Preface It is expected that about 85 per cent of the expected global population of 9.1 billion will be living in developing countries by the year 2050. However, the capacity of available natural resources and crop production technologies to satisfy the demands of this growing population for food and other agricultural commodities remains uncertain. Recently, concerns have been voiced that agriculture might, in the future, no longer be able to produce the food needed to sustain an ever-increasing world population at levels required to live a healthy and active life. The continuing decline of per capita arable land may be an indicator of impending problems. The underlying cause for such problems is perceived to be an ever increasing demand for agricultural products facing finite natural resources such as land, water and genetic potential. Scarcity of these resources would be compounded by competing demands for them originating in urbanization, industrial uses and use in bio-fuel production, by forces that would change their availability such as climate change and the need to preserve resources for future generations through environmentally responsible and sustainable use. Indian agriculture, in particular, has to meet formidable challenge, mainly by stagnant production on land already in use and by avoiding further encroachment on land that is only marginally suitable for cultivation combined with global warming posed climate alterations. Major adjustments are needed in agricultural, environmental and macroeconomic policy, at both national and international levels, in developed as well as developing countries, to create the conditions for sustainable agriculture. Also, a quantifiable amount of agricultural produce (grains, fruits and vegetables) is being wasted as post harvest losses, thus, again worsening the situation. However, conserving and rehabilitating the natural resources on marginal lands in order to maintain sustainable man/land ratios are necessary. The main tools are policy and agrarian reforms, participation, income diversification, land conservation and improved management of inputs such as new climate resilient varieties, integrated nutrients and disease management system coupled with intensive agriculture by diversifying the production systems for maximum efficiency in the utilization of local resources, while minimizing environmental and economic risks. There is a need to integrate sustainable development considerations with agricultural policy analysis and planning in all countries, particularly in developing countries like India, for improving farm production and farming systems through diversification of farm and non-farm employment and infrastructure development; water for sustainable food production and sustainable rural development; conservation and sustainable utilization of plant genetic resources; integrated pest management and control in agriculture and sustainable plant nutrition to increase food production. The absence of a coherent national policy framework for sustainable agriculture and rural development is widespread and is not limited to the some over exploited parts of the country. In particular the transitions from planned to market-oriented systems need such a framework to incorporate environmental considerations into economic activities, including agriculture. The major thrust of food security is to bring about a significant increase in agricultural production in a sustainable way and to achieve a substantial improvement in people’s entitlement to adequate food and culturally appropriate food supplies. Agriculture needs to be intensified to meet future demands for commodities and to avoid further expansion onto marginal lands and encroachment on fragile ecosystems. Increased use of external inputs and development of specialized production and farming systems tend to increase vulnerability to environmental stresses and market fluctuations. Plant genetic resources for agriculture are an essential resource to meet future needs for food. Special emphasis should be placed on the minor crops and other underutilized or non-utilized species of food and agriculture. There is genetic erosion of invaluable crop species. Attempts have been made to cover various aspects of modern agriculture viz. genetic improvement of crop plants, modern methods in plant breeding, seed science, ground water resources management, integrated farming systems, horticultural crops, biological control for sustainable agriculture, underutilized fruit plants health enhancing foods, role of enzymes in food processing, bioinformatics and molecular diagnostics etc., but still many facets lingering due to vast nature of agriculture itself .

Abstract The genetic improvement of crop plants is possible only through employing an appropriate breeding technique. The use of plant breeding technique depends on mode of pollination and reproduction of plant species e.g. selection and hybridization are used in sexually reproduced species, while clonal selection in asexually propagated plant species. The plant species/ varieties are usually derived through conventional techniques (vi z. introduction, selection, hybridization etc.), which are commonly used in various breeding programmes, while some modern techniques viz. transgenic breeding and marker assisted selection etc. are also available which are equally effective for improvement of all crop species. Various techniques applied for development of new varieties and hybrids are briefly described in this section. Plant breeding is an art, science and technology of improving genetic make-up of crop plants in relation to their economic usefulness for mankind. Various techniques/methods of breeding used for genetic improvement of crop plants are: introduction, selection, hybridization, mutation, transgenic breeding etc. Classification of Breeding Techniques: Plant breeding techniques are classified as conventional and modern breeding techniques.

