Preface Food Technology is an advanced and vast area of science and technology. It is an interdisciplinary field which encompasses different subjects like food microbiology, food chemistry, food engineering, human nutrition and so on. Though the art of processing and preserving food is well known since ages but the science behind these technologies is still not very clear. The basics of few of these technologies are well established; however, there is a need to overlook these technologies and the newer processing techniques which are developed in the recent past. Despite the fact that enormous literature prevailing to food science and its basic technique is available world around, the comprehensive book containing knowledge about innovative and emerging technologies of food is rarely found and if available it is scattered in individual units and hard to locate. So, it is the high need of time to compile a book possessing all these recent technologies and their know-how. Hence, we consider, it is of utmost importance to write the detailed and extensive book offering acquaintance to recent technologies emerged in food processing. We believe, it is a privilege to sum up the advances happening around the world in relation to food technology and its upgradation. The book is likely to cover the innovative technologies such as non-thermal technology, nanotechnology, non-invasive analysis of foods, newer methods of extraction, the recent know-how of food packaging, etc. This book will be very useful to everyone working in the area of food to upgrade their knowledge regarding various aspects of the latest processing technologies. The compilation, in particular, is not absolutely based on any specific lecture course. However, it will definitely serve as one of the affluent manuscript in supporting too many course outlines related to advanced food technologies prevailing in many academic institutions. This book will generate the interest of many courses including Emerging Technologies in Food Processing, Novel Food Processing Technologies, Advances in Food Technology, etc. Hence it will fulfill the high demand for food scientists and technologists in upcoming years and will gain popularity throughout the world. This will be an asset to all the readers thriving to upgrade their knowledge and utilize it for the betterment of mankind. The readers will get acquainted with latest happenings and its details in all aspects of food, thereby will add new dimensions to the basic research strategies. Academicians, researchers and students will get ready references to enhance their proficiency for emerging techniques in processing of foods since it is the compilation of novel technologies with all the details required.

1.1 Introduction and Background In present scenario, the word “extrusion” refers to a process by which dry or semi-moist ingredients with varying in-barrel moisture are forced through varying barrel temperature, screw speed and screw configuration through a die opening of the desired cross section. In other words, extrusion is predominantly a thermo-mechanical processing operation that combines several unit operations, including mixing, coating, kneading, venting, shearing, heating, forming, partial drying or puffing, depending on the material and equipment used. The origins of extrusion are in the metallurgical industry, where in 1797 Joseph Bramah patented the first extrusion process, wherein a piston driven device was used for making seamless lead pipes. The present understanding of extrusion technology and the developments or improvements in machine design are largely due to research carried out by the plastics industry. In food industry, this technology is in use since 1930 and its popularity is increasing continuously due to its efficiency, continuity and sustainability. In beginning, sausage extruders were developed in the nineteenth century as simple forming machines. One of the original developments which laid the foundation of the food extrusion was the use of pasta press termed as “cold extruder” in mid 1930s. Furthermore, in the late 1930s, General Mills, Incorporation., Minneapolis, MN, USA, first used a single screw extruder in the manufacture of ready-to-eat (RTE) cereals. Later in 1970s, twin screw extruders were used for the manufacture of moist pet-foods. Twin screw extruders were also adapted from polymer industry, as rising food quality and limitations of single screw extruders were demanding a more versatile process for the newer and higher quality products. After 1970s, the extrusion processing with simultaneous use of heat was popularized as a versatile and highly energy efficient with negligible wastage process. In all these years, number of changes has been noted in the extruder design such as changes in screw design, barrel design and also material used for the manufacture of these materials. Barrel liners availability along with already available segmented screws could further add more economics to the process and increased equipment lifetime. For better wearing characteristics, a number of screw elements of different materials are being used in addition to standard nitrided steel for customized processing needs, such as corrosion and wear resistant surface coated steels, stainless steel, satellite welded and bimetallic HIP (Hot Isostatic Press). JBL Feedscrews Limited, a UK based company recently introduced tungsten carbide coated screw in conjunction with a compatible bimetallic barrel. Likewise, a number of options for barrel metallurgy are available to withstand the higher internal pressure and temperature. Barrels lined with hard cobalt based alloy, typically Xaloy, to give abrasion and chemical resistance are also in use, although, the most common material is carbon steel with a nitride interior. Similarly, steam heated or jacketed barrels were used earlier for cooking purpose which gradually replaced by the electric induction heating elements. These HTST cooking extruders are versatile, highly productive and energy efficient processing machines. They can use a wide variety of raw materials and formulations to produce products with increased shelf-life and convenience.

