Preface Fruits and vegetables represent highly perishable items of agriculture produce, because of their high water content, chemical composition and physiology. Senescence sets in soon after harvesting and the fruits require to be subjected to scientific handling and storage or processed, to avoid the losses. The post-harvest losses in our country are estimated to be as high as 25% and this figure to reduce, needs scientific efforts at every stage of handling of the fresh produce, starting from the field to consumer. Proper stage of harvesting, grading, packaging, transportation assumes great importance to avoid post-harvest losses. Primary processing is proposed to convert the produce at the field level into pulp / juice / puree, that can be transported to centres for final processing into products. Thus, proper handling of fruits and vegetables is farmer’s economy, nutrition of the consumers and while doing so, the food safety assumes equal importance. Knowledge on the basic sciences of post-harvest handling of fruits and vegetables as well as processing into products is increasing year by year, on different commodities, in different countries, in different angles. Periodical reviewing of the subjects are vital to the academicians, students, industrialists and law makers too. Accordingly, this text book is formulated to provide the readers the benefit of recent collection of enmassed research, dealt subject-wise. A number of chapters on the basic and applied aspects are compiled to justify the title. We do hope the objective stands fulfilled to the readers.

1. Introduction Fruits and vegetables represent highly perishable items of agriculture, mainly because of their high water content in fresh form. Their production, yield and management under agriculture is dealt essentially under horticulture. They abound in species and varieties in different geographic regions of the world, influenced by the agro-climatic conditions. Some are endemic and confined to certain geographic regions only. Accordingly, they may be tropical, sub-tropical or temperate, depending on their requirements of temperature for growth and yield.

1. Introduction Vegetables and fruits are the next in demand for human consumption after rice and wheat, in all parts of the globe. Fresh fruits & vegetables and their value added products are increasing in popularity day by day almost in all the countries, because of their richness in dietary fibre, minerals & vitamin contents and for their medicinal properties. Maintaining agricultural produce quality and its evaluation are thus naturally important. Decisions concerning maturity and ripeness of fruits and vegetables are based mostly on visual inspections, which are subjective in nature. The quality of fruits and vegetables is mostly decided on the basis of size, shape, colour, gloss, flavour, firmness, texture, taste and freedom from external as well as internal defects. Internal quality factors such as maturity, sugar content, acidity, oil content and internal defects, however, are difficult to evaluate non-destructively. Methods are needed to better predict the internal quality of fruits and vegetables without their destruction. Recently, there has been an increasing interest in non-destructive methods of quality evaluation, and a considerable amount of effort is being made in this direction. But the real problem is how these methods are to be exploited practically on commercial scales and what are the constraints faced in implementing them.

1. Introduction Food is processed for its preservation, hygienization or value addition. To date, the food preservation technologies such as canning, dehydration, freezing, pasteurization and controlled atmosphere and modified atmosphere packaging have been the main processes employed to preserve foods, with the use of antimicrobials and microwaves also being employed more recently. In future, however, technologies such as exposure to ionizing radiation, high pressure processing, ohmic heating and pulsed electric field processing, could potentially be the new alternatives to the above food preservation technologies. Irradiation of food involves the exposing of food, either in prepacked form or as such to controlled doses of ionizing radiation being generated either by electrically driven machines such as electron beam accelerators, x-rays or gamma rays produced by radioactive isotopes such as CS-137,CO-60, to achieve certain desirable objectives. The process of exposing a range of food stuffs to specific doses of ionizing radiation is being used to extend the shelf life of food stuffs, to improve their overall safety and quality by eliminating pathogenic bacteria, by reducing number of food spoilage organisms, to reduce the bacterial load on herbs and spices and thereby making them safe to incorporate into food stuffs, to inhibit sprouting in stored tuber and bulb crops such as potatoes, onions and garlic, to disinfest grains, grain products and tropical fruits to prevent damage to the commodity and to meet the quarantine regulations(Arun Sharma, 2004, Farkas, 2006).

