eChapters

1 What is the scientific name of Dill

 a) Acorus calamus b) Pimenta dioca

 c) Pimpinella anisum d) Anethum graveolens

 2 Important chemical content present in Asafoetida is

 a) Phenol b) Terbein

 c) Ferumine d) Limone

 3 Karimunda is a variety of

 a) Ginger b) Black Pepper

 c) Cardemom d) Cumin

Description of important spices and condiments

Introduction

Spices and aromatic herbs have been used since antiquity as preservatives, colorants, and flavour enhancers. Spices, which have long been the basis of traditional medicine in many countries, have also been the subject of study, particularly by the chemical, pharmaceutical, and food industries, because of their potential use for improving health. Both in-vitro and in-vivo studies have demonstrated how these substances act as antioxidants, digestive stimulants, and hypolipidemics and show antibacterial, anti-inflammatory, antiviral, and anti-carcenogenic activities. These beneficial physiological effects may also have possible preventative applications in a variety of pathologies. The chapter presents an overview of the potential of spices and aromatic herbs as functional foods ingredients.

Introduction

Spice – definition and uses

SPICES are aromatic or pungent vegetable substances, used as whole, broken, or ground form, whose function in food is seasoning rather than nutrition and commonly used as a condiment. It may be a dried seed, fruit, root, rhizome, bark or vegetative substance or flower bud. American Spice Trade Association (ASTA) defines spices as “any dried plant product used primarily for seasoning purposes”. Included are tropical aromatics (pepper, cinnamon, cloves, etc.), leafy herbs (basil, oregano, marjoram, etc.), spice seeds (sesame, poppy, mustard, etc.) and dehydrated vegetables (onions, garlic, etc.). Many spices have other uses like food preservation, medicine, religious rituals, cosmetics, and perfumery or as vegetables.

Introduction

According to FAO estimates, about 50% total geographical area in India is under various degraded hazards and about 2.1 million ha of land is getting degraded and deforested annually (Sehgal et al. 1998). Out of that underground water in arid and semi-arid areas is saline or sodic in nature. While spices, medicinal and aromatic plants may be a boon for these areas, which not only withstand under adverse conditions but also could be productive and helps in mitigating these problems. However, other crops are remain less or unproductive in these areas.

Spices are low volume and high value crops and have tremendous export potential, which is witnessed by several invasions faced by this Indian peninsula during ancient time. Medicinal and aromatic plants have unequally market potential with the world demand of herbal products growing at the rate of 7 percent per annum (Aishwath and Tarafdar, 2007). Seed spices and aromatic plants are the natural source of flavour, perfumes and fragrance widely exploited by essential oil industries in the world. Owing to considerably diversity of edaphoclimatic conditions ranging from temperate to tropical and arid to sub-humid that prevails in India and an array of medicinal and aromatic flora occurs in wild state. Many exotic essential oil bearing plants, including mints, citronella, geranium, patchouli, lavender and chamomile have been naturalized in India. As the country has emerged as one of the main producers of seed spices and some of the essential oils, theses plants are significant foreign exchange earners. Some of the aromatic plants especially grasses can sustain both biotic as well as abiotc stresses, even in the areas unsuitable for conventional crops (Aishwath et al., 2008). Most of the seed spices crops are seasonal crops and comes up well with limited moisture and nutrients. Therefore, underutilized natural resources can be utilized profitably by crop diversification.

Introduction

Spices hold a special place in world trade and were a catalyst in many historical events, discoveries, wars and inventions. It has even been cited as an important element in the spread of knowledge and culture. Spices are non-leafy parts of plants used as a flavoring or seasoning. Asia is known as ‘Land of Spices’ as it is the place of origin, production, consumption and export of most spices. Out of the world’s 70 plant species grown as spices, 13 are considered major spices produced in Asia. Spices had many uses, from adding flavour to food to medicinal properties, but only grew in a few places. In the last decade, the annual average growth in spice production was 4.3% and trade in spices grew at an average of 5.8% /year. More sophisticated consumers, health-conscious eating habits (but also the rise in the amount of processed foods that use herbs and spices as inputs), rising incomes, increased world trade and globalisation are driving this new era of profitability. Spices are ideal commodities for international trade. They are normally exported in raw or dried form and are usually easy to store and transport. Consequently they do not need expensive infrastructure such as cold chain management or advanced storage facilities and they are not particularly time sensitive. In addition, they are recognised as high value products that can be produced relatively easily on small farms and do not require investments in advanced technology and machinery.

Spider lily (Hymenocallis littoralis) is a perennial bulbous plant distinguished by its fragrant white flowers, central corona and spider-like appearance. It is an important commercial loose flower crop in western India, particularly Gujarat, Maharashtra and Karnataka, where flowers are used for garlands, Veni, Gajra and ceremonial decoration. The chapter describes its origin, distribution, genetics and botanical characteristics. It explains climate and soil requirements, bulb propagation, land preparation, planting, nutrient management, irrigation and cultural operations. Pest and disease management, harvesting and postharvest handling are also detailed. The chapter includes storage techniques for flower buds and discusses the long productive life and commercial yield potential of the crop.

Morphological characteristics

Nardostachys species is a perennial rooted herb with a strong woody main root that grows 10-60 cm tall. The rootstock is strong and lengthy, with petioles of withered leaves clinging to it. The stems are pubescent above and glabrate below. Leaves are both Cauline and radical. Cauline leaves are sessile, occur in two to three pairs, are 2.0 cm 0.6 cm in size, decrease in size from base to top, and are oblong or sub-ovate in shape. Radical leaves are large (15-22 cm 1.5-2.5 cm), six to eight in number, longitudinally nerved, slightly pubescent, and narrow down into the petiole, whereas cauline leaves are sessile, occur in two to three pairs, are longitudinally nerved, The jatamansi rhizome is dark brown, tapered, and densely coated with the remains of old leaf bases, giving it the appearance of a heavy beard, and hence the name jatamansi. The fibrous rhizome may be 6-9 cm long with about 20-30 cm-long yellowish taproot. A single root may bear 30-50 rhizomes.

Floral characteristics

Flowering shoots are 30–40 cm tall and produce 40–50 blooms in three to seven condensed cymes at the summit. Flowers are campanulate and range in colour from pinkish red to bluish white. The hairy corolla tube is 6 mm long. The 4-mm-long hairs on the fruit are capped by the sharp, often dentate, calyx teeth. Flowering takes place between June and August, depending on elevation, while fruits takes place between September and October. Seeds are gathered in October when they turn pale brown in colour.

Spinach is estimated to have originated in ancient Persia some 2,000 years ago, and was transported to India and ancient China as the “Persian vegetable” via Nepal in 647 AD. The Saracens introduced spinach to Sicily around 827. Spinach (Spinacia oleracea), also known as vilayati palak in Hindi, is a leafy vegetable that grows well in cool or temperate climates. Vilayati spinach is dioecious, unlike beet leaf spinach, which is hermaphrodite. Its production is restricted to household gardens in mountainous regions of India, and it is consumed as a cooked vegetable.

“Every year, 26,688,150 tonnes of spinach are produced over the world.” With a production output of24,484,507 tonnes per year, China is the world’s greatest spinach grower. The United States of America is in second place, producing 323,620 tonnes each year. “Spinach isn’t grown in India.”

Momordica dioica, also known as bristly balsam pear, prickly carolaho, teasle gourd, and kantola, is a flowering plant of the Cucurbitaceae/gourd family that is commonly known as spiny gourd or spine gourd. It is consumed as a vegetable throughout India and parts of South Asia. It is valuable commercially and is exported and utilised locally. The fruits are sometimes served with meat or fish and are prepared with spices or fried. Underground tubers are used to proliferate it. Small leaves, small yellow flowers, and small, dark green, spherical or oval fruits characterise this plant. It is dioecious, which means there are separate male and female organisms.

Spine gourd or teasle gourd (Momordica dioica : Roxb Syn.Momordica roxburghii G. Don, M. wallichi, M J Roem) is a rhizomatous, perennial and distinctly dioecious species of family Cucurbitaceae and  grows widely in many parts of India, mostly confined to  Eastern and South Eastern states. Vernacular names are kartoli,kakrol,meethakarela, padora, bhatt karela, palupagal, tumba, tumpai, golkandra, kankad, kankera, bara-karela, kankoda, kankodan, kantola and phalguni. In India it is mainly grown in Orissa, Bihar, and West Bengal .It is  found in nature in the Punjab, Uttar Pradesh, Rajasthan, Madhya Pradesh, Kerala and Maharashtra especially in areas with the least human interference. This is  a delicacy for its typical taste, attributed to high seed protein content. It also contains high amount of carotene (162 µg/100 g of edible portion) (Gopalan et al., 1982) and its tuberous roots have medicinal value too. It is a cheap source of vitamins and minerals (Bhuiya et al., 1977). This vegetable is gaining popularity  because of its high demand in  market. Certain limitations like lack of awareness among  farmers, non-availability of quality planting materials of standard varieties, unpredictable sex ratio in seedling progenies are arresting the expected spread of this nutritious vegetable (Ali et al., 1991).

The cultural history of India is associated with religious believes and any emphasis on arguments dealing with plant-man-God relationship appeals to people even today. This attitude prevails throughout India and many places of worship have continued to preserve in situ biodiversity of specific species of plants.