2.1 Introduction Food is one of the complex systems involving many constituents such as carbohydrates, lipids, proteins, vitamins, minerals and other phytochemicals. These all constituents are not present in the same relative proportion in every type of food and thus the foods are categorized and valued according to the presence of these constituents as protein rich foods, fat rich foods, etc. The specific type of food is in abundance with only one or at most two of these constituents and rest of the bulk is not valuable, commercially. So, if these active constituents which are present in the tissue surrounding inactive matrix, are separated, it is of great benefit with respect to increased purity, nutritive value, functional properties, reduction in volume, easy handling and transportation. On contrary some valuable food items contain undesirable constituents generally referred as anti-nutritional factors. Removal of these anti-nutritional factors enhances the food value and commercial utilization. Hence, the separation of phytochemicals and removal of anti-nutrients from the food matrices can be easily carried out by using a unit operation called as extraction. Extraction involves the separation or isolation of active/desirable components of complex system viz., plant and animal tissues from the rest of inactive/undesirable components by using suitable means such as solvent or mechanical structure. The basic principle involved in the extraction process is partitioning of different components in accordance with the extracting agent. For example, in mechanical extraction, the partitioning is due to pressure and screen/apperature size where as in case of solvent extraction, the portioning of components from mixture takes place due to particular solubilizing power of solvent used.

3.1 Introduction Energy comes in many forms like chemical, radiant, mechanical, electrical, nuclear etc and can transform from one type to another. Energy can also be transferred or moved from one object to another. Electromagnetic (EM) radiation is a form of energy that can take many forms, such as radio waves, microwaves, X-rays and gamma rays even sunlight is also a form of EM energy. Visible light is a small portion of the EM spectrum, which contains a broad range of electromagnetic wavelengths. Electromagnetic waves, a self –propagating transverse radiation, are created when an atomic particle, such as an electron, is accelerated by an electric field, causing it to move. The movement produces oscillating electric and magnetic fields. The electric and the magnetic components of the wave are perpendicular to each other and also perpendicular to the direction in which wave is travelling in a bundle of light energy called a photon. Photons travel in harmonic waves at the fastest speed possible in the universe: 186,282 miles per second (299,792,458 meters per second) in a vacuum, also known as the speed of light. There are three important characteristics of the electromagnetic radiation, its frequency, amplitude and speed (Figure 3.1).

4.1 Introduction The purpose of processing the food is to make it safe and healthy for human consumption. The term food processing refers technologically anything, which transforms raw ingredients into consumer ready products, with the aim of stabilizing food products by preventing or reducing deteriorative changes in quality. The objective of food manufacturers is to develop, modify and employ those processing technologies that retain or create desirable sensory qualities in food and eliminate or reduce undesirable changes due to processing. The purpose of food processing is to increase the shelf life of food before spoiling (preservation); to remove, destroy or inhibit pathogens and toxins (food safety); to change flavor, texture, aroma, color or form (variety); to reduce preparation times and make food more portable (convenience); to restore and/or raise nutrient levels in food (nutrition).The various food processing methods used extensively in food industry include physical (high temperature, freezing, chilling, dehydration, packaging), chemical (control of storage gases, reduction of water activity, pH, addition of chemical additives or spices) preservation methods. Traditionally, food preservation has been associated with thermal treatments alone or in combination with biochemical or chemical methods. For example, the thermal processing of milk is done various thermal treatments viz. at 63oC for 30 min for low temperature long time (LTLT), 72oC for 15 seconds for high temperature short time (HTST) pasteurisations and up to 140oC for a few seconds for ultra high temperature (UHT) treatment. However, such thermal treatments effectively reduce level of microorganisms but in long-term preservation, it may lead to losses of desired organoleptic attributes and destruction of temperature liable nutrients and vitamins. The limitations involved with thermal processing has been overcome by employing chemical preservation, cool chain management and process hygiene in the formulation and production of food products.