1. Introduction Pectin is a polysaccharide present in the middle lamella of plant cell walls. The firmness and structure of the plant tissue are attributed to the presence of pectin in the middle lamella. The insoluble form of pectin is called protopectin and the soluble form is called soluble pectin (Kertez, 1951). Protopectin is abundant in green fruits and contributes to hardness of cell, where as soluble pectin is responsible for viscous nature of juice and pulp. Conversion of protopectin to soluble pectin results in loosening of the bonds between the cells and softening of the fruit tissues. Protopectin is reported to be hydrolyzed to pectin by enzyme action during ripening. Protopectin on restricted hydrolysis yields pectin or pectinic acid. Braconnot (1825) discovered pectin as the gelatinous principle of fruits.

1. Introduction At present there is an increasing demand for colourants from natural sources. Since there is a possibility of banning of synthetic colours in view of their carcinogenic properties and already a reduction in number of permitted synthetic colours from 13 to 8 numbers in our legislation (PFA, 2005), the demand for natural colours is evident. In Japan and European countries because of the green movement, the demand for synthetic colours has been reduced. Synthetic colours in global market account for 400 million dollars. Despite processing limitations and higher costs, the global market for natural colours is estimated to be more than 939 million dollars (Espszezola, 1998). Color is recognized as a major factor affecting food acceptance. The consumer expects a specific food to possess a well defined colour and rejects any appreciable deviation from the normal. The change in colour during the preparation of food is a useful guide to quality control and is used by many food processors as the criterion for selecting raw materials. The characteristic colour of raw food is due to the natural pigments present in the plant and animal material. These can be enhanced in processed food products through the addition of food colourants. Addition of colour can make up for the colour losses during processing. Some of the major groups of colours are anthocyanins, carotenoids, betalains, chlorophylls, flavones and chalcones. Some of the plant sources of pigments are kokum, roscelle, chilli, marigold, beetroot, grapes and safflower. Sources of natural colours can be plants, animals or microbes. Fruits and vegetables form one of the main plant sources of pigments. Some of the plant materials rich in colours viz., alfalfa, marigold, safflower, strawberry, aronia fruits, grapes, red cherries have been exploited for pigment extraction and the results have shown positive effects. Some of the interesting features of the work carried out by researchers in this area are presented in the following sections.

1. Introduction In this era of genomics, proteomics and metabolomics the challenge in nutritional science is to clarify human nutrient requirements and design nutritional strategies to promote health and functional longevity. Our ancient Indian belief that ‘we are what we ate’ and our future health as individuals may well depend upon what we eat today. Plant-based dietary antioxidants are believed to have an important role in the maintenance of human health because our endogenous antioxidants provide insufficient protection against the constant and unavoidable challenge of reactive oxygen species (ROS). We have various endogenous defense chemicals but they are inadequate to prevent oxidative damage that makes human system prone to various degenerative diseases. Hence, it is necessary for increased intake of dietary nutraceuticals that may help maintain the balance between oxidants and antioxidants, slow age-related changes, and prevent onset of degenerative diseases.

1. Introduction Pulpy and juicy fruits like orange, guava, banana, grape, mango, papaya, pears, apples and jackfruit can be converted into fruit products like squashes, juices, cordials and ready-to-serve (RTS) fruit beverages. These RTS fruit beverages are both refreshing and thirst quenching and have become very popular. Liquid fruits are the clarified juices prepared from these pulpy and juicy fruits. There is a demand for liquid fruits in the international market for various applications. The liquid fruits can be used as a natural alternative to synthetic beverages. They can be suitably concentrated or diluted, blended with other juices and also can be carbonated as fruit based soft drinks. Removals of pulpy material from the juices make them easier to preserve by pasteurization and are amenable to carbonation. Pulpy fruits can be successfully processed into liquid fruits (clarified juices) by using pectic enzymes. The emphasis of the fruit juice processing industry has, so far, been mainly on conventional clarification processes like enzymatic and chemical treatments followed by filtration using filter aids; and concentration by vacuum evaporation.

