Preface To serve the nation in terms of nutritional food security, safety and employment generation is the prime duty of food scientists. To upgrade the national economy with the improvement in the quality, quantity and safety is need of today. Another challenge ahead is to meet out the daunting demand of modern consumers with high quality protein. Meat is one of the most significant and easily available food sources with high biological value protein. Meat industry is also capable to uplift the status of rural people and employment generation in rural areas. However, in India, little attention has been paid to the meat sector though it contributes about Rs 25984 crores every year in total livestock output. This sector is not only important for providing meat and by-products for human utility but also contributes for sustained livestock production and livelihood activities in India. The approach to obtain efficient livestock production, processing and utilization now becomes more stringent due to consciousness of consumers and competitive environment created through trade liberalization by World Trade Organization (WTO). So keeping all these facts in mind the 2nd edition of the book titled “Principles of Meat Technology” is upgraded with the vast material and recent innovations in meat science. Each and every aspect of the meat science right from production of meat to ultimate consumption is very well illustrated and documented. The recent aspects like organic meat production, genetically modified food of animal origin and various types of standards on meat, fish and poultry products are very well incorporated in the present edition. In other words all the requirements of the today’s students of veterinary science, food science, meat technology and other related fields are taken up from basics to the modern. Thus it is expected that the veterinary professionals, students, industry personnel and other professionals of allied fields may continue their support for acceptance of the present edition. The improvement and upgradation is a continuous process. So the critical comments and suggestions on various aspects of this book will help us in improving this manuscript in future.

In spite of big potential of the largest livestock population in the world, meat industry in India is still in nascent stage. It is a business of those who are not trained and very much literate. The industry is primarily based on the raw material that is not reared for meat purpose but serves the secondary role after passing their viable life in primary field. The industry is also facing problem of social prejudice and meat is taboo for certain communities. But scenario is now changing and people are coming forward to organise the sector. Now the society is accepting the facts that meat is an only source of cheap and nutritious food to million of people in India and the good source of animal protein for large segment of Indian population, particularly landless, small and marginal farmers. India ranks fifth in the world meat production with the annual meat production of about 6.3 million tones which is 2.21% percent of world meat production. In the last two decades meat industry in India has grown @ 4.5% whereas, during the last five years, this segment has been growing very fast at the rate of 27% annually. It has a good future if given proper attention by the Government and Private Entrepreneurs. In total meat production, share of cattle is 17.34%, buffalo 23.3%, sheep 4.6%, goat 9.4%, pigs 5.3%, poultry 36.6% and others 3.4%. The share of various meat types in total Indian meat production accounts buffalo meat 1.5 million tones, beef 1.28 million tones, mutton 0.24 million tones, chevon 0.53 million tones, pork 0.50 million tones, chicken meat 2.20 million tones and duck meat 0.07 million tones. To produce the above quantities, the extraction or slaughter rates in cattle are about 7.9%, buffaloes 11.1%, sheep 47.9%, goat 37.9%, pigs 88.9% and poultry 73.6%. In the world total meat production Indian cattle meat shares 13.1%, buffalo meat 55.8%, mutton 5.7% and chevon 14.9%. However, India is having world’s 15.0% (210 million) cattle, 57.0 %( 111 million) buffaloes, 7.0 %( 74 million) sheep, 17.0 %( 154 million) goat and 1.5% pigs. Overall contribution of meat sector in total output of Livestock sector was Rs 25984 crores in the year 2004-2005.

The word meat comes from the old english word mete, which referred to food in general. Most often this means the skeletal muscle and associated fat but it may also describe other edible tissues such as organs, livers, skin, brains, bone marrow, kidneys and lungs etc. In other words we can say meat is those animal tissues which are suitable for use as food by the man. It is composed of numerous types of tissues like muscle tissues, epithelial tissues and nervous tissues but the major component of meat is muscle. Particularly skeletal muscle is the principal source of muscle tissue in meat while smooth muscle contributes little to meat. The muscle and connective tissues are the major compositional components and contributes towards qualitative and quantitative characteristics of meat. Broadly muscle can be classified into striated and smooth muscles. Striated muscles have transverse band pattern as seen in skeletal and cardiac muscle. However, smooth muscles do not show such pattern and mainly found as a component of blood vessels.

