eChapters

Materials Required

Soxhlet apparatus, petroleum ether, water bath, sample, grinder, weighing balance, filter paper, threads.

Procedure

    1.    Take the given sample and weigh it (A)
    2.    Dry the samples in an oven till there is no more weight loss and weight it (B)
    3.    Grind the sample into powder.
    4.    Place a weighed quantity of sample in filter paper, wrap the paper and tie it tightly with a thread so that no powder comes out of the paper during extraction. 
    5.    Pour approximately 100 ml of anhydrous ether into the bottom flask. Turn the heaters on. Set the temperature at about 40ºC.  
    6.    The ether will evaporate and circulate through the sample and again will come back to the flask. 
    7.    Repeat the process for about 16 – 18 hrs for complete extraction. 
    8.    Remove the sample and evaporate most of the ether from the mixture of crude fat and ether obtained during extraction.
    9.    Evaporate excess of ether from the crude fat sample by evaporation on a water bath. Evaporate till it is free from the fumes of ether. Record the weight (C).

Declaration of facts about the amounts of nutrients required by the various classes of livestockfor differentphysiologicalfunctions like maintenance, growth, lactation, eggproduction, wool productionetc. are in generaltermed as “Feeding standards”. Nutrient requirement is the statement of what animals on an average require for a particular function.Allowanceis greater than the required amount by a ‘safety margin’ which is allowed for variations in requirement between individual animals. Such standards may be expressed in quantities or in dietary proportions, for example gram per day, this method of expression is used mainly for animals given exact quantities of feeds; or gram per kilogram of the diet, this method is used for animals fed to appetite.

Various units are used for feeding standards – for ruminants, the energy requirements may be stated in terms of net energy, metabolizable energy or feed units and protein requirements in terms of crude protein, digestible crude protein or metabolizable protein. It is obviously desirable that the units used in the standards should be the same as those used in the evaluation of feeds. For dairy cows, the requirements are given separately for maintenance and for milk production. But for growing chickens, they are given for maintenance and growth combined. In some instances, the requirements for single functions are not known, for example, vitamins and trace elements. As mentioned above, in feeding practice, the requirement amount is accompanied by the addition of a ‘safety factor’.

I. FILL UPS

1. _____ is the most important primary pests of cereals.
2. _____ causes serious damage in maize cob.
3. _____ is the field pest of pulses.
4. _____ feeds on already damaged.

To Estimate the DNA Using Diphenylamine

Introduction

DNA estimation is helpful to know about the total amount of DNA of prokaryotic as well as eukaryotic system. One method used for DNA estimation is Diphenylamine assay, is that of Burton who modified the original procedure of Dische. This procedure is based on treatment of nucleic acid with hot perchloric acid to hydrolyze DNA nucleotides. Diphenylamine reacts specifically with deoxyribose in DNA to form a blue-colored complex. Since diphenylamine does not react with ribose, one can assay for DNA in the presence of contaminating RNA.

Principle

The DPA method is based on the colorimetry measurement of products formed by the reaction of ù- hydroxylevulinaldehyde (from the deoxyribose released after depurination of DNA) with diphenylamine in 2.5 N perchloric acid.

When DNA is treated with diphenylamine under acidic condition (PCA) a blue complex is formed with a sharp absorption maximum at 595 nm (600 nm). In acid solution, the straight chain form of the deoxypentose is converted to highly reactive ω – hydroxylevulinaldehyde that reacts with diphenylamine to give blue complex. The reaction yields two mixture of product which together have absorbance maximum at 595 nm (600 nm). Interferences include proteins, lipids, saccharides and RNA. In DNA the only deoxyribose of the purine nucleotides reacts, so that the value obtained represents half of the total deoxyribose present.

Litchi (Litchi chinensis Sonn.) is a popular subtropical evergreen fruit tree. The Litchi (lychee) is the most renowned of a group of edible fruits of the soap berry family, Sapindaceae. It is botanically designated Litchi chinensis Sonn. (Nephelium litchi Cambess) and widely known as litchi and regionally as lichi, lichee, laichi, leechee or lychee. Litchi is a tropical fruit tree native to southern China. It is also commonly found in Madagascar, India (Muzaffarpur), Bangladesh, Pakistan, southern Taiwan, northern Vietnam, Indonesia, Thailand, the Philippines, and Southern Africa. Great source of Vitamin C and potassium, it also contains phosphorous, calcium, magnesium and protein. The fruit is liked by everybody because of its excellent quality, attractive red colour and characteristics pleasant flavor.

Varieties

The varieties grown under different states are as below.

Bihar: Shahi, Rose Scented, China, Kasba, Purbi, Early Bedana, Late Bedana

Uttar Pradesh: Rose Scented, Dehradun, Calcuttia

West Bengal: Bombay Green, Kalyani Selection

Punjab: Muzaffarpur, Dehradun, Seedless, Late Bedana

Individuals suffering from the more pronounced types of mental disorder are sometimes said to be insane, but the term insanity is a legal one, not a medical term. An insane person is one who cannot distinguish right from wrong or assume responsibilities for his own actions. In the case of a psychotic patient the whole personality is involved. And he is completely out of touch with real world around. Not so the neurotic patient. Only a small part of his personality is involved. Psychotic patients often require hospitalization; neurotic patients rarely do, unless they have some additional physical problem.

