Preface In 1937, Dr. Benjamin Peary Pal, the arcitect of modern agriculture in India and a famous rosarian in his much quoted article sSearch for New genes” underlined a message…” The scientific breeding of cultivated plants has become indispensible in the agriculture of progressive countries in order to meet the changing and increasing demands for food and raw materials…. The rediscovery of Mendel’s law of heredity in 1900 gave a stimulus to plant breeding and numerous new forms of plants have been evolved by selection and hybridization…”. With the setting up of the plant introduction section in the erstwhile Division of Botany at IARI, marked the beginning of systematic plant exploration and introduction in India. In the 1960’s, the International council of Scientific Unions founded the International Biological Program(IBP) whose one of the concerns was plant genetic resources and in 1967 jointly with FAO it first organized the first Technical Conference on Plant Genetic Resources. In 1974, CGIAR set up an International Board of Plant Genetic Resources(IBPGR). In 1985, FAO stepped in to establish the first commission on Plant Genetic Resources. Between 1988 and 1991, Plant Breeder’s Rights and Farmer’s Rights gained formal acceptance at the Keystone International Dialogue series on Plant Genetic Resources. Today, there is acceptance on recognizing and rewarding informal innovations that go into plant improvement programs for which conservation of plant genetic resources is absolute. Horticulture plays a key role by contributing a lot towards our Agricultural GDP. Preserving it’s rich biodiversity is a key contributor in achieving the set target. Today this biodiversity meets and stimulates the various requirements of the market (in terms of new types of product, quality standard, and rediscovery of traditions), of the production sector (in terms of plants more adaptable to climate change, new cultivation methods and cultivation environments or biotic/abiotic stresses) as well as the needs of the processing industry and of modern distribution This book encompasses on subjects like Biodiversity of vegetable crops; Efficient management of plant genetic resources through use of core collections; Collection, characterization and conservation of indigenous germplasm; In-situ conservation approaches; IPR in relation to germplasm management ; GM crops-their bio safety issues and regulation; bio-informatics tools for diversity analysis; biochemical characterization and tolerance mechanism to cold stress. Full scope of discussion on various aspects is beyond the ambit of this book , however the editors and authors have tried their best to bring in most of the facts and recent findings on the discussed chapters in a lucid manner. We owe to the authors of all those publications and literature whose help has been taken in organizing this book. We will be pleased to have suggestions for further improvement of the text.

Plant biodiversity is the single greatest resource that man garnered from nature during the long cultural development phase. More than 2.5 lakh species of mosses, ferns, conifers and flowering plants and more than 50,000 plant species are yet to be documented, primarily in the remote and less studied reaches of tropical forests. Vegetable crops in which rich diversity occurs in India are Cowpea, Common bean, Cole crops, Okra, Brinjal, Sweet potato, Taros, Yams, Sword bean, Velvet bean and Elephant foot yam. Wide diversity was observed in chives, leeks and other Allium spp in Kumaoun and Garhwal Himalayas; cluster bean in Western arid zone; lab lab bean in Deccan plateau; cucurbits in Rajasthan and Madhya Pradesh and leaf vegetables like Amaranth and Fagopyrum in Western Himalayan region. Tropical vegetables consist of solanaceus fruits (tomato, hot pepper, brinjal), cucurbits (water melon, muskmelon, cucumber, pumpkins and squashes, bottle gourd, chow-chow, ivy gourd, bitter gourd, ash gourd, round gourd, snake gourd, pointed gourd, sponge gourd, ridge gourd), malvaceous vegetables (Okra),legumes (common bean, lima bean, dolichos bean, cowpea, sword bean, winged bean), allium (onion, shallot, garlic, leek) and leaf vegetables (amaranth, spinach beet, Ceylon spinach). The indigenous vegetables both cultivated and wild have important role in household nutrition, economy and food security. There are 25 hot spots of biodiversity of which Western Ghats and Eastern Himalayan region are the most important ones. Indo-Myanmar region is one of the largest hot spots of the world and is the centre of origin of tuber crops like yams, taro, Amorphophallus and Alocasia. Tropical tuber crops form an important group of subsistence crops especially to those living in tropics and sub tropical zones. Tuber crops are the third important food crops after cereals and grain legumes. Major tuber crops are cassava, sweet potato, Asiatic yam, African yam, lesser yam, elephant foot yam, taro, tannia and country potato. Minor tuber crops are arrowroot, winged bean, yam bean, canna, safed musli and Jerusalem artichoke. Sweet potato is one of the world’s highest yielding crops. Biodiversity is the origin of all species of crops and domesticated and the variety within them. It is also the foundation of ecosystem services essential to sustain agriculture and human well-being. Today’s crop and livestock biodiversity are the result of many thousands years of human intervention. The biodiversity in vegetable crops is composed by the genetic diversity, as species diversity (inter specific diversity) and as diversity of genes within a species (intra specific diversity) referring to the vegetable grown varieties, and by the diversity of agro-ecosystems (agro-biodiversity). Crop diversity is the variance in genetic and phenotypic characteristics of plants used in agriculture. Over the past 50 years, there has been a major decline in two components of crop diversity; genetic diversity within each crop and the number of species commonly grown.

