Preface Horticulture plays an important part in today’s agriculture and there are new avenues that are being achieved by horticulture. The subject has transformed from only being about vegetables, fruits, flowers and postharvest technology and has moved towards disease, breeding, pathology, physiology, greenhouse technologies and other areas which were never heard for. The book series: Hi-Tech Horticulture has been worked out keeping the above mentioned issues in mind with contribution by eminent professors and scientists. The volumes are on: 1. Crop Improvement Nursery and Rootstock Management 2. Organic Farming and Sustainable Development Techniques 3. Improved Production Techniques 4. Plant Protection and Stress Management 5. Value Addition and Post-Harvest Management 6. Advance Techniques

Abstract Modern agriculture has disturbed the age-old balance of host-pathogen system resulting diseases occur at regular interval leading to enormous loss to the agricultural production, which is estimated to be 10-25% or less during normal years but may extend up to 100 % during epidemic. Broadly, there are two breeding approaches adopted for disease resistance which includes conventional and molecular one. Conventional approach includes pureline, pedigree, backcross, recurrent selection and interspecific hybrids through which various resistant varieties has been developed. However, after sometime there is sudden outbreak of diseases due to continuous sowing of these varieties which results in the advancement in molecular biology and with the discovery of trait linked markers it paved a new pathway to look a new corner to develop varieties which are disease resistant. Molecular approach consists of gene pyramiding through which two or more genes providing resistance to different types of diseases is introgressed in a particular variety, gene deployment and multiline varieties which consists of heterogeneous population formed by mixing the cultivars to differ in the ability of disease resistance and reduces the rate of epidemic development. With the help of makers in marker assisted breeding using foreground, recombinant and background selection several linked markers are identified which provides resistance against disease like in wheat stem rust, leaf rust etc.

Introduction With the change in global scenario there is increase in pressure of meeting the food requirements of rapid growing population but due to scarcity of resources it is becoming very difficult to meet the needs of present population but even if there is so much problem still nature has provided solution to sort out from it through hybrid seed production through male sterility. As male sterility is a condition in which pollen grain is either absent or non-functional in the pollen grain. Its first case was reported by Koelreuter in 1763. Later many scientist have reported about its role in hybrid seed production. Mainly the male sterility is characterized by malformation of androecium, lack of normal anther sac, inability of pollen to mature or to release from the anther sac and inability to develop normal microspore or pollen. Due to such malformation male sterility is characterized into phenotypic male sterility, genotypic male sterility and chemically induced male sterility. Later in 1998, Kaul further generalised the phenotypic male sterility into structural, sporogenous and functional male sterility. In case of structural male sterility stamens are completely absent or if present then it is either malformed or modified into other floral part. While in case of sporogenous male sterility a case of abnormal microsporogenesis has been reported. In functional male sterility pollen development is normal but its function is impaired.Genotypic male sterility which is further classified into genetic, cytoplasmic and cytoplasmic genetic male sterility.

Introduction Agriculture is one of the promising sectors which offers marvelous potential for sustainable growth and play a key role in the economy of the country. Many of the foods that are already common in our diet are obtained from plant varieties that were developed using conventional genetic techniques of breeding and selection. Increase in the world population and limited food supply strained researchers to introduce and advance agriculture management and modern technologies along with conventional practices to attain highest possible crop productivity. Agricultural technology is based on the domestication of wild plants to create the crops that we have come to depend upon. Humans invented agriculture approximately 10,000 years ago when they began to harvest and cultivate specific plants to produce food. The improved plant traits selected by early agriculturalists were transmitted genetically to succeeding generations of plants. Both public institutions and private companies are now involved in crop genetic improvement.

Abstract Abiotic stresses have been a serious challenge for sustainable agriculture particularly vegetable cultivation characterized by decreased endurance against stresses like drought, temperature fluctuation and salinity. In India, drought and salinity being major abiotic stresses affect an area of 16% and 4.28%, respectively. Plant Breeders have to face many impediments while developing stress tolerant varieties. Developing tolerant varieties is quite difficult as tolerance varies with the developmental stages and has complex inheritance. This can be understood well by studying ontogeny of plants. Drought varies in time of occurrence, severity and duration. Field screening for tolerance is very difficult because of various interactions like drought × high temperature, high temperature × salinity interactions etc. Artificial screening in different stresses could be an alternative approach. The varieties developed with higher inputs at research stations fail to perform at farmers’ fields due to high genotype × environment interactions. Cultivated species have less genetic variability for abiotic stress tolerance but tolerance can be enhanced by using diverse and heterogeneous population. Though wild species possess genes for tolerance but these genes are closely linked with the undesirable gene(s) e.g. in tomato where S. cheesmanii, S. peruvianum, S. pimpinellifolium and S. sitiens have undesirable traits which affect yield and quality adversely.

Abstract Brassica vegetables belonging to family Brassicaceae are one of the most important groups of cool season vegetables. Initially, these crops were confined to Europe and other temperate regions. But, after the Second World War, their cultivation has spread to tropics and sub tropics as well. All the members of this group have originated primarily from the common ancestor wild cabbage (Brassica oleracea var. oleracea) and are fully cross-compatible with each other. In general, these are highly cross pollinated crops and have shown preponderance of non-additive gene action for most of the economic traits thereby suggesting the relevance of heterosis breeding. In the absence of appropriate genetic mechanism for the production of hybrid seed with ease at relatively lower cost, heterosis in a crop is of no practical value. Fortunately, the presence of natural genetic phenomenon of sporophytic self incompatibility and male sterility (particularly CMS) in brassica vegetables has proved instrumental in commercialization of hybrid seed production.

