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Spectrophotometry measures how much a chemical substance absorbs light by measuring the intensity of light as a beam of light passes through the sample solution. Chemical compounds normally absorb or transmit light over a certain range of wavelength. Hence spectrophotometry is used to measure the amount of a known chemical substance. It is one of the most useful methods of quantitative analysis in the laboratory and is widely used.

A  colorimeter or spectrophotometer is an instrument that measures the amount of photons (the intensity of light) absorbed after it passes through the sample solution. With the spectrophotometer, the amount of a known chemical substance (concentrations) can also be determined by measuring the intensity of light detected. Depending on the range of wavelength of light source, it can be classified into two different types, namely,1) UV-visible spectrophotometer which uses light over the ultraviolet range (185-400nm) and visible range (400-700nm) of electromagnetic radiation spectrum and 2) IR spectrophotometer which  uses light over the infrared range (700-15000nm) of electromagnetic radiation spectrum.

Interaction of radiant energy, exhibiting the properties of both waves and particles, with matter is dealt in spectroscopy. Matter may be defined as material, existing in solid or liquid or gaseous phase, which is composed of molecules, atoms, or ions. Atoms, molecules, and ions, irrespective of their physical state, remain in constant motion in a matter. Molecules may rotate, vibrate, and move from place to place in space. While radiant energy interacts with matter, these molecular motions are influenced to some extent. It is observed that radiations from ultraviolet and visible range of electromagnetic spectrum lead to the movement of bonding electrons from lowest energy state (ground state) to higher energy state (excited state) in the molecules, while absorption of IR radiation causes the molecule to vibrate more. Such changes, referred to as transition, are responsible for changing the molecular energy level.

Speculation and hedging are important ways of minimizing price risk in business.

Speculation: Risk is taken by person specializing in business without much consideration of business trends.

Hedging: A calculated risk is taken.

Time, Speed and Distance

This section consists of problems on calculating speed, distance, time, head start and relative speed etc.

Introduction
The increase in the world population coupled with climatic fluctuations such as drought, floods, and high temperatures poses a serious threat to food security (Ray et al., 2013). Many researchers have quoted the importance of enhancing the genetic gain of primary crops at a faster rate to meet global food demands (Lin et al., 2016). It remains a challenging task for plant breeders to evolve resilient varieties in a shorter period by employing conventional approaches. The slow progress in crop improvement is mainly attributed to long breeding cycles/generations (Samantara et al., 2022). To overcome the drawbacks involved in traditional methods and to safeguard food security, speed-breeding methods are now being adopted at large or small units to achieve rapid genetic gains in many crop species. The speed breeding techniques include the use of controlled environments with manipulation provisions for light duration, intensity and temperature. This approach is more advantageous for helping plant breeders hasten crop development in several major photosensitive crops (Singh and Janeja, 2021).
Breeding a new crop variety via a conventional approach requires the selection of complementary parental genotypes with desired traits, followed by crosses and a series of selections and advancements of superior progenies to release candidate cultivars that meet market demands (Shimelis and Laing, 2012). Notable breeding goals in crop cultivar development programs include increased yield potential and nutritional quality and enhanced tolerance to biotic and abiotic stresses (Breseghello and Coelho, 2013). In any crop improvement program, the following breeding procedures can be distinguished in the order presented: (a) selection of desirable parents with complementary traits to be combined; (b) crosses involving the selected parents and the development of progenies; (c) selection and genetic advancement of the best progenies based on target traits; (d) selection of the best progenies for screening in multiple target production environments to identify the best performing and stable candidate cultivars; and (e) cultivar registration and seed multiplication and distribution to growers (Shimelis and Laing, 2012). These conventional breeding procedures are used in most crop cultivar improvement

Conventional crop breeding requires a substantial investment of time, space, and resources for the selection process and the subsequent crossing of desired plants. The time taken for the complete seed-to-seed cycle is a critical constraint in the advancement of plant research and breeding. The concept of speed breeding holds the promise of diminishing the duration necessary for the entire process of cultivar development, from creation to introduction and eventual commercial availability, thereby enhancing the improvement of both food and industrial crops. It revolves around optimizing conditions such as light intensity, temperature, and daylight duration (22-hour light, 22°C day/17°C night, and high light intensity). This approach enhances photosynthesis, triggering early flowering, and, in combination with yearly seed collection. The adoption of speed breeding shows promises in realizing nutritional security and promoting sustainable agriculture for the future rising population. The technique’s integration with advanced genomics and gene editing opens doors to precise trait incorporation. Moreover, speed breeding reduces resource consumption, aligning with sustainability goals. As local adaptations become feasible, it empowers diverse regions.

Aim: To perform speed control of D.C. shunt motor by

1. Armature resistance control method.

2. Field (flux) control method.

This method is based on the fact that by varying the voltage available across the armature, the back EMF and hence the speed of the motor can be changed. This is done by inserting a variable resistance RC (known as controller resistance) in series with the armature as shown in Fig. (a).

Sperm abnormalities may be genetical or morphological. They are directly related to the male infertility. • The wet mount method and phase contrast microscopy facilitate sperm visualization for abnormalities. Semen stained with vital stain can also be used for evaluating sperm abnormalities. Sperm morphology or related abnormalities can be evaluated by using eosin-nigrosine stain, carbolfuschin, aniline blue-eosine etc. • Generally, for head and tail abnormalities rose Bengal and William’s stain can be used while for acrosome and head abnormalities Giemsa stain can be used. Categorization of Sperm Abnormalities 1. On the basis of site of abnormality on the spermatozoa • Head abnormalities e.g. microcephalic head, macrocephalic/giant head, narrow or short sperm head, pyriform head, detached glea capitis (acrosomal cap), detached head and double head etc

13.1 The Normal Sperm Morphology

A mature spermatozoa is an elongated cell consisting of a flattened head containing the nucleus, a neck and a tail containing the apparatus necessary for cell motility. The sperm cell consists of the following parts covered by the plasmalemma or plasma membrane: 
    
13.1.1    Head
    
13.1.2    Neck
    
13.1.3    Tail / flagellum – The sperm tail consist of     
    
a.    Mid piece
    
b.     Main piece / Principal piece
    
c.     End piece

Sperm Plasma Membrane

The sperm plasma membrane is three layered membrane. It lies in close contact with the underlying cytoplasm and attached at three places i.e. over anterior outline of the sperm head, around the base of the head, in circle around the distal end of the midpiece (Jensen’s ring). This attachment causes creation of three compartments with probably no communication with each other. The sperm plasma membrane is invisible under normal light or phase contrast microscopy but can be visualized after addition of substances with light refractory index e.g. bovine plasma albumin or synthetic polysaccharides. It is very easily permeable to water but there is low permeability for inorganic cations (Na, K, Ca etc.) and even lower permeability for anions (NO3, Cl). Exposure of sperm cells to hypertonic media will cause the water to go from sperm to the surrounding media leading to shrinkage of sperm cell whereas exposure of sperm cells to hypotonic media will induce influx of water causing swelling of the sperm cell. The more water that goes into the tail the more it changes its shape and becomes rounded to give room for increasing amount of water. As the length of the tail decreases as it becomes round, the tail fibrils have less space and tail bends and coils within the membrane vesicle. Tail fibrils are not damaged initially and will continue to contract till the critical volume of bull sperm is 3.8-3.9 times of its volume in an isotonic solution. The swelling and change in shape of the spermatozoa are momentary processes and can be corrected if hypotonicity of the media is corrected before the sperm cells reach the critical volume for rupture.

Sperm membrane is responsible for maintenance of the intracellular biochemical environment. Damaged plasma membrane is responsible for the leakage of electrolytes and organic substances such as enzymes and may also lead to damage of sperm cells by foreign substances from the surrounding media.

Semen • Semen, a fluid released from the male reproductive system during ejaculation, is made up of a liquid phase called seminal plasma and a cellular component called spermatozoa. • The term “spermatocrit” refers to the percentage of spermatozoa(v/v) in semen, which varies from less than 2% in pigs to more than 30% in sheep. It is about 10% in bull. Semen= Spermatozoa + seminal plasma Spermatozoon • In 1677, the scientist Van Leeuwenhoek first discovered the spermatozoa and called them ‘‘animalacula’’. • Spermatozoa or sperm cells or male gametes, develop within the wall of the seminiferous tubules as a result of a complex process known as spermatogenesis. • The spermatozoon is a highly specialized cell that performs the sole function of fertilizing an oocyte.

The spermosphere represents a short-lived, rapidly changing, and microbiologically dynamic zone of soil surrounding a germinating seed. It is analogous to the rhizosphere, being established largely by the carbon compounds released into the soil once the seed begins to hydrate. These seed exudations drive the microbial activities that take place in the spermosphere, many of which can have long-lasting impacts on plant growth and development as well as on plant health.

Abstract

Spices are aromatic substances of plant origin which are commonly used for flavouring, seasoning and imparting aroma in foodstuffs. They are cultivated in an area of 5.98 million ha globally with a total production of 7.31 million tons. India is considered as the home of majority of these spices from ancient times and sizable area of the country is under spice cultivation. The latest statistics shows that the total cultivated area in India under spices is 2.89 million ha with a total production of 3.33 million tonnes. About 109 plants belonging to 31 families are recognized as spices useful as ingredients in food. Many of them have been demonstrated to mediate therapeutic benefits for wide spectra of diseases ranging from multiple sclerosis to colorectal cancer.

Introduction

Advances in molecular genetics in the last 20 years like rapid DNA amplification techniques using PCR, rapid sequencing methods and computational software programs have enabled the placement of molecular markers on to the maps of chromosomes of most major crop species and the subsequent tagging of genes of interest by their placement near those markers^[38]. Unfortunately very little or scanty molecular data of spices are available in the public domain (Table 12.1). ‘Orphan crops’ like spices which have not yet received the investment of research effort or funding can exploit comparative genomics and bioinformatics to assist research and crop improvement provided they are related to a well-characterized model plant species.

Black pepper is popularly known as ‘King of spices’, it is the dried berry of the pepper vine, so important in the International Trade Market. The crop has its origin in the tropical rain forests of Western Ghats of India.  Kerala is the leading State in the production of black pepper with the ubiquitous presence of this crop in coconut, arecanut and coffee plantations. Chief pepper producing countries are Indonesia, Brazil, Malaysia, Cambodia, Sri Lanka and Vietnam.

Spices are those plants, the products of which imparts strong flavour and aroma to the food. This is attributed to the presence of compounds such as volatile oils present in the plant parts. India is renowned for wide variety of spices and is designated as land of spices. Spices are indispensable ingredient in almost all Indian dishes and cuisines foreign invaders invaded India for spices in ancient times. India is figured as the largest producer, consumer and exporter of spices and spice products in the world. Turmeric and Ginger are the major rhizomatous spices of vegetable origin widely cultivating and using in India. They are known for its antimicrobial and antioxidant properties, hence using in medicines for the treatment of various ailments. Apart from these rhizomatous spices, India is also contributing a major share in the production of seed spices. Around twenty spices among sixty-three spices growing in our country are classified as seed spices. Major seed spices of India are coriander, cumin, fennel, fenugreek, dill, ajowain, celery, anise, nigella, caraway etc. These seed spices are extensively cultivated in the arid to semi-arid region of India as Rabi crop.

It is estimated that, spice production in our country increased from 67.64 lakh tonnes in 2014-15 to 106.79 lakh tonnes in 2020-21 with an annual growth rate 7.9%. There is a corresponding increase in the area of cultivation also. An increase in area from 32.24 lakh hectare to 45.28 lakh hectare was observed. Among the major spices, cumin (14.8%), garlic (14.7%), ginger (7.5%), fennel (6.8%), coriander (6.2%), fenugreek (5.8%), red chilli (4.21%) and turmeric (1.3 %), showed significant growth rate in production. The export of spices contributes around 41% of the total export earnings from all horticulture crops in the country and it ranks fourth among agricultural commodities, after marine products, non-basmati rice and basmati rice.

