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Bioinformatics is a recently emerged frontier discipline, which helps in analyzing biological data with the help of information technology and statistical software packages. In a broader sense, bioinformatics is the application of computer technology in management of biological information. This interdisciplinary scientific tool having no barriers between different branches of science like biology, biotechnology, mathematics, computer science and information technology, has great influence on advanced bio-science research, particularly dealing with genes, proteins and cell biology.

Bioinformatics is the branch of study that deals with the use of information technology (IT) for managing data of biological science. Bioinformatics drew public attention with the announcement of the draft sequence of human genome by the then US President Bill Clinton along with Craig Venter and Francis Collins in 2000 (Uma and Sathyanathan, 2004).

I. FILL UPS

1. _____ is the step in which the seeds are allowed to attain enough water holding capacity facilitating separation.
2. _____ process helps to draw the precise difference between filled live and filled dead seeds in IDS method.
3. PREVAC stands for_____.
4. NIR stands for_____.
5. IDS stands for_____.

Abstract

The two most important tasks for a patentee to undertake after being granted a patent, in any patent regime, would be to pay the renewal fees necessary to keep the patent alive, and provide proof of having worked the patent in the jurisdiction. This article seeks to highlight features of India’s legal requirements with regard to the working of patents on a commercial scale. The number of patents granted in a country is an indicator of the technological strength of the country. However, it should be noted that actual technological growth depends upon the working of patents. Working of a patent means the commercial exploitation of the invention that is embodied in the patent.

It is an acute disease of pheasants, chukars, partridges, turkeys, ducks, pigeons and wild birds. The viruses of EEE and WEE are of public health importance as they infect humans and occasionally cause fatal disease. The disease is further significant because of its link to disease in horses.

Etiology

The arboviruses are grouped into 12 different families. The five arboviruses causing disease in birds are Eastern equine encephalitis (EEE) virus, Western equine encephalitis (WEE) virus, Highland J (HJ) virus, Israel turkey meningoencephalitis (IT) virus and West Nile Virus WNV. The first three are in Togaviridae, and IT and West Nile Virus (WNV) are in Flaviviridae.

9.1. Downy mildew of Opium poppy
Pathogen: Peronospora arborescens (Berk) de Bary

Geographical distribution and losses
This is one of the serious and widespread diseases of opium poppy. The disease was observed epiphytotic in Argentina, Germany, Iran, Hungary, Austria and India. The disease was first recorded in India in 1918 and with the introduction of susceptible varieties, the disease rapidly became serious due to favourable environment in its cultivation area in India. Downy mildew now occurs in most regions in India (Rajasthan, Madhya Padesh and Uttar Pradesh). Downy mildew caused 20-30% loss of yield of opium latex. The secondary infection of downy mildew was found to reduce 11-22% latex.

Symptoms
The infection spread upwards from lower leaves. The entire leaf surface is covered by downy mildew coating, which is composed of sporangiophores and sporangia of the pathogen (Fig 9.1). The stem, branches and even capsules are attacked resulting in the premature death of the plants. The infection start from the moment the seed germinate and persist during the whole period of growth (Yossi fovitch 1929). Khristov (1943) reported that the pathogen often cause abnormal development in opium poppy. The pathogen also causes hypertrophy and curvature of stem and flower stalks.

The relative long periods of time required for completion of germination tests delays the seed marketing. This necessiated the development of rapid methods for estimating the germination capacity of seeds. This test was developed by Lakon (1942) in Germany.

1. PRINCIPLE

It is a biochemical test, in which living cells are made visible by reduction of an indicator dye. The indicator used is 2,3,5 triphenyl tetrazolium chloride. Within the seed tissues, it interferes with the reduction processes of living cells and accepts hydrogen from the hydrogenases. By hydrogenation of the 2,3,5-triphenyl tetrazolium chloride, a red stable and non diffusible substance, triphenyl formazan is produced in living cells (Fig. 1).

3.1. Seed generation system

Seed multiplication is nothing but production of a particular class of seed from specific class of seed up to certified seed stage.