5.1 Introduction Food is the basic requirement of every living being and it provides physical, mental as well as emotional satisfaction. Globally, there are many food enterprises which provide this basic necessity across the nations irrespective of nature of origin and climatic condition. Food companies are required to provide products at certain quality parameters so as to maintain the uniformity in the food system. Hence, food quality can be defined as a total of traits and criteria which characterize food as regard to its nutritional value, sensory value, convenience as well as the safety for a consumer’s health. Food quality is the quality characteristics of food that is acceptable to consumers. This includes external factors as appearance (size, shape, colour, gloss and consistency), texture, and flavor; factors such as grade standards and internal (chemical, physical, microbial). Due to the various food risks, all over the world the quality regulation authorities have intensified the efforts to improve food quality. Some of the quality related systems are obligatory by law while other is voluntary which are to be implemented by the various food chain members. Many companies are forced to improve safety and quality of their products by implementing the quality and safety assurance and management system because of growing consumers’ expectations and concerns as regards food quality and safety. In order to attain maximum profit and decrease the flaws, a new system was developed and followed by various companies all over the world. This new system is known as Six Sigma. The application of this system has the capability to reduce the losses considerably by reviewing the process parameters and increasing the profit exponentially. It constitutes a systematic approach to assure that food products have particular traits at any stage of production and distribution.

6.1 Introduction Consumer demand, regulatory guidelines and economic power of the consumer as well as ascertained profitability stimulated the need of novel food processing techniques. The concept of “fresh”, “natural”, or “organic” food also led to the development of environmentally sound and resource efficient food processing technologies. Thermal processes have been used for food preservation since a long time. These are not very energy efficient and can change the phase of food from fresh to cooked as well as it can alter the nutritional and sensory qualities. Thus, the quest for energy conservation by the manufacturers to reduce carbon footprint of the processes involved in food processing and preservation as well as to fulfill consumers’ requirement for fresh-like quality food have given rise to the development of innovative non-thermal food processing technologies i.e. ionizing radiation, high-intensity light pulses, high isobaric pressure, electric or oscillating magnetic fields, as well as pulsed electric fields (PEF). Application of electric energy in food processing started in the early 1900s. In the beginning, electrical pasteurization inactivated microorganisms by increasing the temperature of samples by means of an electrical resistance (ohmic heating). Milk was the first electrically pasteurized product. In the late 1940s, electric fields were used to increase the permeability of fruits to facilitate the subsequent extraction of juices. The PEF technology as a non-thermal process is very effective for inactivation of microorganisms, increasing the pressing efficiency, enhancing the juice extraction, and for intensification of the food dehydration and drying process. The main effect of PEF technology is based on the phenomena that biological membranes are punctured under the influence of external electrical impulses. This process is often referred as non-thermal, since the structural damage to membranes is implemented at very low temperature compared to the conventional heating process. The PEF process is considered to be energy efficient because the electric energy is released in short pulses, thus total energy requirement is much lower than other continuous preservation technologies. The microbial inactivation can be achieved at ambient or moderately elevated temperatures by the application of short-bursts of high intensity electric fields to liquid foods flowing between two electrodes or to semi-solid/ solid food between some specialized electrode and treatment systems. Due to chemical/physical properties and presence of electrical charge, the liquid foods (e.g. milk or fruit juices) had different conductivity, which results in variable flux of electrical current through the food systems.

7.1 Introduction Food processing has become one of the most competitive sectors in recent years. With increasing consumers’ demands, food industry needs to keep updating technology. These efforts are focussed at improving the organoleptic quality and nutrition of food and improve its keeping quality. This drives the industry to look for newer technologies which include new products as well as new processes. Some of the new processing technologies include non-thermal processes such as high pressure processing, pulsed electric fields and ultrasound processing. Advanced thermal processing techniques such as ohmic heating, inductive heating, extrusion and infrared heating have also been developed. Innovative methods for improving food quality through irradiation, removal of heat and moisture from food are now available. Newer packaging techniques now help to improve shelf-life and attractiveness of the food products and cater to the biodegradability of the packaging material. Hence, there is abundance of new products that have now become part of our daily life. Food nanotechnology has its long history when the first step of revolution in food processing and improvement in quality of foods was introduced by Pasteur to kill the spoilage bacteria (1000 nm). Later, Watson and Crick’s model of DNA structure, which is about 2.5nm, opened the gateway of applications in biotechnology. Nanotechnology is defined as the design, production, and application of structures, devices, and systems through control of the size and shape of the material at the nanometer (10-9 of a meter) scale. Nanotechnology is one such technology that has the potential to revolutionize the food industry. Taniguchi first used nanotechnology in 1974 to describe production technology at ultrafine dimensions. The word “nano” means “dwarf” in Greek and one nanometer is equivalent to 10-9 m. Objects of this scale are not visible through the human eye or the ordinary optical microscope. A nanometer (nm) sized particle measures one billionth of a meter. A human hair measures 80,000 nm while a DNA strand is 2.5 nm wide whereas a protein chain is 5nm in diameter. Nanotechnology can be applied to develop nanoscale materials, controlled delivery systems, contaminant detection and to create nanodevices for molecular and cellular biology. Nanotechnology may be defined as the understanding and management of matter at dimensions of roughly 1-100 nanometers. Unique behavior of matter at the nanoscale enables novel applications. Nanoscale science, engineering, and technology can be used for imaging, measuring, modeling, and manipulating matter at this length scale. Range of sizes of nanomaterials in the food sector and their relative position on nanoscale/microscopic scale are listed below.