Leptospira 

Antony Van Leeuwenhoek (1681) first observed the Spirochaetes under the microscope and described them. In 1833, Ehrenberg used the name Spirochaeta for free living spiral shaped organisms. Obermeir (1873) first described a Spirochaete of pathogenic significance isolated from human patients of relapsing fever. Inada et al. (1916) first isolated Leptospira icterohaemorrhagiae from human patients of Weil’s disease. In 1980, Nogochi proposed the name Leptospira.

      In India, Taylor and Goyale (1931) first reported Leptospira from human patients suffering from jaundice in Andaman Islands (Srivastava, 2006). Ayyar (1932) first reported Leptospira from dogs in Chennai city. 

Properties of genus

1. Morphology : Leptospira are gram negative, spiral shaped organisms with hooked ends. Length varies from 6-20 µm with 0.1-0.2 μm diameter (Fig.1). They have a typical double membrane covering. The cytoplasmic membrane and peptidoglycan cell wall are closely associated and are surrounded by an outer membrane. Two periplasmic flagella (endoflagella) are found within the cell and are responsible for the motility. No flagella are present as extracellular appendages. One end of the flagella is attached with the pole of the bacterial cell by insertion disc. Other end extends towards the centre of the cell and may overlap the flagella coming from the opposite end. The endoflagella consists of a solid inner core surrounded by tubular sheaths. The FlaA and FlaB proteins constitute these flagellar sheath and core, respectively.

The order Spirochaetales contains two families, Leptospiraceae and Spirochaetaceae. It comprises spiral or helical bacteria (spirochaetes) which share some unique morphological and functional features. Members of the order are motile by means of endoflagella which are located within the periplasm.
The spirochactes are slender, motile, flexuous, unicellular, helically coiled bacteria ranging rrom 0.1 -3.0 μm in width. The outer sheath, the outermost layer of a spirochaete cell, is a multi layered membrane that completely surrounds the peri plasmic flagella (axial filament) and the helical protoplasmic cylinder. The cylinder consists or the nuclear material, cytoplasm, cytoplasmic membraneand the peptidoglycan portion of the cell wall.
The periplasmic flagella are wrapped around the cylinder and are in the peri plasmic space of these Gram-negative bacteria. One end of each flagellum is inserted near a pole of the protoplasmic cylinder and attached by platelike structures called insertion discs. The distal end of each flagellum is not inserted and extends to the centre of the cell and may overlap the flagellum from the opposite end. The periplasmic flagella facilitate the motility of the bacteria in viscid environments.
Leptospira species
Members of this species (leptospires) are motile helical bacteria (0.1 x 6 to 12 μm) with hook-shaped ends. Although cytochemically Gram-negative, they do not stain well with conventional bacteriological dyes and are usually visualized using dark-field microscopy. Silver impregnation and immunological staining techniques are used to demonstrate leptospires in tissues. Leptospirosis, which can affect all domestic animals and humans, ranges in severity from mild infections of the urinary or genital systems to serious systemic disease.

Causative Organism: Borrelia anserina

B. anserina and B. burgdorferi (borreliosis of humans) share common flagellar antigens, suggesting their close relationship. B. burgdorferi infect both mammals and birds (mallards and bobwhite quail). Geese, turkeys, chickens, ducks, pheasants and canaries are natural hosts of B. anserina. All age birds are susceptible.

Transmission

Infection is mainly transmitted by a soft tick- Argus persicus. Transmission is also by ingestion of blood, excreta or tissues from infected or dead birds, through contaminated feed or water. Use of syringe and needles on multiple birds also makes transmission possible.

This chapter focuses on split plot and strip plot designs, essential tools in experimental design known for effectively handling complex experiments. It covers the key principles and applications of these designs, emphasizing how they can be used to address specific research challenges. The chapter also provides practical guidance on conducting analyses of split and strip plot designs using R software, making it a valuable resource for researchers seeking to apply these methods in their work.

Split Plot Design

The split-plot design is a statistical approach widely used in experiments where specific conditions apply:

• Hierarchical arrangement of treatments: This design is ideal for experiments involving two levels of experimental units, typically “whole plots” and “subplots.” The main treatments are applied to the whole plots, while the sub-treatments are applied within these to the subplots.

• Adherence to experimental design principles: The split-plot design follows the principles of randomization, replication, and local control, but with a hierarchical twist. The main treatments are randomized across the whole plots, and the sub-treatments are randomized within these main plots. • Resource management in unequal experimental conditions: This design is particularly useful when some factors require larger experimental units or when changing levels of one factor is more resource-intensive than changing levels of another factor.

In factorial experiment it may sometime happen that certain factors require bigger plots than other for convenience of operation of the experiment.  For example, in an agricultural experiment involving two factors, say, irrigation and fertilizer, it is organizationally very inconvenient to apply different levels of irrigation to small neighbouring plots.  But there is no such difficulty for the application of different levels of fertilizer. To meet such situations it is desirable to have different sizes of the experimental units in the same experiment.