eChapters

Introduction

Once the cashew fruits fallen, are collected, the nuts are to be separated from the cashew apple and sun dried for 2 to 3 days. This is very important in view of the quality of kernels obtained during subsequent processing. Raw nuts at the time of harvest will have moisture content ranging between 16 to 25 per cent; while sun dried nuts have moisture content of 8 to 10 per cent (Nagaraja, 2000). If the nuts are not dried and stored properly, it leads to fungal spoilage resulting in poor quality of kernels. Fungi such as Gonatobotryum, Helminthosporium sp., Corynespora sp., Alteraaria sp., Verticillium sp. and many species of Aspergillus have been isolated from stored cashew nuts (Joseph, 1981). In view of the occurrence of fungi, drying of the raw nuts before storage becomes absolutely essential. Maximum permissible moisture content of raw cashew nuts is 8.7 to 9.1% (Ohler, 1979). Okwelogu and Mackay (1969) have reported that high whole nut or kernel moisture percentages are closely associated with high shell moisture contents and for nuts whose moisture content is in equilibrium with the ambient relative humidity, the moisture content of whole nut and kernel could be predicted from that of shell with more than 99% accuracy. A higher kernel shell weight ratio has been shown to be inversely proportional to moisture content.

Abstract

The runners of ten strawberry cultivars were procured from different research stations, planted in the last week of October every year and mulched with paddy straw.  The earliest flowering was observed in Chandler, which produced the largest sized fruits (13.20 g) followedby Dana.(3.6 g). Higher incidence of fruit rot was observed in Chandler (26.2%) and least in the Sweet Charlie (9.3%). Sweet Charlie produced medium sized (11.80 g), highly glossy and attractive fruits having high TSS (10.2%), and minimum fruit rot can be better substitute for Chandler in sub-tropical areas.

Several cryogenic agents have been used in many countries for freezing and preservation of bovine semen like solid carbon-di-oxide (-79°C), liquid air (-183°C), liquid helium (-268°C) and liquid nitrogen (-196°C).  Liquid nitrogen is 4th coldest substance known and its small amount is converted into a very large amount (near about 700 times) of its volume when exposed to atmosphere.  It is a colourless, odourless, non-irritant, non-toxic, non-caustic, non-corrosive, non-inflammatory and inert gas having boiling point -196°C temperature.  Being a liquid it comes into good contact with the surface of the semen straws and constant storage temperature is maintained throughout the straw and due to these properties LN2 is most widely used refrigerant in the world.

Autoclave Operating Procedures

Place all material to be autoclaved in an autoclavable tray. All items should have indicator tape. Separate liquids and solids should be autoclaved separately. Ensure that the lids on all bottles are loose. Do not put a large number of items in tray, to ensure that all items reach the appropriate temperature. Also allow sufficient air/steam circulation.

Steps in autoclave

Pressure should read ‘O’ when you open the pressure chamber of the autoclave.

Place items to be autoclaved in the autoclave and close the door. Some autoclaves require that you also lock the door after it’s closed.

Set time - typically 20 minutes.

Temperature should be set at 121°C already, but double-check and change if necessary.

Set cycle: If liquid, set “liquid cycle” or “slow exhaust”. If dry, set “dry cycle” or “fast exhaust” + dry time.

Start the cycle. On some autoclaves, the cycle starts automatically at step 5. On others, turn to “sterilize”

Pesticide Toxicity

Chemical tactics to manage pests can include many types of compounds. Some merely repel or confuse pests. Some interfere with weed photosynthesis, insect molting processes or development in some way. Others, including some botanical and most conventional insecticides are broadly toxic to living systems. The term pesticide literally means “pest killer". Generally speaking, when using pesticides, we are trying to garner the advantages of using pesticides while minimizing the risk to humans and other non-target organisms. Risk to humans of pesticide use is often described as:

An area with similar soil, climatic conditions and resource availability resulting in similar type of crops and cropping systems. Ex. Rice based cropping system, Ragi based cropping system, Wheat based cropping system, Cotton based cropping system etc.

Efficient cropping zone: An analysis of productivity efficiency of crop was made by Kanwar (1972) using relative yield index (RYI) and relative spread index (RSI).