Conversion of muscle to meat is a complex process in which several physical and chemical changes takes place over a period of several hours or even days. The major changes which take place during this process include fall in muscle pH and temperature as well as muscle shortening which further leads to proteolysis or ageing. These changes during conversion of muscles to meat are variable and have great effect on meat quality (tenderness, juiciness, flavour, colour etc.) and also on certain processing characteristics like emulsification, binding properties and yield of the products. To know this complex process it is necessary to understand the process of energy production and metabolic pathway shifting in live animals. Pre Slaughter In the living animal, aerobic metabolism is used to obtain energy. The source of energy at this stage is either free fatty acids or blood glucose or muscle glycogen. In this process total 38 ATP molecules are produced from single glucose or glycogen molecule by the complex processes known as glycolysis, oxidative decarboxylation and oxidative phosphorylation. However, under stressful situations, anaerobic metabolism may be used and lactic acid is produced. Lactic acid is usually transported from the muscles to the liver, where it is re-synthesized into glucose and glycogen, or to the heart, where it is metabolized to carbon dioxide and water.

Meat is an animal flesh that is used as food. Most often, this means the skeletal muscle and associated fat but it may also describe other edible tissues such as organs, livers, skin, brains, bone marrow, kidneys or lungs. Meat is highly nutritious food because it contains protein, fat and carbohydrates in relative proportion, supply energy as required by the human body, posses ability to build or replace tissues and the substances present in meat are easily absorbed by the human body. Meat is also a good source of B-complex vitamins but fat soluble vitamins are low in meat. It is a good source of phosphorous, zinc and iron but low in calcium. Meat also contains significant amount of copper, sodium, potassium and magnesium but low in carbohydrate contents. Carbohydrate is found mainly in form of glycogen which disappears completely after completion of rigor mortis. In general lean red meat is particularly good source of protein, niacin, vitamin B6, vitamin B12, phosphorus, zinc and iron. About 100g of meat can provide more than 25% RDI of these nutrients. It also provides more than 10% RDI of riboflavin, pantothenic acid and selenium. Of the four meats, mutton is particularly nutrient-dense and the richest source of thiamine.

Fraudulent substitution of meat is the adulteration or mixing of the inferior or cheaper quality meat into superior quality meat. The first case of fraudulent substitution was recorded in thirteenth century A.D. at Florence in Italy. A common fraud in the meat industry in which uninspected meat is substituted for meat that has undergone inspection and been branded as satisfactory. The other frauds are the substitution of meat of another species, e.g. horse for beef especially in Britain and Ireland, beef in kangaroo meat in Australia, cat for chicken or rabbit, goat meat for mutton, mutton for venison, dog meat and cat meat for chevon in other countries including India. As per an estimate about 25-30% of meat sold in India is adulterated. These practices are more common in comminuted meat products. These illegal practices are punishable under PFA act 1955. The choice of method to be applied is depend on the need or condition of meat recovered.

Preservation of foods including meat and aquatic foods is a creation of conditions for the food articles in such a way to stop or greatly slow down the activities of micro-organisms, chemical and enzymatic reactions and physical forces to prevent the meat and aquatic foods from spoilage while maintaining its nutritional value and sensory attributes. Significance of Meat Preservation Meat and aquatic foods are perishable food items and are very prone to spoilage and rancidity development due to high nutrients, moisture and neutral pH. Once meat and aquatic foods is exposed to microbial contamination, chemical and enzymatic reactions then it will be very difficult to attain asepsis. To enhance the keeping quality of meat and aquatic foods so that it will be available for further processing. It protects meat and aquatic foods from shrinkage, sweeting and other physical forces. To protect human health by providing the wholesome meat and aquatic foods to the consumers. By this way we can minimize utilization of energy in processing of meat and aquatic foods.

