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(Colorimetric Method of Barker and Summerson, 1941)
I. Method for Blood / Plasma
Principle

In this method, glucose and other interfering material of the protein free blood filtrate is removed by the Van Slyke-Salkowski method of treatment with copper sulphate and calcium hydroxide. A part of the resulting solution is heated with concentrated sulphuric acid to convert lactic acid to acetaldehyde, which is then determined by reaction with p-hydroxydiphenyl in the presence of copper ions.

The term “nutraceutical” was coined from “nutrition” and “pharmaceutical” in 1989 by Stephen DeFelice, MD, founder and chairman of the Foundation for Innovation in Medicine (FIM), Cranford, NJ. According to DeFelice, nutraceutical can be defined as, “a food (or part of a food) that provides medical or health benefits, including the prevention and/or treatment of a disease”. In other words, nutraceuticals are chemical or natural feed ingredients which may enhance health by providing a physiological benefit by the provision of basic nutrients. Enzymes, prebiotics, probiotics, yeast and fungal extracts, predigested hydrolyzed carbohydrates and fats, phytogenic additives, acids etc are included in nutraceuticals. However, the term nutraceutical as commonly used in marketing has no regulatory definition.

Entrepreneurship opportunities in veterinary sciences have expanded significantly with the integration of technology. The convergence of veterinary expertise and innovative technological solutions has opened up new avenues for entrepreneurs to create impactful businesses in the field. This field offers a wide range of opportunities for persons passionate about animal health and welfare. In the recent years, the demand for high-quality veterinary services and products has increased tremendously, creating an enriching environment for aspiring entrepreneurs. There are various avenues available in the veterinary industry for individuals thinking about establishing their own businesses.

.1. Origin and distribution

• Originated from West Africa.

• Cultivated in Malaysia, Indonesia, USA, Cameroon, Cote d’Ivoire, Ghana, Liberia, Nigeria, Sierra Leone, Togo, Angola and Congo.

• Commercial scale plantation in Malaysia was found in 1917.

9.2. Economic importance

• Produces edible palm oil as well as palm kernel oil.

• Maj or constituent of oil is mono unsaturated oleic acid; rich in carotenoids.

• Unrefined palm oil is a significant source of tocotrienol, part of vitamin E.

• Oil content is 17 to 27% for palm oil, and 4 to 10% for palm kernels.

Need of Analytics in Fisheries Management

New technologies are introduced for industrial vessels every day: Fuel and gear sensors, cameras, electronic catch reports etc. There is a global push to track small-scale fishing vessel. In the coming years, fisheries monitoring centers will have to adjust from tracking a few hundred vessels to tens of thousands of vessels, meaning huge quantity of data. To make sense of all this information, it must be properly analysed. The future of fisheries depends on big-data analytics

This may help in many ways. Fishing trips could be tailored to meet quotas in the shortest time reducing fuel consumption and crew costs. Real-time catch data could help administrations close a fishing zone once quota is reached. Fisheries administrations can monitor catch efforts over an entire region and decide which zones to close, improving management of stocks and fishing licenses. Fighting the challenge of Illegal and Unreported fishing contributing to over-exploitation of fish stocks. Use of disruptive technologies in fisheries and aquaculture like block chains, sensors and automatic identification systems (AIS) has the potential to change the processes profitability and sustainability of the sector

Globally used popular Data Science Software

• MS-EXCEL

• SPSS (IBM)

• STATA • MINITAB

• SAS

• R

• PYTHON

In order to evaluate the correct status of the organs their correct disposition within the body becomes very essential. Although one cannot see the organs beyond the skin, but appropriate knowledge of the topography of the organs can act as a glass covered watch wherein one can see from outside the arms and the dial of the watch.

Horse

Heart: The heart is placed in the middle mediastinum of the thoracic cavity with its long axis directed caudo-ventrally. Three fifth of the heart lies on the left of median plane while two fifth is on the right of the median plane. The base is placed dorsally, with its highest point at the junction of the dorsal and middle thirds of dorso-ventral diameter of the thorax. As a whole it lies opposite to second intercostal space/third rib to sixth rib/sixth intercostal space. The apex of the heart lies in the center of the last segment of the sternum about half inch from the sternal part of the diaphragm. Heart is directly related to the lateral thoracic wall at the cardiac notches formed in the lungs. The left surface of the heart extends from third to sixth rib in the lower third of chest while the right surface extends from third to fourth intercostal space.

Fishes are used by human beings in different forms from time immemorial millions of human beings suffer due to hunger and malnutrition and fishes from a rich source of food, which has following importance:

1) Fish is an excellent food for mankind is more easily perishable than cattle, sheep and chicken.

2) Fish flesh is easily digestible by child and man.

3) Fish flesh has very great nutritive and medicinal values.

4) Fish flesh contains proteins, bass, vitamins, waters and minerals.

5) All the essential required amino acids are present in the fish flesh.

6) Fish flesh contains proteins about 13-20 % and it is easily digestible and nutritional.

7) Fish flesh is highly perishable commodity constituted as 60-80 % water.

8) Fish flesh contains about 1-8 % fats or oils and it is more important value of fishes.

Abstract

European red mite is a  serious pest of apple in Himachal Pradesh. Recent survey has shown that 80 per cent apple orchards are infested with this pest. As a result of continuous feeding fruit remained undersized, poor coloured and sour in taste. New acaricides fenazaquin (0.001%) and propergite (0.057%) proved highly effective Standard acaricides dicofol ( 0.037%) and insecticide endosulfan (0.07%) remained effective upto 15 days only.

NAME OF THE UNIT: 

    1.    Location of Seed Storage 
    2.    Date of Inspections/visit

The surface of the earth gets heated up during the day and gets cooled during the night causing diurnal changes in the top layers of the soil. Soil temperature is an important part of micro-environment, where plant roots grow and capture the water and nutrients and thus, it affects the plant growth and yield. During crop germination also soil temperatures play vital role. Since the heat regimes of these layers are governed by the soil temperature, the measurement of soil temperature becomes extremely important.

Introduction

At the Banana Research Station, Kannara activities focused on Musa germplasm conservation and evaluation are in progress. The aim of the project is to collect and conserve wild and cultivated germplasm representative of the diversity existing in the Musa genepool for evaluation and subsequent use in breeding programmes and to make available selected superior material to the farmers of Kerala. The center presently holds a Musa germplasm of 256 accessions in its field gene bank. These accessions have been characterized and evaluated as per INIBAP/IPGRI Musa descriptors and a database developed.

In a vegetatively propagated crop like banana, in vitro culture is widely employed in germplasm introduction and conservation and is an essential requirement for the safe movement of germplasm (Sharrock and Engels, 1996). By this method, germplasm material is readily propagated, thereby facilitating the dissemination of disease free plants. Since 2001, efforts have been made at Banana Research Station, Kannara to introduce forty new exotic accessions employing in vitro culture for conservation and evaluation. These are material from the global Musa germplasm collection maintained by International Network for the Improvement of Banana and Plantain (INIBAP), France and supplied by National Bureau of Plant Genetic Resources (NBPGR), New Delhi.

Etiology

Genus Mycobacterium contains many pathogenic species that can cause disease in birds (M. avium, M. tuberculosis, M. genavenes, M. gordonae). Within M. avium, organisms are divided into four sub species. M. avium sp avium, M.avium sp silvaticum, M. avium sp homnissuis and M. avium sp paratuberculosis. Mycobacterium avium sp avium - has wide host spectrum; poultry, pigeons, raptors, ratites and wild birds. M. genavense- affects Psittacines and passarines. M. tuberculosis- psittacines and other cage birds, and wild birds in captivity. M.bovis- Psittacines and others.