Introduction India is a rich center of plant diversity due to varied climatic conditions as well as soil qualities which provide a better platform for the diverse plant groups. In India around 45000 plant species are spread over the area (Mayers, 1988). These plant species multiply and produces the new plant. The multiplication of plants is termed as propagation and the art of propagation is as old as civilization of human being. The history of civilization began with the propagation and growing of plants. Plant propagation was in practice in pre-historic period. However, the propagation process has got commercial popularity only recently. The success in plant propagation depends on a thorough knowledge of the growth of plants, choice of suitable method and technical skill. In nature, plants multiply naturally to perpetuate themselves. Now-a-days, propagation practices have been totally modernized and became advanced from field to in vitro. Variation in plant propagation can be observed in the nature like some plants are producing many seeds for future generation with their unique type of dispersal mechanism. Among a large

Abstract A perennial plant or simply perennial is a plant that lives for more than two years. The term is often used to differentiate a plant from shorter-lived annuals and biennials. The term is also widely used to distinguish plants with little or no woody growth from trees and shrubs, which are also technically perennials. in vitro technologies are continuously expanding in the field of biology. Plant tissue culture has become a general title for a very broad subject. Commercial use of micropropagation is still limited, because of its relatively high production cost resulting mainly from high labor costs, low growth rate in vitro, and poor survival rate of the plantlets during acclimatization. Micropropagation of woody perennials is essential due their importance in production of secondary metabolites, which are playing a vital role now a days. Stages of micropropagation i.e mother plant selection, establishment of the aseptic culture, multiplication, root regeneration and transfer to the natural environment, which are systematically reproduced in many protocols which will aids in micropropagation studies of many woody perennials. Thus the review of present study attempts to provide a comprehensive account on micropropagation of woody perennials complementing conventional programmes for improvement.

Abstract Plant research often involves growing new plants in a controlled environment. These may be plants that we have genetically altered in some way or may be plants of which we need many copies all exactly alike. These things can be accomplished through tissue culture of small tissue pieces from the plant of interest. The latest list of ISO recognizes 109 spices, but only a few are important commercially such as black pepper, cardamom, ginger, turmeric, large cardamom, cumin, coriander, fennel, fenugreek, garlic, saffron and celery. They are extensively used in flavouring food and confectionery, perfumery and soaps and also as colouring materials. Many of these groups of crops have medicinal properties and are used in many Ayurvedic and Unani medicines. Inspite of the rich diversity and importance, the productivity of spices is low in India. Spices are an important group of economic plants with large genetic diversity in the Indian subcontinent and need to be conserved through in- vitro methods. Conservation of the germplasm using traditional method of field maintenance is problematic, normally labour intensive and also face risk of germplasm loss due to pest/pathogen attack or natural disasters. To minimize the problems of their conservation and increasing interest has been shown to use plant tissue culture. In vitro conservation of germplasm has been reported in various crop species including the spices black pepper, cardamom, ginger, turmeric, large cardamom.

Introduction Healthy seeds and seedlings are the first and essential requirement for achieving full yield potential of any vegetable crop. In recent years, vegetable growers have become highly conscious of the importance of quality seeds or seedlings. A major portion of the area under vegetable cultivation in India is now sown with hybrid seeds, which are costly but give higher yields and quality produce. In view of the high cost of seeds, some vegetable crops like tomato, brinjal, capsicum and cucurbits are being transplanted after growing nursery under protected conditions to achieve maximum germination count and healthy plant establishment. Raising of seedlings in plug trays or portrays is one such technology that achieves the above requirement. This technology is fast emerging as agro enterprise in India for its obvious advantage to both the nursery grower and the farmer. Soil-less substrate consisting of coco-peat and vermiculite is filled in plug trays and one seed is manually placed in each cell of the plug tray. However, manual seeding is labour-intensive operation requiring about more labour and time consuming. Mechanized precision seeding of plug trays is, therefore, necessary to enhance the capacity of rapidly growing nursery industry in India.

Introduction Controlled perpetuation of plants is known as plant propagation. Plant propagation can be broadly classified in two types: sexual and asexual. In sexual propagation, the fusion of male and female gametes gives rise to a new plant, whereas in asexual propagation which is also known as vegetative propagation, the development of new plants takes place without the fusion of male and female gametes, that is, the new plant develops from vegetative plant parts. Various techniques of asexual propagation are adopted in plant propagation, such as: grafting, budding, layering, cutting, etc. So far cutting and layering are concerned; the new plants are developed on their own root system, but in grafting and budding on the others’ root system. It means that in these two techniques (grafting and budding), two plant parts are joined to form new plants; the one which forms the root system is known as rootstock and the one forming the shoot system is known as scion. Plant propagation is both a science and an art. The science of plant propagation requires knowledge of plant physiology, nursery cultural practices, and characteristics of the particular plant that you want to grow. The art of plant propagation cannot be taught in a book or classroom, however, because it consists of specific technical skills that must be acquired through innate ability or experience and often requires a certain feel.