eChapters

Introduction
The identification of crop varieties is crucial in improving agricultural productivity, ensuring quality control, and supporting breeding programs. The correct identification helps improve food security, conserve biodiversity, and manage pests and diseases. Traditionally, farmers were relying on visual traits and phenotypic characteristics, but these methods had their own limitations. Modern molecular techniques, such as DNA fingerprinting and genotyping, have revolutionized variety identification, rendering it both more accurate and efficient. This has allowed for early identification of varieties, assisting in the selection of desired traits and enhancement of crop resilience, thus contributing to more sustainable and productive agricultural practices (Azizi et al., 2021; Khan et al., 2024).
Importance of Crop Variety Identification in Agriculture
Crop variety identification remains one of the most important components in agriculture since it can improve crop production, enhance food security, and improve adaptability of the crops to diverse environmental conditions (Hasan et al., 2021). The major aspects related to correct identification of crop varieties include:
• Quality control and standardization: It is often to measure the quality of crops that could meet market demand, for optimum consumption, and for export.
• Biodiversity conservation: This helps to conserve native crop varieties and regards the preservation of genetic diversity that is key for breeding and in future agricultural resilience.
• Breeding program: It allows the identification of a variety for selecting traits such as disease resistance, drought tolerance, and yield potential for improving agricultural productivity through its effectiveness.
• Pest and disease management: It contributes to pest and disease management too since different crop varieties may express varying levels of resistance which also reflects in pest control strategy.
• Market preferences and economic value: This helps the farmers to supply some specific markets depending on the market demand of certain varieties so as to gain a profit (Hussain & Nisar, 2020; Sharma & Singh, 2021; Tazeb et al., 2018; Dheer et al., 2020; Jamali et al., 2019).

Vegetables belonging to the family Brassicaceae (Syn: Cruciferae) are known as Cole crops.  These are the most widely grown vegetables in temperate and sub-tropical zones.  The word ‘Cole’ comes from abbreviation of word ‘caulis’ meaning stem.  Cole crops are a group of highly differentiated plants originated from a wild plant Brassica oleracea var. oleracea (=B. sylvestris L.) commonly known as “Wild cliff cabbage” or “colewort”, through mutation, human selection or adaptation. Cole crops include cabbage, cauliflower, knol khol, Brussels sprouts, Chinese cabbage, sprouting broccoli, kale etc. of which cabbage and cauliflower are the most popular and widely grown.

Since floricultural and ornamental crops are grown for aesthetic purpose and are non-edible there is likely to be less concern in bio safety issues compared to other food crops. Hence there is considerable potential for developing transgenics in ornamental crops. Advances in transgenic technology provide new opportunities for manipulation of the genome. These will have significant impact on expanding and diversifying the gene pool of crop plants, introducing specific genes and shortening the time required for the production of new varieties or hybrids. Molecular breeding is beneficial to increase the production and quality by creating plants with enhanced resistance to diseases, insects or viruses and increased tolerance to environmental stresses like salinity, temperature or drought. Through this technique genes for shelf life, flower colour and architecture may be directly transferred to develop new varieties that are tailor made to customer preferences. Bioluminescent orchids and blue roses are the significant outcomes of the genetic transformation studies in ornamentals (Swarnapiria, 2009).

Coffee is grown at higher elevation across the Western Ghats between 750 and 1200 m amsl. It requires a certain degree of shade for its better performance.  The annual rainfall over the coffee growing tracts varies between 1400 and 2600mm, having uni/bi-model rainfall with prolonged dryspell from November to May. It is more so over North of the Western Ghats. The mean annual maximum temperature varies between 24 and 300C while the minimum between 15 and 180C. The difference between maximum and minimum temperatures (temperature range) varies between 5 and 160C across the coffee tract. The ‘Monsooned Malabar Coffee’ is a specialty coffee produced from the erstwhile ‘Malabar Region’. It undergoes certain changes in physical and biochemical characters when the coffee beans are processed during the summer monsoon (June to September). It is processed at Mangalore, located in Coastal Karnataka. The processing location experiences heavy rainfall of more than 1000 mm in July, followed by 950 mm in June and 600 mm in August. Out of 3264 mm of annual rainfall, 2852 mm is received during the monsoon period. It accounts more than 80 per cent of annual rainfall. The maximum temperature during the coffee monsooning period varies between 28 and 30°C while night temperature revolves between 22 and 24°C. Being located in the tropics along the West Coast, the crop processing environment in terms of surface air temperature is relatively uniform and moderate with a characteristic feature of heavy clouds and continuous rainfall during the monsoon period. These monsoon features are more predominant from the middle of June to August during which the coffee beans are subjected to monsooning. The coffee beans
bulge in the process of monsooning and the volume increases significantly due to absorption of atmospheric moisture. It is directly related to the monsoonal wind during which coffee beans are processed. The bulging was less in all the samples processed during monsoon 2003, which varied from 2.1 to 2.7 ml/100 number, while it ranged between 2.7 and 5.8 ml/100 number in the samples processed during monsoon 2004.