  Plant genetic resources represent both the basis for agricultural development and a reservoir of genetic adaptability that acts as a buffer against environmental changes. The erosion of these resources threatens world food security. From an agricultural utilitarian standpoint, plant genetic resources can be considered limited and perishable natural resources. PGR forms a distinct group and include all plant species, which contribute to people livelihoods by providing food, medicine, feed for domestic animals, fibres, clothing, shelter, energy and other uses and are subject to cultivation by man as well as the wild relatives of these species. Genetic diversity simply means all the variety of genes that exist in a particular variety or species. They provide the raw material (genes) which, when used and combined in the right way, produce new and better plant varieties, and are an irreplaceable source of such characteristics as resistance to disease, local adaptation, and productivity. Plant genetic resources are now, and will continue to be in the future, of inestimable value independently of whether scientists use them by means of conventional plant breeding or modern genetic engineering. These genes are dispersed throughout local cultivars and natural plant populations that have been selected over thousands of years by farmers and nature for their characteristics of adaptation, resistance and/or productivity. The road to a continuous increase in the production and quality of food lies through the protection and efficient utilization of plant genetic resources; this requires their conservation, evaluation, documentation and exchange.

PLANT EXPLORATION AND GERMPLASM COLLECTION OF PLANT GENETIC RESOURCES Plant exploration and collection activities have been undertaken for a wide range of needs - by naturalists, travelers and plant hunters over the past several centuries in the quest for new and better economic plants in the southeast Asian region, ornamentals in the Himalayas and many others; by taxonomists for working out plant diversity and delineating phyto- geographic differences, and providing a basis for pinpointing areas with high species diversity and changes over time; world-wide collection, augmentation and characterization of crop plant diversity by Vavilov and co-workers (1926) for understanding the patterns of crop plant diversity and delineating centres of diversity; search for wild forms of species, and wild relatives of crop plants for working out primary centres of origin and crop genepools; biosystematic study, survey and in situ study and collection in protected areas for determining patterns of diversity build-up in crop and wild species. Concept of centres of origin of crop plants, importance of the wild genepool of crops, dependence on wild plants as a source of edible and otherwise useful species ensuing from these efforts provide the basis for systematic exploration and collection of plant genetic resources.

The Indian centre of origin and diversity of cultivated plants represent an outstandingly rich reservoir of nature’s wealth of plant species which include a large number of economically important species including Vegetable Crops. Common vegetables like okra and brinjal are native to this region. Out of the recorded 110 genera and 640 species of cucurbits, 100 species representing 36 genera occur in India. Twenty of these species are endemic. Adoption of high yielding crop varieties and hybrids along with changing land use patterns have led to rapid replacement of native cultivars in most cases. Conserving and sustainable utilization of locally adapted types and primitive landraces are, hence, now major concerns at the national and global levels. The National Bureau of Plant Genetic Resources (NBPGR) at New Delhi is the nodal organization in India with the national mandate to plan, conduct, promote and coordinate all activities concerning plant exploration and collection, germ plasm import and exchange, plant quarantine, germplasm multiplication and distribution, evaluation and characterization, documentation and conservation of both indigenous and introduced genetic variability in cultivated plants and their wild relatives. The challenging job of collecting, genetic evaluation and utilization of plant genetic resources cannot be satisfactorily accomplished by one organization. The enormous task of safeguarding and promoting greater utilization of these valuable resources can be best achieved through a networking approach. Professional development is the key to success of this endeavour. NBPGR organizes regularly short-term training courses on various aspects of PGR to impart latest knowledge and techniques to scientists and technicians engaged in crop improvement programmes and handling germpalsm collections.

Intellectual property: It can be defined as creations of intellectual brain which has value, utility/application in the society and can be traded in commerce for economic benefits. Significance of Intellectual Property: The significance of protection of Intellectual Property has been realized for wealth and value creation in a knowledge-based economy. The agricultural scientists are comparatively little aware of IPR issues because their primary mandate has always been the free delivery of technology to the end users particularly the farmers. After the implementation of TRIPS Agreement during 1995, the worldwide focus of scientists and planners was shifted on the development of time appropriate technologies, protection of inventions/ technologies and real time technology transfer through licensing for industrial and agricultural growth leading to overall economic development. Under the changing scenario of global trade, where national governments are entering into bilateral agreements for tackling IPR issues to promote trade, agricultural scientists must also look for managing their intellectual properties in tune with the existing IP legislation of the country.