1. Introduction Intermediate moisture foods are semi moist foods that have some of their water bound by glycerol, sorbitol, salt or certain organic acids, thus preventing the growth of many microorganisms (Stewart et al. 2001). It is now well recognized that fruits and vegetables are dehydrated to an intermediate moisture level (20-50%) (Stewart et al. 2001; Purvi et al. 2003). As the intermediate moisture products are characterized by a semi moist consistency so these foods have enough moisture content to permit easy chewing but low enough water content to prevent spoilage production of intermediate moisture foods is based on an increased scientific understanding of the chemical reactions involved in traditional food preservation methods (Purvi et al. 2003). There will be better retention of original flavor and texture compared to fully dried products, with concomitant reduction in bulk, weight and cost of packaging, transportation and storage. Such intermediate moisture foods are more appropriate for developing countries in view of their minimal processing requirements, stability under ambient conditions, safety, convenience, ease of nutrient content adjustment, energy savings and low capital investment. They are also eminently more suited than dehydrated or canned foods for military rations (FAO, 2005).

1. Introduction There is intense commercial interest in dehydrated fruit and vegetable products especially mango powder, banana powder, orange powder, papaya cereal flakes to powder, tomato juice powder and other vegetable powders, which are in great demand for soups, biscuit type cookies supplemented with fruit powders (Ana Maria et al. 2009; Sunita and Chauhan 2008), pizza mixes and sauces. Natural fruit and vegetable powders are gaining lot of popularity in recent years as they are very easy to handle after production, require minimum storage space and they are attractive. Tamarind powder is developed from tamarind fruit (Tamarindus indica) (Manjunath et al. 1991) and recently cashew apple and guava residues from fruit juice industry were successfully converted into fruit powders and used at different levels of wheat flour substitution for cookies formulation (Ana Maria et al.2009) and fruit puree like papaya can be mixed with cereal flour to prepare slurry and after gelatinization, the slurry can be useful for preparing drum dried flakes and powder (Sunita and Chauhan 2008). Tropical fruits are exotic in flavor but their juices and purees are seldom available in dehydrated powder (James, 1971). Citrus juice concentrates were produced during world war II in powdered form by using spray, drum, vacuum-puff, and freeze drying (USDA, 1962). The preservation of fruits and vegetables by dehydration offers a unique method, which may be considered as an alternative low cost preservation technique. A shift from fresh to more and more processed foods with significant value addition is bound to occur sooner or later considering the socio-economic changes taking place in the country and the rapid integration of Indian economy with global system. Most popular brands are found in the “ready to consume” food sector.

1. Introduction Bamboo has historically been an integral part of human culture. Bamboo is a mystical plant representing symbol of strength, flexibility, tenacity, endurance and compromise. Throughout Asia, bamboo has for centuries been integral to religions, ceremonies, art, music and daily life. Bamboo provides numerous environmental benefits such as playing a critical role in the balance of oxygen/carbon dioxide in the atmosphere. Bamboo is the fastest growing canopy for the regreening of degraded areas and generates more oxygen than equivalent ultraviolet rays. It is an atmospheric and soil purifier. Its soil conservation properties create an effective watershed, stitching the soil together along fragile river banks, deforested areas, and in places prone to earthquakes and land slides. Its fast growing and high yield nature makes it a highly valued renewable resource. Its low cost and abundance call for its utilization to a full extent. 