Another distinction between these two major groups is that his friends usually bring the psychotic patient to the doctor, while the neurotic comes of his own accord. He knows he is sick, and he usually wants help. Most often he is suffering from a nervous depression.

1 The clinical manifestations of disease of muscle, bones and joints are generally characterised by

a) Lameness b) Failure of support to stand

c) Incoordination of movement d) All of the above

The main purpose of establishing a nursery is to supply plants whether to one’s own garden or to out station indentors to fetch good income. To obtain adequate returns on the investment made by a nurseryman, one should have fair idea about lifting, packing and storage of the nursery plants.

Lifting of nursery plants

The plants grown in the nursery are to be lifted for establishment at their permanent place or for sale purpose. The careless lifting may affect adversely the establishment of nursery plants. For getting higher rate of success of evergreen plants in the field, it is always advisable to lift the saplings with a ball of earth intact particularly for mango, guava, citrus and litchi etc. However, the deciduous plants like peach, grape, apple, kiwi fruit, hazelnut, pecan nut, cherry, almond, walnut etc. can be lifted bare rooted during dormant conditions. If possible, it is advisable to irrigate the nursery 3-4 days before the actual date of removing the plants in order to prevent root damage. 

Herbicides are chemical substances that are capable of either killing or injuring plants and so used for elimination of unwanted plants. In Latin, the term ‘herba' means plant and ‘caedere’ means to kill. There are many reasons for the increased use of chemicals for weed control. Shortage and high cost of agricultural labour prevent economic control of weeds by manual and mechanical means. Benefits offered by herbicides include efficient weed control, higher crop yield, better soil, water and energy conservation and higher income.

Materials Required

Sensory scoring card, products, pen/ pencil for coding, plain water

Procedure

    1.    Assign codes to the samples i.e. a, b, c, …… or 1, 2, 3, ….. etc.
    2.    Keep the coded samples randomly (eg. c, f, a, b, d, e) in a row for evaluation
    3.    Start the evaluation of samples for various sensory attributes one by one. 
    4.    Evaluator should rinse his/ her mouth after each sample so as to avoid overlapping of taste, flavour etc.
    5.    Assign marks from 1-9 based on pleasurable and un pleasurable experiences.
    6.    Compute the means for a particular attribute on the basis of the evaluation of different evaluators (replications).
    7.    Find out the best sample on the basis of highest average scores obtained.

The total milk production in the country for the year 2013-14 has been estimated at 126 million metric tonnes and the demand is expected to be 180 million tonnes by 2020. To achieve this demand annual growth rate in milk production has to be increased from the present 2.5 % to 5%. Thus, there is a tremendous scope/potential for increasing the milk production through profitable dairy farming.

a. Requirements of project report for setting up dairy units

Project report can be prepared by a beneficiary after consulting local technical persons of State Animal Husbandry Department, DRDA, Dairy Co-operative Society / Union / Federation / commercial dairy farmers.

I. FILL UPS

1. _____ are also called ed-worms.
2. _____ nematode causes discolouration in groundnut.
3. _____ in rice is caused by Aphelenchoides besseyi.
4. The ear cockle disease is caused _____.

Agriculture plays a key role in the overall economic and social well being of the country. Though the share of Agriculture in both GDP and employment has declined overtime, Agriculture still forms the backbone and occupies a pride of place :

1. Agriculture contributes 24 per cent to the Gross Domestic Product. GDP was declined from 39 per cent in 1983 to 25 per cent during 1999-2000 and now it 17.32 per cent.
2. Provides livelihood support for more than half of the population in the country. The sector provides employment to 56.7 per cent of the India’s work force.
3. Agriculture has been the source of raw material for industries. Majority of the industries depending on agriculture for their raw materials. Ex: Textile (Cotton, Jute), Sugar (Sugarcane, Sugar beet), Small & medium scale industries like soaps, dyes, medicines, vitamins, preservation of fruits and vegetables, dhal milling, rice husking, Jaggery making, oil crushing, handlooms etc.
4. It provides large part of market for industrial goods viz., seeds, fertilizers, pesticides, implements, machines like pump sets, trucks, tractors, power tillers, sprayers etc.
5. Support roadways, railways, airways and shipways for transport of inputs & produce.

Eczema is a troublesome skin condition characterized by redness, cracking, oozing and scaling of the skin. Extremes of heat and cold and even emotional stress seem to play a part in this condition. The patient usually complains of itching and burning in the skin, especially at the night. Sores appear on various parts of the body, but most often on the hands, feet, ears and legs. Eczema may come on at any time of life and in either sex. The commoner manifestations are erythema changing to papule, vesicle, crust, scale and fissure, with serous exudation. In chronic cases, thick scales accumulate.

This is the effect of local irritation by chemical, thermal, actinic or mechanical agents in a hypersensitive individual, subject of an allergy or constitutional disabilities such as gout, chronic nephritis, rheumatism, local sepsis, dyspepsia, alcoholism, uterine disturbances also teething. In some cases heredity may be found.

Papules, vesicles and pustules with some degree of local oedema are found; exudation, crusting and scaling with inflammation of varying severity may be present. These tend to spread to the contiguous areas.

One or both cheeks of an infant or the ear, limbs or trunk are occasionally involved. In adults also similar papulo- vesicular eruptions appear. Sensations of burning, smarting, throbbing or itching are present, according to the acuteness of the process and severity of the inflammation.

1 Inflammation of meninges and spinal cord along with brain is called

a) Encephalomyelitis b) Encephalitis

c) Meningitis d) Meningoencephalitis

This process involves building up a coating of sugar and sometimes of other things, layer by layer. Panning is one of those processes that the confectionery and pharmaceutical industries have in common.

The coating of confectionery with sugar using a pan is very ancient. Originally, these products were made in an open pan suspended over a fire by chains. Apparently the operator swung the pan by hand, coating the product with sugar syrup which was then allowed to crystallise. The normal modern small-scale method is to use a rotating dragee pan (Fig.No.19.1).

These are elliptical vessels made traditionally of copper, but now more commonly of stainless steel. The pan is equipped to be rotated and usually there is a system for supplying and extracting air and possibly with a system for spraying in the sugar syrup. Typical pan sizes are between 1.5 and 0.9 m in diameter although smaller sizes are used for small-scale work.

13.1 Incubation
The silkworm eggs are to be incubated properly to avoid irregular hatching. An ideal incubation is to maintain a temperature of 25oC and 75-80% humidity continuously. In most of the places incubators are not available. In such cases, the eggs are kept spread in wooden trays provided with paraffin paper for base and the cover and foam pads kept wet all around inside the trays to maintain 70-80% humidity. In each chawki tray of 90 cm × 120 cm about 4 sheets of eggs can be kept for incubation. The room temperature is maintained at 25°C. On 9th day the egg sheets are rearranged. About 25 dfls (disease free layings) are kept in a chawki tray of 90 cm × 120 cm to enable brushing and proper spacing for young worms later. The egg sheets are covered with a sheet of black paper or paraffin paper. On the due date of hatching and when a few larvae have started hatching, black paper and the paraffin paper are removed and the eggs are exposed to dim light for about an hour. This facilitates the uniform hatching of larvae.
13.1.1 Methods of Incubation
There are two methods, i.e., constant temperature incubation and raised temperature incubation

Materials Required

Sensory scoring card, products, pen/pencil for coding, plain water.

Procedure

    1.    Take the given two samples
    2.    Make them three by duplicating/repeating either one of the two
    3.    Assign codes to the samples eg. AAB, ABA, ABB,  BAB etc.
    4.    Ask the evaluators to evaluate the samples for any differences between the 3 on the given Specimen Evaluation Card.
    5.    Find out how many of the evaluators have chosen the odd sample correctly (say it is 16 out of 20 evaluators).
    6.    Now find out the degree of difference in the samples indicated by the evaluators who correctly found the odd sample (Say it is much as per 3, moderate as per 8 and slight as per 5 out of the 16 correct evaluators.).
    7.    Find out how many of the correct evaluators preferred A sample and how many the B sample (say 11 preferred A sample and 5 B sample).
    8.    After going through Annexure II you may conclude that the two samples differed significantly from each other as 16 out of 20 evaluators found the odd sample correct. But, the preference was not significant as it should have been at least 13 out of 16 judges indicating same preference so as to make it significant. So, it may be concluded that the two samples differ significantly but both are acceptable.

ABSTRACT

An In vitro study was conducted to evaluate the fungicidal efficiency of four selected fungal antagonists against leaf spot pathogens of ivy gourd by dual culture method. The antagonists evaluated were Trichoderma viride, Trichoderma harzianum, Aspergillus niger and Chaetomium globosum. The major leaf spot pathogens of ivy gourd used for the study were Cercospora cocciniae, Colletotrichum gloeosporioides and Alternaria alternata, causing severe infection on the foliage. Among the four antagonists tested, T. viride and T. harzianum were found to be the most effective recording the highest AI value of 1500 against all the three pathogens followed by A. niger. C. globosum was least inhibitory on C. gloeosporioides and A. alternata, but was effective against C. cocciniae. A complete overgrowth of the antagonists on all these pathogens were observed in the case of T. viride, T. harzianum and A.niger. C. globosum showed aversion to the hyphal growth of C. gloeosporioides and A. alternata, whereas in C. cocciniae, a homogenous growth was observed.

Soil Reaction

Soil reaction is an indication of the acidity or basicity of the soil and is measured in pH units. The pH scale ranges from 0 to 14, with 7.0 as neutral point. From pH 7 to 0, soil is increasingly acidic. From pH 7 to 14, soil is increasingly alkaline.

Pure H₂O ionizes to a very small degree, though to a measurable extent, so that it has the following equilibrium: both H+ and OH^- are in concentrations of 10^-7 moles L^-1.