eChapters

A. Answer the following.

Q.1.     Why the serum is inactivated at 56º C for 30 min before using it in CFT and VNT ?
Ans.     The complement and non-specific virus inhibitors are inactivated in this process.

Q.2.     What are the different enzyme and complement factors are secreted continuously by macrophages ?
Ans.     Lysozyme and Complement 2, 3, 4 and 5.

B. Write true or false about the followings.

Q.1.     The classical complement activation pathway is dependent on antigen-antibody complex.
Ans.    True.

Yield mapping or yield monitoring is a technique in agriculture of using GPS data to analyse variables such as crop yield and moisture content in a given field. This data produces a yield map that can be used to compare yield distribution within the field from year to year. This allows farmers to determine areas of the field that, for example, may need to be more heavily irrigated or are not yielding any crop at all. It also allows farmers to show the effects of a change in field-management techniques, to develop nutrient strategies for their fields, and as a record of crop yield.

Yield is ultimate indicator of variation of different agronomic parameters in different parts within the field. So mapping of yield and interpretation and correlation of that map with the spatial and temporal variability of different agronomic parameters helps in development of next season’s crop management strategy. Yield monitors measure the volume or mass flow rate to generate time periodic record of quantity of harvested crop for that period. Time periodic yield data is then synchronized with location address obtained from on-board GPS system to create most common colour coded thematic map. Yield mapping can be carried out easily in mechanized crops. By using either loading cells that weighed the crop passing on a conveying belt or an array of sonic beam mounted over the grape discharge chute to estimate the volume, and the tonnage, of fruit harvested. Tree crops seem to have more stable yields. In handpicked fruits yield mapping is more difficult. Yield maps are one of the most valuable sources of spatial data for precision agriculture. In developing these maps, it is essential to remove the data points that do not accurately represent the yield at a corresponding location. Map averaging or smoothing is usually done to aid data interpretation. A long yield history is essential to avoid drawing conclusions that are affected by the weather or other unpredictable factors during a particular year. Processed yield maps can be used to investigate factors affecting the yield or to prescribe variable rate applications of agricultural inputs according to spatially variable yield goals (yield potential).

Factor analysis: Factor analysis is the most commonly employed multivariate procedure in the research studies when there is a systematic interdependence among a set of observed or manifest variables and the research worker is interested in finding out something more fundamental or latent which creates this commonality.

Introduction

Rice plays a unique role in providing food to the majority of the world population. Rice is the most widely consumed food for nearly three-fourths of the population in India. Now a days scented rice is getting higher market price than normal rice varieties. Black Scented rice is commonly considered as forbidden rice. Different researches proved that black Scented rice is one of the “super food” of the world. Black Scented rice is consumed in various ways like puddings, pulao, bread, etc in different festivals and during important ceremonies. Mostly this crop is prone to lodging but resistance to infestation by insect and pest. Works done in India and abroad indicates that the particular method of establishment, adequate level of nitrogen and a particular time of application of nitrogenous fertilizer can enhance productivity. Nitrogen is the main nutrient that determines rice yield, due to its role in the photosynthesis, biomass accumulation and, spikelet formation (Hasegawa et al., 1994; Yoshida et al., 2006).

SOURCES OF POISON

1. Malicious poisoning

Unlawful or criminal killing of the animals by certain toxicants as arsenic, Insecticide, datura etc.

 2.Accidental    poisoning    
A) Natural sources 
 ? Plants - e.g. Lantana, datura etc.
 ? Animals - e.g. snake bite etc.
 ? Microbes - fungi (aflatoxicoses etc.), bacteria (Botulinum toxin) etc.
 ? Minerals - Se, As, fluoride etc.
 B)  Man made sources
 ? Agrochemicals - Insecticides, Herbicides etc.
 ? Industrial wastes - Mercury, Copper, Lead etc.
 ? Household products - disinfectants (phenol), Napthelene etc.
 ? Therapeutic agents - paracetamol in cats be lethal
 ? Food & feed - Genetically modified food (GM)
 ? Environmental pollutants & managemental practices

Though India is one of the major fish producing countries in the world, it has no history regarding technical and academic training in fisheries. India has to tackle simultaneously the long range problem of establishing a regular fisheries educational system as one of its fisheries policies for ensuring a reserve of large number trained persons. The short range problem of providing specialized training to the large number of in service personal, who were not basically qualified in all aspect of fisheries had been planned. Therefore, the government of India and the state Governments had put in considerable effort to solve problem of shortage of technical person as by organizing a number of fisheries training institutions in the country at different levels. A few universities are also providing training in fisheries leading to degrees in fisheries.