8.1 Introduction Electron Microscopes were developed due to the limitations of Light Microscopes which are limited by the physics of light to 500x or 1000x magnification and a resolution of 0.2 micrometers. In the early 1930’s this theoretical limit had been reached and there was a scientific desire to see the fine details of the interior structures of organic cells (nucleus, mitochondria etc.). This required 10,000x plus magnification which was just not possible using Light Microscopes. The Transmission Electron Microscope (TEM) was the first type of Electron Microscope to be developed and is patterned exactly on the Light Transmission Microscope except that a focused beam of electrons is used instead of light to see through the specimen. It was developed by Max Knoll and Ernst Ruska in Germany in 1931. The first Scanning Electron Microscope (SEM) debuted in 1942 with the first commercial instruments around 1965. Its late development was due to the electronics involved in scanning the beam of electrons across the sample. Electron microscope has been a valuable tool in the development of scientific theory and it contributed greatly to biology, medicine and material sciences. This wide spread use of electron microscopes is based on the fact that they permit the observation and characterization of materials on a nanometer (nm) to micrometer (µm) scale. Electron Microscopes are scientific instruments that use a beam of highly energetic electrons to examine objects on a very fine scale. This examination can yield information about the topography (surface features of an object), morphology (shape and size of the particles making up the object), composition (the elements and compounds that the object is composed of and the relative amounts of them) and crystallographic information (how the atoms are arranged in the object). A scanning electron microscope (SEM) is a type of electron microscope that produces images of a sample by scanning it with a focused beam of electrons. The electrons interact with atoms in the sample, producing various signals that can be detected and that contain information about the sample surface topography and composition. SEM can achieve resolution better than 1 nanometer. The combination of higher magnification, larger depth of focus, greater resolution, and ease of sample observation makes the SEM one of the most heavily used instruments in research areas today.

9.1 Introduction Food quality and safety are central issues in food economics today. A number of developed countries (EU, UK, US) has imposed a number of new regulations to track food products across the supply chain which is forcing the companies to invest in new automated approaches for their implementations. These solutions facilitate better process efficiency with more productivity, quality control in production lines, lower manufacturing costs with higher profit margins, and also help to achieve improved presentation as customers request. The high labour costs in developed countries make cost-cutting inevitable to remain competitive production. The growth in products packaged for the market, the increasingly strict hygiene regulations, the need to reduce risks at work, cut costs, and control product quality are all calling for the development of technologies that enable robots to be used for these tasks. In fact, robotics has a great opportunity in this industry. But the variation in physical properties of various food products and the necessity to avoid damaging them, make it necessary to seek flexible handling solutions for a wide range of products. The robotics industry is looking into a bright future. As per the report given by the Statistical Department of International Federation of Robotics (IFR), it was projected that between 2014 and 2016 worldwide robot sales will increase by about 6% on average per year. Ten years ago, global sales figures were hovering around 80,000. So, three key factors for the boom - factory modernization, increases in production capacity and rising demand from a number of emerging markets.

10.1 Introduction Food safety and quality are the two important factor which results in consumer food choices. Nowadays consumers are demanding food which is safe and of high quality in terms of nutrition due to a healthier life style. Food safety and quality are the terms used interchangeably. Food safety refers to all those hazards, that make food injurious to the health of the consumer and it is not negotiable. Food quality includes attributes that influences the product's value to the consumer. This includes both negative and positive attributes; negative attributes includes spoilage, contamination, discoloration, off-odours and positive attributes include colour, flavour, texture and processing method of the food. This distinction between safety and quality has implications for public policy and influences the nature of the food control system. Food control is defined as a mandatory regulatory activity which provides consumer protection and ensures food is safe, wholesome and fit for human consumption during production, handling, storage, processing, and distribution. Despite the huge efforts made by the food safety authorities and industries, food safety still remains a critical issue. In food industries, producers have to satisfy both safety and quality criteria for their products to the consumer. Food processors have multiple options in terms of different quality and management systems, they have to choose the most appropriate one for its specific activity and should establish, document and implement effective systems in the processing area for managing quality and safety of the product. Over the past few decades, food-borne illnesses have raised great concern about the safety of food. Considerable steps are taken by the food processor for understanding and managing the risks of airborne diseases. Among various systems, Hazard Analysis and Critical Control Point (HACCP) is effective tool which focuses mainly on controlling hazards to ensure the production of safe and wholesome food. For maintaining the safety and increasing the shelf life of food, traditionally it has been preserved by thermal processing, freezing, salting and drying. But nowadays consumers are demanding convenient, innovative, fresh foods, including new "minimally processed" products. To meet consumers' expectations in the 21st century, the food industries are implementing novel technologies whose purpose will be two fold: 1) to provide the new quality attributes demanded by consumers; and 2) to ensure the all important and often expected assurance of food safety. The novel technologies will include various minimally processed technologies like high pressure processing (HPP), pulsed electric field (PEF), ohmic heating, dielectric heating, microwave heating, ultrasound, modified atmosphere packaging (MAP) and cleanroom technology. Among these cleanroom technology have attracted great attention for maintaining the safety and quality of the food products as because it doesn't have any effect on the nutritional quality of the food being processed, it maintains the hygienic condition in the processing area, remove contaminants from the processing area and clean air in the processing area which in turn results in safe food product. The main objective of cleanroom technology is to eliminate airborne contaminants from the humans and/or from the environment. An air curtains and a positive air pressure is maintained in the processing unit using filtered air. Production personnel will use the special clothing which is preferred for cleanroom to reduce the contamination from both air- and human-borne microorganisms. In this chapter we will discuss about cleanroom technology, its historical background, its classification, layout of cleanroom, air handling, personnel and its clothing, regulations in cleanroom, and its application in food industries.

11.1 Introduction Phytosterols or plant sterols are bioactive plant constituents having similar chemical structure and biological functions as cholesterol (cholest-5-en-3b-ol), but differ in their side chain configuration. They are non-nutritive compounds with chemical structure differing from that of cholesterol only by minor modifications like in the structure of their side chain by a methyl or ethyl group at C-24. Sitosterol and campesterol have an ethyl and a methyl group at C-24, respectively (Fig. 11.1). Phytosterols present a tetracyclic ring and side chains linked to C-17 that differ according to sterol compound same as cholesterol, to which they resemble structurally as well as biosynthetically. Saturated form of sterols referred to as stands, occurring in small amounts, mainly in cereals are represented by the absence of the double bond at the D5 position. Phytosterols represent a part of the broad group of isoprenoides and are 28- and 29-carbon atom steroid alcohols naturally occurring in plants. Phytosterols and phytostanols are triterpene compounds, found ubiquitously in plants which can only be synthesized by plants, whereas humans and animals obtain them from their diet.

12.1 Introduction Micronization is a size reduction in which particles are reduced down to micrometer resulting in particle-size distribution is less than 10 microns. Acceleration of particles has been involved for particle-to-particle impact or impact against a solid surface. Micron technology is an emerging technology which has a great potential in nutraceuticals and functional foods. By increasing the surface area per unit mass, compared with larger-sized particles of the same chemistry, tiny particles are made more biologically active. A lot of attention has been gained by micronization in food research and development. Functional properties, surface areas and structures gets altered by reduction in particle sizes of different materials (e.g., metal salts, metal oxides, chitosan and organic compounds) by micronization or nanotechnology to micro- or nano-sizes and bring some new applications in the academic world as well as in food industry. They absorb a large concentration (10-40 wt %) of C02 that either swells the polymer or melts it at a temperature below (10 to 50 °C) it’s melting/ glass transition temperature hence, this technique is useful for micronization of especially for polymers. Active substrate(s) in a polymer or other carrier substance can be used with suspensions for micronization process. In manufacturing advanced pharmaceuticals, coating materials, micro sensors, polymers and many other chemicals particle design has been gaining importance. Formulation homogeneity and control particle size can be improved by micronization. Moreover, bioavailability of poorly soluble APIs (active pharmaceutical ingredient) by increasing particle surface area and accelerating dissolution rates can also be improved. Because of the high impact velocities possible as a result of particle acceleration in a fast gas stream, fluid energy mills are used for micronization. Particle velocities in a jet mill are in the range of 300 to 500 meters per second, compared to 50 to 150 meters per second in a mechanical impact mill, in many industrial fields like food technology, pharmaceuticals, catalysts, pigments, and biopolymers, for example, are some categories of products that can be used as micro-sized particles, micronized powders are of great interest. High-temperature reactions that require high energies, on jet milling that is characterized by low efficiencies and mechanical stress, and on liquid solvents precipitation that has a poor control on particle size and can pollute the product comes under the traditional techniques for producing micronic powders. Generally, the control of the powder size and the span of its distribution are still very approximate. In recent years, many supercritical fluids-based techniques have been developed for the production of micronic and nanometric particles. Some particular properties of gases at supercritical conditions such as enhanced solubilization modulation and its power, large diffusivities, organic solvent reduced operation, and the connected possibility of controlling powder size and distribution resulted from this process. Important characteristics of powders like particle size and size distribution have significant effects on dissolution properties, flowability and release kinetics. Therefore, particle engineering is the most important step during processing of powdered foods. Lower micrometer range and sizes are needed for many applications particles with special requirements. Processes like jet milling, pearl milling or high pressure homogenization are used for micronization.