Abstract Plantbreeding is an ancientart practiced throughselection ofplants fordesirable traits. Over a period of time it has evolved into scientific discipline with various methodologiesbasedon theories and principles ofgenetics. Since the rediscovery of Mendel’s law, there has been accelerated increase in yield of various crop plants due to development of high yielding varieties. Conventional approaches of following phenotypic selection from a variable population in early generation followed by yield testing in later generations have been highly successful in most of the crop species. This coupled with hybrid varieties led to significant increase in food-grain production during later half of the 20th century. At present manycrops are facing yield plateau asthey have reached their maximum genetic potential. The growing condition is also deteriorating due to changing climate. There is an urgent need among breeders to utilize full arsenal of techniques available to them to sustain crop productivity. Molecular markers and marker assisted selection has come as a boon to the breeders to increase the efficiency ofselection in early generation breeding especially for traits whose phenotypic evaluation is difficult. Further, transgenic technology has the potential to incorporate genes from diverse sources, thus enriching the gene pool from which breeders can search for specific traits. Although at present there is skepticism about transgenic crops among public but this may become a viable strategy in future.

Abstract Seed technology deals with the various aspects of seed- right from production to make it readily and timely available to the end users. The advancement in seedtechnology was realizedlong back and itwas considered equally important to the development of new technology (varieties). Considering the importance of seed as well as variability among seed of different crop species or even in same crop species, the researchers initiated work and focused their direction of research accordingly which resultedinto worthy achievements. Brief overviews of the advancements made in seed technology are being discussed in this chapter. However, efforts are still on to get rid of problems of seed technology in order to strengthen the seed industry, which makes the highest contribution to rural development, probably among all industries.

Abstract Water scarce state Rajasthan is without efficient and effective management. Water, when at surface is cheaper and like free commodity to use. Most of it gets evaporated rapidly due to aridity. There is no water during summers and drought years. This is now critical time to rethink and review our planning and policies and make it ground water oriented instead of completely surface water based. Ground water management should be the thrust area and key issue. There is need to conserve andharvest each drop ofwater especially for recharge to ground water. Utilize the augmented ground water resources as crisis management during long dry spells. Also, enforcement of regulatory measures, creating awareness among masses and participatory approach ofmanagement are the key factors for ensuring sustainable availability of water resources.

Abstract Modern agriculture largely depends on use of high cost inputs and energy intensive farm machinery. Worlds increasing population and continuously deteriorating soil fertility has alarmed agriculture system to look for some safe alternatives. Organic manures are rich source ofnutrients and humans, therefore crop residue recycling by composting is a best alternate of modern high cost input based agriculture. Compost is an aerobically decomposed organic matter .Bacteria, fungi, actinomycetes algae and protozoa are involved in the process of composting and are found throughout the natural environment. The composting material warms up due to microbial activity and it’s temperature becomes up to 160 0F active stage of microbial activity . There are four types of compositing i.e. Windrow composting, Aerated static pile composting, In-Vessel composting and Vermi-composting. The C: N ratio, aeration, temperature and moisture are major factors affecting composting process. Major advantages of composting are: Aggregate formation, Reduction in bulk density, Increase in hydraulic conductivity, Reduction in loss of soil moisture, Increase in organic carbon content and pH of soil.

Abstract Sustainable agriculture or farming is one that contributes to the overall objective ofsustainable developmenti.e. to meetthe present needs withoutcompromising the ability of the future generations to fulfill their own food needs and related demands from the land. A farming system is a complex, interrelated matrix of soils, plants, animals, power, implements, labour, capital and other inputs controlled by farming families and influenced to varying degrees by political, economical, institutional and social forces that operate at any levels. Research witha farmingsystems perspective has variousobjectivesranging fromincreasing the body of knowledge about farming system. The whole farming rather than the individual crops/enterprises need to be considered in the decision making under the farming systems approach. This is a multidisciplinary whole-farm approach and very effective in solving the problems of small and marginal farmers. Farming systems approach in analyzing the problems of agriculture is gaining lot of importance in recent years. Such a study would throw light on the problems associatedwith different farmingsystems and enable theacademicians and policy makers to formulate and implement appropriate policies for a balanced and integrated agricultural development.

