eChapters

Materials Required

Fruits/ vegetables, weighing balance, plastic crates, CFB boxes or other packaging material fruit pressure tester, hand refractometer, markers etc.

Procedure

    1.    Harvest the fruits
    2.    Subject them to various pre-treatments (as discussed in previous exercise).
    3.    Record their initial parameters i.e. weight, firmness, surface colour, TSS, rotting etc. 
    4.    Place properly packed commodity in storage alongwith a check under ambient conditions
    5.    Record the observations regarding changes in quality during storage
 

Gross evaluation is generally done to get a rough idea of the quality of semen and to assess the semen producing capacity of the bull. This also gives a fairly good idea about the reproductive health and any pathological conditions of the semen donor. The following are recorded under gross evaluation of semen:

    11.1    Color and appearance of semen
    11.2     Semen Volume
    11.3    pH of semen
    11.4     Semen consistency/ Semen density
    11.5     Presence of foreign matter
    11.6     Gross motility

A. Answer the following questions:

1.    What is Basis of scientific feeding?

Ans: Knowledge of the quantitative needs of the body for various nutrients and the relative value of feeds as sources of them is the basis of scientific feeding.

2.    What is the minimum requirement of animal per group to conduct a feeding trial?

Ans: Minimumanimal per group should be 4.

3.    When you will go for t test and ANOVA test?

Ans: In case of two rations, ‘t’ test and if it is 3 or more then ‘Analysis of Variance’ or ‘ANOVA’ test is applied.

4.    What are the advantages of feeding trials with laboratory animals?

Ans: It involves much less cost of animals, less feed and labour cost,requiredlesstime,individualvariabilitycanbe minimized due to similar genetic and nutritional history which is not possible in case of large animals, large number of animals can be used at a time and easy to slaughter the animals for histological and chemical analysis

5.    What is the purified form of nutrients?

Ans: Casein, urea or purified soybean protein for protein; starch, glucose or sucrose for carbohydrate; oils or lards as source of fat; chemically pure salts as minerals; pure crystalline compounds as vitamins.

I. FILL UPS

1. _____ is a legally sanctioned system for quality control on seed multiplication and production.
2. Certification consists of _____ phases.
3. In single application, the maximum area that can be offered for certification is _____.
4. AOSCA stands for_____.

2.1. Origin and distribution

• Native to India and Sri Lanka.

• Widely cultivated in tropics and subtropics.

2.2. Economic importance

• Cochin grass or Malabar grass or East Indian or True Lemon grass; perennial and multi cut aromatic grass.

• Lemon like odour due to presence of 70-90% citral, a cyclic monoterpene.

• Other compounds are geraniol, citronellal etc.

Key Points

• Seizures are common in dogs and cats but uncommon in larger animals.

• Animal seizures can be induced by several circumstances.

• Idiopathic epilepsy is the leading cause of seizures.

• Rapidly rising core body temperature and metabolic alterations lead to fatal outcomes.

Introduction

A seizure is defined as any rapid and uncontrollable movement of the body induced by aberrant neuronal activity in the brain. Seizures are common in dogs and cats, but uncommon in larger animals. Seizures can be severe, affecting the entire body, or moderate, affecting only a section of the body. The pet may or may not be conscious or responsive, and it may urinate.

Seizures are usually brief (1-2 minutes) and self-limiting; however, some animals may experience protracted activity (more than 5 minutes). Seizures that persist longer than 3 to 5 minutes might cause serious complications such as pulmonary or brain edema. Death is caused by a sudden rise in body temperature and metabolic alterations that reach a life-threatening threshold.

Antimicrobial Susceptibility Test
The routine use of the modified Kirby-Bauer method is recommended.
Material
• Sterile cotton swabs
• Soyabean – Casein digest broth or Tryptone soya broth
• Non selective agar such as nutrient agar.
• 0.5 Mc Farland turbidity standard:
Solution A
Bacl2, 2H2O. 1.75G
Make upto 100 ml with distilled water.
Solution B
H2SO4 1.0 ml
Make upto 100 ml with distilled water.
Stock Solution
0.5 ml of solution A + 99.5 ml of solution B. Dispense in 5 ml amounts.
Seal and store in dark.
• Glass vials or tubes 10 ml capacity
• Muller – Hinton agar
• Antimicrobial discs
• Sterile forceps
• Ruler or calipers

To understand how radiographs are produced by using X-rays, an understanding of how photons of diagnostic range can interact with matter is necessary in five possible mechanism of interaction:
    ·    Coherent scattering.
    ·    Photoelectric absorption.
    ·    Compton scattering.
    ·    Pair production.
    ·    Photodisintegration.

