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About The Author

H. P. PUTTARAJU
H. P. PUTTARAJU Professor, Sericulture/Life Sciences and Co-ordinator Biological Sciences (Integrated M.Sc.) Bangalore University, Bangalore, Karnataka

B. M. PRAKASH
B. M. PRAKASH Department of Sericulture/Life Sciences Bangalore University, Bangalore, Karnataka

B. C. KESHAVA MURTHY
B. C. KESHAVA MURTHY Department of Genetics and Plant Breeding University of Agricultural Sciences GKVK, Bangalore, Karnataka

About The Book

In this century, students of biology are confronted with an entirely different scenario. All aspect of biology become more molecular-molecular biology. The tools have transformed our information management, taking access information to new heights. The advances made by the molecular biology tools have been very phenomenal in understanding and solving many of age old problems involved with many plant and animal genomes. These tools have been very dynamic when combined with traditional paths of research to know the structure and functions of millions of genes. The present book chapters contain first hands-on information on methods and protocols in a simplified manner which is very easy to learn and perform. Further, methods and protocols constitute a gold-standard reference for today's scientists who wish to develop and hone their molecular biology skills towards the discovery of new biological relationships. This book has been divided into 10 chapters with each chapter containing introduction, principle, protocol, applications and troubleshooting and it has been written keeping in mind the requirements of graduate/postgraduate students and research scholars

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  1. Start Pages

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  2. Laboratory Equipments and Its Usage

    1 - 8 (8 Pages)
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  3. Buffers

    9 - 14 (6 Pages)
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  4. Cell Culture

    15 - 46 (32 Pages)
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  5. Nucleic Acid Extraction

    47 - 64 (18 Pages)
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  6. Determination of Quality and Quantity of Nucleic Acids

    65 - 70 (6 Pages)
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  7. Polymerase Chain Reaction

    71 - 84 (14 Pages)
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  8. Electrophoresis

    85 - 100 (16 Pages)
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  9. Restriction Digestion of Enzymes

    101 - 108 (8 Pages)
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  10. Blotting

    109 - 132 (24 Pages)
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  11. 10 

    Cloning

    133 - 144 (12 Pages)
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  12. 11 

    Sequencing of Nucleic Acids

    145 - 158 (14 Pages)
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  13. 12 

    End Pages

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