Ageing of meat is also known as conditioning or tenderising or ripening or maturing and sometime resolution of rigor. It is a process of holding unprocessed meat above freezing point under controlled conditions. By this process we can make muscle soft and pliable thus meat becomes tender. When we keep the meat above freezing point at temperature between 0-3ºC then all those changes occurs which are normally occurs at higher temperature i.e. atmospheric oxidation and enzymatic action of fat, bacterial action etc. When this temperature range becomes below freezing point then it inhibits completely the enzymatic action in the muscles. Ageing is required for marked increase in flavour, juiciness and tenderness of meat. In this process action of enzymes in meat plays key role while bacterial action is not of any importance. Meat tenderness and taste are definitely improved if carcasses or vacuum packed cuts are conditioned after slaughter but paradoxically beef is quite tender just two hours after slaughter and several days of conditioning are required to recover this degree of tenderness. During the contraction of muscles, thick and thin filaments sliding to each other and decreases the length of sarcomeres. In turn, this decreases the length of fibrils, fibres and the whole muscle. If these muscles remain in contracted length when rigor develops, it causes massive overlap of thick and thin filaments leading to severe meat toughness. It is also necessary to avoid the cooling of meat too rapidly after slaughter, otherwise it cold-shortens and gets tough. If we avoid cold-shortening of muscles during conditioning, it will increase meat tenderness. But with too much conditioning, weight loss, surface spoilage and the cost of refrigerated storage increases. Ageing of carcasses with little or no fat cover is not recommended by meat specialists. Because these carcasses loose moisture rapidly, loose weight excessively and discolour the meat surface rapidly. In addition, lean meat is also susceptible to deterioration through microbial growth, slime formation by bacteria and mould growth etc. Ageing is important for beef and buffalo meat while pork and lamb do not require ageing because they are slaughtered in the age where they are young and inherently tender. Additionally, the unsaturated fats found in pork fat oxidize during ageing causes rancidity and off flavour. Veal has very little protective fat covering and is high in moisture; thus it does not lend itself to ageing.

Meat processing is the set of methods and techniques used to bring substantial chemical and physical changes in the natural state of meat. These are the methods which are used for making meat and their products attractive, nutritious and marketable as well as for the prolongation of shelf-life. In processing techniques, grinding, chopping, addition of seasoning, alteration techniques for colour, heat treatments etc. are included but cutting, trimming and deboning are not included in processing operations. History Meat processing dates back to the prehistoric ages and probably the first processed meat product was sundried meat. Lator it turns to burning of meat over wood fire while salting and smoking of meat was practiced first time in the time of Homer, 850 B.C. In 63B.C.-14 A.D. at the reign of Augustus honey was tried for preservation of meat. Lator various types of cooking such as roasting, smoking, steaming and oven baking came in existance. Salt-preservation was especially common for foods including meat and meat products for warrior and sailor’s diets, until the introduction of canning methods. Modern food processing technology in the 19th and 20th century was largely developed to serve military needs. In 1809 Nicolus Appert invented a vacuum bottling technique that would supply food for French troops and this contributed to the development of tinning and then canning by Peter Durand in 1810. Although initially expensive and somewhat hazardous due to the lead used in cans. Canned goods would later become a staple around the world. Pasturization discovered by Louis Pasteur in 1862 was a significant advance in ensuring the micro-biological safety of food. In 20th century, food processing advances with the development of spray drying, freeze drying and the introduction of artificial sweeteners, colouring agents and preservatives such as sodium benzoate. In late 20th century ready to eat meat food products were introduced to cater the need of consumers.

Food packaging is an essential technique for preserving food quality and minimizing food wastage. It is a scientific technique of containing food against physical, chemical and microbiological contaminants. In addition package also display the product in most appropriate manner and provide product information to the consumers while at the same time allowing convenient handling and efficient distribution. The packaging of meat and meat products are particularly important because meat is highly nutritious food and provides good medium for the growth of micro-organisms. Meat and meat products are also susceptible to bacterial growth due to their high water activity. Exposure of meat and meat products on atmospheric temperature and sunlight leads to various types of deteriorative changes. For example when cut meat surfaces exposed to ambient air provide excellent breeding grounds for most bacteria. Minced meat is even more at risk due to the large exposed surface area. Packaging provides protection of food against external and internal factors but once food gets deteriorated it can not be controlled by packaging. For this reason rigorous hygiene in all processing and pre-packaging steps is vital for packaging of perishable food items like meat and meat products.

