eChapters

Youth in India had been at the forefront of the nation’s struggle for freedom and development. There are over one billion youth in the world today. Eighty five per cent of these youth live in the developing world and around 50 per cent of the population with in developing countries live in rural areas. According to 2001 census, more than 40 per cent of the total population is between the age group of 15 to 35 which is considered as “youth” by the Government of India. Out of which, 73 per cent resides in the rural areas (Anonymous 2001). Unfortunately rural youth of today are counted among the most disadvantaged groups of society. Because of rapid population growth, widespread unemployment inadequate health, poor education, migration, lower family income and lacking adequate opportunity at farm level had led the rural youth in continued stress of self esteem and self confidence. Youth empowerment is an attitudinal, structural and cultural process whereby young people gain the ability, authority and agency to make decisions and implement change in their own lives and the lives of other people. Youth are the present and the future of humanity.

India produce a wide range of tropical, subtropical and temperate horticultural crops at different places by virtue of its varietals agro- climate conditions. The total annual production of fruits and vegetables is around 135.5 million tones, fruits being 48 million tones and vegetables around 87.5 million tones. India is the second largest producer of fruits and vegetable in the world. They are major sources of important valuable vitamins and minerals. Being highly perishable, fruits and vegetables can be preserved and processed either at farm level or at cottage scale/home scale during market glut season or extra seasonal produce from kitchen garden for later use. Farmers and entrepreneurs can set up their own processing units by adopting simple, low cost innovative processing technologies which will save post harvest losses of these perishable crops and also give good returns to grower and better earning to processor/ entrepreneurs.

ABSTRACT

The tissue culture derived black pepper plants of variety Panniyur 4 were compared with conventional propagules for its vegetative growth and field performance. The in vitro derived plants recorded better rooting, survival rate and shoot growth than the conventional propagules. More than 90% of the TC derived plants recovered after primary hardening and 99% recovered after secondary hardening, while it was only 70% in conventional propagules at both the hardening stages. The secondary roots developed in TC derived plants were observed to be far more superior in number and length. The initial growth rate in terms of plant height, number and size of leaves was much better in TC derived plants. The TC derived plants developed numerous bearing shoots - laterals - in the first year of growth and started bearing in the first year itself. The performance was superior to conventional propagules in which production of laterals and bearing begins much later. Thus the performance of TC derived plants was rated as superior to conventional propagules.

India is a varitable emporium of herbs. The medicinal use of plants was known to the inhabitants of India from time immemorial. As per Indian Materia Medica, a large number of vegetables, different parts and products of flora and fauna came into use for the treatment of diseases by ancient Hindus. Generally the practitioners, of various Indian system of medicines used to utilise the flora and fauna found in their environment and accept those which were found useful after trial.Ayurved comes from Atharvaveda. There are hundred fourteen hymns which describe some diseases and medicinal practices. This was the era around 2000 B.C. The books written on Ayurved were rewritten by ‘Charak’. It is known s ‘Charak Sanhinta’. They were written in eight volumes having hundred twenty chapters in Sanskrit Sushrut wrote in similar way under the name of ‘Sushrut Sanhita’ having ten thousand verses in six-volumes under one hundred eighty six chapters. He described ‘Surgery’. Kashyap wrote on ‘Paediatrics’ and Vatsayana wrote on ‘Sexology’. Hence, very rich and old heritage of Ayurved is prescribed and documented in Hindu Vedas. This was the age when art of ‘Pharmacy’ was introduced. There after came the ‘Creative age’, when the active principles of medicinal drugs were adopted. According to Charak, in the Chapter ‘Kalpasophan’, the drugs differ with respect to land, season, source, flavour, taste, potency, post digestive effects and specification and also with respect to their body, morbid tendency, constitution, age, vitality, gastric fire, proclivities, homologation and stage of diseases.Accepting those ideas in 1864 with the help of the then king of ‘Jamnagar’ the famous Baidya, Jandu started a small factory (Rasasala) in Jamnagar. Then in 1910 Jandu Pharmaceutical works was established in Bombay to manufacture Ayurvedic medicines in large scale. Thereafter many small factories were started in different parts of India. The vedic period around 1800 B.C. The classic period of the Indian medicine begins about 600 B.C., where the treatments of various types of ailments have been indicated. Due to Industrial evolution and general economic growth in Eastern countries, there was growth of a systematic Eastern Medicine. Gradually people started to develop confidence in allopathic and ayurvedic medicines were neglected.Many allopathic medicines which were established as life saving drugs, were later found to be harmful to humans. Such medicines have been proved to have side effects which cause damage to health. The modern man of scientific C1ptitude again tries to rotate the wheel of history in search of an alternative source/truth from natural resources. With this view many attempts have been made in India and abroad to unravel the truth, described in Vedas with regards to medicinal effects of flora and fauna. Although the Allopathic medicines are found to dominate over the Ayurvedic medicines, many remote areas of tribals are still continuing the traditional practice of using medicine from their natural surroundings.