INTRODUCTION
To turn raw spices into usable products like ground powders, whole spices, or blended mixes, a number of processes are involved in spice processing. The procedure guarantees that the spices are safe to eat and appropriate for longterm storage in addition to preserving their flavor and aroma. When processing spices, it is essential to use specialized equipment for tasks like cleaning, grinding, blending, and packaging.
Since they add flavor, color, and aroma to any dish, spices have long been a useful addition to the kitchen. Spices like coriander, cumin, and turmeric are now more than just ingredients; they are a thriving industry. Anyone working in the food industry or just curious about how spices are transformed from unprocessed farm produce to the fine powders we use in recipes needs to understand the processing of spices. Establishing a productive, economical, and superior spice processing facility can have a significant impact on those in the industry. Spice processing might be the solution if you want to improve the quality of your spices. This guide explains the steps involved in processing spices, how it operates, and the advantages it provides.
SOURCING THE BEST RAW MATERIALS
Careful raw material selection is the first step in the processing of spices. To guarantee a consistent and superior supply of spices, Indian spice manufacturing companies form solid alliances with regional farmers and growers. Selecting the right raw material is crucial because it establishes the flavor and aroma that will characterize the finished product.
Second, Cleaning and Sorting
The raw spices are carefully cleaned and sorted once they arrive at the manufacturing facility. In order to eliminate contaminants, foreign materials, and impurities that might have been introduced during cultivation and transportation,

Introduction

Spices are high value export oriented crops extensively used for flavouring food and beverages, medicines, cosmetics, perfumery etc. Spices constitute a significant and indispensable segment of culinary art and essentially add flavour, colour and taste to food preparations. Farm level processing operations are important for value addition and product diversification of spices. It is essential that various operations like washing, threshing, blanching, drying, cleaning, grading, storing and packaging ensure proper conservation of the basic qualities like aroma, flavour, pungency, colour etc. Each of these operations enhances the quality of the produce and the value of the spice.

India is the largest producer, consumer and exporter of spices in the world. India produces more than 65 spices in different varieties out of the 109 spices listed by International Standards Organisation (ISO). India produces around 5.8 million tons of spices annually (2012-13), of this about 10% of the total produce is exported to over 150 countries. The USA, Europe, Australia, Japan, the Middle East and Oceanic countries are the major importers of Indian spices. Estimated world trade in spices is 1.05 million tons valued at 2750 million US $, out of which India has a significant share of 48% in quantity and 43% in value.

8.1 Introduction

Spices are appetizers and hence, esse ntially used in the culinary preparations. They also possess antioxidant, antimicrobial and antibiotic properties. Further, they possess medicinal properties and hence, play an important role in human health.

According to International Organization for Standards (ISO), there is no clear cut division between ‘Spices’ & ‘Condiments’ and as such they have been clubbed together. The term ‘Spices’ & ‘condiments’ applies to “such natural plants or vegetable products or mixtures there of, in whole or ground form, which are used for imparting flavour, aroma and piquancy and seasoning of foods”. Spices are divided into different groups based on their plant components such as floral, fruits, berries, seeds, rhizomes, roots, leaves, kernel, aril, bark, bulbs etc.

About 70 spices are grown in different parts of the world and a major portion of these are grown in India. Hence, India is called “The home of Spices”. It is well known that spices played a significant role in ancient times, in the development of civilization – the life of the people and trade among the nations of East and West. Spices like ginger, pepper and turmeric have an intense and immediate impact on sensory perceptions and exhibit strong physiological effects as food and medicinal constituents. Some spices and herbs like cardamom, saffron and vanilla, which have less intense but more delicate sensory effects, are well known for their contribution to fragrance and usage as food flavouring agents. The history of spices and their use in food and medicine in ancient India have been reviewed earlier [1-3].

1. GENERAL INFORMATION

Asian continent, particularly India is known as “Land of Spices”.

Spice crops are largely grown in tropics between 20⁰N and 20⁰S.

Term ‘spice’ was derived from Latin ‘Species aromatacea’ means ‘Fruits of the earth’.

Spices and condiments are grouped under food adjuncts.

India is largest producer of spices and oleoresin.

Home of spices/ Spice bowl of India: Kerala.

Spices: It is defined as vegetable products (non-leafy parts) or mixture thereof, used for flavouring, seasoning and imparting aroma in food. It may be whole form or in ground form e.g. Pepper, Cardamom, Clove, Cinnamon, etc.