A zone where both yield and spread indices are maximum that is above 90 per cent is called most efficient and in case of indices below 90 is the least efficient zone of a crop.

1. Botanical Name : Madhuca longifolia var. latifolia (Rox.) A. Chev.

2. Family : Sapotaceae

3. Common Name : Indian Butter Tree

4. Name in Indian languages

Mahua, Mohwa (Hindi), Maul (Bengali), Kat-Illipi (Marathi), Illupa Malayalam), Ippa (Telugu), Mahudo (Gujarati), Hippe Mara (Kannada).

5. Description

Indian Butter tree (mahua) is one of the most important trees of Indian forest. It produces delicious and nutritive flowers, fruits, seeds and valuable timber. It is a deciduous tree with a large, spreading, rounded crown that can grow 16 metres or more tall. The short main trunk can be up to 80 cm in diameter. Mahua is an indigenous tree of high economic value. A characteristic tree of the dry region, it is found in north and central India. The tree is very well known to rural folk since ages in India. Its flowers, fruits, and oil obtained from seeds are eaten in various ways. Mahua flowers are a rich source of sugar containing appreciable amount of vitamins and minerals. Fruit is eaten as raw or cooked. Pulp is a good source of sugar, whereas dry husk makes a good source of absolute alcohol. Seed is a good source of oil.

Planting System

Main objective of layout is maximum number of plant accommodated in per unit area without affecting the efficiency of production. If more than one fruit is included in the orchard plan, each kind should be grouped into individual block. Similarly fruit ripened in particular season should be grouped to facilitate convenience in watch and ward, harvest and post harvest handling. Some kind of fruit trees need pollinators/pollinisers for improving the fruit set.

An ideotype is a crop plant having desirable morphological and physiological characteristics or both for cultivation in a specific agroclimate situation. An ideotype is constructed by the breeder on the basis of indepth study of morphological and physiological characters including their interrelationships, and intuition of the breeder based on his experience.

In the flesh of the fruit, tiny, 2-10 mm (depending on the cultivar) brown blemishes form. Below the surface, the tissue gets black and corky. At harvest or after cold storage, the skin develops depressed patches on the surface. These tend to develop as water-soaked patches on the skin around the calyx. Overall, these patches became darker and sunken than the skin around and developed entirely after one to two months in storage.

Abstract

PCR primer design is a crucial skill for molecular biologists, as it enables the study of gene expression, gene amplification, and molecular markers. Primer3 is an online tool widely used for designing optimal PCR primers, which are pairs of short oligonucleotides that initiate DNA synthesis in vitro using a thermocycler. This tool uses a range of parameters to generate high- quality primers, including target sequence length, melting temperature, GC content, specificity, and product size. Understanding and effectively utilizing these parameters is essential for achieving accurate and reliable PCR results. In this chapter, the practical aspect of primer designing using the online tool Primer3 has been discussed. The parameters and the purpose of their usage have been given in a tabulated manner.

Abstract

A promising strain/seedling have been identified that produces only male flowers (small and pink tinged) on the ventral side of the current season’s shoot all along its length. As many as 24 flowers per shoot were observed. The seedling continued to flower for about one month which attracts large number of honey bees. The pollen is also produced in abundance that dehisces early in the morning. Preliminary observations on the flowering behaviour and pollen studies have revealed that it can be used as a suitable polliniser for persimmon cultivars in general and Hachiya cultivar in particular.

Introduction

Substances found in many plants are generally related to one of the phenols, pyrogallol or catechol. Tannins are solids, usually extracted by hot water insoluble in ether, chloroform, carbon disulphide, benzene; soluble in alcohol ether mixture, alcohol, water and in ethyl acetate, possessing a bitter astringent taste.

Tannic acid is Pentadigalloyl glucose, on hydrolysis yields digallic acid and glucose. Tannins are water soluble glucosides of polydepsides, polymeric ester of gallic acid. It is hydrolysed enzymatically and some efforts have, therefore, been made to preserve tannic, if it is to exert its activity in the duodenum. On the basis of physical or chemical principles which they employ, the methods of tannin analysis  can be grouped as follows.