The venous blood from the head and neck is carried by two jugular veins (Figure 2-11 B), the internal and external jugular veins on each side. Both of these extend from the base of the head to the thoracic inlet and are located on the ventrolateral part of the neck. The internal jugular vein is smaller while the external jugular is larger one.

The internal jugular vein is formed by the union of the thyroid, laryngeal and occipital veins beneath the wing of the atlas between the mandibular salivary gland and the rectus capitis ventralis major muscle. It passes down the neck in company with the common carotid artery, vagus and sympathetic nerves enclosed within a carotid sheath, receives in its course the muscular venous branches, tracheal vein and the oesophageal vein and terminates by joining the external jugular vein just in front of the thoracic inlet.

The external jugular vein is the larger of the two jugular veins and is formed by the union of the superficial temporal and the maxillary veins just behind the caudal border of the mandible and on the deep face of the parotid salivary gland below the temporomandibular joint. It becomes superficial by emerging through the parotid gland, passes down the neck subcutaneously in the jugular furrow, receives the internal jugular vein forming a common jugular vein in front of the thoracic inlet. It receives along its course, the external maxillary vein, parotid veins, auricular veins, small veins from the muscles of the cervical region and the cephalic vein at its termination. While in the jugular groove it is bounded dorsally by the brachiocephalicus muscle, ventrally by sternocephalicus and medially by the sternomastoid part of the sternocephalicus muscle. The vein is covered by skin and fascia and is separated from common carotid artery, vagosympathetic trunk, recurrent laryngeal nerve and internal jugular vein by the upper border of the sternomastoid muscle.

The right and left common jugular veins unite and form the jugular confluence which joins the cranial vena cava ventral to the trachea slightly to the right of the median plane at the level of the cranial border of the first rib.

What is dermal fibroblast?

Skin fibroblasts are a type of cell found in the dermis, the middle layer of the skin. They are the most common cells in connective tissue and play a crucial role in maintaining the structural integrity of the skin. Fibroblasts are responsible for synthesizing and secreting extracellular matrix proteins such as collagen, elastin, and fibronectin, which provide support and elasticity to the skin. Additionally, fibroblasts play a role in wound healing by proliferating and migrating to the site of injury, where they aid in tissue repair and scar formation.

Aim: To prepare the primary cell culture of fibroblasts from canine skin.

Tools and instrument required for the procedure

1. Tissue collection box with ice cubes - To prevent contamination and maintain the stable temperature

2. Surgical Gloves - To prevent contamination

3. Tissue collection tube with 1X PBS - To collect sample

4. Laminar flow hood - To work in a sterile environment and prevent contamination

5. Cell culture dish - To digest the skin

6. Sterile 1000 μL and 200 μL pipette and tips-For accurate measurement and transfer of cell suspension and culture media.

7. Forceps - Fine-tipped forceps for handling and manipulating tissue.

8. Scalpel and blade holder - Scalpel for the dissection of skin tissue.

9. Surgical scissors - Chopping of the skin tissue

10. Falcon 70 μm cell strainer - To filter out undigested tissue debris and obtain a homogeneous cell suspension.

11. 15 ml and 50 ml Centrifuge tubes - To collect and centrifuge the digested cell suspension.

Objectives
• Understand the stages of the writing process.
• Visualize the flow of writing stages using a graph.
• Apply effective strategies for each stage of writing.
Introduction
The writing process is a structured approach that helps writers create clear and coherent texts. It generally consists of five main stages: prewriting, drafting, revising, editing, and publishing. The stages are interconnected, and writers often cycle back to earlier steps before finalizing their work. The following graph will help visualize how these stages overlap and repeat during the writing process.
Graph of the Writing Process
The graph below represents the cyclical nature of the writing process:

The custard apple is believed to be a native of the West Indies but it was carried in early times to southern Mexico through Central America. The custard apple, is generally rated as “ugly duckling” species among the prominent members of the genus Annona. It is sometimes erroneously termed “sugar apple” or “sweetsop”. The custard-apple is also called bullock’s heart or bull’s heart. The name “Custard Apple” is due to its custard like taste. The fruit has a peculiar aroma which makes a sweet drink and can be used as a milk substitute. Custard apple fruit with a sugary, grainy taste is rich in calories. The fruits are packed with vitamins (A, B and C) and minerals (iron, calcium, magnesium and potassium). The fruit flesh can be scooped from the skin and eaten as is or served with light cream and a sprinkling of sugar. Often it is pressed through a sieve and added to milk shakes, custards or ice cream.

I. FILL UPS

1. _____ is a crossing between two genetically dissimilar parents.
2. Detasseling is done in _____.
3. _____ is an example for chemical hybridizing agent.
4. _____ is called as pollen parent.
5. _____ is the fertile counter part of A line.
6. DUS stand for _____.

ABSTRACT

The in vitro culture studies in crop plants show that there is a differential response of various explants to different concentrations of auxins and cytokinins. This indicate that hormonal requirement is specific to the genotype and developmental stage of the explant. Therefore standardisation of media and explants were done for in vitro callus culture and plant regeneration in L. esculentum and L. pimpinellifolium at the Department of Olericulture, College of Horticulture during 2000-2002.

The source materials selected for the study were L. esculentum var. Sakthi and L. pimpinellifolium. The explants were leaf segments (0.5 cm²) from fully opened leaves, nodal segments (0.5 cm) and internodal segments (1.0 cm). The media consisted of the basal MS and ½ MS media along with different auxins and cytokinins either singly or in combination. The auxins used were NAA (0, 1, 2 and 3 mg l^-1 and 2,4-D (0.5, 1, 1.5 and 2 mg l^-1) and the cytokinins used were BA and kinetin (1, 2, 3, 4 and 5 mg l^-1).

This practical assignment is designed for the students to give first-hand information on components, working procedure and handling of public address system (PAS) and liquid crystal display (LCD) projector.

PURPOSE

 To acquaint students with how to operate and handle the public address system.

 To acquaint the students handle LCD projector.

Herbicide Mixtures

A mixture of two or more herbicides could be more effective for controlling different weed species. In horticultural and plantation crops, weed free situation is needed during the entire year over several years. In such situations, herbicides are to be rotated to take care of all the species of weeds. A mixture of herbicides, due to chemical reaction, may enhance or reduce the efûciency of one or more compounds. Hence, knowledge of herbicides interactions is essential for efûcient use of herbicides in weed control.

Morphological characteristics

Abroma is a shrub or small tree that grows to a height of around 3-5 metres. The plant has horizontal and velvety branches that are 10-30 cm long, 6-18 cm wide, oblong or lanceolate, more or less cordate, delicately acuminate, membranous, whole, and three to five lobed with a petiole that is 1-12 cm long. The dorsal surface of the leaves is glabrous, while the ventral surface is hairy.

Floral characteristics

The plant has beautiful and intricate scarlet like flowers (5 cm in diameter) bearing purple colour. The inflorescence is a cyme. The sepals are persistent and lanceolate, measuring about 2.5 cm long. The petals are imbricate and will shortly fall off. Stamens are situated on a short staminal tube, and there are five staminodes. Each cell in the ovary is five lobed and pyramidal, with numerous ovules. The apex, the capsules are obpyramidal, membranous, finely pubescent, and truncate at the apex. Behind each carpel is a triangular wing. December and January are the fowering and fruiting months.

Site of incision: Make a straight dorso-ventral incision through skin from the summit of dorsal vertebral spine to the cranioventral aspect of shoulder joint. Extend it up to the cranial margin of shoulder joint, then caudo-ventrally up to caudal most part of elbow joint. Make another ventrodorsal straight incision from caudal most point of elbow joint to the summit of vertebral spine then reflect the skin flap from ventral to dorsal up to mid dorsal line of the body. Remove the facia to expose the various muscles of this region.

What is subculture?

In cell culture, a process called subculturing, or passaging, keeps cells alive and growing. It involves taking cells from an existing container, removing the used medium, and placing them in a new container with fresh nutrients. This allows the cells to continue multiplying and creates more cells for research.

Adherent subculture protocol Cells nearing 80% coverage need splitting!

1. Warm things up: Get your media, detachment solution, and incubator ready at 98.6°F (37°C).

2. Safety first! Disinfect your work area and supplies inside a biosafety cabinet.

3. Gently rinse: Remove the old media and wash the cells with a salt solution at room temperature. Be careful not to dislodge the cells.

4. Detach the cells: Add a pre-warmed enzyme solution to detach the cells from the flask surface. Keep an eye on them to make sure it works (usually 2 minutes). Note: Not all cells need this enzyme, and it can be harmful to some.

5. Stop the enzyme: Once all the cells are detached, add a culture medium with serum to neutralize the enzyme.

6. Collect the cells: Transfer the cell suspension to a tube, rinse the flask with fresh medium to get all the cells, and add it to the same tube.

7. Spin it down: Centrifuge the cell suspension for 5 minutes at room temperature (1000 rpm).

8. Get ready for counting: Remove the liquid, loosen the cell clump, and resuspend the cells in a fresh medium at a good volume for counting.

9. Count your cells: Use a hemocytometer to count the cells and determine their health.

Introduction
In order to study the cultural, morphological and physiological characteristics of an individual species, a pure culture of the microorganisms is required. Several different methods of separating the microorganisms from a mixed culture are available. The two most frequently used methods involve making a pour plate and/or streak plate. Isolated colonies are obtained from individual organisms after incubation.
Streak Plate Method
Plating is generally applied to the inoculation of a medium in petridish. Streak plate method is best for the separation of organisms from a mixed culture. In this method, agar medium is allowed to solidify as a thin layer in a petri dish and culture is streaked on the surface of agar medium and allowed to incubate to get isolated colonies.
Materials
1. Sterile petri plate
2. Wire loop
3. Nutrient agar medium
4. Bunsen burner
5. Mixed culture of E.coli and Micrococcus leutus

This chapter introduces ergonomics as the science and practice of designing tools, systems and environments around human capabilities and limitations. It explains the meaning, significance and principal objectives of ergonomics, including safety, comfort, efficiency and human well-being. The historical development of the discipline is traced from early toolmaking and industrialization to contemporary human-centred design. Anthropometry is introduced as an essential basis for matching furniture, equipment and workplaces to different users. Examples from domestic life, education and professional settings demonstrate the practical relevance of ergonomic thinking. The chapter establishes human-centred design as the foundation for developing functional, safe and accessible solutions.

Year                    Milestones
1951    :    First amino acid was sequenced by F. Sanger and H. Tuppy.

1953    :    J.D. Watson and F.H.C. Crick proposed that DNA is a double helix.

1958    :    Discovery of DNA polymerase by Kornberg’s group and DNA replication studies began.

1959    :    Isolation of RNA polymerase.

Principle

Orchid seeds are produced in large numbers but are exceedingly small, hence are also called “dust seeds” may be as small as 0.01 by 0.05 mm in dimension and weigh as little as 10-30 ug. Seedling development is generally a slow process in orchids, with the seedlings passing through an initial stage, termed the protocorm stage, prior to the production of distinct vegetative organs (leaves, roots, etc). The protocorms of terrestrial orchids are subterranean and entirely heterotrophic, with all of the nutritional needs of the orchid being supplied by the mycorrhizae (the symbiotic fungus). The length of the protocorm stage is variable, however, in many cases, months, or even years, elapse before leaves and roots are produced.

The combination of a small seed size and fungal symbiont requirement largely precludes the propagation via conventional seed propagation protocols. Accordingly, most of the early research on the development of orchid seed germination protocols focused on in vitro germination with the seeds and fungal symbiont being sown on the same petri plate. Successful protocols were developed for several species; however, the process was complicated by the diversity of fungal symbionts required (across orchid taxa) and the difficulty in isolating and culturing these fungi.

In the 1920’s the process of orchid seed propagation was revolutionized by Lewis Knudson with the demonstration that nutrient media that could be used as a substitute for fungal infection. The isolation and culture of multiple fungal strains was no longer required, and improvements in germination frequency, germination speed, and seedling vigor were generally observed. To date, successful asymbiotic germination techniques, termed ‘flasking’, have been developed for many epiphytic orchids, with several species being saved from extinction via the application of these techniques.