eChapters

Introduction

Among the rice growing countries, India has the largest area (448 lakh ha) followed by china and Bangladesh. Total food grain production in India is estimated at 2813l.7 lakh tonnes. Rice production is estimated at 1156 lakh tonnes (Anonymous, 2010). In India, rice is grown in the state of Kerala, Andhra Pradesh, Orissa, Bihar, Uttar Pradesh, Madhya Pradesh, Punjab and West Bengal lead in the area, while West Bengal harvest highest rice production. The average yield per hectare is highest i.e., 5970 kg/ha in the Punjab during 2018-19.

Rice is grown throughout the state except Kachchh and Saurashtra. Some part of the state is growing rice as a drilled, while transplanted rice is taken in south and middle Gujarat. In Gujarat rice occupies about 10.61 per cent of the gross cropped area of the state and accounts for around 25.5 per cent of the food grain production. It is grown on an average about 7.5 to 8.5 lakh hector of land comprising nearly 70 to 80 per cent low land (transplanted) and 15 to 20 per cent of upland (drilled) rice (Anonymous, 2020). During the 2018-19, the yield of kharif rice in Gujarat was around 2247.75 kg/ha. Yield of summer rice was 3130.96 kg/ha and total production of rice in the year of 2018-19 was 1911 tonnes (Anonymous, 2020). During the 2019-20, the yield of kharif rice was 2117.81 kg/ha and yield of summer paddy was 2996.36 kg/ha and total production of rice in the year of 2019-20 was 1929.98 tonnes (Anonymous, 2020).

In Gujarat some districts like Chhotaudepur, Tapi, Navsari have highest rice productivity which covered 1.279 lakh hector area with production and productivity respectively of 3.338 lakh tonne and 2582 kg/ha and some district like Mehsana, Narmada, Panchmahal, North Gujarat etc. have medium to low productivity of paddy. But in some part of North Gujarat like Gandhinagar and Sabarkantha have low productivity of rice.

Natural goat farming is one among various farming systems that are close to nature and sustainability. In Uttrakhand which has 13-15 lakh goats as per animal husbandry department goat farming or rearing mimics how an animal would behave if they were freely living in the wild which therefore provides a more humane experience for the animal.In Uttrakhand, districts like Uttarkashi, Joshimath etc goat rearing is a part of indigenous lifestyle and tradition and is passed down generationally. They operate on no cost or low cost models therefore by default it is close to natural farming. They allow their goats to graze in forests and bugyal which are free from pesticides and fertilizers.Natural goat farming shall aim to use natural breeding methods, minimize stress, taking preventative measures and thereby eliminate the use of allopathic drugs or minimalist use of drug and detain animal health and welfare.Breed selection in natural goat farming should be indigenous as it is genetically adapted to specific or extreme conditions. They will be more productive and production cost will be lower.

Aquatic plants are recognized as a valuable forage crop, ideally utilized in their fresh state, dried as hay, or preserved as silage. Their suitability and profitability vary depending on the type of livestock being fed. These crops do not occupy arable land and require no investment for cultivation; however, there are costs associated with harvesting. Feeding aquatic plants directly in their natural habitat is infrequently feasible due to factors such as water depth, risks posed by mud and pathogens, and potential contamination of the water by livestock.
1. Water Hyacinth (Eichhornia crassipes): Water hyacinth is a perennial herb that floats freely on the surface of freshwater, including rivers, lakes, canals and ponds. In India, the dried form of water hyacinth or its ensilage is utilized as feed for cattle, owing to its high protein content. The amino acid composition of water hyacinth renders it an appropriate feed option for pigs as well. Typically, the plant is chopped and fed directly to livestock; however, its palatability is compromised by the presence of oxalate crystals in the leaves, which can cause oral irritation in animals. Additionally, cattle may experience adverse effects such as diarrhea and increased urination when consuming water hyacinth. This issue can be mitigated by supplementing the diet of adult cattle with groundnut and paddy straw alongside water hyacinth. Ensiling is an effective preservation technique that improves the palatability of water hyacinth. Due to its high moisture content, it is advisable to wilt the plant in the shade for 48 hours, or alternatively, prepare silage by mixing it with straw in a 4:1 ratio. The addition of molasses is to enhance the soluble carbohydrate content. The DCP and TDN in the silage are measured at 5.64% and 40.26%, respectively. The nutrient composition of water hyacinth includes 5.80% DM, 18.30% CP, 22.60% CF, 2% EE, 21.10% TA, 9.50% Ca and 6.30% P. The nutritive value of fresh water hyacinth is 7.66% DCP and 43.73% TDN, with oxalic acid content ranging from 2.50% to 3.60%