The various steps involved in frozen semen technology are vital as the quality of semen determines the conception rate.  The key factor enabling the successful long term storage of frozen spermatozoa is maintenance at ultra low temperature.  Effective semen storage depends heavily on good identification and stock control.  Frozen semen can be easily damaged in the process of repeated handling to establish identity.

Bone section method

Advantage

Recommended for artificial limb fitting.

Non-weight bearing of stump is marked. Fig- 2a.

Storage

Nucleic acids are very sensitive to contaminations hence care should be taken while processing and storing DNA and RNA. The following properties of reagents and conditions need to be taken care of:

Heavy metals cause breakage of phosphodiester linkage. One can use EDTA which is an excellent heavy metal chelator.

UV rays cause extensive damage to nucleic acids. UV light at 260 nm causes breakage of thymine dimers and cross-link, including loss of biological activities.

Ethidium bromide results in photo oxidation of DNA. Oxidation products can cause phosphodiester breakage.

Nucleases are found on human skin; therefore, it is advisable to avoid direct or indirect contact between nucleic acids and fingers.

Most DNAes are not very stable; however, many RNAses are very stable and can absorb to glass or plastic and remain active.

At -20°C: extensive single and double strand breaks results. Hence for long-term storage it should be stores at -70°C. ? It is best to store DNA in high salt ( >1M) in the presence of high EDTA (>10mM) at pH 8.5, for long-term storage.

Storage of DNA in CsCl with ethidium bromide in the dark is an excellent storage medium. There is about one phosphodiester break per 200 kb of DNA per year.

Storage of ë DNA in the phage is better than storing the pure DNA.

1. Botanical Name : Meyna laxiflora Robyns

2. Family : Rubiaceae

3. Common Name : Moina, Aliv

4. Name in Indian languages

Kutkura, Moin (Assamese), Mainphal, Muduna, Muyna, Muyuna (Bengal), Alu, Atu (Gujrati), Moina, Muduna, Muyuna (Hindi), Mullakare, Gundkare, gobergally (Kannada), Alu, Huloo, Halawni (Marathi), Pindi, Pinditaka, Pindituka, Pindu (Sanskirit), Manakkarai (Tamil), Segagadda, Veliki, Visikilamu (Telgu), Monono, Montaphoo (Odiya).

5. Description

It is a large common shrub or a small tree and commonly found in evergreen forests. Meyna is a genus of shrub and small trees distributed in tropical and subtropical region. The fruits are used for preparation of pickle.

13.1. Types

Lamps: Head lamp, plough lamp, indicators

Switches: Safety switch, starter switch, etc

13.2. Components

Battery: A battery provides power to electrical or electronic circuits. The voltage rating of a battery tells how much electrical “pressure” the battery can provide. Battery energy is used to operate starter motor and to provide current to the ignition system during cranking. The electrolyte should always be maintained just below the maximum level, but should not go down below the 1/4^th level.

Alternator: Alternators are electromechanical devices used to convert mechanical energy to alternating current electrical energy. While some of the alternators use rotating magnetic field, some use linear. The functioning of alternators is similar to that of direct current generators. As the magnetic field around a conductor changes, a current is induced in the conductor. The rotating magnet or the rotor turns and cuts the field across the conductors. Thus, an electrical current is produced which causes the rotor to turn. This rotating magnetic field causes an AC voltage in the stator windings. In tractors, alternators are used to charge the battery and to power the electric system when its engine is running. An alternator is superior to a dynamo as it provides more precise output control, requires lesser maintenance, is more compact and requires no cut-out.

The extension/research work organization consisted of seven distinct steps namely,

Situation analysis

Identification of problems and setting up of objectives

Designing of interventions

Preparing plan of work & implementation of project interventions

Monitoring of implementation process

Impact assessment

Project reconsideration

In grape growing areas in many parts of the world this illness is a very popular disorder. In this disorder, a bold beer is surrounded by shoot berries, and the name of the hen and of the chicken disorder is like a hen, as bold berry and chicken. The fruit has a sour taste. The signs are the death of rising tips, falling leaves and the fragility of new shoots. With the development of interveinal chlorosis between the edges, the leaves may be deformed, and this is particularly evident after fruiting.