The following are the main fisheries training institutions which are giving special training in fisheries in India:

The silkworm chawki rearing is a vital stage in rearing as it has major influence on cocoon production.  Temperature, humidity and leaf quality are the three key important factors influencing chawki rearing.  Hence, maintaining these conditions at every step is very important and calls for provision of separate chamber. Any alteration in these factors will influence during later ages of silkworm rearing leading to crop losses.  Taking these factors into consideration the Government of India promoted the establishment Chawki Rearing Centres (CRCs) in private sector also.  These CRCs brush the silkworm eggs at one point under controlled conditions and distribute the worms to the needy farmers.
 
In recent years, bivoltine hybrids are becoming more popular in India and particularly in Karnataka.  This is due to the efforts of the Central Silk Board, Karnataka State Department of Sericulture and Japanese International Co-operation Agency.  These Bivoltines have high cocoon shell ratio (24 to 25%), better quality, fetches high cocoon price, better returns, high rendita (5.1 to 6.1) and raw silk percentage (20 to 22%) with filament length of 11502 to 12200 meters.  The farmers could achieve up to 70kgs cocoon yield per 100 Disease Free Layings (DFLs) and quality silk of 2A to 4A grade. 

Etiology

The subfamily Pneumovirinae includes two genera: Pneumoviruses and metapneumovirus. The later consists of both human and avian metapneumovirus (aMPV). aMPV produces turkey rhinotracheitis (TRT) in turkey and Swollen head syndrome (SHS) in chickens. aMPV has been detected in other species of birds, but the disease in those birds are not well defined.

Occurrence Species

A new disease of turkeys (in the UK since 1985) which is suspected to cause some lesser problems in chickens.

Violaceae

Morphological characteristics

Banafsha is a small perennial herb with a short semi-subterranean fleshy stem (rootstock).The older parts bear fibrous roots. The upper younger portion is marked with close circular scars of the leaves of the previous years, and gives off elongated, rather fleshy, leafless runners. Leaves are ovate and crenate–serrate.

Floral characteristics

Flowers are borne on long, slender, axillary stalks (scapes) exceeding the leaves. Flowers are lilac, blue, white or purple in colour and borne on the main stem, the larger ones being 6–12 mm in diameter. The flowers arising from the axils of the cauline leaves are minute. The cleistogamous (underground) flowers measure about 1 mm across. Fruit is a few seeded, globose capsule. Flowering occurs during February–March, while fruiting occurs during April–May.

When embarking on the entrepreneurial journey, it’s essential to craft a Unique Value Proposition (UVP) that sets your business apart in a crowded market. Your UVP should succinctly convey the distinctive benefits your product or service brings to customers, solving their pain points in ways competitors can’t. To develop a compelling UVP, start by delving into your target audience’s challenges and desires. Understand their needs deeply and determine how your offering can address those needs uniquely and effectively. Whether it’s through innovative features, exceptional quality, or a unique approach, your UVP should highlight what makes your solution stand out. Distill this essence into a clear and memorable statement that resonates with your audience.

Rice is the longest continuously grown cereal crop in the world and according to the International Rice Research Institute (IRRI) it is “one of the most important developments in history” (Forssell, 2008). India has to produce 114 million tons of rice by the year 2030 to meet the food grain requirement of burgeoning population. In state Bihar, Kharif is the predominant season with rice cultivated in 3.2 m ha. On the other hand the rice is cultivated in about 1 lakh ha during Rabi season (Anonymous, 2014). The process-based dynamic simulation crop models based on soil, crop and weather factors could be effective research tools for planning alternative strategies for crop management, land use and water management and also a useful tool for planning and developing technological interventions in diverse areas like India (Aggarwal and Kalra, 1994).

3.1 Introduction

Eggs are composed of three main parts; the eggshell with the eggshell membrane, the albumen or white, and the yolk. The yolk is surrounded by albumen, which in turn is enveloped by eggshell membranes and finally a hard eggshell.

3.2 Eggshell

The eggshell is composed of a foamy layer of cuticle, a calcite or calcium carbonate layer, and two shell membranes. The ultrastructurally the eggshell includes shell membranes, mammillary zone, calcium reserve assembly, palisades, and cuticle, with 7,000 – 17,000 funnel shaped pore canals distributed unevenly on the shell surface for exchange for water and gases

The concept of single cell micro-gel electrophoresis (SCGE)/comet assay is f irst introduced by Ostling and Johanson (1984) as a method to measure DNA single-strand breaks under neutral conditions. Singh et al. (1988) modified this technique by using alkaline conditions (>13 pH) of electrophoresis, which enabled detection of not only frank strands breaks but also alkali labile sites, DNA cross- linking and incomplete excision repair sites. Now-a-days, this technique is extensively used to study the genotoxic effects of various chemicals. The advantages of SCGE technique include: (1) collection of data at the level of individual cell, allowing for more robust types of statistical analysis; (2) need for small number of cells per sample (<10,000); (3) its sensitivity for detecting DNA damage; and (4) that virtually any eukaryotic cell population is amenable to analysis. The idea is to combine DNA gel electrophoresis with fluorescence microscopy to visualize migration of DNA strands from individual agarose-embedded cells. If the negatively charged DNA contained breaks, DNA supercoils are relaxed and broken ends are able to migrate toward the anode during a brief electrophoresis. If the DNA is undamaged, the lack of free ends and large size of the fragments prevented migration. Determination of the relative amount of DNA that migrated provided a simple way to measure the number of DNA breaks in an individual cell.