Under natural conditions, M. avium sp avium does not cause severe disease in mammals except in rabbit and pig. Majority of mycobacteriosis in pigs is by M. avium sp avium. In cattle and other mammal infection by M. avium remain localized and is known to give positive reaction to mammalian tuberculin. The disease in caged and wild birds in captivity is common with M. tuberculosis, because of its close contact with humans. Tuberculosis is a serious problem in zoological gardens. M. avium is being isolated in increasing number of immunocompromized human patients.

Crops are grown in soil. There are different types of soil. The soil type helps farmers decide what crops will grow best because some crops do better with particular types of soil. Testing the soil helps determine what nutrients are available or unavailable for growing certain crops. Farmers usually have local agricultural experts test the soil on their land for information on how to proceed in growing crops. The best soil has a balance of nutrients, such as potassium, magnesium, and others. Certain crops may need more of one nutrient, so farmers put back those ingredients into the soil for better crops.

Six different types of soil are listed below along with the crops which do well in these soils.

1.Loam: This type of soil helps grow the best possible crops because it provides the necessary elements for plant growth and development. Loamy sand contains mostly sand plus clay and silt. Such soils are good for root vegetables, such as carrots, radish, turnip, beets, leafy vegetables, potatoes and tomatoes.

2.Clay: Cabbage and broccoli grow well in clay soil, but it is not good for root vegetables because of its dense texture which does not allow free flow of water.

3.Sand: Root vegetables, such as turnips, parsnips, and carrots, grow well in sandy soil.

4.Silt: Silty soil is fertile and offers nutrients for plant development. Lettuce, cabbage, carrots, turnips, and many other vegetables flourish in silt. Strawberries, raspberries, blackberries do well.

5.Peat: Peaty soil provides plant growth when mixed with rich organic matter, lime, and compost that reduces its acidity. Legumes, root crops, cabbage, and spinach are easily grown in soil that’s well-drained.

6.Chalk: Sweet corn, beets, spinach, and cabbage are among the vegetables that benefit from chalkier soil. It is recommended that farmers use fertilizers and balance the pH levels to deal with the alkaline that can sometimes stunt growth.

Frequency Distribution is the arrangement of various values of a variable in order of magnitude either individually or in groups alongwith their corresponding frequencies or class frequencies. Frequency distribution is necessary for data condensation and revealing pattern within the data. Frequency distribution can be (i) Ungrouped Frequency Distribution (ii) Grouped frequency distribution.

In case ofungrouped frequency distribution, the individual values ofthe variable are arranged in ascending order alongwith their frequencies. Frequency is the number of times a particular observation is repeated.

In case of grouped frequency distribution, the values of variable are grouped into classes alongwith their corresponding class frequencies. Class frequency of a class is the number of observations lying within the upper class limit (maximum value) and lower class limit (minimum value) of the class.

Let us first illustrate the preparation of ungrouped frequency distribution. Consider 50 petridishes each containing 6 no.s of seeds for germination. Let the variable x_i denote the no. of seeds germinated in a petridish.

For RT-PCR, the extracted total RNA is first reverse transcribed to cDNA using reverse transcriptase enzyme. Following are the protocols for cDNA sysnthesis and RT-PCR.

Note: cDNA synthesis and RT-PCR can be performed according to the manufacturer protocol from where the specific reagents for the above methodologies are purchased.

8.1 Purpose

To lay down a uniform protocol for the synthesis of c-DNA from total RNA

8.2 Scope

The scope of this Standard Operating Procedure is to lay down a standard procedure for the synthesis of c-DNA from total RNA

Morphological characteristics

Straight bole, longitudinally fissured bark, imparipinnate and elliptic leaves, fragrant flowers in huge panicles, and winged, flat pods distinguish Pterocarpus species in the field. The tree can grow up to 30 metres tall and 2.5 metres wide, with a straight and clean bole. The bark is scaly, tough, and fissured longitudinally. Leaflets are five to seven in number, 8–13 cm long, oblong or elliptic in shape, or rotund in shape, with 15–20 pairs of lateral veins. Kino-gum is a fragrant, brittle, almost black oleo-resin obtained from tree trunks that is angular and glistering and comes in minute flakes..

Floral characteristics

Yellow flowers bloom in panicles that are 1–5 cm long and fragrant. Flat, orbicular, winged pods with a diameter of up to 5 cm. Seeds range from one to three in number, are bony, and have a convex form. Flowering starts in November, and fruits lasts until March.

Distribution

The tree can be found in central and peninsular India, primarily in dry mixed deciduous tropical forests in Gujarat and Madhya Pradesh, as well as sub-Himalayan tracts, at elevations of up to 1000 metres. Natural populations have plummeted, and there are hardly any fragile young saplings in the forest. Due to a lack of autogenic reproduction, this species is endangered.

10.1. Origin and distribution

• Native to the tropical Africa and grows in the Nepal, India, Bangladesh, Sri Lanka, Cambodia, Laos, Myanmar, Thailand, Vietnam, Malaysia, Indonesia and the Philippines.

• Palm is widespread in Tamil Nadu, Kerala, Andhra Pradesh, Telangana, Odisha, West Bengal, Bihar, Jharkhand, Karnataka and Maharashtra.

• In Tamil Nadu, it is present in Tuticorin, Tirunelveli, Thenkasi, Virudhunagar, Ramanathapuram Madurai, Theni, Salem, Namakkal, Kancheepuram, Chengalpattu, Tiruvallur, Villupuram, Kallakurichi and Cuddalore districts.

• Palm is dioecious in nature.

• Palmyrah was declared as “State Tree of Tamil Nadu” by the Government of Tamil Nadu.

Experimental Designs

Experimental Designs are used widely to compare more than 2 groups, commonly called as treatments. It is very common in agriculture to conduct several experiments, mostly in fields to test various varieties, different levels of inputs, etc. When one is interested in crop modelling, it is necessary to identify the values of several constants, such as input efficiency, growth periods, growth rates, etc. Instead of guessing these values, it would be more appropriate to conduct experiments to derive these values empirically, which can improve the modelling efforts. The principles of experimentation were given by Sir R.A. Fisher during 1920’s, which are still relevant and found in every scientific research namely, (i) Randomization, (ii) Replication and (iii) Local Control. The following sections introduce some of the commonly used terms in Experimental Designs:

Factor: Variables which are set/considered by the experimenters in any experiment. Examples of factors are: Nitrogen, Varieties etc. Level: The various values of a particular factor considered in the experiment. Examples of levels are: Rates of nitrogen applied, different varieties considered in the experiments.

Treatment: The levels of one or more variables (Factors) set/considered by the experimenters.

Note that in many experiments, the number of variables (i.e. factors) considered may be one only.

Fish are of great economic value for man. In addition to it is an important item of food and provides several important by – products also which are as :

1) Fish oil

The most important fish by product industry is fish oil which acts as a vehicle for fat soluble vitamins i.e. A, D, E and K, and source of essential faty acids.

The oil of fish is categorized into two main types as the fish liver oil and fish body oil.

The oil abstained from the whole body of fish is called as body oil and is poor in vitamin contents.

The oil abstained from the only liver of the fish is called as the liver oil, and is good quantity of vitamins.

The body oil is less valuable as compared to the liver of the fish.

Sapota or sapodilla popularly known as Chiku is native to tropical America where it is mainly grown for its chickle, “the gutta parcha” extracted from its latex from stems. It is used as a base material in chewing gum and in some other industrial uses. However, in India it is cultivated for its delicious sweet fruits. The fruit is fleshy berry, variable in shape, size and weight. The skin is thin, rusty brown somewhat scurfy looking like Irish potato, and the pulp soft, melting, crumbling with a sandy or granular texture with 1–5 hard black seeds. The fruit has an exceptionally sweet malty flavour.