1. Introduction  Mushrooms have been part of man’s diet for centuries (Rajarathnam and Shashirekha, 2003). Mushrooms grow on wide range of agro-residues, biodegrade and biotransform them into edible fruiting bodies (Rajarathnam and Zakia Bano, 1988a; 1988b; 1990; Zakia Bano and Rajarathnam, 1988; Zakia Bano et al., 1993; Rajarathnam et al., 1998). Their post-harvest life is very ephemeral at room temperature (Rajarathnam et al., 1983) due to delicate flavor (Rajarathnam et al., 1990) and high moisture content, with a range of chemical constituents (Zakia Bano and Rajarathnam, 1986; 1982; 1988; Rajarathnam and Zakia Bano, 1990; Rajarathnam et al., 1983; 1992). Since, they contain large quantities of water and therefore suffer considerable weight loss during transportation and storage, causes serious economic losses, due to reductions in weight and quality (Gray, 1970). When the supply of water, organic substances and minerals are cut off at harvest, the fresh mushroom enters a deterioration or perishable phase. Cap opening and stem elongation are the usual symptoms of senescence constituting visible evidence of deterioration. At room temperature, the shelf-life of mushrooms cannot be for more than 24 h (Rajarathnam et al., 1983). During this period, there are considerable changes in color, texture and taste. In addition, water is continuously being lost as a result of transpiration and respiration. Additional loss occurs from surface cracks and bruises that develop during growth and handling (Salunkhe et al, 1991). Fresh mushrooms are white or light buff, with no dark marks on either the cap or stem. The veil is closed and the gills are not visible. The upper surface of the caps should be strongly convex and stems should be plump rather than elongated. Mushrooms are grown on compost, usually in mushroom houses where the temperature and humidity can be controlled. Eventually after harvest, the mushrooms can never be able to withstand any increase in temperature and decrease in relative humidity. Mushrooms are commercially preferred at the immature stage characterized by closed caps, invisible lamellae, round stipe and light color. Agaricus development is classified into various stages (Table 1). Thus, the concept of physiology of fresh mushrooms being very complicated, bears a great impact on the delicate problem of extending the storage life. The various means and methods studied and adopted by various researchers to aim at extended storage life of fresh produce, underlined by its physical, chemical and biochemical properties and also development of value added products are critically evaluated.

1. Introduction Fruits and vegetables in fresh form in general contain very high water content, with the fast rate of metabolic activity, including respiration rate, which may vary with the fruit type. Accordingly, the storage life at room temperature is considerably less, to expect acceptable quality. A range of transformational changes occur in various features of the fruits and vegetables, such as color, flavor, texture, structure, sweetness, sourness, pungency, astringency and so on. Phenolic browning of mushrooms, intensive softening of papaya, coloration and softening of ripe banana, softening of pear and jackfruit , flavor transformations of mango, banana and jackfruit after ripening, their color and texture transformations, are classic examples of post-harvest changes in fresh commodities, over a course of storage period at room temperature. These physical and sensorial perceptions of fresh commodities over a length of storage period are based on the range and multitude of operations of physical, chemical and bio-chemical reactions occurring in various constituents of the fresh fruit or vegetable such as moisture, pigments, vitamins, pectin, cellulose, hemicelluloses, phenolics, lignin, sugars, flavor compounds, amino acids, fatty acids and so on. In order to increase the storage stability, the fresh fruits and vegetables are processed and converted into various products such as juice, RTS beverages, jam, jelly, marmalade, fruit spread, dehydrated, spray dried powder, frozen stored, fruit cereal flakes, canned, candied, fruit bar and so on. Based on the nature of processing, the original form, shape, size, texture and structure are transformed, however with due maintenance in storage acceptability. A number of external factors are applied to achieve this product range such as heat, air velocity, pressure, radiation, chilling, freezing, vacuum impregnation etc. The ultimate quality of the final product is essentially an interaction between and amongst the various chemical constituents so characteristic of each fruit or vegetable under study, and a sum total of positive and negative reactions related to sensorial acceptability of the product. This quality is again a function of the storage period and the conditions of storage. Consumers in modern days are more health conscious to choose between consumption of fresh or processed fruits and vegetables. For trade over distance and time and particularly for geographic regions not producing a particular fruit or vegetable, it is of utmost importance to process the fresh produce. Enzymatic transformations in color, texture and flavor deserve utmost attention to ensure acceptable quality of the processed product, in particular over the course of storage period or until consumed after transportation and marketing. Microbiological quality of the product in final form is a most vital factor of consumers' health and this is dependent on water activity, sugars and protein / amino acid contents. In this regard, additives and preservatives come into picture. Strict regulations are imposed on their use, type and concentration over a storage period, for healthy consumption. Accordingly, this chapter would deal with several examples of processing conditions influencing the quality of the product, that emanates. Processing involving chemical, bio-chemical, engineering, bio-technological and microbiological methods to achieve a product are vivid, varied and different to different species / variety of fruits or vegetables to target different products.