Genotoxic effects of any xenobiotics in PMNC and splenocytes of Wistar rats can be studied according to the working protocol of Dhawan et al.

A family of about 175 genera and 2,500 species, Amaranthaceae are mostly distributed in tropical but also in temperate regions. About 18 genera and over 50 species have been reported from India. Some of the larger genera with the number of their approximately reported species and common names are Alternanthera (200, alligator weed), Gomphrena (100, globe amaranth), Iresine (80, gizzard plant), Amaranths (60, pigweed) and Celosia (60, cock’s comb).
Gomphrenas are 30-40 cm tall, bushy with erect stems, hairy, light green with oblong-ovate leaves tapering towards the base. Round button like or spherical flower head is a cylindrical spike 1-2 cm long and 1 cm in diameter and may be terminal and/or axillary, and are born on long, thin stems above the foliage. Each flower head has tiny, conspicuous, papery, colourful bracts which enclose the inconspicuous true flowers. Bracts ovate, three mm long, apex acuminate, bracteoles 4.5-5.5 mm long. Ovary is sub-globose with two stigmas. Seed is brown, lustrous, 1.5 mm long (Wagner et al., 1999). It has both C3 and C4 pathway.
Selected Indian Genera
Achyranthes, Alternanthera, Amaranths, Celosia, Digera, Gomphrena and Pupalia.

Food security of a country can be achieved through advanced agricultural research, followed by efficient and effective transfer of technologies. Unlike laboratory experiments, agronomic experiments are being conducted at open environment which leads to lot of variations in outcome/results due to influences of unpredictable weather condition and heterogenic soil characters even though proper adoption of local control. Results of an experiment can be expressed in different ways to make the clients to understand easily recordable output data viz., growth attributes, yield parameters and yield, laboratory analyses data like soil and crop nutrient status and quality parameters of the produce. Nutrient management studies should focus the efficiency of particular fertilizer nutrient over others, methods of fertilizer application, contribution of nutrients to crops from soil and fertilizer etc. Similarly, water management experiments would explain the water conveyance, application and storage efficiencies, suitability of method of irrigation, water saving techniques and water use efficiency of different crops as well as agronomic practices etc. In weed management experiments, the efficiency of particular herbicide in terms of controlling weeds, and increasing crop yields have to be expressed by various efficiency indices. Weed suppression ability of an intercrop can be explained by smothering efficiency.

Introduction

National Commodity & Derivatives Exchange Limited (NCDEX) is a professionally managed on-line multi commodity exchange offering commodity futures trading in a wide range of commodities viz, agricultural, base metals, precious metals, energy, ferrous metal, and others. The shareholders of NCDEX comprises of large national level institutions, large public sector bank and companies. Promoter shareholders of the Exchange include ICICI Bank Limited (ICICI), Life Insurance Corporation of India (LIC), National Bank for Agriculture and Rural Development (NABARD) and National Stock Exchange of India Limited (NSE). Other shareholders are: Canara Bank, Punjab National Bank (PNB), CRISIL Limited, Indian Farmers Fertiliser Cooperative Limited (IFFCO), Goldman Sachs, Intercontinental Exchange (ICE) and Shree Renuka Sugars Limited. NCDEX is the only commodity exchange in the country promoted by national level institutions. This unique parentage enables it to offer a bouquet of benefits, which are currently in short supply in the commodity markets. The institutional promoters and shareholders of NCDEX are prominent players in their respective fields and bring with them institutional building experience, trust, nationwide reach, technology and risk management skills. 

ILT is an acute viral disease of chickens, pheasants and peafowl characterized by marked dyspnea, coughing, gasping and expectoration of bloody exudate. The disease is worldwide.

Hosts

Chickens particularly susceptible; reported to occur in pheasants and peafowl.

Age Range

All ages susceptible but is most often found in adult (mature or nearly mature) commercial chicken layers.

Blood Sample collection

Blood samples (5 ml per goat) will be collected aseptically from the jugular vein in a vacutainer tube (B.D. Bioscience, Germany) containing EDTA as anticoagulant and transferred to the laboratory in an ice box.

Isolation of Genomic DNA

The Genomic DNA will be isolated from the blood samples by ‘Phenol: chloroform isolation’ method as described by Sambrook and Russel (2001). The steps for isolation of genomic DNA are as follows: