eChapters

The oesophageal groove (also known as the reticular groove) is a physiological structure and reflex in ruminants that plays a crucial role in the digestion of milk and liquids, particularly in young animals. Its significance and the timing of its closure vary across different species of animals, reflecting adaptations to their feeding behaviors, nutritional needs, and developmental stages.
Anatomy and function of the oesophageal groove
The oesophageal groove is a muscular fold located in the reticulum, the first compartment of the ruminant stomach. When activated, it forms a channel that directs ingested liquids (e.g., milk in young animals) directly to the omasum and abomasum, bypassing the rumen. This mechanism prevents milk from fermenting in the rumen, ensuring efficient digestion and nutrient absorption. The closure of the groove is triggered by specific stimuli, such as suckling, milk temperature, and sensory cues like the smell or taste of milk.
Species-specific significance and closure time
The significance and closure time of the oesophageal groove differ among species, influenced by their physiological needs and feeding practices.
1. Cattle (Bos taurus)
Significance: In calves, the oesophageal groove is critical for survival during the milk-feeding stage. By directing milk to the abomasum (the true stomach), it avoids fermentation in the rumen, which can lead to bloating and metabolic disturbances.
Closure time: The reflex is most active in calves and begins to diminish as they transition to a diet of solid feed, usually around 8–12 weeks of age. In adult cattle, the reflex is rarely triggered, although specific
stimuli (e.g., salty solutions) can induce it.

Abstract

Sabatayn basin is one of the two mature basins producing oil and gas in Yemen. Due to the recent decline in oil production, the exploration activities have been extensively concentrated mainly on these two basins (Sabatayn and Sayun-Masilah). So, this present study has been conducted to evaluate the exploration potential of the remaining trap areas within these mature basins. Fractured basement of reservoir becomes very attractive exploration target in Yemen recently, after the recent discoveries of basement fractured oil fields. As the evaluation of basement traps needs time and fund, to reduce the same, this research study uses a new technique which was devised with the help of Geoinformatics by analyzing the surface and subsurface lineaments effectively. In this study, a satellite image Enhanced Thematic Mapper (ETM+7) based analysis was conducted for extracting surface lineaments, and for the subsurface lineaments, the basement lineaments were extracted from seismic, magnetic and gravity data. An orientation analysis of these surface and subsurface linear features was performed to detect the basic structural grains of the study area. The correlation between these structural grains and subsurface oil and gas traps was performed to understand the connectivity to the reservoirs. This paper discusses in detail about the same and the importance of using surface and subsurface lineament analysis for delineating hydrocarbon reservoirs in the study area.

Introduction

Synbiotics refer to nutritional supplements combining probiotics and prebiotics in form of a synergism. The synbiotic concept was first introduced as “mixtures of probiotics and prebiotics that beneficially affect the host by improving the survival and implantation of health-promoting bacteria. Probiotics are live bacteria which are intended to colonize the large intestine and confer physiological health benefits to the host. A prebiotic is a food or dietary supplement product that enhances the activity of probiotics. Synbiotics are not drugs and the effects are due to changes in bacteria population or enhancement of activity of the bacteria. A synbiotic may contain fiber, but all fiber is not necessarily a synbiotic.

Using prebiotics and probiotics in combination is often described as synbiotic, but the United Nations Food & Agriculture Organization (FAO) recommends that the term “synbiotic” be used only if the net health benefit is synergistic. A synbiotic contains fiber such as fructose oligosaccharide, galactose oligosaccharide, etc. and is intended to stimulate the microflora in the large intestine. The combination of probiotics and prebiotics work separately in the small and large intestine, but synergistically increase the overall gut health. Prebiotic has shown to increase the population and/or function of the probiotic it is paired with, as the probiotic is an external species, whereas prebiotics stimulate the flora which is already present.

Orthopaedic Radiography
 
Radiography as an aid to diagnosis the orthopaedic cases has become an integral part of every modern hospital. This technique may also be helpful in detecting some previously unrecognized lesions in the area radiographed.

The radiologist must be aware of the radiological anatomy of part being examined, and of its normal variation with age, species and breed. The anatomical features which can be appreciated in a radiograph are:

Silage fermentation is a dynamic process that is affected by variety of factors. The primary goal of making silage is to maximize the preservation of original nutrients in the forage crop for feeding at a later date. Unfortunately, fermentation in the silo is a much uncontrolled process usually leading to less than optimal preservation of nutrients. In order to assist in the fermentation process, various silage additives have been used to improve the nutrient and energy recovery in silage, often with subsequent improvements in animal performance (Henderson, 1993; Bolsen et al., 1996; Muck and Kung, 1997; Kung and Muck, 1997; Muhlbach, 2000). Their main functions are to either increase nutritional value of silage or improve fermentation so that storage losses are reduced. The benefits obtained from silage additives depend upon their influence on the silage fermentation process. These benefits are usually measured by the reduction in fermentation losses and/or improvement in silage quality and feeding value.

Ensiling is the term given for all physical and chemical changes that take place when forage with sufficient moisture content (60-65%) is stored in the absence of air for silage preservation. Silage fermentation is an exceptionally complex process involving biochemical interactions among the forage, microbial populations and the ensiling environment. Successful silage production depends upon the promotion of the fermentation brought by beneficial bacteria. The process is based on spontaneous lactic acid fermentation under anaerobic conditions. The lactic acid bacteria ferment the water-soluble carbohydrates in the crop to lactic acid, and to a lesser extent to acetic acid. Due to the production of these acids, the pH of the ensiled material decreases and spoilage micro-organisms are inhibited. The ideal characteristics of material for silage preservation are: DM content above 20% and an adequate level of fermentable substrate (8-10 percent of DM) in the form of water soluble carbohydrate. The ensiling material should also ideally have a physical form that allows easy compaction in the silo. Materials such as maize stover, sorghum stover and grass can be ensiled successfully, while crop residues such as rice and wheat straw, with low water soluble content, do not fulfill these requirements, and therefore pre-treatments, such as fine chopping or use of additives, or both, may be necessary.

Definition of silage: Silage refers to the product obtained when fresh, high-moisture green fodder undergoes fermentation in a controlled anaerobic environment. This process generates volatile fatty acids that help preserve the green material for an extended period.
Ensiling: The act of preserving green fodder as silage is termed ensiling
Silo: A silo is a sealed container designed for the preparation of silage. In India, pit silos are commonly used, typically measuring 2.4 to 3 meters in depth. For every cubic meter of space, approximately 400 kg of fodder can be accommodated, while a cubic foot can hold between 18 to 22 kg of fodder in a pit silo. Other types of silos include trench silos, bunker silos and tower silos. In rural areas, katcha silos are often constructed by excavating a pit or trench in the ground, with the walls and floor usually coated with a mixture of cow dung and clay in a 1:1 ratio.
Crops suitable for silage making: Crops that are high in soluble carbohydrates are ideal for silage production. Suitable options for ensiling include maize, sorghum, pearl millet, Napier grass and oats. Leguminous fodder crops are generally not suitable for ensiling unless they are combined with carbohydrate-rich fodders in a ratio of 1:3. The recommended ratio of green, succulent fodder to dry straw is 4:1 and the crops should contain approximately 30-35% dry matter at the time of ensiling. Hollow-stemmed plants are typically unsuitable for ensiling due to the air trapped within the stems; however, if the stems are trampled, they can yield good silage. To effectively ensile cultivated or natural grasses an addition of 3-3.5% molasses is necessary. Grasses and legumes present challenges for ensiling; grasses tend to be low in soluble carbohydrates, while legumes possess high moisture content, elevated protein levels and increased mineral content, which enhance their buffering capacity and resistance to pH fluctuations. Harvesting: The crop should be harvested between flowering and milk stage as they contain maximum amount of nutrients at that time. Crops with thick stems are suitable for silage making whereas thin stemmed crops are conserved for hay making.

It is recognized in developed countries that the production of silage of high quality cultivated forage can be a valuable component for the development of a high-performing and low-cost system of animal production, using a relatively low level of purchased concentrates. However, this appears inappropriate for smallholders in tropical countries, primarily due to lack or high cost, or both, of equipment for harvesting and conservation and production of cultivated forage being often limited due to lack of available land. Many agricultural, agro-industrial and fishery by-products have potential as animal feeds. Most farmers in developing countries rely for their food security on the cultivation of cereals, root crops and high-value crops such as fruits and vegetables, which understandably take priority in the allocation of land. Fruit, vegetables and root crops are increasingly integrated in the farming system and play a key role as staples in the human diet in most developing countries.

Silage is the green material produced by controlled anaerobic fermentation of the green succulent fodder crop retaining its moisture content. The process of preparing silage is known as Ensiling and the pit in which silage is prepared is called silo.

Crops suitable for silage making
The fundamental behind the conservation of fodder as silage is the acidic condition, which is produced by the anaerobic fermentation of carbohydrates rich fodder. Thus the necessity of the fodder crop being used for silage preparation is the presence of high amount of fermentable carbohydrates and the natural carbohydrate rich crops are the cereal crops like maize, jowar or bajra and grasses or their combination in 3:1 with leguminous crops. Otherwise high moisture leguminous crop after wilting in combination of molasses may be 
used.