Abstract Though India is second largest producer of fruits and vegetables but is far behind in per capita availability of this protective food as compared to many developed countries. Floriculture and allied horticulture crop production (spices, medicinal & aromatic crops, mushroom etc.) have great potential to generate high income, jobs and new careers. Hi-tech horticulture is the need of all the developing countries and it refers to “the precise production techniques for efficient use of inputs at appropriate time and quantity for maximization of yield and quality of crops”. Various ways to make horticulture hi-tech include modern nursery management including micro propagation, protected crop production, high density planting including canopy management, use of agrochemicals, rejuvenation technique, interated nutrient management, integrated water management, integrated pest and disease management. Inspite of this, in present context, major emphasis has been given on clean horticulture production with bio-derived, renewable energy sources for developing a profitable CEA (controlled environment agriculture) that would help in sustaining the system, improving socio-economic status of growers and of-course environment-friendly.

Abstract Indian is rich biodiversity and availability of a variety plants of enhances the interest of scientist for the possible use of plant products as natural herbicides. The emphasis should be to collect the information about such plants from the concerned people who have traditional knowledge about the use of these herbs and then to study scientifically to arrive at conclusions. The study of compounds produced by a plant which inhibit or stimulate the germination and the development of other plants is important for understanding the mechanisms of the ecologicalinteraction. Therefore, there is continuing need to develop and release new phytotoxic chemicals that preserve the environment and enable a more efficient control of weeds, improving crop yield and quality. Ageratum conyzoides a herb traditionally used for treatment of many diseases, releases some potential allelochemicals and other chemicals to the environment which inhibit the germination and growth of neighboring plants. A complete study of its potential allelochemicals may be useful in biological control for sustainable agriculture.

Abstract The conventional methods of pathogen identification have often depended on identification of disease symptoms, isolation and culturing of the organisms, and identification by morphology and biochemical tests. The major limitations of these culture based morphological approaches, however, are the reliance on the ability of the organism to be cultured, the time consuming nature and requirement of extensive taxonomic expertise. Furthermore, diagnosis of plant diseases can be even more difficult with asymptomatically infected propagative materials such as tree grafting stocks or potato tubers. The use of molecular methods can circumvent many of these shortcomings. Accordingly, there have been significant developments in the area of molecular detection of plant pathogens in the last three decades. The polymerase chain reaction (PCR) which revolutionised molecular diagnostics and biological sciences. In the last decade the range of targets that can be diagnosed using diagnostic PCR have grown tremendously. The very flexibility and application specific variations in the basic theme of the system have allowed the development of many PCR variants adapted to wide range of applications. Furthermore, diagnostic PCR has been greatly improved by the introduction of the second generation PCR, known as the Real time PCR where closed-tube fluorescence detection and quantification during PCR amplification (in real time) is possible eliminating the need for laborious post-PCR sample processing steps which greatly reduces the risk of carryover contamination. Using Real Time PCR, it is possible not only to detect the presence or absence ofthe target pathogen, but it is also possible to quantify the amount present in the sample allowing the quantitative assessment of the number of the pathogen in the sample. Enumerating the pathogen upon detection is crucial to estimate the potential risks with respect to disease development and provides a useful basis for diseases management decisions. Crops can be attacked by many pathogens which, in addition, often occur in complexes. Therefore, many disease diagnostic applications require simultaneous detection and quantification of several targets. Methodological limitations, however, are in many cases the reason for developing simplex or assays designed for only few targets. The DNA Microarray technology, originally designed to study gene expression and generate single nucleotide polymorphism (SNP) profiles, is currently a new and emerging pathogen diagnostic technology, which in theory, offers a platform for unlimited multiplexing capability. It is viewed as a technology that fundamentally alters molecular diagnostics. The fast growing databases generated by genomics and biosystematics research provides unique opportunity for the design of more versatile, high-throughput, sensitive and specific molecular assays which will address the major limitations of the current technologies and benefit plant pathology.

Abstract Ber is an important fruit of India and South Asia. Its cultivation is gaining popular in recent years. The drupaceous fruits of ber are characterized by climacteric respiration phenomena and produce high amount of ethylene at ripening. It is very high in ascorbic acid content. Maturity and ripening is still judged by colour changes, as reliable maturity indices for particular cultivars are not fully established. Short shelf life is major problem in this fruit. Protocols for postharvest handling is not standardized, however, scattered information are available for postharvest behaviour and handling. Low temperature storage is beneficial and reduces respiration rates but simultaneously chilling injury is again hindrance in commercialization. Postharvest rot is major problem in storage. Therefore, focused research is needed to standardize the storage temperature and alleviation of chilling injury.

Abstract Molecular markers play key role in crop improvement. There are severalmarkers such as RAPD, SSR, AFLP, SNP, DArT and many more are available for their practical uses. From the past markers like RAPD, AFLP, and SSR have been used extensively in the crop breeding. Recently new markers such as SNP and DArT have become popular among the researchers of molecular biology for use in genomics-assisted crop breeding. These markers have been applied for study of genetic diversity, germplasm management, cultivar identification, genetic mapping, gene tagging and genomics etc. Though the conventional method of breeding willcontinue to play a primary role in the crop improvement, molecular marker technology is becoming one of its integral components. Agarose-based DNA markers like ISSR, CAPS, RAPD, SCAR and AS (allele specific) have the potential for faster and accurate breeding for target traits through MAS.

Abstract Health-conscious consumers are increasingly seeking functional foods in an effort to control their own health and well-being. The field of functional foods, however, is in its infancy. Claims about health benefits of functional foods must be based on sound scientific criteria. A number of factors complicate the establishment of a strong scientific foundation. These factors include the complexity of the food substance, effects on the food, compensatory metabolic changes that may occur with dietary changes, and, lack of surrogate markers of disease development. Additional research is necessary to substantiate the potential health benefits of those foods for which the diet-health relationships are not sufficiently scientifically validated. Research into functional foods will not advance public health unless the benefits of the foods are effectively communicated to the consumer. Finally, those foods whose health benefits are supported by sufficient scientific substantiation have the potential to be an increasingly important component of a healthy lifestyle and to be beneficial to the public and the food industry. Mounting evidence supports the observation that functional foods containing physiologically-active components, either from plant or animal sources, may enhance health. It should be stressed, however, that functional foods are not a magic bullet or universal panacea for poor health habits. There are no “good” or “bad” foods, but there are good or bad diets. Emphasis must be placed on over-all dietary pattern, which follows the current Dietary Guidelines, and is plant-based, high in fiber, low in animal fat, and contains 5-9 servings of fruits and vegetables per day.

Abstract Fertilizer inputs are increasing the cost of production in inputintensive crops, so nutrient efficientcultivar is need oftoday. More demands for food by increasing world populations further enhance the importance of nutrient efficient crop cultivars that are higher producers. Breedingtechniques havebeenimplemented significantlyto breed highnutrientefficient crop cultivars to achieve the targets. However, molecular mechanism of nutrient metabolism has been understood to some extent in many crops through quantitative genetic approaches. This minireview outlines an update on recent progress on the genetic approaches of QTL/candidate genes controlling nutrient metabolism pathways in the crop plants.

Abstract Bioinformatics is a field of science which studies biology, computer science, and information technology. The ultimate goal is to obtain useful information by data acquisition, analysis and interpretation of sequences data. In the current decade, a huge volume of sequence data is stored in the data bank due to global genome initiatives of many organisms. Management and understanding of the genome data cannot be possible without the bioinformatics tools. A large number of tools and softwares packages are available either commercial or freely online for the bioinformatics applications. The use of bioinformatics tools enhances the research capability for crop improvement through genome sequencing, database management, assembly, alignment, primer designing, markers development, genes prediction and annotation, gene expression, phylogenetics, protein structure identification, metabolic pathways analysis and many other applications.

Abstract Issues regarding intellectual property rights and their protection are heralded nowadays in the scientific arena both in developed and developing countries. IPR is being given greater attention and importance due to the projected idea that it is a dependable means through which greater skills and knowledge can be utilised to serve as a reliable resource for the upliftment of the economy as well as knowledge dissemination. In context of microorganisms, India does not allow patenting of microbes whichalready exists in nature assuch but genetically modified version of the same microorganism with enhanced and improved efficacy can be patented. The deposition of microorganisms in a culture collection recognized as an international depository authority (IDA) is necessary if intellectual property rights regarding these microbes are to be claimed. An action plan for developing countries towards creating IDAs and preserving the natural gene pool has been evolved. It is likely that IDAs will transform into ‘biological resource centres’ and further into‘global common genetic resources’, with an internationally agreed legal basis for benefit sharing. This chapter is an effort to highlight the genesis of patenting and legalities of patenting microorganism in India.

Abstract Potato in India was introduced as a long day temperate crop which found suitable climate in the higher hills of the country. Since then it is grown widely in the Indo-Gangetic Plains, the Plateau and the Himalayan regions in north and the Nilgiris hills in south of India. The Central Potato Research Institute (CPRI), Shimla has developed 49 potato varieties by the conventional breeding for suitable cultivation of potato in different potato zones of India. A number of technologies for ware- and quality seed- potato production, storage and processing have been developed/standardized for adoption by the end users. These technologies have contributed immensely towards multiple increase in area, production and productivity of potato during last sixty years. Apart from conventional tools, modern biotechnological tools like whole genome sequencing technology, transgenics technology, molecular markers, RNAi and microarray technology have are also being employed in potato improvement.

Abstract In the present horticultural scenario of India, this is the burning issue as how best the underutilized horticultural crops can be exploited. There is tremendous potential for these crops especially for diversification. These plants can generate employment for millions especially to the natives ofdeveloping counties besides nutritional security. Traditional wisdom of Indian farmers if used properly can pave the path for transforming the economy of developing nations. There are great opportunities for the people to identify crops, explore their market, standardize their cultivation and processing techniques, document them and provide jobs to millions. This will salvage the people from exploitation by multinational companies of developed nations and can give a new direction to sustainable agriculture.

Abstract Genomics is playing an increasing role in generating new tools and databases with the rapid advances in genome technology which increases the eficiency and precision of crop improvement. With the emergence of new high-throughput technologies like next-generation sequencing (NGS) and reduction in their costs are bringing many more plants within the range of genomic and post genomic data along with the integration of information from the related fields of transcriptomics, proteomics, metabolomics and phenomics. Translating the vast abundance data produced by genome technologies requires large generic databases which hold specific data types for a broad range of species, which act as a resource for the crop improvement. The development of custom bioinformatics tools and advanced databases has become an expanding challenge which systematically helps in storing, organizing, manipulating and analyzing large amounts of biological information computationally. In this review, we outline some recent advances in the features of plant genome databases and various platforms with the recent advances in technology.

Abstract The applications of enzymes are awe inspiring marvels of nature. Scientists can use enzymes to explore all aspects of biology; from molecules to cells to tissues to organs to organism as a whole. For the purpose of food and drinks, enzymes have been exploited by men for thousands of years without really knowing what they are and how do they work? Enzymes have played a pivotal role in many aspects of life since the dawn of life. Today, biocatalysis is being used as a useful alternative to chemical process technology. The fact that enzymes work under much milder conditions oftemperature, pressure and acidity, thus, obviate harsh processing conditions and thereby decrease the possibility of damage to heat labile substrates and save energy. Enzyme technology being eco-friendly can be justifiably termed as‘Green technology’. Due to their high specificity and high catalytic rate, enzymes prevent wastage and reduce time of processing / manufacturing, respectively. The ability of enzymes to act on wide variety of substrates has made them useful tools to exploit cheaper starting material in food industry. Reportedly, over 45% of the enzymes produced are being used in food industry and remaining is shared by detergent (34.4%), textile (11%), leather (2.8%) paper and pulp (1.2%) and other industries (5.6%). Enzymes serve a myriad of functions in food processing, from maximizing juice extraction of pectin-rich fruits to extending shelf-life in baked goods through slowing starch retro-gradation. Indian food processing industry has seen significant growth and changes over the past few years. Though, the market of enzymes is increasing at a fast pace but most of the enzymes of the food sector are coming from overseas. Seeing the unparallel role of enzymes in food processing sector as in multifaceted domains, the researchers should emphasize upon finding novel enzymes and explore their untapped commercial benefits using modern techniques of enzyme engineering, immobilization and biotechnological interventions.