1. Introduction Processed foods have become a way of life in the modern world. Manipulation of chemical and physical properties facilitated by modern food technology and followed by addition of food additives has created a plethora of innovative food products that offer consumers greater convenience, variety, safety, satiety and quality. Food additives have been in use for centuries for preservation, for providing improved appearance or desired texture and flavour. Preservation of foods started with salting and smoking. The Egyptians used colours and flavours, Romans used saltpeter and in India, turmeric is being used. Similarly additives such as baking powder as a raising agent, thickeners for sauces and gravies and colours- synthetic as well as from natural sources are being used. Today more than 2500 food additives have been in use mainly due to the spurt in the research activities in food science and technology that are aimed at providing different characteristic functions to foods. Out of these, about 1300 are the flavouring additives.

From the earliest time, man had to conserve and preserve his food, so that surplus at harvest following a successful crop can be stored for use throughout the year. Prime agricultural products in general and more so with fruits and vegetables which are being processed world wide or preserved to a more stable form so that they can withstand transport, handling and storage over a long period. As civilization developed, man learnt to preserve fruit and vegetable products with salt, spices and by drying , smoking or cooling which could extend their shelf life. Later by experience, he learnt that by protecting it from environmental factors such as air, moisture and light, the shelf life of these processed products could be further increased. He tried to keep them dry and covered to protect from insects and rodents. As with solid foods, primitive man experimented with beverages also. It is likely that the first beverage known to him after water was fruit juice. This undoubtedly led to the discovery of wines made from the fermented fruit juices. Later on, preservation methods and development of packaging materials underwent drastic changes.

1. Introduction Fruits and vegetables have been an integral part of man’s diet from immemorial times. Fruits are the portions of plants that bear seeds, while vegetables are the edible components of a plant, including the leaves, stalks, roots, tubers, bulbs, flowers and seeds. These can be eaten raw or cooked. Fruits and vegetables can be preserved for short-term intervals under refrigeration, with or without receiving a minimal treatment like slicing, chopping, shredding or peeling. Fresh produce may also be irradiated or packaged in controlled or modified atmosphere in films for short-term storage. Long-term preservation includes canning, juicing, drying and addition of preservatives. Food contamination with hazardous biological agents remains a worldwide challenge in food safety and nutrition (Kenny 2002; Schlundt 2002; Tauxe et al 1997). Microorganisms form part of the epiphytic flora of fruits and vegetables and many will be present at the time of consumption. Production practices, growth conditions and the location of the edible part during growth (soil, soil surface, aerial part) will in combination with intrinsic, extrinsic, harvesting and processing factors affect their microbial status at the time of consumption. Man-made sources may be directly from farm laborers and from the equipment used for harvesting or storage. The types of microorganisms on fruits and vegetables would also vary with the orchards, vineyards or growing fields. The resident microflora normally subsists on the slight traces of carbohydrates, proteins and inorganic salts that dissolve in the water exuding from or condensing on the epidermis of the host. The numbers of bacteria present will vary depending on seasonal and climatic variation and may range from 104 to 108 per gram. The inner tissues of fruits and vegetables are usually regarded as sterile (Lund 1992). However bacteria can be present in low numbers in the insides of the fruits and vegetables as a result of the uptake of water through certain irrigation or washing procedures.

1. Introduction The chemical composition of a food is of utmost importance from many standpoints, including nutrition, safety, and stability to microbiological, chemical or physical changes. Analysis of these food components is required to provide nutrition labelling for informing the health-conscious consumer, for quality assurance during processing and storage, and for regulatory purposes. The methodology used in analysis will depend on the particular component and the nature of product being analyzed, the purpose of the analysis, and the availability of resources. The emphasis of this chapter is on understanding the principles, procedures and calculations involved in estimating a particular component in fruits, vegetables and their products.

