to know the status, characterstics and performance  and to suggest the possible options for improvement in milk productivity of hill cattle in the state.

India’s livestock sector is one of the largest in the world and dairy farming is the single largest contributor to country’s GDP, apart from being the biggest source of employment. India has 56.7 per cent of world’s buffaloes. Buffalo genetic resource greatly helps the countries to assess their strengths and potential to harness the nature’s gift and India is fortunate in terms of having largest buffalo population, huge buffalo germplasm diversity (13 recognized plus 14 distinct population groups) and the world renowned buffalo breeds: Murrah, Banni, Jaffrabadi and Nili-Ravi.

The prospects of Farm Animal Genetic Resources (FAnGR) are exceedingly uncertain. The FAnGR characterize the genetic makeup of the thousands of local breeds worldwide which are well-adapted for producing in harsh environments, and which for millennia have been essential in nourishing human communities. Currently, the genetic diversity in numerous livestock breeds across the world is threatened. Decades of consistent selection intended mainly for production increase has caused irreversible loss of genetic diversity (FAO, 2009).

Introduction India is a vast country comprised of different agro-ecological regions with diverse climate, natural vegetation resources, topography, possesses almost all domesticated animal species, landraces and crops with different production systems. Domestic animals provide a considerable amount of   food requirements for mankind. India ranks first in milk production with 112.5 million tonnes milk produced in 2010-11. As India is a tropical country, in summer the temperature hikes about 40-48°C in various parts of the country. Such a temperature is certainly not within the comfort range of animals and thus the animals respond to the stress imposed by temperature on them in various ways to combat the stress and to maintain the homeostasis.

Livestock is an important source of income for the rural poor and marginal farmers which enables earn income and food security using common resources.It is a best option to utilize such waste resources in proper way to earn income. About 65-69% of Indian population consisting of small and marginal farmers and land less laborers reared animal as supplementary occupation by feeding their animals agriculture by products. Indian agriculture is very difficult to sustain without dairy animals. Per capita availability of milk comes out to 281gm per day, which is below than the average of 300gm recommended by Nutrition Council of ICMR. Thus, there is a further need to increase the milk production of the country in view of minimizing difference between per capita availability and requirement. Milk production in India has increased from a modest 22 million tons during 1970-1971 to about 122 million tons in 2011—2012 (Deptt. of Animal Husbandry and Dairying).

Dairy buffalo production has been a tradition in Asian region and some other parts of the world where fresh  buffalo milk, dahi (cultured sour milk), ghee (butter oil) and yoghurt are very popular. In Italy, the dairy buffalo industry is flourishing; thanks to the popularity of buffalo mozzarella cheese. Because of the market for Mozzarella, buffalo farming is a profitable enterprise and is carried out in an organized manner with modern equipment.

Introduction In India 70 to 75 per cent of the indigenous cattle and buffaloes are designated as non-descript that is they do not come under any well defined breed. Among the animals of defined breed also, less than 5-10 per cent of the population can be considered as elite and used for the production of breeding stock. Average productivity of cross-bred and desi cows is 2141 kg/lactation & 634 kg /lactation, respectively, which is still very low. The average productivity of buffaloes is 1355 kg/lactation only.

Breeds are the result of the hundreds of years of natural selection and evolution. They are adapted to tropical climate and diseases and perform reasonably well even with low quality of feed and fodder. India has agrarian economy, with majority of population dependent on agriculture. India is a mega diversity hub of different livestock breeds. There are 10 buffalo breeds, 30 cattle breeds, 42 sheep breeds, 20 goat breeds, and 18 poultry breeds contributing significantly to GDP of the country. Despite our vast population of different species, there is a need for conservation of breeds. Conservation is the management of human use of the biosphere, so that it may yield the maximum sustainable benefits to the present generation, as well as maintaining its potential to meet the needs and aspirations of future generation.

Introduction Over decades, improvement of domestic animal species has been done by selecting superior animals, mainly based on individual performance and their subsequent breeding to get progenies with higher production potential. Within the 20th century, understanding of inheritance of economic traits, factors influencing genetic potential and their interactions provided better criteria for selection and this has resulted into faster genetic gains. Several selection criteria which were composite ones based on the individuals as well as its relative performances were being used.

Bioinformatics is a branch of biological science which deals with the study of methods for storing, retrieving and analyzing biological data, such as nucleic acid (DNA/RNA) and protein sequence, structure, function, pathways and genetic interactions. It generates new knowledge that is useful in such fields as drug design and development of new software tools to create that knowledge. Bioinformatics also deals with algorithms, databases and information systems, web technologies, artificial intelligence and soft computing, information and computation theory, structural biology, software engineering, data mining, image processing, modeling and simulation, discrete mathematics, control and system theory, circuit theory, and statistics.

Introduction Agricultural biodiversity must be an integrated part of climate change adaptation and mitigation efforts. Climate change adaptation, biodiversity conservation and poverty alleviation should complement each other (FAO, 2007, 2008). Climate affects vectors, pathogens, hosts and host-pathogen interactions from the level of cellular defence to that of the habitat. Hoberg et al. (2008) provide an overview of predicted responses of complex host-pathogen systems to climate change. Climate change may affect the spatial distribution of disease outbreaks, and their timing and intensity (Epstein 2001). Outbreaks of African horse sickness, peste des petits ruminants, Rift Valley fever, bluetongue virus, facial eczema and anthrax are triggered by specific weather conditions and changes in seasonal rainfall profiles.

