eChapters

ABSTRACT

Attempts were made to induce variability by irradiation of axillary buds of rose cv. Folklore followed by in vitro culture. Axillary bud explants collected at different growth stages were subjected to gamma irradiation at different doses (20, 30 and 40 Gray). The in vitro induced micro shoots were sub cultured on a rooting medium (M.S. basal medium supplemented with IAA 1.00 mg/l + NAA 1.00 mg/l + activated charcoal 500 mg/l). The rooting percentage was the lowest in explants irradiated with 40 Gy. Rooting efficiency was severely affected when buds were collected at vegetative phase. Significant interaction between bud stages and gamma rays was noticed for days to root initiation and number of roots per culture. Gamma irradiation at 30 and 40 Gy significantly delayed root initiation at all stages of buds exposed. Significant reduction in number of roots/ culture, and root length was observed at higher doses of gamma exposure. The reduced rooting efficiency may be due to inhibition of cell division on account of chromosome damage.

Fusarium wilt of carnation caused by Fusarium oxysporum f.spp dianthi causes severe yield losses under protected cultivation. For the management of Fusarium wilt, 27 isolates of Bacillus strains were isolated from the rhizosphere of different crops including carnation. Among various species of Bacillus, Bacillus amyloliquefaciens (BSC7) was identified as a most virulent antagonistic bacterial isolate. Studies on antagonism under Environmentally Controlled Scanning Electron Microscopy (ESEM), depicted the presence of rod shaped bacterial cells with biofilm formation at 44847x. Detection for the presence of antibiotic biosynthetic genes reflected the presence of surfactin, iturin, bacillomycin and fengycin genes. The 24 isolates of Bacillus spp. showed positive reaction for surfactin gene. Nineteen isolates were amplified for Iturin A gene except BS5, BS9, BSC1, BSC2, BSC11, BSD1 and BSD2. Strains of B. subtilis BS1, BS2, BS5, BS6 and BS8 expressed Bacillomycin D gene. The genomic DNA of strains BS1, BS2, BS4, BS6, BS7 BS8, BS11 and BS14 showed positive for Fengycin D gene. The isolate of B. subtilis BS2 showed positive reaction for four genes namely surfactin, iturin, bacillomycin and fengycin.

In vitro screening of Bacillus, B. amyloliquefaciens (BSC7), P. aeruginosa (P1) and T. viride (TV1) showed highest inhibition against F.oxysporum f.sp.dianthi to an extent of 55.95, 55.29 and 81.61 per cent over the control respectively. The antifungal activity of antagonistic B. amyloliquefaciens BSC7 and T. viride TV1 against F.oxysporum f.sp. dianthi was confirmed under ESEM by observing disintegration, lysis and structural abnormalities of the hyphae of the pathogen at the zone of interaction.

Bulk population breeding developed by the Swede H. Nilsson Ehle in 1908 and later on supported and practiced by H. V. Harlan in barley breeding in 1940s is a breeding strategy in crop improvement in which natural selection is allowed to play its full role where the F2 generation is planted in a plot large enough to accommodate several hundreds of plants. Planting density and cultural practices are usually the normal ones followed for commercial crop planting. At maturity, the plot is harvested in bulk and the seeds are used to plant a similar plot in the next season. This process is repeated till at least F5 where the plants are space planted to allow individual plant evaluation for effective selection. During period of bulk propagation natural selection plays a key role in shifting gene frequencies in the bulk population. This role is of crucial importance as bulk handling is continued over a number of generations. Bulk population handling can be aided by artificial selection at any time during the period of bulk propagation. Aggressive and highly competitive but undesirable plants may be physically rogued out of the population to avoid increasing the frequency of undesirable genes. For example, determinate bush types of beans do not compete well against indeterminate, vine types. And if the breeder is interested only in determinate types, artificial selection against the indeterminate types is certainly called for. There is also good reason to apply artificial selection against seed colour, pubescence, awns or other characters that are undesirable in the variety to be developed but may be more or less neutral in survival. Bulk population handling often provides opportunities to make use of artificial aids to selection. For example, bulk harvesting when only part of plants are mature is an effective and inexpensive way to select for earliness. If particular sizes of seeds are required, screens or mechanical devices provide a rapid way of eliminating inferior types. Naturally occurring or artificially induced disease or insect infestation, of course, provide opportunities to rogue susceptible individuals from the bulk plot. In F6, the plants selected in F5 are progeny rowed and superior progenies are selected from where again few plants can be selected and seed bulked within a selected row. Depending upon seed requirement for yield trial in the following years, even the seed from selected entire row can be bulked. The follow up yield evaluation trials are as given in case of pedigree selection. This method is operationally best suited for seed crops such as small grains, beans and soybean and is almost totally unsuited for fruit crops and for most vegetable crops.

1.1. Plant breeding

An art and science, deals with ways and means to change the genetic architecture of plants so as to attain a particular objective.

For ensuring food security, it is important to breed and develop new varieties that are high-yielding, pests diseases and drought resistant or regionally adapted to different environments and growing conditions.

There is a very impressive and extensive number of terms and definitions used in plant pathology, many of which you will not come in contact with. With interest, study and practice, terms and names will come to light and become familiar to you. When you start out, don’t worry too much about the terms; but also don’t be afraid of them. The more exposure you have to the subject the more comfortable you will be when dealing with your peers and the public. With these you are going to be exposed to some terms and definitions that will be a good ice breaker and prepares you for the information to follow

Morphological characteristics

Caesalpinia sappan is a thorny, small to medium-sized plant with prominent spines on the stem and leaf rachis that grows up to 10 metres tall. Branchlets are lenticellate, dull, and usually have paired, recurved stipular prickles. With 9–14 pairs of pinnae, the leaves are big, hairy, or glabrate.

Floral characteristics

The inflorescence is a supra-axillary and terminal raceme with golden yellow flowers that creates a huge panicle. The fruit is an elliptical pod that ranges in colour from brown to black. The peak flowering and fruiting season is from July to September, with November being a rare exception.

Distribution

The crop is native to the Indo-Malayan region. The species can be found throughout the Indian peninsula. It is also produced as a plantation crop in all of the Southern states, as well as in Odisha and Bengal to the north.

11.1. Origin and distribution

• Native of Atlantic Coast from Mexico to Brazil.

• Vanilla comes from orchids of the genus vanilla.

• Originally came from Mesoamerica, including parts of modern day Mexico and Guatemala.

• Major vanilla producing countries are Java, Mauritius Tahiti, Seychelles, Madagascar, Indonesia, Mexico, Cameroon and Reunion.

• Madagascar and Indonesia produce two thirds of the world’s supply of vanilla.

• In India, vanilla cultivation is gaining importance in Kerala, Karnataka and Tamil Nadu since early 1990s.

11.2. Economic importance

• Second most expensive spice after saffron

Site of incision-Make a rostro-caudal incision along the ventral border of horizontal ramus of mandible then extend cranio-dorsally along caudal margin of nostrils up to middorsal line of face. From the caudal end of 1st incision make another incision dorsally up to base of the ear then passes cranially along the ventral aspect of eye up to mid dorsal line of face. Reflect the skin carefully from ventral to dorsal, carefully remove the muscle zygomaticus, malaris and nasolabialis as these are firmly attached to skin.

Principle

Isolating the virus in a laboratory is the gold standard for diagnosing many viral infections. Virus isolation is a highly specialised technique used to diagnose viral infections and produce a stock of viruses for research purposes. The isolation of the virus relies on the suitability of the sample, the ability of the cells to allow entry, the successful replication of the virus, and the release of viral particles from the cells. The choice of cell lines is dependent upon the suspected viral disease and the availability of such cell lines in the laboratory.

Materials required

Equipment

a) Biosafety cabinet level 2 (B2)

b) Carbon Dioxide (CO2 ) Incubator (set to 37°C with a 5% CO2 in air atmosphere)

c) Refrigerated centrifuge

d) Refrigerator

e) Inverted Microscope

f) Water bath

g) -86°C/-80°C deep freezer

h) Autoclave

i) Hot air oven

j) Filtration Assembly

1. Introduction

Libraries play an important role in developing reading habit among students. The Academic library specially means to support the teaching learning process and is focus to work on curriculum based education system. Inculcating reading habit among students in academic Library is a challenge. Our College SMRK BK AK Mahila Mahavidyalaya, Nashik is dedicated towards the Women’s Education has accepted this challenge and established Book Club to inculcate reading habit among students. Reading Drive is one of the unique practices run in our college from 2014. Every year more than 200 students enrolled under the Book Club and read the books other than academics. Under this club, we take various activities such as Book Review Competition, Book talk, Books Exhibitions on Various Occasions, Digital Exhibition of Book Reviews submitted by students, New Arrivals Desk, Ask a Librarian and execute this Reading Drive.

As the temperature rises during the summer months, it's crucial for pet owners to be vigilant about the well-being of their furry companions, especially dogs. As dogs are very much playful and active in nature, constant monitoring or observation is required to assess onset of heat stress as its onset is very quick and require immediate attention. Hence, knowing the sign and symptoms of heat stress will save the pets from ensuing negative impact on the health. Proper care and management are essential to ensure your pet stays healthy and happy during this adverse climatic condition.

Dogs release their body temperature through panting and by sweating through the nose and paw pads. If they can’t dissipate heat sufficiently their internal body temperature raises leading to hyperthermia (excess body temperature than normal, 101.0 to 102.50F).

Signs and symptoms of heat stress

The signs and symptoms vary as per the degree of stress incurred. At the initial stage of stress they show – increased thirst and panting.

The style of technical communication focuses on delivering information clearly, concisely, and accurately, often in a professional or academic
context. Technical communication is typically used in fields such as science, engineering, technology, and medicine to convey complex data or instructions to a specific audience.
Characteristics of Technical Communication Style
• Clarity: The message should be easy to understand without ambiguity.
• Conciseness: Information is conveyed using as few words as necessary.
• Accuracy: The content must be precise and free from errors, especially when explaining technical data.
• Objectivity: The style is factual and free from personal bias or opinions.
• Consistency: Terminology, units of measurement, and formatting should remain consistent throughout the document.
Types of Technical Communication
• Instructions/Manuals: Step-by-step guides for completing a task or operating machinery.
• Reports: Detailed documentation of findings or research.
• Proposals: Formal suggestions for a project or plan.
• Emails/Business Letters: Professional correspondence.
• Presentations: Visual and oral methods of conveying technical information.

India’s agrarian culture and economy is undergoing drastic changes. In this progressive path of agricultural resurrection, a comprehensive multidimensional understanding of agricultural development is of great importance. In the present context of Kerala, where the local administrative bodies are vested with more powers to plan and execute need based agricultural development activities, a clear understanding of the concept of agricultural development among the major stakeholders shall serve as a firm base for development.

I. FILL UPS

1. _____ is basically a critical assessment of effective seed requirement.
2. _____ is the ratio of seed yield per seed generation.
3. The quantity of quality seed that have replaced the actual seed requirement of the location is _____.
4. SRR for hybrid seeds is _____
5. Seed multiplication ratio for groundnut _____

Taxonomic classification of avian species is beyond the scope of this booklet. However, since the preliminary knowledge of bird species for students of avian medicine class is of interest, the following description of most common pet and wild birds are being attempted for their benefit.

GALLINEFORMES: This order includes domestic chicken, turkey, pheasants, peafowl, grouse, quail, guinea fowl, guans and curassows.

COLUMBIFORMES: Includes pigeons and doves.

PSITTACIFORMES: Includes parrots, which have strongly hooked maxilla with a flexible attachment to the skull. They also have dexterous zygodactyl feet (two digits point forward and two backwards). The order includes.

This practical assignment deals with identification of emerging enterprises in agricultural sector and helps students in identifying the emerging enterprises suitable for the study area on the basis of the situational analysis.

PURPOSE

 To provide a comprehensive idea about different enterprises.

 To acquaint the students with different factors which are essential for identifying emerging enterprises in agricultural sector.

Classical biological control is one of the important tools for the management of invasive species. This involves the introduction and release of natural enemies, usually from the invaders’ area of origin. During the past decade, increasing concerns have been raised about the potential negative impact of introduced biological control agents on non-target organisms. This has highlighted the need to examine and guide the process of importation and release of natural enemies. The third international standard for phytosanitary measures, ISPM 3: Code of conduct for the import and release of exotic biological control agents, is specifically concerned with this process. ISPM 3 was endorsed by members of the Food and Agriculture Organization of the United Nations in 1995.

There is a growing demand and need for biological control across the globe. This has been fuelled not only by the increasing spate of invasive species but also by the need to develop integrated pest management approaches as well as the increased interest in organic production. Classical biological control of arthropod pests and weeds has been carried out successfully for more than a century (Greathead and Greathead, 1992; Julien and Griffiths, 1998). However, over the years, practitioners have become increasingly aware that introduced biological control agents may have undesirable side-effects (Thomas and Willis, 1998; Howarth, 2000; Follet and Duan, 2000; Lynch and Thomas, 2000; Wajnberg, Scott and Quimby, 2000; Henneman and Memmott, 2001). Initially, this concern was limited to the possible impact of these introduced agents on economically important plants and insects (notably, honey bees, silk moths and weed biological control agents). More recently, increased environmental awareness has drawn attention to the potential danger to all indigenous fauna and flora, particularly rare and endangered species.

To address these concerns, a code of conduct for the import and release of exotic biological control agents was developed with a view to fostering safe importation and release of biological control agents (Greathead, 1997). It was developed over a period of six years and was finally endorsed by FAO member countries at the end of 1995. The code of conduct, the third in a series of international standards for phytosanitary measures developed by the International Plant Protection Convention, was formally published in 1996 as ISPM 3: Code of conduct for the import and release of exotic biological control agents and is available, with other ISPMs

5.1 Ringworm (in Cattle) causing fungi

Trichophyton verrucosum

Ringworm is one of the commonest skin diseases in cattle. Ringworm is a transmissible skin infectious disease caused most often by Trichophyton verrucosum, a spore forming fungi. The spores can remain alive for years in a dry environment. Although unsightly, fungal infections cause little permanent damage or economic loss. Direct contact with infected animals is the most common method of spreading the infection.

The symptoms of Ringworm include Lesions with greywhite areas with an ash like surface usually circular in outline and slightly raised that appears on skin. Size of lesions vary and can become very extensive. In calves the infection is most commonly found around eyes, on ears and on back. In adult cattle infection on chest and legs are more common (Fig. 183)