Previous Year Questions

1. Different Classes of Seeds?

2. Various parameters of field & seed standards for breeder seed production?

3. Factors that affect the longevity of seeds during storage in warehouses?

4. Initial Seed Quality?

5. Seed Testing?

Different Classes of Seeds

1. Nucleus Seed: It is handful of seed maintained by concerned breeder for further multiplication. The nucleus seed will have all the characters that the breeder has placed in it and it is of highest genetic purity. The quantity of nucleus seed is in kilograms.

2. Breeder Seed (BS): Breeder seed is a seed or vegetative propagating material directly controlled by the originating or sponsoring plant breeder of the breeding programme or institution and or seed whose production is personally supervised by a qualified plant breeder and which provides the source for the initial and recurring increase of foundation seed. Breeder seed is exempted from certification. However, the breeder seed crop is being inspected at appropriate stages by a Monitoring Team consisting of breeder, representative of NSC and representative of Seed Certification Agency. The breeder seed lots are affixed with Golden Yellow tags. The genetic purity of breeder seed crop should be maintained at 100 per cent.

Introduction
Plant protection is a critical component of modern agriculture, aimed at safeguarding crops from diseases, pests, and weeds that can significantly impact yield and quality. Chapter 10 explores the science of plant protection, focusing on the various disciplines that contribute to maintaining healthy crops and ensuring food security. The chapter begins with an overview of plant pathology, the study of plant diseases caused by pathogens such as fungi, bacteria, viruses, and nematodes. Key principles of plant pathology are discussed, including the nature of plant diseases, their modes of transmission, and the factors that influence disease development. This section also highlights the importance of accurate disease diagnosis, which is essential for implementing effective prevention and control measures. Entomology, the study of insects and their interactions with plants, is another crucial aspect of plant protection covered in this chapter. The principles of entomology are examined, with a focus on the identification and management of insect pests that threaten crops. Various pest management strategies, including biological, chemical, and cultural methods, are explored, emphasizing the need for a holistic approach to pest control. Integrated Pest Management (IPM) is a central theme in plant protection, combining multiple strategies to manage pests in an environmentally sustainable manner. The chapter discusses the advantages of IPM, such as reduced reliance on chemical pesticides and minimized environmental impact, as well as the challenges associated with its implementation. Integrated Disease and Pest Management (IDPM) is also introduced, which integrates plant pathology and entomology principles to develop comprehensive management strategies that address both diseases and pests. The chapter further explores the role of pesticides in plant protection, including their benefits and potential risks. Safety measures for pesticide use are emphasized to protect both human health and the environment. Integrated Weed Management (IWM) is also discussed as an essential component of plant protection, with strategies for managing weeds that compete with crops for resources. The importance of plant protection sciences in food security is highlighted, particularly in the context of pest management, disease control, and weed management. These disciplines contribute to sustainable agriculture by enhancing crop yield and quality while promoting environmental sustainability.

Importance

Ajwain is an important seed spice and is also useful for its medicinal values. It is a traditional potential herb for curing many ailments in human and animals. It is usually used as a digestive aid, relieves abdominal discomfort due to indigestion and antiseptic. The seeds of ajwain is bitter, pungent and contain 2-4.4% brown coloured oil known as ajwain oil. The main component of this oil is Thymol, which is used as gastrointestinal ailment, lack of appetite and bronchial problems. Thymol is also used in toothpaste and perfumery (Joshi, 2000). Seeds are also used for curing abdominal pains, abdominal tumors and piles (Krishnamoorty and Madalgeri, 1999). In southern parts of India dry ajwain seeds are powdered and soaked in milk, which is then filtered and fed to babies as it relieves colic in babies. It can be used as digestive mixture in large animals.