Successful preservation of high-moisture forage and other crops depends upon the controlling the activities of microbes, particularly bacteria. Ensiling generally controls microbial activity by a combination of an anaerobic environment and a natural fermentation of sugars by lactic acid bacteria on the crop. The silage microflora plays a key role in the successful outcome of the conservation process. Therefore, it is important to understand both the microorganisms that are present on the crop at ensiling and how ensiling preserves the crop, inhibiting detrimental microorganisms. The crop at ensiling contains both aerobic and anaerobic microorganisms and a range of both bacteria and fungi that affect silage quality.

Silage is preserved pasture. Fresh forage crops such as maize, grasses, legumes, wheat and lucerne can be preserved by ensiling. Silage is the green material produced by controlled fermentation of the green fodder crop retaining the high moisture content, which contain 20-40% dry matter.

Silage can be prepared from plants having thick stems and are generally not very suitable for hay making like sorghum, maize. Weeds can also be utilized along with main fodder crops for silage making. Silage making kills majority of weed seeds. It is highly palatable. The organic acids produced in the silage are similar to those normally produced in the digestive tract of the ruminants and therefore are used in the same manner.

Estimation of silage quality parameters: Process of making silage is called ensiling. The green fodder harvested at a proper stage is stored, packed and compressed in silo and it is then tightly covered to prevent the contact with air. Thus, the forage is preserved by controlled microbial anaerobic fermentation in a silo with minimum loss of nutrients for use as a succulent fodder during scarcity (lean) period. This form of preserved fodder is known as silage. Silage is prepared by obtaining enough acid content in ensiled feed which inhibit the microbial fermentation thereby preserving the green fodder. Following are
points to ponder:
1. Maize, sorghum, bajra crops having thick solid stems and rich in soluble carbohydrates, are best for silage preparation. Silage can also be prepared from oats, berseem etcetera, after wilting to 35 – 40 % dry matter.
2. Dry matter content in fodder crop for ensiling should be between 30 – 40 % (average 35 %) and should have sufficient soluble sugars for acid production during fermentation.
3. The crop is harvested in bloom stage, which is best for silage preparation.
4. If the crop is deficient in soluble carbohydrates, (for example, legumes), then a sugar industry by – product is added.
5. Silage having acidic flavour and pH 3.5 to 4.2 is considered to be excellent.
6. The crop should be properly trampled / pressed to remove the air out of silo.
7. The crop is chopped into small pieces for better microbial action due to increased surface are and sufficient acid production.

Introduction
Antibiotic-resistant E. coli develops as a result of the heavy use of antibiotics in the production of food animals, which is then found in the meat from those animals. There have been reports of a number of antimicrobial resistant E. coli infections linked to the ingestion of tainted milk and chevon. As a result, the existence of antibiotic resistant E. coli is a significant and expanding global public health risk. Escherichia coli of animal origin has also been seen to colonise humans, and because it is resistant to many conventional antibiotics, it can cause serious illnesses for which there are few effective treatment choices. Due to its ubiquitous presence in the environment, E. coli has traditionally been recognised as the main vehicle for the transmission of various antibiotic resistance genes and vectors.Extended-spectrum beta-lactamases (ESBLs), which can make the bacteria resistant to some frequently used antimicrobial treatments, are tiny proteins (enzymes) that some strains of E. coli have been shown to manufacture under favourable conditions. A major issue around the world is the emergence of different E. coli strains that produce ESBL in foods that come from animals that are raised for food. The ESBL enzymes TEM, SHV, and CTX-M from Group A have been widely reported to be generated by E. coli and have been shown to hydrolyze ampicillin, carbenicillin, oxacillin, and an extended spectrum of cephalosporins, including ceftazidime and cefotaxime.
Extended beta spectrum lactamase producing E. coli
The occurrence of antimicrobial resistance and the presence of corresponding resistance genes in the environment is an ancient phenomenon. This is largely due to the fact that many of today’s antimicrobial agents are derived from natural precursors produced by soil bacteria such as Streptomyces. These natural compounds likely evolved to mediate microbial competition for

Imperfect assimilation and consequent defective nutrition, it goes further and produces neurasthenic states in consequence, and increased susceptibility to nervous stimuli and exaggerated reflexes. Diseases of bones, caries and necrosis. In TB it must be used with care, for here it may cause the absorption of scar tissue, liberate the disease, walled in, to new activities. It is a deep acting remedy and slow in action. It is meant for scrofulous children with large head, open fontanels and sutures, distended abdomen, slow in walking. Ill effects of vaccination. It is related to all fistulous openings. Ripens abscesses since it promotes suppuration.

Silica patient is cold, chilly, hugs the fire, wants plenty warm clothing, and hates drafts, hands and feet cold, worse in winter. Lack of vital heat. Prostration of mind and body. Great sensitiveness to taking cold. Intolerance of alcoholic stimulants/Ailments attended with pus formation.

In inflammation with pus formation, which has become chronic, silicea is of great help. It is equally useful in grandular or bony ulceration. Think of silicea in whitlow, unhealthy skin, smelly feet, mammary abscess, mammary cancer, TB of the skin, the bone or of the abdomen,

1.1. Sericulture

Refer to rearing of silkworms for the production of raw silk.

Major activities are mulberry cultivation, silkworms spin silk cocoons and reeling the cocoons for unwinding the silk filament for value added benefits.

Bombyx mori is the most widely used. Silk-fiber is a protein produced from the silk-glands (modified labial glands) of silkworms.

A silo is an airtight to semi-airtight structure designed for the purpose of preservation and storage of high moisture feeds as silage.
 
Role of the silo in silage making

Silo has only three functions in the ensiling process:
    1.    To provide a solid surface to permit compaction of the mass to eliminate air;
    2.    To protect the ensiled materials from air and water during the storage period;
    3.    Where desired, to aid in the removal of silage by providing a base for unloading equipment.

The principle of silver nitrate staining of proteins is based on the reduction of silver ions to its metallic form. (Silver ions in alkaline conditions bind with proteins through å-amino group of lysine and sulphur groups of cysteine and methionine residues). When the complexed silver ions are reduced in the presence of formaldehyde, they become metallic silver, which is seen on the gel as bands. Staining of proteins with silver nitrate after SDS-PAGE is quicker and ~ 10 times more sensitive than Coomassie brilliant blue staining and that is the reason it is widely used in research laboratories these days.

The procedure is as follows:

Silver Staining for Protein

Silver staining is one of the procedures, in addition to Coomassie Blue, R and G types, and fluorescent dyes that are available for detecting proteins separated by gel electrophoresis. Switzer introduced silver staining in 1979, a technique that today provides a very sensitive tool for protein visualization with a detection level down to the 0.3-10 ng level.

The basic mechanisms underlying silver staining of proteins in gels are relatively well understood. Basically, protein detection depends on the binding of silver ions to the amino acid side chains, primary the sulfhydril and carboxiyl groups of proteins, followed by reduction to free metallic silver. The protein bands are visualized as spots where the reduction occurs and, as a result, the image of protein distribution within the gel is based on the difference in oxidation-reduction potential between the gel’s area occupied by proteins and the free adjacent sites. A number of alteration in the silver staining procedure can shift the oxidation-reduction equilibrium in a way that gel- separated proteins will be visualized either as positively or negatively stained bands. Silver staining protocols can be divided into two general categories: 1) silver amine or alkaline methods, and 2) silver nitrate or acidic methods. In general, the detection levels’ using the various procedures is determined by how quickly the protein bands develop in relationship to the background (e.g. signal-to-noise ratio). The silver amine or alkaline methods usually have lower background and as a result are most sensitive but require longer procedures. Acidic protocols, on the other hand, are faster but slightly less sensitive. Clearly, each protocol has different advantages regarding timing, sensitivity, cost, and compatibility with other analytical methods, especially mass-spectrometry (MS) a tool that is being used in combination with gel electrophoresis or chromatographic methods for rapid protein identification. Until recently most of the silver staining protocols used either glutaraldehyde- of formaldehyde-based sensitizes in the fixing and sensitization step, thus, introducing the chemical modifications into proteins. The utilization of those chemicals causes the cross-linking of two lysine residues within protein chain, thus, strongly affecting two most crucial steps for the subsequent MS analysis: hampering the trypsin digestion and highly reduces the protein extraction from the gel .

Silviculture is the branch of forestry dealing with the establishment, development, care and reproduction of trees. Forestry is anything done in the forest and silviculture is anything done to forests. ‘Silva’ is the Latin word for forest and ‘culture’ for cultivation. Silviculture is narrower than forestry, being concerned only with forest plants, but is often used synonymously with forestry.

The Society of American Foresters defines silviculture as theory and practice of establishment, composition, constitution and growth of forests. Thus, silviculture refers to art and science of raising forest crops, methods of raising tree crops, their growth and aftercare up to the time of final harvesting. In simple words, silviculture is the growing and tending stands of trees. Silviculture is also used to manage forests for wildlife, water, recreation, aesthetics, or any combination of these or other forest uses.