Poultry industry in India has a strong standing globally, being third highest egg producer with more than 50000 million eggs and fifth highest broiler producer with 2.25 million tones of broiler meat. Largely, Indian poultry industry is chicken dominated and the rearing of high yielding varieties under intensive rearing system is the backbone of its present face. Analysis of trend of egg production in last five years i.e. 2001-2006 revealed that annual growth rate in egg production is about 5-6 %, except a much lower rate of 3 % during 2003.

Poultry has been extensively used for various biological experiments and its contributions to science are impressive. Among poultry species, chickens have made fundamental contributions to our current understanding of many immunological processes and it ranks high in the biological world. It still serves as an animal model to investigate immunogenetic aspects of disease resistance and tumor regression. Among the major contribution from poultry, the first evidence for viral induced tumor came from chicken (Rous, 1911; Nobel prize, 1966), reverse transcriptase enzyme was isolated from Avian Leukosis virus (Tamin and Baltimore, 1963; Nobel Prize, 1975), oncogenes in virus are from host cells (Bishop & Harold Varmus; Nobel Prize, 1989) and major two arms of immune response i.e. the T and B cells were first discovered in chicken.

Rural poultry production constitutes vital component of agricultural economy in India. The organized sector of poultry industry is contributing nearly 70% of the total output and the rest 30% by the unorganized sector. In unorganised sector, poultry are reared in free range extensive system with very little input in the form of grain or farm by-products, birds have to scavenge from limited amount of feed resource. The productive out put of these birds are very low (60-70 eggs per bird per annum; 2.0 kg. and 1.5 kg. body weight per bird in males and females, respectively). Moreover, still more than 50 % population of poultry in India comprises of non-descript / deshi birds.

India, the progenitor of the present day chicken, is a vast country having varying climatic zones such as the high altitude Himalayan regions, the Indo-Gangatic plains, the Deccan plateau in the south and hot and humid coastal areas. Although, no exact data is available, factual information suggest that poultry raising in this sub continent is as old as its civilization. Owing to the long widespread chicken raising in the vast territory of this country and manifold ecological niches at different places, many native breeds were evolved over the years some of the documented breeds and ecotypes are Aseel, Ankaleshwar, Busra, Brown Desi, Chittagong, Daothigir, Denki,  Ghagus,  Haringhatta black, Kadakanath,  Kalasthi, Kashamir faverolla,  Miri,  Naked neck, Frizzle, Punjab brown, Tellichery, Titri, Teni and Nicobari.

Camels across the world are reared for milk, meat, draught, race and tourism. They are distributed in the African, Arabian and south Asian countries.  Apart from this, a feral population exists in Australia and recent reports indicate that a few animals of the species are also being reared in Europe. The world camel population shows an increasing trend with 12.92 m in 1961 to  24.68 m in 2010 producing 0.38 m tonnes of meat and 2.36 m tonnes of milk per annum.  The top 20 camel rearing countries produce both milk and meat with the exception of India and Pakistan as per FAO. Contrary to this, the research articles and news reports indicate that the species in the two south Asian countries too produce both milk and meat.

Biodiversity, coined by W. G. Rosen in 1985, is the variety of life on Earth. It is the occurrence of different kinds of organisms and the complete range of varieties adapted to different climates, environments and areas being constituents of food chains and food web of biotic interrelationship. Biodiversity refers to totality of genes species and ecosystems of a region. Biodiversity differs from place to place. Biodiversity is the biological wealth of world. There is continuous depletion of biodiversity due to increasing population resources consumption, urbanization and pollution. The basic reason for concern is that biodiversity is being lost even before it attains its size. Loss of biodiversity would check evolutionary capability of biota to cope up with environmental loss.

Livestock sector may be considered as a driving force for nutritional security and an important component of sustainable agriculture in India. Livestock sector besides providing milk and meat also provides a diverse range of output for agriculture (manure, draught power and transport) and for manufacturing sector (fiber and leather). According to estimates of the Central Statistics Office (CSO), the value of output from livestock sector at current prices was about 4,59,051 crore during 2011-12 which is about 24.8% of the value of output from total agricultural and allied sector at current price and 25.6% at constant prices (2004-05). The value of output of milk is 3,05,484 crore in 2011-12, which is higher than the value of output of paddy and wheat. The value of output from eggs and wool group is 17,803 crore and 318 crore respectively for 2011-12. Livestock agriculture is the only way to produce food in many of the world’s harshest environments. Locally adapted breeds enable these vast areas to be used in a sustainable manner.

India has vast animal genetic resources with a wide variety of indigenous farm animal breeds. These breeds have evolved over generations to adapt to specific agro-climatic and socio-economic needs of the people. Many number of these breeds are now subjected to fast genetic degradation and dilution because of unplanned and indiscriminate breeding and introduction of exotic germplasm. Domestic animal diversity in developing countries is embedded in traditional farming and pastoral communities who manage their livestock according to their Indigenous Technological Knowledge (ITK) and in tune with local ecological constraints.

Stress is described as the cumulative detrimental effect of a variety of factors on the health and performance of living beings (Rosales, 1994). Heat stress arises when there is an imbalance between heat production within the body and its dissipation. Homeotherms can maintain their body temperature within a relatively narrow range and both physiological and behavioural responses help in thermoregulation. Stress occurs when an animal suddenly faces a change in its environment. Heat stress during summer and post summer months in tropical countries is a problem of great concern among farmers and livestock producers as it costs both production and reproduction of animals.