ABSTRACT

Approximately 30 % of fruits and vegetables are getting wasted each year in country like India. Not only that, there is a huge loss of agro based commodity during industrial processing and will be considered as industry generated waste. Utilization of such unexplored nutrients from agro processing sectors for making a sustainable food system will be a healthy approach to bring food security and nutrition to all of us. With this aim the present work addresses the utilization of jackfruit seeds, an agro-waste, to value added products. Jackfruit seed flour has a great potential in new food formulation along with wheat flour. The 15% (w/w) blending of jackfruit seed flour with wheat flour could be used in bread and the substituted bread is protein and fibre enriched. This attempt in developing such bakery items with blended flours could address the scarcity problem of non-wheat producing countries. Present study also looks into the alternate source of starch from spice processing industries. The nutrient starch could be explored for isolation from a nonconventional source from turmeric processing sector. The isolated starch could also be modified to enhance some of its functional properties and may be utilized in preparing value added product with a better acceptability and nutrition.Thus, the isolation of nutrients and value-added products out of fruits and vegetables and spice-processing waste would be a stepping stone to an initiative to zero hunger.

Introduction

Seafood provides about 25% of the global intake of animal protein and fetches a huge economy for developing nations. Many challenges concerning food safety and quality exist due to globalization and liberalization of the global f ish trade. International food safety regulatory bodies such as WTO and CAC through SPS and TBT Agreements work on ensuring food safety and quality. Adoption of risk-based analysis and implementation of the HACCP system are encouraged to maintain high seafood safety standards. International regulatory bodies set maximum residual limits for various environmental pollutants and contaminants to ensure consumer safety. Consumer education programs play important role to enhance awareness and improve transparency in the seafood supply chain. Internationally traded seafood products therefore need to meet the standards prescribed for food safety and quality requirements. In case of non-conformance, they could be detained, rejected and destroyed.

The global demand on seafood quality and safety requirement and the complexities of seafood supply chain make difficulty to ensure authenticity and traceability of seaood products in the market. Most seafood is exported from the country where it is caught while part of it via. a third country. Seafood pass-through complex supply chains involving, capture/culture, transshipment, processing, packaging, wholesale, retailing, and consumer. The global trade of seafood has led to a significant concern associated with seafood fraud.

12.1. Seafood Fraud

Seafood fraud is the practice of providing misleading information to the consumers about their product for financial gain. There are nine types of seafood fraud, which include species substitution, fishery substitution, IUU substitution, species adulteration, chain of custody abuse, catch method fraud, undeclared product extension, modern day slavery, and animal welfare

Maize (Zea mays L.) is one of the most versatile crops having the highest genetic yield potential among cereals and wider adaptability under varied agro-climatic conditions rightly earning it the name of ‘the queen of cereals’. Maize is cultivated in all agricultural areas around the world and is an economically significant crop for all human population. It is grown from 58⁰ L north to 40⁰ L south and extends from the sea level up to an altitude of above 3,800 m; covering regions with rainfall varying from 250 to 10,000 mm (Hallauer and Miranda, 1988). Globally, maize occupies an area of about 184.0 million ha with production of 1065 million tons and productivity of 55.2 q/ha (FAOSTAT, 2014). It is the third most important cereal crop and a major source of energy, protein and other nutrients for human and livestock in the world (Jompuk et al., 2011). India is the second most important maize growing country in Asia, and now ranks as the world’s fifth largest consumer of maize. It is an important cereal crop in India covering an area of 9.42 million ha with a production of 24.35 million tonnes and productivity 25.83 q/ha (Anonymous, 2015). Maize is cultivated in the country throughout the year mostly during rainy or Kharif season (June to September, 80%) followed by winter or Rabi (November to April,18%) and spring (February to May, 2%) for various purposes including grain, fodder, green cobs, sweet corn, baby corn and popcorn.