13.1 Introduction A surfactant or surface-active agents are the compounds that lower the surface tension between the two liquids or between a liquid and a solid. Surfactants may act as detergents, wetting agents, emulsifiers, foaming agents and dispersants. Surfactants are normally organic compounds that are amphiphilic, meaning they contain both hydrophobic groups or hydrophilic groups. Therefore a surfactant contains both a water insoluble component and a water soluble component. Surfactant will diffuse in water and adsorb at interfaces. The interfacial free energy is the minimum amount of work required to create that interface and the interfacial tension between two phases is determined as the interfacial free energy per unit area. The interfacial (or surface) tension is also a measure of the variance in nature of the two phases meeting at the interface (or surface). The greater the dissimilarity in their natures, the greater the interfacial (or surface) tension between them. A surfactant is therefore a substance that changes the amount of work required to expand those interfaces. Surfactants usually act to reduce interfacial free energy. Surface active agents in the form of emulsifiers have a long history of use and are widely used in many products being used in everyday life. An extensive basic research is required to understand the reason of instability and methods to prevent the breakdown of emulsions. Emulsifiers can function as dough conditioners by improving tolerance to variations in flour quality, better gas retention resulting in lesser yeast requirements, increased uniformity in cell size, a finer grain, more resilient texture and improved slicing. Also, the characteristics of freshly baked bread like soft crumb can be retained longer, if the appropriate emulsifiers are added. Commonly used surface active agents in bread production are propylene glycol mono- and diesters, polyoxyethylene sorbitan monostearate, ethoxylated mono- and diglycerides, succinylated monoglycerides and Diacetyl tartaric acid, esters of monoglycerides. Cookie characteristics like top grain, volume and spread ratio can also be improved. Here surfactants used are sodium stearoyl fumarate, sucrose fatty acid esters, sodium stearoyl lactylate, sorbitan fatty acid esters, polysorbates and ethoxylated monoglycerides. In dairy products like Ice cream surface active agents play some important functions like to improve fat dispersion and control fat agglomeration, facilitation of fat– protein interactions, facilitate air incorporation, give smoother texture due to smaller ice crystals and air cells, increase resistance to shrinkage, improve melt-down and reduce whipping time. In candy products, the elimination of “bloom,’’ i.e., the transition of fat crystals is a key reason for the addition of surface active agents. In mixtures of triglycerides, surfactants like sorbitan esters (Spans) and ethoxylated sorbitan esters (Tweens) can control product viscosity in chocolates and in cream fillings and modify the crystal structure. Surface active agents have been utilized in the production of meat analog products also. In margarine, Emulsifiers fulfil several functions like modification of crystal structure in the vegetable fat, assistance in emulsion formation and antispattering. Here, a mixture of citric acid monoglycerides and monoglycerides or lecithin can be used. The properties and mode of action of these chemical surfactants is discussed in the following sections.

14.1 Introduction Proteins are the substances formed from nitrogen containing amino acids and are principle structural components of body tissues and muscle. In addition, they are used to produce various hormones, enzymes and hemoglobin in human body. Due to the shift from agricultural to electronics and other fast paying industrial development, most of the countries around the world, ceased to be self-sufficient in their food production in particular protein, which is necessary to satisfy their optimum food demands. The biotechnology development and its usage to agriculture have brought hope to them. The need for food by modernization of agriculture has gained much attention throughout the world. However, the necessity for exploring unconventional and non-agricultural means of food production, especially of proteins rich food, cannot be over-emphasized due to the tremendous growth of human populations in several regions of the world. In addition to this, the importance of protein in food nutrient cannot be put aside because its shortage causes various malnutrition problems and the most lethal malnutrition problem is Protein-Energy-Malnutrition (PEM). The United Nations Food and Agriculture Organization estimates that nearly 870 million people of the 7.1 billion people in the world, or one in eight, were suffering from chronic undernourishment in 2010-2012. There are 925 million people undernourished in developed countries (FAO October, 2010). This situation has created a demand for the formulation of innovative and alternative proteinaceous food sources having high nutritional value. These sources have to be non-competitive with food for human consumption, economically feasible and locally available. This demands a search for new protein sources, with high nutritional value, economically feasible and locally available. Use of microbes as a food source is one of the biotechnological innovations that will certainly increase the availability of affordable protein in the world to solve the global food and feed problems. In recent decades there has been an increased interest in the industrial production of microbiological protein for use in both human and animal nutrition. The main reason behind it is that there has been the shortage of low cost protein sources in view of the increasing human population and living standards, resulting in famine in some parts of the world and increased demand for animal protein in others.

15.1 Introduction Color of food is the most sensitive part of any commodity as it is the one of the organoleptic characteristic to be noticed and one of the main ways of visually assessing a food before it is consumed. The people associate certain colors with certain flavors and the color of food can influence the perceived flavor in anything from confectionary to beverages. For this reason, food manufacturers add dyes to their products. Sometimes the aim is to simulate a color that is perceived by the consumer as natural, such as adding red coloring to glace cherries, but sometimes it is for effect, like the green ketchup that was launched by Heinz in 2000. If the flavor of a food product is inconsistent with the color, the flavor can often be perceived incorrectly, for example an orange flavored drink colored green could be perceived to taste of lime. In addition, the color of a food substance is important indicator of its freshness. While all of us are aware that food with bright or unnatural colors likely contains food coloring but only few people know that natural foods such as oranges and salmon are sometimes also dyed to mask natural variations in color. The color variation in foods throughout the seasons and the effects of processing and storage often make color addition commercially advantageous to maintain the color expected or preferred by the consumer. Some of the primary reasons include:

16.1 Introduction Food safety is most important issue in today's global environment. To safeguard food supply is a very difficult and challenging responsibility, as a consequence of which the world's largest food companies, retailers, and manufacturers have placed very high food safety standards. Although the safety of food has improved exponentially but progress is uneven and food borne outbreaks due to microbial contamination, pesticides, antibiotics chemicals and toxins are common in many countries. International trade statistics (2007) by World Trade Organization (WTO) reported that Europe has accounted for 46% of world exports of agricultural products, where food represents 80% of agricultural exports. The trading of contaminated food among countries increases the risk for outbreaks and consequently, health risks due to microbial pathogens in food are of major concern to all governments. Biosensors are an important option in the agricultural and food sectors to control production processes and ensure food quality and safety by dependable, fast and cost effective methods. Biosensors have plenty of advantages over conventional analytical tools since they offer advantages in size, cost, inherent specificity, selectivity, rapid response, accuracy and sensitivity. Food microbiologists are always in search of rapid and reliable automated systems for the detection of biological activity. Biosensors provide sensitive, miniaturized systems that can be used to detect unwanted microbial activity or the presence of a biologically active compound, such as glucose or a pesticide. Immunodiagnostics and enzyme biosensors are two of the leading technologies that have had the greatest impact on the food industry. The use of these two systems has reduced the time for detection of pathogens such as Salmonella to 24 hour and has provided detection of biological compounds such as cholesterol or chymotrypsin. The another useful application for biosensors, is detection of genetically modified organisms (GMO), since several countries have laws regulating the commercialization of GMO products and their derivatives. Biosensors are also useful in the implementation of hazard analysis and critical control points (HACCP) plans by verifying process developments and correcting errors in due time. There is joint application of Biosensor technologies and nanotechnologies in many food and agricultural applications such as the development of nano-scale tools for biosafety, nanoscale compounds for food packaging, and nano-sensors for pathogen detection in animals and plants. These are helpful tools in the detection and control of potential food contaminants by the agricultural and food industries.

Due to inadequate intakes of vitamins and minerals, half of the world's population suffers from micronutrient malnutrition. An adequate amount of wholesome as well as diverse foods are required for the proper growth and development. In addition to calories requirements, these foods should provide various nutrients. In India, ~70% of children do not get the required calories either due to lack of enough food or diverse food. This indicates that these children are also incapable of fulfilling their protein, minerals and vitamins requirements. Diets that lack diversity can be deficient in certain nutrients. Due to the micronutrient composition of the soil of a region, or because of the intrinsic insufficiency of the normal diet the staple foods of some region can lack particular nutrients. The individual plant foods are often deficient in certain nutrient components due to the varying level and composition of food nutrients in different food crops. Some crops are rich in one component but low in other. For example; legumes are usually high in protein, but deficient in essential amino acids like methionine whereas, wheat and rice are rich in carbohydrates but contain little essential amino acid such as lysine, iron, and lack provitamin A. Relying on a single food crop as major staple source of nutrients thus will not attain a nutritionally complete diet and result in malnutrition and deficiency diseases, which often occur in populations of developing countries, mainly due to poverty. Globally, over one billion people are undernourished and food security is a major issue with the world's population forecast reaching to nine billion by 2050.

18.1 Introduction The Packaging Institute International describes packaging as the enclosure of items, packages or products in a wrapped pouch, box, bag, cup, can, tray, tube, bottle or other container for protection, preservation, containment, communication, performance and utility. To an engineer or a scientist, it is technical matter as it is a system designed for efficient delivery of safe food products with high quality, via each and every stage of distribution chain, from production of raw material to food manufacture, packaging, retail, wholesale, consumer, disposal and finally recycling or of resource resurgence. The extent of food packaging is literally broad as it encompasses technological activities such as graphic and machinery design, package manufacture and development, shelf life testing, distribution and marketing. Food packaging science is a discipline which applies principles from four major areas of science (materials science, food science, information science and socioeconomics) to understand the properties of packaging materials, packaging requirements of foods and the packaging system. Both material science and food science have been two major contributors for food packaging development. Material science is important to understand the mechanical strength, barrier properties, appearance and physical and chemical properties of paper, glass, metal, polymer and composite. Food science is important to understand the deterioration kinetics of foods (such as microbial growth, lipid oxidation, moisture loss or gain) governs the food shelf life. Food packaging technology is a science based solutions to address specific food packaging needs; examples are tamper evident packaging, microwavable packaging, modified atmospheric packaging, and controlled atmospheric packaging which are aimed at enhancing safety, quality and convenience for the consumer.

19.1 Introduction Food safety is one of the important factor for food industry, regulatory agencies and consumers. Nowadays consumers are demanding food which is safe and of high quality in terms of nutrition due to a healthier life style. Food safety refers to all those hazards, that make food injurious to the health of the consumer and it is not negotiable. Food may be contaminated physically, chemically or microbiologically, mainly microbial contamination is of high concern in the food industries. Various pathogenic and spoilage causing microorganisms, viruses, insects can deteriorate the food products and cause food borne illnesses. Therefore, there is a need to eliminate these undesirable microorganisms from the food industries to ensure food safety to the consumer. Various methods are available to eliminate these undesirable microorganisms such as thermal technologies like pasteurization, dehydration, retort processing, freezing etc. and non thermal technologies like microwave heating, Pulsed Electric Field (PEF), High Pressure Processing (HPP), Pulsed Light Technology, Ohmic Heating, Ultra sonics, Pulsed X-Rays. But these technologies have certain disadvantages like thermal technologies lowers the nutritional value and leads to unwanted changes in the foods' sensory attributes of the food products whereas non thermal technologies retain better nutritional and sensory quality of food than thermal technologies but they are cost effective, required specialised equipments and trained personnel. Cold plasma technology (CPT) is a non-thermal green technology used now-a-days for variety of application in various industries especially in food industry. It is proposed to be an alternative method for eliminating microorganism from the fresh produce. This technology does not require extreme process conditions and can be used as a good alternative source for food preservation without affecting its nutritional and sensory attributes. Plasma is an ionized gas, consisting of active species like positively and negatively charged ions, electrons, free radicals, and gas atoms, metastable and quanta of electromagnetic radiation (photons). When the energy source is turned off, active species used to disappear immediately, therefore cold plasma technology is environmental friendly and fulfilling all ecological standards. Based on the properties of plasma, it can be applied in various fields like electronics, life sciences, textile, packaging and food industries. In food industries, it is showing promising application by retaining the nutritional, functional, and sensory characteristics thus ensuring the fresh appearance of the food product and satisfying the consumer need.