Materials Required

Fruit, pulper, fruit mill, hydraulic press, screw type juice extractor, stainless steel pans, chemical preservatives, glass bottles, closures, crown corks, crown corking machine, plastic barrels.

Procedure

    1.    Select and weigh sound fruits/vegetables for extraction of juice/pulp.    
    2.    Wash and peel the fruits as required.
    3.    Extract the juice by hot or cold process.
    4.    Pasteurize the juice/pulp to inactivate the enzymes and partially kill microorganisms.
    5.    Preserve the juice/pulp by heat processing after hot filling and hermetic sealing or by addition of permissible quantities of suitable preservatives.
    6.    Label the bottles properly and keep them for storage

If the 1990s were the decade of genomics, the first ten years of the new century are set to become the decade of proteomics. For the first time, the technologies of proteomics make it possible to generate quantitative protein expression data on a scale and sensitivity comparable to that achieved at the genetic level. This advance has major implications for our understanding of cellular organisation in health and disease and for pharmaceutical and agricultural biotechnology. Indeed, proteomics is already yielding important findings, across a wide range of applications. Most proteomics research, however, is directed towards the more proximal goal of investigating protein expression and function under specified physiological conditions.

The rapid evolution of proteomics has continued during the past years with a series of innovations in the core technologies of two-dimensional electrophoresis and mass spectrometry, and a diversity of productive research programmes. Well annotated proteomics databases are now emerging in a number of fields to provide a platform for systematic research, with particularly promising progress in clinical applications such as cardiology and oncology. Large-scale quantitative research, comparable in power and sensitivity to that achieved for gene expression, is thus becoming a reality at the protein level.

I. FILL UPS

1. _____ is the observation of number of plants / earheads of seed crop which should be observed during field inspection as one unit depending upon cropping pattern and crop.
2. Number of plants / earheads to be observed in one count for paddy is _____.
3. Number of plants / earheads to be observed in one count for sesame is _____.

Nowhere in the plant kingdom do ecology, evolution, and human affairs come together as intricately as phenomena involving secondary metabolites. The importance of these compounds is apparent in some of the earliest annals of human history. For example, Papaver somniferum, the original source of morphine and codeine, was known to the Sumerians in 4000 B.C. as hul gil (joy-plant). These two alkaloids, so important in human affairs for many years, probably evolved within the genus Papaver originally as protective compounds against herbivores. As a testament to the importance of secondary metabolites,

P. somniferum was one the first plants to be cultivated for a reason other than its caloric or nutritive value. Sadly, its importance has only increased over the time. Organic chemists of the nineteenth century were among the first to recognize and study the diversity of plant secondary metabolites and to analyze them both in terms of structure and function. The German organic chemists developed techniques to acetylate morphine and produce what they claimed at the time to be a non addictive version of the drug. However, acetylation product of morphine trade name Heroin, was vastly more addictive. The economies of several developing nations in Asia still depend on the acetylation of this plant secondary metabolite, and tens of thousands of lives each year are destroyed by its commercial importance. In another striking example of profitable secondary metabolites, acetylation techniques to salicylic acid, derived originally from the bark of willows, Salix, to create aspirin. The modern appreciation of these secondary metabolites from ecological and evolutionary perspectives had its spread outside of the plant-insect ecology community. The overwhelming array of chemicals found in plants was more than simply by-products of primary metabolism that served as waste products for these organisms that lacked excretory systems.

Ecologists and evolutionary biologists soon took up the cudgels, and the past 60 years have seen the development, testing, refinement, and, in some cases, rejection of models that describe the ecology and evolution of these “secondary” substances. With the functional roles of these compounds broadly acknowledged, several challenges presented themselves to scientists interested in their ecological and evolutionary dynamics. Most obviously, the details of the processes underlying the interactions between each of these compounds and their target pest or pathogen became one of the most vibrant topics in ecological and physiological research.