There is need to find better ways and solutions to mitigate future agricultural challenges toward increasing productivity across the crops and equip them with the desirable genes for resistance to biotic and abiotic stresses and to mitigate the impact of climate change and also to make them nutritionally superior. Here comes “Plant Genomics”—a newly evolved discipline of plant sciences—targeting to decode, characterize, and study the genetic composition, structures, organizations, functions, and interactions/networks of all plant genes in a genome-wide scale. Being evolved from plant molecular genetics, biology, and biotechnology, plant genomics represents the key sub- divisions of structural, functional, comparative, evolutionary, physiological, and genetical genomics. Its development and advances, however, are tightly interconnected with plant science sub-disciplines, such as proteomics, metabolomics, epigenomics, phenomics, metagenomics, transgenomics, breeding-assisted genomics, bioinformatics and system biology as well as modern instrumentation and robotics sciences.

Plant genomics is a recent convergence of many sciences. Simply stated “Genomics” is the new science that deals with the discovery and noting of all the sequences in the entire genome of a particular organism. The genome can be defined as the complete set of genes inside a cell. Genomics, is, therefore, the study of the genetic make-up of organisms. Determining the genomic sequence, however, is only the beginning of genomics. Once this is done, the genomic sequence is used to study the function of the numerous genes (functional genomics), to compare the genes in one organism with those of another (comparative genomics), or to generate the 3-D structure of one or more proteins from each protein family, thus offering clues to their function (structural genomics). In crop agriculture, the main purpose of the application of genomics is to gain a better understanding of the whole genome of plants. Agronomically important genes may be identified and targeted to produce more nutritious and safe food while at the same time preserving the environment. Genomics is an entry point for looking at the other ‘omics’ sciences. The information in the genes of an organism, its genotype, is largely responsible for the final physical makeup of the organism, referred to as the “phenotype”. However, the environment also has some influence on the phenotype. DNA in the genome is only one aspect of the complex mechanism that keeps an organism running – so decoding the DNA is one step towards understanding the process. However, by itself, it does not specify everything that happens within the organism. The basic flow of genetic information in a cell is as follows. The DNA is transcribed or copied into a form known as “RNA”. The complete set of RNA (also known as its transcriptome) is subject to some editing (cutting and pasting) to become messenger-RNA, which carries information to the ribosome, the protein factory of the cell, which then translates the message into protein.

• Food science deals with the physical, chemical, and biological properties of foods as they relate to stability, cost, quality, processing, safety, nutritive value, wholesomeness, and convenience.
• Food science is a branch of biological science and an interdisciplinary subject involving primarily microbiology, chemistry, biology, and engineering.
• Food chemistry, a major aspect of food science, deals with the composition and properties of food and the chemical changes it undergoes during handling, processing, and storage.
• Food chemistry is intimately related to chemistry, biochemistry, physiological chemistry, botany, zoology, and molecular biology.
• Nonenzymic browning involves reaction of carbonyl compounds, which can arise from existing reducing sugars or from diverse reactions, such as oxidation of ascorbic acid, hydrolysis of starch, or oxidation of lipids.

Some chemical and biochemical reactions that can lead to alteration of food quality or safety

Introduction
The identification of agricultural crops with accuracy determines how precision farming works and affects biodiversity protection and food safety standards in addition to pest control practices. In modern agricultural practice, crop species identification relies on the combination of three traditional methods: morphological characteristics and biochemical markers, and remote-sensing technologies. These methods face a large variety of constraints such as environmental differences, high costs, and time-consuming operations. Genome-editing tools, especially CRISPR, a modern molecular identification tool for crops that increases efficiency and accuracy, have largely solved the problems traditional methods faced. Identifying agricultural crops correctly is an essential operation for resource management to be used efficiently in the hope that businesses meet regulatory standards and do their share in stopping trade-related fraudulent activities with incorrect labeling. The early and exact identification process helps pest and disease control because different crop species need different levels of resistance management (Rahayu et al., 2025). A reliable identification methodology remains important because sustainable agricultural practices and climate-ready crop varieties reach higher popularity in current times. The identification method based on morphological classification takes too much time and gets misdirected because of environmental conditions (Mascellino et al., 2024). High-resolution satellite data together with advanced computational tools needed for remote sensing-based identification cannot easily be accessed by small-scale farmers (Sharma et al., 2024).
The high specific identification of crop species becomes achievable through CRISPR-based technology which utilizes genetic markers as its primary feature. CRISPR enables fast and exact and affordable crop classification through its ability to detect and analyze specific genetic patterns. The accuracy of crop identification improves while real-time evaluation of plant diversity alongside disease. Alert traits becomes achievable through this method (Kanapiya et al., 2024). CRISPR-based biosensors have experienced recent advancements that enabled portable field-deployable identification tools which eliminate the need for complex laboratory setups (Lagener et al., 2025). Crop monitoring and breeding programs will undergo revolution through CRISPR technology integration in agricultural systems which will support the creation of climate-adaptive highyield crop varieties.
The evolution of CRISPR application research demonstrates its expanding function in farming efficiency together with food security establishment. The chapter explores CRISPR-based methods’ identification potential for crops using an evaluation of their superiority over conventional identification techniques and their expected contributions to future agricultural developments