• Drought resistant, Salt tolerant and hardy temperate fruit crop
• Highly perishable fruit
• Vitamin A 2600IU
• Thiamine : 217mg/100g
• Wild apricot or Zardalu has originated from India.
• Popular training system – Open vase system
• Apricot bears on spurs and laterally on 1year-old shoots.
• Apricot spur life – 3-4 years
• Ideal thinning agent – NAA @25-50ppm, 20 days after fruit set
• Ideal stage for freezing, canning and drying fully ripe fruits
• Chaubattia Alankar – Kaishu × Charmagz – Low chilling and early ripening
• Chaubattia Madhu – Turkey × Charmagz – Early ripening
• Chaubattia Kesri – St. Amrbois × Charmagz – mid season variety
• Low chilling subtropical varieties – New Castle, Early Shipley, St. Ambroise, Kaisha.

Introduction

Women entrepreneurship in economic development of any nation has been recognised for its significant contribution. The women folk can be considered as backbone of any nation. Now time has come for women empowerment. In rural Odisha, women not only play an important role for agricultural development but also plays significant role in allied agricultural sector such as dairy, fishery, poultry, mushroom production etc. In Indian condition dairy has been considered as “Rural Banking” as it is practiced mostly by majority of the marginal, small and landless farm families for their sustenance and livelihood. The role of rural women for animal husbandry is of paramount importance. In this sector they are managers as well as decision makers. Women do more than 80% dairy operation like cleaning, feeding, milking, marketing of milk and milk products. The state and central government initiate several scheme, programmes and projects for empowerment of women through various micro enterprises including dairy. Against this background a study was undertaken to identify the constraint faced by women entrepreneur in dairy sector in Khordha district of Odisha.

• General considerations

 • Volume

 • Colour

 • Reaction

 • Concentration of spermatozoa

 • Motility

 • Live and dead count

 • Microbiological examination

Key Points

• Neonatal diarrhea can be lethal without treatment.

• Administer aggressive fluid and electrolyte therapy to address newborn diarrhea.

• Fecal examination and/or culture findings can help control diarrhea.

• Ringer lactate is the preferred fluid.

• There is a high association between animal age and the presence of the causal factor for diarrhea.

Introduction

Diarrhea is one of the symptoms of a variety of medical problems. Diarrhea is characterized by an increased frequency of defecation and stools of varied consistency, which may have an increased concentration of water or a decrease in dry matter content. Increased peristaltic movement causes an increase in caudal flow of ingesta/GIT content, resulting in a decrease in intestinal transit time and thus diarrhea. Diarrhea is lethal in children; therefore, preventing f luid and electrolyte losses prior to diagnosis is critical to saving lives.

Plants play an important role in maintaining ecological balance by actively participating in the cycling of nutrients and gases like carbon dioxide, oxygen and air pollutants. Sensitivity and responses of plants to air pollutants is variable. Some plants are sensitive and act as bio indicators of air pollution (Kozhouharov et. al. 1985, Guderian, 1998). Others which are tolerant may act as sink to air pollutants. However this aspect needs more investigation for their use as indicators of air pollutants and in abatement of air pollution.

The compliance with hygienic and aesthetic standards in the dairy factory is, in the final analysis, dependent on the human element. Each employee, from top management to the most junior operative, has his role in the factory sanitation programme and carelessness at any stage may cause quality deterioration of products or endanger the health of consumers. Legal action against the dairy may also result from neglect of hygiene. Personnel hygiene refers to the personal cleanliness and related habits of the workers. Milkborne infections frequently originate from food contaminated by workers through their hands, breath, hair, perspiration, pimples, infected wounds, coughs, and sneezes. Transfer of even traces of human and animal excreta by the workers is a potential source of pathogenic microorganisms. Foodborne illness is known to originate from workers who were ill and practiced unsanitary food handling methods or poor personal hygiene. Even the healthy worker, who does not exhibit the symptoms of the disease, may harbour food poisoning bacteria in the respiratory or intestinal tract. It is always desirable, as a matter of practice, to look at different parts of the human body in terms of potential sources of bacterial contamination. Provisions regarding personal hygiene of employees are contained in all legislation dealing with milk treatment.

Early research in field of semen storage and delivery systems was conducted in USA. The straw technique for the storage of frozen semen was evolved by work of French, Danish and German workers whereas the pellet system of storage of semen was devised by Japanese scientists. The different packing systems of frozen semen are:

    9.1    Ampoule method
    9.2    Pellet method
    9.3    Straw method

9.1 Ampoule Method
In this method the semen is placed in a glass ampoule and is subsequently sealed and frozen in liquid nitrogen. The ampoule is thawed just before insemination and is then cut open. The semen is withdrawn into glass catheter and used for AI. Since the ampoule is sealed there is no risk of contamination during storage. The relevant details can be marked on the ampoule.

ABSTRACT

The cry1A genes of Bacillus thuringiensis (Bt) are widely used to engineer lepidopteran insect resistance in crop plants including horticultural crops. Continuous exposure of a single kind of Bt toxin can lead to resistance development in insect pest. Due to the difference in structure and unique insecticidal mechanism, cry2A genes are promising candidates for management of resistance development. Since India is rich in biodiversity and genetic resources, indigenous Bt strains are valuable tool for identification of novel cry genes. The present study describes the cloning of cry2Ab from a new indigenous strain of Bt, 14RI. The presence of cry2Ab gene in Bt strain 14RI was confirmed by PCR with primers specific to its internal region. Primers corresponding to the flaking region of cry2Ab were used for amplification of cry2Ab orf from the Bt strain 14RI. The amplified cry2Ab orf was first cloned into the T/A cloning vector, and then fused to the downstream of the cry2Aa promoter along with its orf1 and orf2 in the Bt shuttle vector pHT1301. Acrystalliferous Bt strain 4Q7 has been transformed with recombinant pHT1301 to express the cry2Ab of Bt strain 14RI.

1. FIELD OF APPLICABILITY

Grow-out test is the official measure for controlling the genetic purity of the seed lot. It serves as ‘pre-control’ as well as a ‘post control’ test for avoiding genetic contaminations. According to the official regulations in India, it is prerequisite for seed certification of hybrids of certain species such as cotton, castor, musk melon and brinjal.

The test is conducted for checking the sellers label with respect to genetic purity status of the seed lot under the provisions of the Seeds Act 1966. In addition, grow-out test can also be used as a measure to judge the efficacy of the certification agency or the inspector.

Agricultural policies in the past have witnessed a series of iterative changes following the economic reforms during the 1990s that marked a Division of Agricultural Economics. Though many of the reform processes were not initiated to directly affect the agriculture sector, it was affected indirectly (Chand, 2004). The mounting stock of food grains has partly been due to the weak purchasing power of the poor in the country. Nevertheless, the problem associated with buffer stock management and degradation of natural resources in some regions has triggered a debate to redefine the agricultural policies. As a remedial measure, it has been suggested that India should diversify its agriculture and get a foothold in the world market (Radhakrishna and Reddy, 2004).

• Native to temperate regions of Northern Hemisphere.
• Chilling requirement – 2000-2700 hr, highest among temperate fruits.
• Heavy rainfall during flowering causes – Blossom wilt.
• Heavy rainfall during ripening causes fruit cracking.
• In Europe, a wine – ‘Kirschwasser’ is distilled from the pulp of cherries.
• Most of the commercial varieties of cherry are self-sterile.
• Seedling rootstock – Pazza, Mahaleb, Mazzard
• Clonal rootstock – Colt, Mazzard, F-12/1
• Cherry fruits are the first to appear in the market among temperate fruits.
• Cherry has more calorific value them apple.
• Donar vars: Stella, Vista, Vic, Seneca, Vega.

Introduction

Agriculture and allied sectors have always been the backbone of the Indian economy and despite concerted industrialization in the last six decades agriculture still occupies a place of pride. The significance of agriculture in the national economy arises from the role it plays in India’s national income, employment generation and export earnings; this can be seen from the provisional estimate on contribution of various sectors to the GDP in the f irst quarter of 2020-21, particularly during COVID-19 pandemic, positive percentage change (+3.4) over previous year when all other sectors have negative change.

Introduction

BOGL had set up its wholly owned subsidiary company Bikas Oil Inc. (BOI), Moscow, Russia. Mr. Atanu Saxena and Mr. Samish Batra were recently selected for being deputed to BOI. Being a foreign location, this posting was considered as monetarily rewarding and good for career growth of employees. Subsidiaries of other oil and gas companies were also present in Moscow as this location was considered as the oil and gas business hub of Russia.