Formulation of meat and sea food products requires various ingredients which are commonly known as food additives. Food additives are those non-meat ingredients which are used to enhance colour, flavour, aroma and texture of the meat and sea food products. These food products can fulfil the demand of modern consumers. As per the definition of Food and Drug Administration (FDA) food additives are those substance which are used to provide a technical effect in foods. The use of food additives has become more prominent in recent years, due to the increased production of prepared, processed, and convenience foods. Additives are used for flavour and appeal, food preparation and processing, freshness, and safety. These additives can be classified as curing ingredients, extenders and binders, flavour enhancers and other additives. For the formulation of different products both raw materials and ingredients should be of food grade and sound hygienic strength. In the starting of this chapter we will discuss about ingredients used then the specific formulations will be discussed for better understanding of meat and sea food processing. Curing Ingredients Curing agents such as salt, phosphate, sugars, nitrite and nitrate, sodium ascorbate and erythorbate etc. have traditionally been used in various meat formulations from long back. The details of these ingredients have already been discussed in chapter-8 of this book. So we will try to discuss other ingredients which are relevant for meat and sea food products other than these ingredients. Cure accelerators such as ascorbates and erythorbates are used to speed the curing process. They also stabilize the colour of the final product. The examples are ascorbic acid, erythorbic acid, sodium erythorbate, citric acid, sodium citrate, sodium acid pyrophosphate, glucono delta lactone (GDL) etc. Glucono-delta-lactone (GDL) acts as a curing accelerator in meat and meat products. It is permissible up to 1% in sausages and up to this level it lowers the pH about 0.5 thus inhibits the growth of spoilage micro-organisms. GDL also facilitate the cured colour development.

Physico-chemical and microbiological quality of meat and aquatic food and food products gives its overall safety and quality status as a food. The physico-chemical and microbiological qualities of raw material have great importance on the product development. It also affects the shelf life of the raw material as well as final product. Fresh meat is a type of meat which has not been subjected to any processing whereas raw meat is a fresh meat which has undergone freezing. The qualities of fresh meat are important as far as merchandiser, purchaser and consumers are concerned and it is also important for adaptability in further processing. The important quality parameters of fresh meat are:

Sensory evaluation is a scientific method of evaluating foods including meat products that applies principles of experimental design and statistical analysis through the use of human senses like sight, smell, taste, touch and hearing. It is also called organoleptic evaluation in which we measure, analyse and interpret reactions to those characteristics of materials as they are perceived by the human senses. These attributes can be perceived well by the human sense organs as compared to instruments available for this purpose. It is a fact that no single instrument is able to assess all the attributes of meat products. With the help of these instruments we can very well evaluate the single quality attribute of meat product i.e. shear force instrument or penetrometer is good for knowing the texture and refractometer is for colour interpretation.

Eggs are considered as Nature’s original functional food because they are abundant source of nutrients like vitamins and minerals. It contains valuable nutrients from the high-quality protein to significant levels of beneficial vitamins, antioxidants and other healthful compounds. The protein of eggs is considered as highest quality protein as compared to other animal protein sources which is readily available and most inexpensive. Eggs are good source of all well balanced nutrients for persons of all age groups. Egg is a unique combination nutrient like it is rich in protein and low in calorie. The nutrients concentration in eggs is dependent on breed, strain, species, feed, age, season, storage and processing methods. The nutritional value of eggs lies chiefly in their proteins of good biological value; an average-sized egg (60 g) provides approximately 7 g of proteins. These proteins are rich in essential amino acids with a very good balance between these amino acids. So the egg protein can be regarded as a reference protein. On comparison we can say that 2 eggs provide as much protein as 100 g of meat or 100 g of fish. The energy value for an average egg (60 g) is approximately 376 Kj (90 kcal). The lipid content is 7 g, most of these lipids being contained in the yolk. Among the total fatty acids 2/3rd of these are unsaturated. Egg also contains 180 mg of cholesterol and rich in vitamins (A, D, E) and trace elements (iron and zinc).