Summary

The indiscriminate use of pesticides and their persistence in the environment enhances the residue levels in meat, dairy products, eggs and food crops, consequently adversely affecting the human health (Akhtar, 1985). In India millions of tons of pesticides are applied to the agricultural fields to increase the productivity for compensation of the nutritional requirements of increasing population from the same land resources. It was the advent of green revolution that really spurred the growth of pesticide industry. Several reports published reveal that a large amount of organochlorine pesticides is present in vegetables, fruits and soft drinks besides other pesticides compared to World Health Organization (WHO) standards (Martin and Duggan, 1968; Corneliussen, 1969; Ulmann, 1972; Hildebrandt et al., 1986). Organochlorine pesticide persistence, biomagnification and toxicity adversely affect the ecological systems (Khanna et al., 2002). There are a large number of different types of pesticides which differ in their mechanisms of action. Some pesticides are very acutely toxic in humans, while others have low acute toxicity but may cause long term health effects in chronically exposed individuals. Each pesticide class has to be considered individually when predicting effects. Therefore, considering organochlorine pesticides induced disruption of body functions and possibly the involvement of free radicals is cardinal to present study.

Introduction

Food is an important contributor to human well being and is essential for sustaining life. From this point of view food is expected to contains essential nutrients, such as carbohydrates, protein fat, vitamins and other essential minerals which support life. The food production initially started for individual sustenance which slowly developed in to a community requirement necessitating the involvement of man and machinery for various activities concerned with food. The progress from famine in many parts of the world to the level of self-sufficiency and the role of science and agriculture have been absolutely significant in many parts of the world. India is no exception and the green revolution during the post 1960s and the succeeding blue and white revolution ensured the self-sufficiency in India.

Of late food acts as a vehicle for inclusion of hazard in food meant for human consumption. According to Grunert (2005), factors responsible for the alarmingly higher rate of concern by public include significantly higher number of food scares in public domain, critical understanding of production and processing of certain food products sold in the market as well as the diversified food choices of the consumers in different parts of the world. Besides, the increased awareness of the consumers on food related health issues also have its share in the increased concern on food at large.

Presently food is considered as a commodity which crosses international border concurrent with the human movement and hence quality has become a major concern. The traditional food is getting replaced with value added products, particularly ready to cook, ready to eat, partially cooked products, etc. consequent to life style changes. In order to meet the demand of the trade, the conventional processing is getting replaced by community processing or industrial processing with increased number of players in the primary production, processing, transportation and other value chain activities prompting larger product control. In the process, consumers are exposed to a variety of products produced as business ventures in modern food production systems needing efficient quality control and quality assurance to tackle the issues of quality. The increased incidence of hazards in both raw materials and final products consequent to the absence or inappropriate production schedule calls for increased food safety concerns. Besides the shelf-life of the products, addition of chemicals during the production also add to the concern.