Captive breeding programme is generally considered as a process of breeding animals in controlled environments within well-defined settings, such as wildlife reserves, zoos and other commercial/noncommercialconservation facilities.Use of modern reproductive biotechnologies or assisted reproductive techniques (ART) include in general artificial insemination, embryo transfer/sexing, in vitro fertilization, gamete/embryo micromanipulation, semen sexing, genome resource banking, and somatic cell nuclear transfer (cloning) in conservation programs for endangered mammalian species.

Banks and Banking

On account of multifarious activities of modern banks, the Bank and Banking has been defned by several economists as follows:

The defnition of a banker given by Dr. L. Herber and L. Hart is “one who, in the ordinary course of business, honours cheques made upon him by persons from and for whom he receives money on current accounts.”

Chamber’s Twentieth century Dictionary defnes a bank as an, “institution for the keeping, lending and exchanging etc. of money”.

The purpose of a banker, in Crowther’s words, is to produce money by accepting other people’s loans in exchange for his own.

A bank, according to Professor Kent, is “an entity whose main activities entail the collection of the momentarily idle money of the general public for the purpose of advancing to others for consumption.”

Preventive Homeopathy

Homeopathy is entirely a unique philosophy different from that of conventional modern medicine. It is considered as a tailor made system of therapy administering a medicine fitting well in the totality of physical and psychological symptoms seen in an ailing individual whether it is human or animal. Homeopathy does not restrict to restoring health of ailing subjects (treatment of clinical diseases) but also offers scope for preventing diseases as well. It can play significant role in preventing enzootic and epizootic infectious diseases through administration of nosodes.

Nosodes

Nosodes are important class of homeopathic drugs specifically prepared from secretions or fluids of the diseased subjects or their infectious agents in the similar manner as other homeopathic drugs are made (process of dilution and succession). The word “nosode “ is derived from the Greek “nosos” (disease) and “eidos” (like). Nosodes are potentized remedies prepared from diseased tissues , discharges (products of disease) or disease causing agents (bacteria or virus) in a specialized manner following standard protocol. Dr. Samuel Hahnemann first prepared remedies from diseased tissues (mainly the miasmatic nosodes) for the three miasms (Psora, Sycosis and Syphilis) corresponding as Psorinum, Medorrhinum, and Syphylinum. Of which first two have common use in veterinary homeopathy. The nosodes have undergone proving similar to other drugs, and their use in veterinary homeopathy is in the same manner as with any other remedy i.e. matching patient symptoms with those of the remedy.

Goods and services are created through process of production. After production they have to be transferred or moved from the original producers to the final consumers. This involves the creation of market and the undertaking of number of activities such as buying, assembling, transportation warehousing, risk bearing, financing, selling etc. This constitutes the subject matter of marketing. Hence there is the need for the study of market.

What is market?

Market is derived from the Latin word “Marcatus” meaning trade, merchandise, ware, traffic or place of business. In the ordinary language, market is a place where buyers and sellers meet personally and make their purchases or sales. But in the language of economics, market need not be a place but it may be an area or region meant for potential exchange between a group of buyers and sellers interested in negotiating the terms of purchase and sale of goods and services and such negotiations may be done face to face at a certain place or may be done through other means of communication such as mail, phone, cable, television or through business middle men. Whatever may be the means of communication there will be free exchange or perfect communication between buyers and sellers so that a fairly uniform price prevails for a particular commodity over the whole area at any given time.

Gene silencing is the suppression of gene expression through nucleotide sequence-specific interactions that are mediated by RNA. Many manifestations of RNA silencing-RNA interference (RNAi) in animals, quelling in fungi and post-transcriptional gene silencing (PTGS) in plants, appear to be related. RNA silencing is experimentally activated by double-stranded RNA (ds RNA) and is used as a powerful technique for specific inhibition of gene expression in a variety of organisms. In a natural context, ds RNA may be produced from rearranged loci by transcription from converging promoters or by host-or viral-encoded RNA-dependent RNA polymerases (RdRP). A key component of RNA silencing is 21-23 nt RNA known as short interfering RNA (Si RNA). In Drosophila, the siRNA is derived from ds RNA by the action of an RNase-III like enzyme named DICER. The siRNA guides a multi-subunit endnuclease, referred to as the RNA-induced silencing complex (RISC) and it specifically degrades RNA that share sequence similarly with the ds RNA.

I. FILL UPS

1. The objectionable weed of paddy is _____.
2. The objectionable weed of sunflower is _____.
3. The objectionable weed of oat is _____.
4. The objectionable weed of napier grass _____