Seed is associated with human civilization and culture and played important role in the successful crop production and sustaining prosperous agriculture. The philosophy based on the virtues of the seed has eulogized the seed as the important initial capital and the germinating seed as the real indicator of the success of the crop and the quality seed itself ensuring half the success of the crop. Seed is a living thing. Maintenance of genetic characteristics in self pollinated and cross pollinated crops through seeds demand well defined procedures. The quality assured seeds are the resultant products of breeding improved crop varieties and clonal crops and helped the mankind from food scarcity to achieve food security and added nutritional security as well. Quality assured seeds of the improved crop helped to defend environmental and climate challenges and biotic and abiotic stresses.

India had fourfold increase in population from 0.3 billion to 1.34 billion and registered a fivefold increase in food grain production from 50 million tons to 285 million tons in 2018 (Paroda, 2020). 

1. Seed is the key ingredient in establishment and expansion, diversification and improvement of crops/and crop husbandry. 2. Seed is the most effective and efficient means of crop propagation.

Genetic purity is the vital determinant in continuing the genetic integrity in seed from generation to generation. This is the basis of variety purity maintenance. The genetic purity depends upon the purity of the seeds planted. The genetic purity of the seeds planted must exceed or equal to the final product purity standards required as the purity generally decreases with subsequent generations of propagation. The improved varieties loose their identity and healthiness unless properly maintained. Maintenance selection can prevent the seed quality deterioration.

Clonal crops belong to at least 34 families including herbs, shrubs, trees and vines. Clonal crop improvement dates back to 10,000 years ago. The diversity of clonal crops and their wild relatives are very significant but not yet fully understood and exploited. Many crops represent combination of sexual and clonal reproduction. Different parts of the clonal crops which may or may not be the same as consumed parts have been shaped as clonal propagules such as stem, setts, tuber, rhizome, corms and bulbs among others.

Micrografting is a method to produce virus free, viroid free and disease free grafts that are clonally propagated. This method is an aid to mass multiplication. In vitro grafting is applied to difficult to root clones. Micrografting is performed to understand incompatibility prediction between the two grafting partners, virus indexing, deduction of latent virus, safe germplasm exchange and to do grafting functions throughout the year. It is not season specific. Protocols for micrografting are available for almond, pistachia, olive, prunus, grape, mulberry, cashew, citrus, peach, pear, avocado, hazlenut, walnut, chestnut and grape fruit (Hussein et al., 2014).

In vitro cloning is a vegetative propagation performed in the Plant Tissue Culture system. In vitro micropropagation cloning propagation is mostly applied to the vegetatively propagated plants and in particular in ornamental, fruit and in a few orchard crops and in vegetable crops besides in many forest tree species.

Organically produced seeds are obtained by adapting the organic crop production system. Organic seed is a critical input but specialised efforts to produce organic seeds are not adequately and systematically followed. Accredited organic farming standards recognises the organic seed production as a prerequisite to organic farming. Seed health is important in organic seeds since seeds are not treated with chemical fungicides.

Synthetic seeds are also known as somatic seeds, artificial seeds, clonal seeds and synseeds. They are analogue for botanical seeds. Somatic embryoids which are tissue culture produced embyoids with bipolar structures, apical and basal meristematic regions which are capable of forming shoot and root respectively. Somatic embryoids are encapsulated and are used as artificial seeds possessing the ability to convert into plants in in vitro and ex vitro conditions. Artificial seeds are broadly two types.

Crop improvement is a continuous process. Crop improvement aims to genetically enhance, enrich and empower the crop plants with superior performance, enhanced yield level, higher quality of food, nutritional, fodder, energy produce and adaptability to climate change and crop growing environments. The intrinsic virtues of the improved cultivars could be sustained mainly through the production of the quality assured seeds endowed with genetic capabilities. The quality assured seed delivery system is through the seed chain from the basic seeds through Nucleus-Breeder-Foundation-Certified seed. Quality seed production system need to be based on genetic, breeding and seed technological protocols including field and laboratory testing to ensure very high genetic purity, true to type and superiority.

Deterioration of Crop varieties in their genetic endowments/qualities has been explained by Kadam (1942). Publication on maintenance and management of breeder seed production (Laverack 1991), and maintennce of self pollinated crop varieies (De Hanns 1953; Parlevliet 2007) explain in detail various aspects in maintenance of the seed quality.

Molecular markers may be a short DNA sequence that flanks base pairs like SNP, a Single Nucleotude Polymorphism, small fragment or SSR Single Sequence Repeat or an extended site as in the case of microsatellites. Schematic representation of the SSR assay is shown in Fig. 1. Genetic markers have to be easily identified, linked to a specific locus and polymorphic. Molecular markers are subdivided into codominant monolocus and polyallelic markers and the second group contains dominant, polyallelic and biallelic markers. 

Leaves are alternate in distichous arrangement; lamina long and linear and leaf sheath covering the internodes; ligule membranous and auricle claw shaped. The inflorescence is a spike and florets are borne on rachis node. Each spikelet has three florets. In two row barely, lateral spikelets become sterile.

Agathi is a fast growing tree upto 10m. tall. Branching is drooping. The stem bark is deeply furrowing forming cork like plates. Leaves are compound, pinnae, 20 to 30 cm. long glabresence to pubescent. Leaflet oblong rounded 18 to 24 pairs of leaflets that are green to dark green mostly, bitter free. Inflorescence is a raceme 4 to 7 cm long and bearing 2 to 5 flowers that are white, red, yellowish and pink. Flowers are 5 to 10 cm long, 3cm wide, curved calyx bell shaped 2 cm long, 2 lobed, fine teeth like serration, fleshy. 

Conservation of released varieties requires a sound scientific and technical basis. There are two main strategies for conserving agricultural biodiversity, namely ex-situ and in-situ conservation, both of which are equally important. In-situ conservation refers to the maintenance of a species in its natural habitat. Exsitu conservation is the conservation of components of biodiversity outside their natural habitats. It is conserved in the form of seeds (seed gene bank), live plants (field gene bank), tissues (tissue gene bank), pollens (pollen gene bank) and DNA materials (DNA bank). 

In order to provide for the establishment of an effective system for protection of plant varieties, the rights of farmers and plant breeders and to encourage the development of new varieties of plants it has been considered necessary to recognize and protect the rights of the farmers in respect of their contribution made at any time in conserving, improving and making available plant genetic resources for the development of the new plant varieties. Moreover to accelerate agricultural development, it is necessary to protect plants breeders’ rights to stimulate investment for research and development for the development of new plant varieties.

Adhikari, S., Bandoupadyay, T., Ghosh P. (2014). Assessment of genetic stability of Cucumis sativus regenerated from encapsulated shoot tip. Sci. Horti. 170: 115-122. Archana C.P., Deepu, V. Geetha S.P. and Indira B. (2013). RAPD assessment for identification of clonal fidelity of micro rhizome induced plants in Turmeric (Curcuma longa) CVs. Internat. Food Res J: 20: 3325-3328.