Maize is one of the most important cereal crops of the world. Being a C₄ plant, it is physiologically more efficient than rice, wheat and other important cereal crops which belong to C₃ category. In Punjab, traditionally, maize is grown during Kharif season but from last few years the area under spring maize is increasing because its cultivation is highly profitable and commercially viable after harvesting of potato crop. No doubt, Punjab has been the leading state in contributing to the national food security but simultaneously paddy-wheat rotation resulted in depletion of underground water, deterioration in soil health and environmental pollution. These problems need to be addressed for the sustainability of these natural resources and at the same time meeting the food grain production for the ever-increasing human population. Under such circumstances, maize crop is a viable alternative to replace some area occupied by rice and can help in breaking rice–wheat dominant cropping pattern in the state. Promising maize hybrids possessing high grain yield potential and offering comparable economic returns to the farmers are available with Punjab Agricultural University as well as with the private sector.

In Punjab, maize is commonly used in the form of chapatis, boiled or roasted green cob, breakfast food like cornflakes, popped grains etc. Maize chapatis are used as a delicacy with saag. The high carotene content of yellow grained maize is considered very useful in imparting yellow colour to egg yolk. Therefore, a substantial quantity of maize grain is utilized as an important ingredient of animal/ poultry feed industry. The use of maize grains for such purposes is going to increase as poultry and dairy industries are expanding. Maize plant is extensively used as green as well as dry fodder. The digestibility of maize fodder is better than sorghum, bajra and other non- legume forage crops. Maize plant does not have any anti quality factor hence can be fed to cattle at any stage. Maize can be utilized as an industrial raw material for number of industries viz., starch, ethanol, rubber, paper, cosmetics and pharmaceuticals etc. Quality Protein Maize (QPM) can be promoted for nutritional security of poor masses whereas pop corn, baby corn and sweet corn can help in increasing the farm income due to high market value. In Punjab, major industries which are utilizing maize are The Sukhjit Starch & Chemical Limited, Kapurthala, and M/s Field Fresh Food Pvt. Ltd, Ladhowal, Ludhiana.

Introduction

Many post-harvest treatments are applied to horticultural crops, either to maintain the quality (taste, colour, flavour, texture) or improve the visual appeal. Most important of these treatments are temperature management including the cold chain where the temperature of the crop is reduced rapidly and stabilize temperature after harvesting. Exposing the crop to high or low temperature and application of chemicals after harvest helps in managing/ prevent pest and diseases and sprouting occurrence respectively.

Harvested produce must be handled with care at every stage to avoid the mechanical damage and subsequent fungal/bacterial infection. Adopting appropriate post-harvest handling operation will minimize the all ill effects of post-harvest (Fig. 1).

Charts and graphs are essential tools for visualizing data and presenting information in a clear and comprehensible manner. They transform raw data into visual representations, making complex data easier to understand, analyze, and communicate. Various types of charts and graphs serve different purposes and are chosen based on the nature of the data and the message intended to be conveyed.

Historical Background and Evolution

The use of charts and graphs dates back centuries, evolving alongside advancements in mathematics, statistics, and technology.

Early Developments

Ancient Civilizations: Early forms of data visualization can be traced back to ancient civilizations. For example, Egyptian hieroglyphics and Babylonian cuneiform tablets included basic graphical representations of information.

William Playfair (1759-1823): Often considered the father of statistical graphics, Playfair introduced several fundamental chart types, including the bar chart, line chart, pie chart, and circle chart, in his works such as “The Commercial and Political Atlas” (1786) and “The Statistical Breviary” (1801).

Hormones are a group of biochemical messengers that assist in transmitting signals from one part of an organism to another. The term “hormone” was first coined by Ernest Starling in 1905 after the discovery of secretin, a chemical produced in the small intestine that regulates pancreatic secretions. In 1937 Went and Thimann defined hormones as “a substance which, produced in any one part of an organism, is transferred to another part and there influences a specific physiological process”. Since then hormones are also known as chemical messengers. These intercellular messengers are produced by specialized cells of endocrine glands, generally called effectors and released into the bloodstream thereby assisting in the regulation of physiological processes in target cells. Hormones assist in the regulation of a plethora of cell functions such as maintaining homeostasis, metabolism of biomolecules, growth, and reproductive function, and controlling blood pressure and glucose levels that are essential in orchestrating an intricate body- balance. The pituitary, thyroid, pancreas, adrenal, parathyroid, and gonads (testis and ovary in males and females respectively) produce hormones. Apart from these endocrine glands, few other organs such as the kidney, heart, and liver are also involved in hormone production. The pituitary gland