Meat and meat products industry is a sunrise industry in India having huge potential for upliftment of agricultural economy. India is the second largest food producer in world but the contribution of meat industry is still confined to localized handlers. So to uplift industry basic infrastructure, food chains, food security and safety along with standards and laws formulation and their implementations is today’s need. Standards are carefully drawn specifications with respect to a food product. These standards are mainly of three types i.e. government, trade and research standards. Government standards are formulated by the government of India or state government and also have the power to implement these standards while trade standards are implied by the individual company. Research standards are formulated on the basis of comparative study of the products by the researchers. Laws are the legislations enforced by the organisations either government or by autonomous bodies made under the constitutional acts of the country. At present numerous standards and laws are in existence in individual country or at international level. The need is to harmonize them as per the industry and trade requirement. Domestic Organizations Involved in Standards and Laws Formulation For the formulation, enforcement and control of standards and legislations at domestic level various organizations are of prime value. Some of these are:

Organic meat food products are choice products for health conscious people. These are the products produced mainly in most natural way without using fertilizers, pesticides and growth regulators in their feed and fodders. In addition, meat animals are also reared without growth hormones, antibiotics drugs etc. and grazing of the animals is a prerequisite in organic farming system. The term organic farming was first time used by Lord Northbourne. The term organic farming was derived from his concept of “the farm as organism”. It is a holistic, ecologically balanced approach to farming. In 1991, the European Commission formulated the first government system to regulate organic labelling. In India, standards for organic agriculture were announced in May 2001 and the National Programme on Organic Production (NPOP) is administered under the Ministry of Commerce. The concept of organic meat food products has been emphasized now a day due to animal welfare point of view, consumer’s conscious ness, less residue level of chemicals, antibiotics and drugs, Bovine spongiform encephalopathy (BSE), Foot and mouth disease (FMD), genetically modified food etc. Organic meat food products as a whole are dependent on the life stages of the livestock like its origin, feeding, housing, health, breeding, transportation and slaughter. The other factors accounting for organic foods are its production, harvesting, preservation and processing. Inspite of low growth rate of organic red meat globally, growth in dairy, egg and poultry is increasing day by day. At present 1.2 million hectares (0.6%) of Indian land is producing organic products (Willer and Kelcher, 2011).

Genetically modified organisms (GMOs) can be defined as organisms in which the genetic material (DNA) has been altered in a way that does not occur naturally. The technology is often called “modern biotechnology” or “gene technology”, sometimes also “recombinant DNA technology” or “genetic engineering”. It allows selected individual genes to be transferred from one organism into another, also between non-related species. Genetically modified (GM) foods are foods derived from genetically modified animals and marines. Genetically modified animals and marines are those in which specific changes introduced into their DNA by genetic engineering using a process of either Cisgenesis or Transgenesis. These techniques are much more precise than mutagenesis where animals and marines are exposed to radiation or chemicals to create a non-specific but stable change. Other techniques by which food animals can be modified are selective breeding and somaclonal variation. By this means desired quality of meat, egg and other animal products can be produced. But it is a common thinking that foods derived from genetically modified animals and marines are far from safe. They are likely to be contaminated by potent vaccines, immune regulators and growth hormones, as well as nucleic acids, viruses and bacteria that have the potential to create pathogens and to trigger cancer.

Index Symbol α–actinin 27, 31, 114 β-actinin 27, 32 A Acid meat 51, 339 Acid phosphatase 114 Actin 18, 19, 21-23, 27, 29-32, 44-46, 50, 52, 82, 114, 155, 237, 297 Active packaging 177 Actomyosin 30, 31, 45, 113 Adhesiveness 254 Adipose tissue 24, 27, 34, 35 Adrenaline 52