INTRODUCTION Genetic engineering (GE) and genetically modified organisms (GMOs) provide powerful tools for sustainable development in agriculture, healthcare and many other industries. GMOs are those which were genetically engineered in a laboratory by incorporating a small foreign DNA fragment carrying a gene of interest into the native DNA of the organism. The foreign gene is attached with the necessary regulatory elements to help its expression in the new genetic environment. Genetically modified (GM) crops currently account for some 16 percent of the world’s land planted with soybean, maize, cotton and canola. The global demand for food is increasing because of the growing world population and decreasing arable land. At the same time food and agricultural systems have to respond to several changes such as increasing international competition, globalization and rising consumer demands for improved food quality, safety, health enhancement and convenience. Modern biotechnology involving the use of rDNA technology/genetic engineering has emerged as a powerful tool with many potential application for improving the quantity and quality of food supply. Foods derived from genetically modified crops, commonly referred to as genetically modified food and food ingredients have already become available worldwide with aim of enhancing productivity, decreasing the use of certain agricultural chemicals, modifying the inherent properties of crops, improving the nutritional value or even increasing shelf life. Results of DNA modification may not be limited only to the particular characteristics of the replaced gene. It is therefore important to ensure that when these organisms are released into nature they do not harm the environment or human health [EFSA, 2006]. Such concerns have led to broader interests in the theme of risk assessment in the release of GMOs. A cautious approach is necessary to assess environmental risks which may occur due to introduction of recombinant organisms in the natural environment [Johnson et al., 2007].

Genetic diversity is one of the fundamental character present in any population or species. It is the total variation either phenotypic or genotypic which have occured in a population through due course of evolution. Variation in any species depends on mutation, substitution (insertion/deletion) and response to selection. However, comprehensive studies on theoretical and empirical perspectives for diversity analysis may be found by evolutionary biologists to understand the factor that influences levels of genetic diversity in natural population (Lewontin, 1974). In brief, nucleotides/ protein codes are the basic unit to quantify total amount of variation. Bioinformatic tools and techniques help us to measure inferring relationships between genes, proteins or organisms through use of these DNA/protein sequences by integration of mathematical algorithms, statistical and computer databases. Diversity analysis is popularly known as phylogenetic analysis in bioinformatic terms. The basic principle of phylogeny is analysis/alignment of characters; whether it is morphological or molecular data. For instance, in case of morphological data of a crop species, the leaf colour/stem colour/fruit colour/grain size etc. may be compared however sequence data is problem of interest for molecular phylogeny. The evolutionary history of any crop species/ organisms inferred from phylogeny analysis mostly depicted through treelike diagrams/ dendrograms. In this chapter, phylogenetic analysis based on only nucleotide/protein sequences are discussed. For analysis of phylogenetics, default assumptions (Baxevanis and Ouellette, 2006) are:

Vegetable crops, known as protective foods, are important for the healthy diet providing vitamins, minerals, fibre, amino acids and other bioactive compounds. Improvement in vegetable crops is inevitable for better yield, pest or disease resistance, morphological characters, nutritive quality, to adapt with environmental stress, climate change, offseason production, satisfying varying consumer preference etc. Vegetable crops comprise of a large number of species and are rich in diversity. Diverse genetic resources are pre-requisite for any crop improvement programme and vegetable improvement is carried out using exotic introductions, indigenous collection and hybrids/varieties by different breeding systems. Characterisation of genotypes is the description of germplasm giving information on heritable characters so that promising one can be utilised in breeding programme which is the best option for higher yield and other qualities. The morphological, physiological and molecular (biochemical and DNA) traits are called qualitative descriptor and yield, pest/disease resistance, stress tolerance etc are quantitative descriptors that are easily influenced by the environment factors. Apart from the higher production and resistance to biotic and abiotic factors, nutritional traits, flavour, processing qualities that increase the food value is also important in improvement programme of fruits as well as vegetables. Genetic resources of vegetables available at international and national levels are characterised, evaluated and conserved. With the high diversity of vegetable crops, characterisation and evaluation of genetic resources become a stupendous responsibility requiring extensive infrastructure facilities and varying methods of evaluation (Srivastva, 2012). Genetic resources are characterised on highly heritable traits expressed across the environment. Apart from morphological characterisation, biochemical and molecular characterisation is also conducted that are useful for the identification of biotic and abiotic stress parameters, nutritional and processing quality. Genotype characterisation can be done efficiently using potential of biochemical and molecular markers that help to set up the breeding programme.

Among abiotic stresses, low and high temperature stress is very critical for determining the agricultural production. Plants exhibit a maximum rate of growth and development at an optimum temperature or over a diurnal range of temperatures (Fitter and Hay, 1981). When ambient temperature deviates from optimal, physiological, biochemical, metabolic and molecular changes occur within plants. This is an effort of plants to maximize growth and developmental processes and to maintain cellular homeostasis during such adverse conditions. Under increasingly stressful conditions, plants experience progressively more abnormal, impaired or dysfunctional cellular and whole-plant processes until the cardinal temperatures for survival are reached (Fitter and Hay, 1981). At the extremes of the natural temperature range of a plant, the degree of physiological, cellular, metabolic and molecular dysfunction becomes so severe that it leads to death. Growth constraints and stress result in significant crop losses and therefore the mechanisms underlying endurance and adaptation to these changes have long been the focus of intense research (Bray 2004). Kültz (2005) elaborated two types of responses to a particular kind of stress: