eChapters

INTRODUCTION

The chemical compounds needed for growth and metabolism in plants are termed as nutrients. Mineral nutrition of fruit plants is a vital soil management practice in an orchard. The nutrients are converted to cellular constituents or used for energy purposes through metabolic process. Broadly the essential plant nutrients may be divided into macro-nutrients and micro-nutrients. Macro nutrients are needed in plants in relatively higher amounts than micronutrients.
 
Macro nutrients: C,H, O, N, P, K, S, Ca, Mg. 
Micro nutrients: Fe, Mn, Cu, Zn, Mo, B, Cl, Ni.
 
Altogether 17 elements are considered essential for normal growth and production of a plant.While elements like cobalt, sodium and silicon have been established as essential elements for some plant species and not all. C-H-O matrix accounts for over 90% of the dry matter and is usually 20 to 50% of the fresh weight of the whole plant. Chloride is a most unusual nutrient as it is often present in plants in high concentrations yet its essential requirement is very much lower. Chlorine like Na and K plays an important role in osmoticum. The nutritional needs of fruit trees can be assessed through leaf and soil analysis, tree growth and cropping, and past experience.

INTRODUCTION

Water is one of the most important inputs essential for tree growth, development and fruit production. Fruit trees need it continuously during their life in optimum quantities. It profoundly influences photosynthesis, respiration, absorption, translocation and utilization of mineral nutrients, and cell division besides some other processes. Both its shortage and excess affect the growth and development of a plant directly and, consequently, its yield and quality. In India, however, rainfall is notoriously capricious, causing floods and droughts alternately. Its frequency distribution and amount are not in accordance with the needs of the particular fruit crops. Artificial water supplies through irrigation on one occasion, and the removal of excess water through drainage on another occasion, therefore, become imperative, if the fruit crops are to be raised successfully. Water management in India, thus, comprises irrigation or drainage or both, depending considerably on the environmental conditions, soil, crops and climate. Applying the proper amount of water at the right time is important for reducing costs and maximizing profits. It is essential, therefore, that there be a scientifically sound method for determining when to irrigate and how much water to apply for optimum crop response and efficient use of resources.

INTRODUCTION

Plant growth regulators (PGR) are bioactive molecules that modulates the physiological processes in plants and are effective at ultra-low concentrations. Minute alteration in the concentration and bioactive forms of PGRs’ throughout the plant development phases regulate the flowering, fruit set and development. The use of plant growth substances to control fruit set, size, shape, and maturation has become important in agriculture today because they have the ability to increase fruit size, colour, and shape, thereby increasing the marketability. By hastening or delaying maturation the grower can utilize peak demands, avoid unfavourable environmental conditions, and extend the market period. PGRs are applied in fruit crops at all developmental stages for various purposes as discussed below: 

INTRODUCTION
 
Growth and development are often used interchangeably, however, these describe separate events during the life cycle of a plant. To understand these precisely, let us take the example of multicellular organisms like annuals, monocarpic or flowering plants (angiosperms) including fruits, vegetables, flowers etc. which begins their life from a single cell fertilized egg, the zygote. The zygote develops into embryo through the cell division and differentiation. When we sow the seed in garden or pots, in a few days a young seedling emerge. With the passage of time, the seedling grow in size, the number of leaves increases, in perennial plants branching occurs for few years, and after putting forth the requisite vegetative growth plant begins flowering, produces fruits, seeds and finally the plant senesce at certain period. Generally three terms “growth”, differentiation” and “development” are used to describe the different events during the life cycle of a plant.

INTRODUCTION

The propagation of plants has been one of the fundamental occupations of mankind since beginning of civilization. Plant propagation involves the application of specific biological principles and concepts in the multiplication of plants for useful purposes. The present day plant propagation industry is large and complex and involves not only the group that multiplies the plants for sale and distribution, but also a large group of industries that provide services, sell the product, is involved in regulation, provides consultation carries on research or is involved in teaching. The key person with in this complex, however, is the plant propagator who possesses the knowledge and skill either to perform or to supervise the essential propagation tasks for specific plants.

INTRODUCTION

Plant propagation involves three main aspects: knowledge of mechanical manipulations and technical skills which constitutes the art of propagation; knowledge of plant growth and structures which constitutes science of propagation and knowledge of different kinds of plants and their methods of propagation. The basic objective of plant propagation is to increase the number of desirable types and to preserve the essential characteristics of the plant. Generally, sexual propagation or the seed propagation is used to raise the seedling rootstocks on which to graft or bud the desirable types. The clonal rootstocks are multiplied through vegetative means- layering, cutting etc. Various sexual and asexual methods such as use of seeds, apomicts, specialised plant parts, cuttings, layers, grafts and buds are employed to multiply plants of different fruit crops. In this chapter, the techniques used for raising rootstocks and multiplying the desirable types of different fruits have been discussed.

INTRODUCTION

Plant tissue culture is basically in vitro culturing of any type of explants such as leaf, root, bud and stem or even single cell except the seed or embryo on a suitable culture medium under controlled environmental conditions. Plant tissue culture employs the totipotency of plant cells to produce whole plant. The term totipotency was coined by German plant physiologist, Haberlandt (1902) who is known as father of tissue culture techniques.

Definition of Micropropagation

Micropropagation is one of the best and most successful examples of commercial application of tissue culture techniques. The concept of micropropagation was first presented to the scientific community in 1960 by Morel, who produced the virus-free cymbidium through meristem culture. It entails in vitro propagation of plants starting with very small plant parts (0.2-10mm) grown aseptically, followed by their establishment in soil under green house conditions. It is the art and science of plant multiplication in vitro. This technique has been standardized and commercialized for various fruits and vegetable crops.

SEED GERMINATION

Germination is the growth of a plant contained within a seed; it results in the formation of the seedling, it is also the process of reactivation of metabolic machinery of the seed resulting in the emergence of radicle and plumule. The seed of a vascular plant is a small package produced in a fruit or cone after the union of male and female reproductive cells. All fully developed seeds contain an embryo and, in most plant species some store of food reserves, wrapped in a seed coat. Some plants produce varying numbers of seeds that lack embryos; these are called empty seeds and never germinate. Dormant seeds are ripe seeds that do not germinate because they are subject to external environmental conditions that prevent the initiation of metabolic processes and cell growth. Under proper conditions, the seed begins to germinate and the embryonic tissues resume growth, developing towards a seedling.

INTRODUCTION

For survival of young seedlings and rooting of cuttings it is essential that they maintain a healthy water and air balance especially to make up the water lost in transpiration. Water stress within the cuttings hinders metabolic activities and cell regeneration and consequently initiation of roots. So medium should be such that it provides adequate water required for rooting and growth. Also these substrates must provide initial nutrients necessary for plant growth. These should also contain micro-organisms that seedlings may need.

INTRODUCTION

The initial care of young seedlings and propagated plants is very necessary and important. Efforts should be made in such a way that the young plants can be propagated and grown to a saleable size with no insect-pests and diseases attack and nutrient deficiency. The supply of healthy plant material to the growers helps in good reputation of a nursery as well as to make fruit crops more profitable.

Sometimes young nursery plants suffers from various problems like attack of insect-pests and diseases, nutrient deficiency, scanty or excessive moisture and stagnation of excess or rain water in the field. The young seedlings/plants require light and frequent irrigation and need special care to make them grow well and to save them from adverse weather conditions. They must be protected from intense heat and cold. The young nursery plants growing on light or sandy soils can exhibit deficiency symptoms of macro and micro elements. Such nutrient deficiencies can easily be corrected by foliar or soil application of desired nutrient.

INTRODUCTION

With the change in world scenario from plentiful resources to limited with population increasing geometrically, shrinking land and climate change, the pressure on agriculture is mounting continuously. Meeting the nutritional requirements of population by optimum supply of food is the pre-requisite for sustainability of life under such conditions. Rapid urbanization is causing scarcity of cultivable land. Therefore, the productivity per unit area of the crops needs to be increased manifold. Horticulture, in general, has attained a pride status in the field of modern agriculture. However, radical changes are expected in horticultural practices in near future as the situation in terms of area as well as production is not as comfortable as the food grain production.

INTRODUCTION

Fruits of commercial importance comprise various combinations of tissues that may include an expanded ovary, the seed and other plant parts such as the receptacle (apple, strawberry), bract and peduncle (pineapple). They may develop from a single ovary of a flower as in simple fruits (apple, banana, mango, peach, plum, tomato etc.), number of ovaries of a single flower as in aggregate fruits (strawberry) or from a number of ovaries of different flowers as in compound fruits (pineapple). They are derived from members of a wide range of families of both monocotyledonous and dicotyledonous plants. Inspite the huge diversity in origin, structure, composition and climate adaptabilities that exists among different fruits, there is considerable similarity in the pattern of maturation and ripening that occur in different fruits.

Grading is defined as sorting of fruits into different grades according to the size, shape, colour, and volume to fetch high price in market. Grading of fruits after harvesting is an essential step in post-harvest management. Grading of fruits is done on the basis of physical characteristics like weight, size, colour, shape, specific gravity and freedom from diseases. The known methods of grading of fruits and vegetables are manual grading and size grading. Sizing produce is usually a part of grading and is optional for direct marketers, but may be worthwhile if certain size grades receive a higher price than others. Round produce units can be easily graded by sizing rings. Several types of mechanical sizers are available for small-scale operations. Sorting by maturity can maximize the market value of crops such as stone fruits and mangoes .The grades of different fruits suggested by Directorate of Marketing and Inspection (DMI) are given in Table 1.

In post-harvest management of horticultural crops, post-harvest treatments play an important role in maintaining the quality and enhancing the shelf life. There are number of treatments viz non-chemical, chemical and biological treatments which are being used commercially depending on the requirement of trade. Fresh fruits are living tissues subject to continuous change after harvest. Some changes are desirable from consumer point of view but most are not. Post-harvest changes in fresh fruit cannot be stopped, but these can be slowed down within certain limits to enhance the shelf life of fruits
 
1. Low Temperature Treatment

Pre-cooling: Pre-cooling refers to the rapid removal of field heat from freshly harvested fruits in order to slow ripening and reduce the deterioration prior to storage and transportation. The major effect of pre-cooling consists in reducing respiration rate, retarding the growth of decay organisms and reduces wilting and shrivelling, since transpiration and evaporation occur more slowly at low temperature. The harvesting of many perishable crops is done at evening or early morning to avoid excessive field heat.

INTRODUCTION
 
Once a crop is harvested, it is almost impossible to improve its quality. Therefore, proper storage conditions—temperature and humidity—are needed to lengthen storage life and maintain quality once the crop has been cooled to the optimum storage temperature. In modern times, the storage of perishables has become more sophisticated because of the demand for a wide range of fresh fruits and vegetables at all the times of the year and because of need to spread peaks of production over longer periods to maximize profit and reduce waste. But in our conditions, storage of fruits and vegetables is practiced to avoid the low prices during the glut season in anticipation that prices will increase during the lean period or off-season. Certain crops like potatoes and apples can be stored for long period whereas for the crops peach and plum, the storage period is very short. However, for both long-term as well as short-term storage, high level of technical know-how about the crop is necessary for successful storage. There are a few important factors, which need to be taken into account before storage of the produce.

INTRODUCTION

As per National Horticulture Database published by National Horticulture Board, during 2014-15 India produced 89.15 million metric tonnes of fruits. This value however differs from the consumption value depending upon the post harvest losses, storage losses or transportation losses. Transportation and distribution of fruits being the most important factor, plays a key role in determining the net income from horticulture trade. It is an essential link in the modern food chain and exists as complex matrix where not only fresh goods finished products are transported but there is also transportation of bulk raw materials. Locally grown produce may be transported directly from farms to retail outlet. For local market, the produce is brought by bullock cart, tractor, trollies. Mainly the long distance transportation is by trucks, rails which is costly. In India the transportation by Railway is much less than the other means. The road transport is 3-4 times more expensive than rail, yet it is preferred for shipment of fresh fruits simply because of the faster movement of perishable commodities.

1.1. INTRODUCTION

The word research derives from the French “recherche”, which means to search closely. Research is a process of investigation. It is an examination of a subject from different points of view. It’s not just a trip to the library to pick up a stack of materials, or picking the first five hits from a computer search. Research is a hunt for the truth. It is getting to know a subject by reading up on it, playing with the ideas, choosing the areas that interest you and following up on them. Research is the way you educate yourself.

Research can be defined as the search for knowledge or any systematic investigation to establish facts. The primary purpose for research is discovering, interpreting, and the development of methods and systems for the advancement of human knowledge on a wide variety of scientific matters of our world and the universe. Research can use the scientific method, but always not necessary.

Scientific research relies on the application of the scientific method, a harnessing of curiosity. This research provides scientific information and theories for the explanation of the nature and the properties of the world around us. It makes practical applications possible. Scientific research can be subdivided into different classifications according to their academic and application disciplines.

2.1. INTRODUCTION

The purpose of collecting data is to collect evidence for reaching a sound and clear solution to a problem. In this chapter we will study the sources of data, mode of data collection and its techniques.

Sources and Types of Data

Statistical data can be obtained from two sources:

Primary and

Secondary

3.1. CENSUS AND SAMPLE SURVEYS

Census or Complete Enumeration

A survey, which includes every element of the population, is known as Census or the Method of Complete Enumeration. If certain agencies are interested in studying the total population of a country, they have to obtain information from all the households in rural and urban area.