1. Introduction Mushrooms represent a highly specialized group of fungi, mostly basidiomycetes, with production of fruiting bodies bearing the spores for continued propogation. The fruiting bodies vary greatly in shape, size, color, texture, structure and aroma (Rajarathnam et al., 1998). They are endowed with the unique ability to biodegrade cellulosic wastes, including lignin, and biotransform into edible fruiting bodies (Rajarathnam et al., 1992, 1998, Rajarathnam and Zakia Bano, 1990). Their chemistry is also varied comprising characteristic polysaccharides, phenolics, proteins, amino acids, peptides, lectins, fatty acids, minerals, and so on (Rajarathnam et al., 1998). The wide spectrum of compounds have endowed the mushroom species with a spectrum of biofunctional activities of medicinal importance and accordingly their role in human health are implicated through studies on chemical, cell culture and animal feeding experiments. Vast literature available on this subject is condensed for presentation here. Fifty nine medicinal species are reported from Diaoluoshan Nature Reserve in Hainan Province, China (Li et al., 2003). A health food made from luminescent mushrooms (Mycena chlorophos) is described. The luminescent parts that emit light in the carpophore of the mushrooms are dried without thermal decomposition and pulverized (Sugimoto, 2006). Consideration is given to the health promoting properties of 3 types of Asian fungi, namely, shiitake (Lentinus edodes), reishi (Ganoderma lucidum) and maitake (Grifola frondosa). Properties considered include beneficial components (e.g polysaccharides, vitamins, amino acids) and effects on the immune system (Anon, 1997). Auricularia auricula (kikurage or tree-ear) and Tremella fuciformis (shirokikurage or white jelly-leaf) are widely used in Chinese cuisine. Chemical structures of some of the polysaccharides from A. auricula and T. fuciformis, in relation to their medicinal and nutritional effects are described (Misaki and Kakuta, 1995).

1. Introduction  Banana is the developing world’s fourth most important food crop (after rice, wheat and maize) accounting for 70 million metric tons per annum (FAO stat, 2007 faostat.fao.org/site/567/Desktop Default.aspx?). Banana is also the second highest fruit crop in the world, representing a wide range of varieties of cooking and dessert types, providing a staple, nutritious inexpensive food for millions of people. The word “banana” is a general term embracing a number of species or hybrids in the genus Musa of the family Musaceae known simply as the banana family. Two Musa species namely Musa acuminata and Musa balbisiana, out of the 28 described were first noted in 1865 by Kurz as being the origin of most of the commercial clones (Stover and Simmonds 1987). Cultivated clones are parthenocarpic, mostly sterile with starchy fruits. Indeed, wild types are very fertile; their fruits are full of seeds with little starch, and are seldom fit for human consumption. Its abundance, varietal diversity, availability round-the-year and low cost make banana an important commodity for the religious occasions in many tropical countries. The vegetable bananas, generally grouped under ‘Plantains’ are larger, more angular than dessert type and are generally intended for cooking unripe, although ripened ones are quite like dissert bananas.

1. Introduction As dealt in earlier chapters, fruits and vegetables represent the most essential of horticultural component for acreage of production, yield, nutritional and nutraceutical values, of definite taste, texture, flavor and colour, involving defined health and medicinal benefits. Every year and over the course of every ten years, significant quantum of research data accumulates on the various facets of fruit and vegetable technologies, and also trends in production, processing and marketing, may also tend to change. At every stage, more thinking has gone to improve the technology, product quality and cost economics to suit wider markets, both domestic and foreign. It is a continuous endeavor to record newer and newer problems emerging and to set the strategies to solve them. Several relevant references, fitting to the various aspects representing the cross section of the fruit and vegetable technologies are dealt here, to conclude the subject and spell out the future. Consumer demand for healthy convenience meals with ‘near fresh’ properties challenges researchers and industry to develop new or improved conservation procedures for processed products. Development of a dehydration process based on electromagnetic energy (EME) may bring about a major breakthrough with respect to retention of product quality and improved rehydration characteristics (Nijhuis et. al., 1996). Economic potential and current limitations of fruit and vegetable processing in India included improvements in preliminary treatment (harvesting, washing/cooling) precooling (including refrigerated transport/storage) and processing of juices and pulps. Establishment of a National Horticulture Board has aided to provide market information and so aid growers to get remunerative prices (Anon, 1993). The use of food irradiation as a quarantine method for treatment of food and agriculture commodities was organized by the joint Food and Agriculture Organization/International Atomic Energy Agency Division of Nuclear Techniques in Food and Agriculture, held in Kuala Lumpur, Malaysia, on 27-31 Aug., 1990. (FAO,1992). This has been experimented with various commodities, particularly for export trade of fruits like mango, as stipulated by the importing country like U.S.A.