The essential feature of this method is that this covers every individual unit in the entire population. We cannot select some and leave out others. We may be familiar with the Census of a country, which is carried out after a particular period like every ten years. A housetohouse enquiry is carried out, covering all households. Human demographic data on birth and death rates, literacy, workforce, life expectancy, size and composition of population, etc. are collected and published by a government agency.

4.1. INTRODUCTION

The number of individuals to be included in a research study (the sample size of the study), is an important consideration in the design of many studies. This chapter will provide information on basic factors that determine an appropriate sample size and provides methods for its calculation in some simple, yet common, cases. Sample size is closely tied to statistical power, which is the ability of a study to enable detection of a statistically significant difference. A trade-off exists between a feasible sample size and adequate statistical power. Strategies for reducing the necessary sample size while maintaining a reasonable power is also important.

How many individuals will I need to study? This question is commonly asked by the clinical, ecological and behavioural investigator and exposes one of many issues that are best settled before actually carrying out a study. Consultation with a statistician is worthwhile in addressing many issues of study design, but a statistician is not always readily available.

The amount of information that can be gained from a sample depends upon its absolute size and not upon its size as a proportion of the population size, Just as to decide if the wine is good we need only a sip, irrespective of the bottle size, similarly we do not need to know the exact size of the parent population, provided complete homogeneity exists, But in reality, and more so in biological sciences, such homogeneity is rarely seen. Therefore, appropriate sample size becomes pertinent for drawing valid conclusions and is among the most difficult decisions to be taken for a study.

The size of the sample would depend upon (i) the permissible error in assessment of inference i.e. accuracy and (ii) sampling error. Sampling error measures the amount of variability between sample results; the lesser it is the better. The main determinant of the sample size, therefore, is how accurate results do we used. This in turn, depends on the purpose and type of study.

5.1. INTRODUCTION

The word ‘probability’ means “reasonable likelihood of happening of an event”. In biology it is difficult to predict an outcome with absolute certainty. Probability helps us to estimate the occurrence of relative frequency of any (biological) event and is an accepted measure of uncertainty.

Mathematically, the probability ‘P’ that an event ‘E’ will occur, is expressed as:

6.1. INTRODUCTION

The word hypothesis is made up of two words Hypo + thesis = Hypothesis. ‘Hypo’ means tentative or subject to the verification and ‘Thesis’ means statement about solution of a problem.

The world meaning of the term hypothesis is a tentative statement about the solution of the problem. Hypothesis offers a solution of the problem that is to be verified empirically and based on some rationale. Hypothesis is the composition of some variables which have some specific position or role of the variables i.e. to be verified empirically. It is a proposition about the factual and conceptual elements. Hypothesis is called a leap into the dark. It is a brilliant guess about the solution of a problem. A tentative generalization or theory formulated about the character of phenomena under observation are called hypothesis. It is a statement temporarily accepted as true in the light of what is known at the time about the phenomena. It is the basis for planning and action in the research for new truth.

1.1. INTRODUCTION

Researchers interested in taking up research have initial difficultes in picking up a problem worth for investigation. At time they waste considérable amount of time in just thinking about the pros and cons of problems suggested by some ones or identified by himself/herself. It is basically due to misunderstanding about "what is research". Usual notion about research is that it must resuit in something spectacular or it must come up with a new discovery or theory. It must be pointed out that a study which brings forward new facts, or which demolishes or establishes a hypothesis or a theory or principal already in vogue, or which puts a variety of information in to order or system, or which prepares ground for further research is as valid as one which ends with a new discovery. The best research problem for researcher is one which arises from the researcher's own curiosity. The only thing is that researcher must know the tools which can handle the problems he proposed to take up. Before choosing for a problem for research one should as question to himself: is this problem really of interest to me?; Is it significant in the sense that it extends the frontiers of knowledge or fills an important gap in knowledge?; Can I handle this problem within the constraints of resources of money, time etc?; has this problem not been investigated by someone else? A good research proposai describes situation of problem, problem area, justification, methodology adopted for data collection, analysis, expected results and institution/ organisation that may use the outcome.

8.1. INTRODUCTION

The existence of Science could be in danger, if scientists did not record experiment performed, data collected and resuit obtained. Scientific writing doesn't mean to only keep records of progress and information, but to actually publish the results of studies in scientific journals. While keeping records is quite easy to do, publishing scientific papers can be really difficult, and especially for young researchers who are just starting to discover the world of scientific publications. A scientific writing is a method of communication, an attempt to tell others about some specific data that you have gathered and what you think those data mean in the context of your research. The rules of writing a scientific paper are rigid and are différent Uorr\ those that apply when we write an English theme. Scientific paper must be written clearly and concisely so that readers with backgrounds similar to yours can understand easily what you have done and how you have done it. The ultimate aim of most scientists is to publish their research findings in professional scientific reports. This includes peer-reviewed journal articles, which report original findings and contribute to the global pool of scientific knowledge. Scientists spend their time writing proposais, planning research, undertaking experiments, analysing data, tracing research, and reading related articles. Published reports and journal articles provide evidence of this dedication. Therefore, a scientific report is not simply some extra work that is done at the end of a project; it is an important historical document that provides evidence of work to the wider scientific community.

9.1. INTRODUCTION

A data are generally voluminous, so they need to be put in a compact and presentable form. This chapter deals with presentation of data precisely so that the voluminous data collected could be made usable readily and are easily comprehended. There are generally three forms of presentation of data:

Textual or Descriptive presentation

Tabular presentation

Diagrammatic presentation

Estrous cycle

After puberty the female enters a period of reproductive cyclicity, which continues throughout most of her productive life.  Estrous cycle is the rhythmic sexual behavioral pattern exhibited by the female, which begins at one estrus (heat) and ends at subsequent estrus.   It is the period between two estruses of the regular cycle.  The reproductive activity of the female is maintained by the co- ordinated functioning of different organs of reproduction (Table: 1.1). Estrous cycle provides repeated opportunities for the females to become pregnant. When conception does occur, the female enters a period of anestrous during pregnancy, which ends after parturition (giving birth) and uterine involution (repair and returning to normal size).

In our country livestock are mostly dependent upon cereal by-products or the crop residues such as straws, stovers etc., which are low in nutritive value. According to the National Commission on Agriculture (1989-90), there is a shortage of 40% of concentrates, 20% of dry fodder and 40% of green fodder for meeting the nutritional requirements of livestock. Consequently the livestock is surviving on low weight, low energy and thereby leading to problems in the production and reproduction. Nutritional deficiencies as well as imbalances are implicated as the cause of infertility in animals. The clinical signs observed with the deficiency of a specific nutrient as variable depending on the degree of the deficiency. The information on the important relationship between nutrition and health is necessary for recognizing and correlating the reproductive problems.

Livestock diet or ration may be made up of one or several ingredients depending on the species of animal, their physiological state, source of feed and its nutritional composition, nutritional requirements of the animals and voluntary intake of the feed. Chemical and nutrient composition with each ingredient varies tremendously and no single feed ingredient is complete in its nutrient composition with respect to animal’s requirement.  Therefore, animal feed has to be balanced properly to supply required nutrients in appropriate quantity.  Feed formulation is an art of quantitative application of science of nutrition for the optimum supply of all the essential nutrients through actual feeding of animals. This exercise may be as simple as mixing of few ingredients with simple mathematical calculation or preparation of least cost balanced ration by computer programme.  Since the expenditure on feeding is substantial in livestock production, feed formulation needs special attention of nutritionists or feed technologists. The instruments and equipments used for feed formulation and estimation of micro minerals and ingredients like Feed grinder and mixer of capacity 75 kg, Fibertech for fiber estimation, Hammer mill-particle size reduction, Mixer and grinder for feed and fodder (complete feed preparation) of capacity 250 kg, Roughage processing-Chaff cutter, Soxhtech for fat estimation, UV-vis-Spectrophotometer and Voltameter for micro mineral estimation are shown in Fig. 3.1 to 3.8.

India has a mega animal bio-diversity, which is manifested in the form of availability of a large number of species, and a variety of breeds even within species. India is bestowed with rich and varied domestic animal biodiversity having a total of 32 breeds of cattle and 10 buffalo breeds. The diversity of livestock is not only accidental, nor purely natural; it is more the outcome of thousands of years of continuous selection. The trends in livestock population over the last three decades have shown that, there is significant increase in the population of buffaloes i.e. from 60.5 crore in 1973 to 96.9 crore in 2003, while cattle population has increased from 180.1 crore to 226.1 crore in the same period. The share of buffaloes, in the bovine population increased from 22 to 32 per cent while that of cattle has shown declining trend. Within the cattle, there has been marked shift from work animals towards milch animals which has resulted in loss of important draught breeds of cattle. Further indiscriminate crossbreeding has resulted in significant decline in number of important milch breeds like Sahiwal.

Faulty estrus detection is the most important problem in reproductive management.  There are two requirements for good estrus detection: a skilled observer must spend sufficient time to make observations and cows must show overt signs of estrus during the period of observation.  There are various ways to detect the estrus signs in cows and buffaloes as detailed below.

1.    Palpation of reproductive organs:  Routine rectal examination of all cows between 30-40 days after calving and individual cow that has not been inseminated within two months after calving should be encouraged.   This is to confirm that the reproductive tract is normal and to predict when the next estrus will occur or to identify cows for prostaglandin treatment when estrous cycles are occurring, but estrus has not been detected. 

2.     Teaser bulls:  Teaser bulls are useful in identifying estrus in large number of animals especially buffalo cows.   Teaser bulls can be prepared by vasectomy or by applying apron to a bull. 

3.     Heat detector animals:  Sexually active animals like bulls, cystic cows, hormone treated steer, cows and heifers can be used to identify estrus cows.    They may be fitted with halters containing ink- filled reservoirs and ball paint pen type devices (chin ball markers) that will mark animals that mounted or they can be used without these devices to increase the sexually activity and make visual detection programs more effective.

After the Second World War, the 1940’s and 1950’s was the wide spread establishment of artificial insemination and the introduction of frozen semen in cattle breeding.  In the late 1960s came revolution in measurement of hormones in the body fluid with the introduction of sensitive radio-immuno-assay (RIA) techniques, which provided a new approach to early pregnancy diagnosis, emerging around the same time were early forms of the ultrasonic which could also employed in the detection of pregnancy. The 1970’s were to see embryo transfer and freezing of embryos in cattle becoming commercial realities and their incorporation into increasingly effective breeding improvement programme with the availability of prostaglandins (PGs) and Gonadotrophin releasing hormones (GnRH) that decade also witnessed new possibilities for estrum control in cattle and horses.  Few years later came the ability to produce the cattle embryos in the laboratories in large numbers and at low cost.

Synchronization of estrus is making a group of cows to come into estrus at the same time.  Effective estrus detection requires much time, labour, skill and expense and it is a major limiting factor in artificial insemination programme in cattle.  It was the principal stimulus that led to research in the development of progestational and prostaglandin compounds. 

Advantages

1.     Improve the efficiency of heat detection

2.     Management tool, better planning of breeding activities and wider use of artificial insemination 

3.     Solve problem of unobserved estrus

4.     Maintain short calving interval

5.     Overall improvement in herd productivity and reproductive efficiency

6.     Controlled lactation

7.     Management of replacement heifers

8.     Useful in embryo transfer programmes

9.     Reduce labour

10.     Possibly reduce involuntary culling for reproductive reasons

Buffaloes, though have great production potential, do suffer with many reproductive problems viz.  delayed age of puberty and  sexual maturity, weak / silent estrus, longer inter-calving period,  seasonality in breeding, poor libido  and poor semen quality. Little information is available about the buffaloes as compared to cattle because all the time we try to apply the knowledge available about the cattle to the buffalo.  Some comparative reproductive parameters in cow & she buffalo & cow bull and buffalo bull are depicted in Table 8. 1 and Table 8.2 respectively.

The ultrasonography is a technique which uses sound waves of very high frequency to scan the various organs of the body. The ultrasound waves of 2-10 mHz frequency are used in veterinary practice. A quartz crystal when stimulated by short pulse current ultrasound waves are emitted. The ultrasound waves emitted are directed towards a specific target organ are reflected back as echoes. The echoes are received by the same quartz crystal and are processed in the electronic circuit and finally displayed as image on the monitor.

Laparoscopy is an operative procedure performed through a key-hole opening with a rigid endoscope that allows visual inspection of organs.  The laparoscopy helps in arriving diagnosis of various reproductive and digestive disorders quickly.  By laparoscopy clinician can visualize the external surface of the abdominal organs.  Tumours, abscess, cyst, lesions, growth, hemorrhage, adhesions of the organs in the abdominal cavity can be readily diagnosed by this technique.  Aspiration of follicular fluid, biopsy of lesions and sterilization are commonly employed maneuvers by this technique.  It is having both diagnostic and therapeutic value.  The procedure consists of placement of transabdominal rigid endoscope after establishment of pneumoperitoneum in the body cavity.   The images of different organs are displayed on the TV monitor when the laparoscope is moved within the peritoneal cavity with the illumination through halogen light source.

Non-specific uterine infections reduce the reproductive efficiency of livestock.  They are called nonspecific because the initial colonizing bacterium is not known and the specific bacteria causing the signs of infection are not known.  Even though numerous bacteria, in a variety of combinations, have been isolated from infected uteri, Corynebacterium pyogenes and Escherichia coli are usually associated with uterine infections in cattle and sheep.  Empirical therapeutic approach, prescriptions without scientific validation (extra-label use) or inadequate clinical judgment itself to ascertain the nature of uterine infection, obviously due to lack of diagnostic tools are the prime reasons for therapeutic failure for the unsuccessful treatment of uterine infection by large animal practitioners.

Buffalo plays an important role in Indian livestock economics.  India has more than the half of the world buffalo population, contributing 50% of total milk production of the country.  The buffalo is said more productive than an average cow due to the higher fat percentage (7%) in the milk, better feed conversion ability and disease resistance.  Buffaloes have lower reproductive efficiency in the form of delayed age of maturity, longer calving interval, weak / silent estrus, seasonality of breeding, poor libido and poor semen quality. A large number of buffaloes do not conceive due to some gynaecological problems and are being slaughtered every year.  The most commonly observed reproductive disorders are detailed below.

Infertility means reduced fertility or the cow is ultimately capable of becoming pregnant and giving birth to a live calf but the interval may be much longer than 12 months. 

I. Problems leading to infertility

1. No signs of estrus

When the animal is pregnant and for a short time after calving the cows may not show estrus signs. A thorough palpation of the ovaries and tubular genital tract should be performed and a repeat examination may be necessary.

A typical repeat breeder cow or buffalo is an apparently normal cycling cow / buffalo with normal genitalia which has failed to settle to services or inseminations during three or more consecutive estrus periods. These animals can neither be considered as sterile nor can settle to subsequent services.  Age of the repeat breeder should not have exceeded beyond 12 years.

The causative factors for repeat breeder animals fall in two main categories as failure of fertilization and early embryonic death.

Production is based on reproduction and health of reproductive system. Economy of livestock industry is directly attached to the maintenance of high fertility in the herd so controlling the reproductive diseases in cattle is a must.  Reproductive health may be disturbed by reproductive infections like Brucellosis, Leptospirosis, Infectious bovine rhinotracheitis and Bovine viral diarrhea.

There is no rapid direct test to determine fertility of semen sample. During freezing and thawing of semen 20-80% of the spermatozoa become immotile or die. In addition to this, there may be damage to spermatozoa by disintegration of sperm cell membrane and acrosome leading to loss of intracellular enzymes.   The only reliable method to evaluate the semen is to inseminate a cow and get a calf at the end of gestation period.  Now-a-days new methods have been developed for in-vitro assessment of fertility of spermatozoa.  Some of these methods are exhaustive, time consuming, need for a full fledged analysis and difficult under field conditions.

Several cryogenic agents have been used in many countries for freezing and preservation of bovine semen like solid carbon-di-oxide (-79°C), liquid air (-183°C), liquid helium (-268°C) and liquid nitrogen (-196°C).  Liquid nitrogen is 4th coldest substance known and its small amount is converted into a very large amount (near about 700 times) of its volume when exposed to atmosphere.  It is a colourless, odourless, non-irritant, non-toxic, non-caustic, non-corrosive, non-inflammatory and inert gas having boiling point -196°C temperature.  Being a liquid it comes into good contact with the surface of the semen straws and constant storage temperature is maintained throughout the straw and due to these properties LN2 is most widely used refrigerant in the world.

The various steps involved in frozen semen technology are vital as the quality of semen determines the conception rate.  The key factor enabling the successful long term storage of frozen spermatozoa is maintenance at ultra low temperature.  Effective semen storage depends heavily on good identification and stock control.  Frozen semen can be easily damaged in the process of repeated handling to establish identity.

Reproductive process involves many events that have to occur in a well balanced manner if satisfactory fertility rate is to be achieved. Any adverse  effect of  physiological, morphological  or environmental effect on the male  or  female may result  in failure  of  fertility  or reduced fertility  depending  on the severity of the cause. Apart from this, the role of person who stands between the cow and the bull play an important role. The efficiency of the person handling the semen and depositing in the reproductive tract of domestic animal will influence to a great extent on the conception rate in A.I. programme. It is observed that certain bovine population fails to conceive at a single service or series of services.   Some times there is loss of conceptus during gestation or produces a dead calf or weak calf that will die soon after birth.
 
Consequences of these factors affect cows and may have long calving interval, abortion or sterility, all of which tend to decrease the average daily production of milk.

The choice of hormones and drugs acting on reproductive system are important as therapeutic agents during reproductive disorders like anoestrus, metritis, pyometra, repeat breeder, retention of fetal membranes, delayed puberty, ovarian disorders etc.  The administration of specific chemotherapeutic agent is of paramount importance in treating reproductive disorders in dairy animals.  The dosage, route, frequency and indications of chemotherapeutic agents acting on reproductive system are mentioned in tabular form as below.

INTRODUCTION

Mango (Mangifera indica L.) belonging to Family Anacardiaceae is the most important commercially grown evergreen tree of tropical and subtropical regions of the world. Due to its wide range of adaptability, high nutritive value, colorful fruits, richness in variety, delicious juicy taste and excellent flavor, it possesses high commercial value. It is sometimes also called ‘apple of the tropics’. It is believed that cultivation of mango originally started in South East Asia where it is being cultivated for last four to six thousand years. Now, it is cultivated in 112 countries of the world. India has the richest collection of mango cultivars.

INTRODUCTION

Banana (Musa species) also known as ‘Apple of Paradise’ is an important and commercial fruit crop of tropical regions of the world. It is the only tropical fruit traded to the extent, that it is the 5th largest agricultural commodity after cereals, sugar, coffee and cocoa in world market.

The word ‘banana’ is now applied to all table varieties. Formerly, it was applied to ‘Nendran’ banana of Kerala which was however called ‘Plantain’ by Westerners. The English used the word ‘Plantain’ to represent cooking banana while in parts of South India, even sweet fruited clones were called ‘Plantains’. The variant with persistent bracts and male flowers with large fruits held in lax manner and eaten after cooking were grouped under plantains. All other types which had small fruits, held compact in a bunch with softening of the pulp when ripe, dropping of withered bracts and staminate flowers as the banana. In other words plantain are starchy, not sweet and cooked as vegetable. They usually resembles green bananas, are longer and have thick skin. Bananas are sweet, shorter and have thinner skin. They are eaten as a fruit and have yellow colour when ripe.

INTRODUCTION

Guava (Psidium guajava L.) is an important fruit crop of India. It is a tropical fruit but also grows well under sub-tropical conditions. Guava is one of the commonest fruits liked by all the people and is known as ‘Apple of Tropics’. It is one of the major fruit of India and considered the fifth most important fruit in area and production after mango, citrus, banana and apple. Guava is quite hardly, prolific bearer and highly remunerative fruit which needs relatively less care as compared to other fruits. It excels most of other fruit crops in productivity and food value and as such has wide adaptability to soil and climatic conditions. Guava is such a fruit which is practically grown all over the country on commercial scale and especially in kitchen gardens and near the tubewell premises. The best quality of guava in India is grown in the area around Allahabad. Guava bear heavy crop every year and offer handsome economic return to growers.

INTRODUCTION

Papaya (Carica papaya L.) is known as wonder fruit of the tropics. It is unique plant, which produces fruits throughout the year. It owes its popularity due to various reasons, i.e., requires less area per tree, comes to bearing in a year, easy to cultivate and has high nutritive and medicinal value. Papaya is an ideal fruit for growing in kitchen gardens, backyard of homes as well as in orchards, especially those near the cities or big towns. India ranks first in production of Papaya in the world with share of 31% of the total production. Its productivity is highest (43.70 t/ha) among the fruits grown in. It is also  India. It is famous intercrop due to its small stature, short life cycle, precocious in bearing and grown extensively as a filler crop in orchards.
 
Papaya is quick growing, bearing fruits within 8-10 months of transplanting and giving yield in same year. Fast growth with high photosynthetic rate, reproductive precocity, low production cost and more output characterize the uniqueness of this fruit. Now a day, the area under this crop is growing rapidly due to its higher productivity. Indeed, papaya cultivation is very profitable if done on modern lines. Demand for its fruits is growing in domestic as well as export markets and require modern technological interventions for commercial exploitation of these fruits. A ready market for its fruits becomes a big bonus for growers. Immediate availability of a selling point and a good price from a buyer encourage the farmers to grow more papaya crop.

INTRODUCTION

Pineapple [(Ananas comosus (L.) Merr., Bromeliaceae)] is a commercial, tropical American crop, popularly known as “Golden Queen” all over the globe. The name ‘pineapple’ was given in the mid-17th century because of its resemblance to ‘pine cone’. Its botanical name Ananas comes from ‘anana’, the Tupi word for the fruit, meaning “excellent fruit” and comosus means tufted and refers to the stem of the fruit.
 
Pineapple is a monocotyledonous, low-growing,herbaceous and perennial with a spiral of spiny sword-shaped leaves on a thick stem. It has 90 to 100 cm height with spreading leaves, which give the plant a rosette appearance.The plant bears a single fruit terminally on a peduncle protruding out from the centre of the rosette. The fruit is mostly a large juicy fruit containing aromatic, edible, yellow flesh surrounded by a tough segmented skin and topped with a tuft of stiff leaves.