Nanometre : The unit of length specifically applied to wavelengths of radiation. This is equal to ten Angstrom units or 10-9 meters.

Net Assimilation Rate (NAR) : It is increase in dry weight of plant per unit leaf area per unit time. NAR is calculated from the following equation : 

1. Genetic principles of seed production were given by

   a) Feistritzer (1975)

   b) Kadam (1942)

   c) Kadam (1936)

   d) Swaminathan (1970)

2. Objectionable weed seed in wheat seed production is

   a) Chandvel

   b) Johnsoon grass

   c) Sudan grass

   d) Wild rice

An inflammation of the muscular substance of the heart. The condition may be acute or chronic. Acute myocarditis may be sub-divided into three varieties:

1. Acute Parenchyma.
2. Acute Diffuse Interstitial.
3. Acute Circumscribed.

In the first of three classes, there is a glandular degeneration of the muscular fibres of the parenchyma of the heart and an increase in the number of their nuclei. Severe cases of this class often terminate in fatty degeneration.

In the second class, we have round-cell infiltration of connective tissue of the myocardium.

Introduction

Karonda is an evergreen shrub or short stature tree. It grows naturally in the Himalayas and Western Ghats at elevations of 300 to 1800 meters. It is found grown in wild in India, Malaysia, South Africa. It is a well suitedto arid climate and grown well at higher temperature. It is commonly uses for making hedge for orchards. It is suitable for the arid tropics and subtropics and is grown for its attractive, edible fruits. It also grows successfully on marginal and wastelands. It thrives well as a rainfed crop; the plant hardly needs any care and gives yield with the minimum management. The plants can be trained as a strong hedge. Karonda is suitable for growing throughout the sub-tropical and tropical climatic zones of India. Major natural areas of occurrence of karonda have been observed in the states of Maharashtra, Bihar, West Bengal, Chhattisgarh, Orissa, Gujarat, Madhya Pradesh, Rajasthan and in the Western Ghats. In Maharashtra, the major area under this crop is scattered in sub-mountain areas like Kolhapur, Ratnagiri, and Pune district.

The selection of the site has great importance for the establishment of a fruit orchard successfully. Establishment of orchard is a long term activity with huge investment. Hence, any mistake committed during site selection, it is difficult to rectify it later. Successful orchard is not only related with yield but also its economic production and efficient disposal of produce. For economic orchard establishment; some of the points which need to be considered, while selection of site. These are as follows.

1. Climate and soil: These are two important natural factors, which decide the success or failure of orchards. Before selection of site, climate and soil requirement for selected fruit crops needs to be studied thoroughly and accordingly site must be selected. On the other hand if a site has already been selected; then fruit crop selection needs to be done as per the existing climate and soil of that selected site.
2. Topography: Slope of site more than 15% makes the land almost unsuitable for cultivation. Land with 4% slope may be kept clean without much loss. Uneven land involves heavy expenditure in leveling. Southern slopes are generally warmer and affect the plant growth in a manner different from the northern slopes. It is due to the difference in quantum of receiving sunlight. Hence, the site with southern slopes may be selected for sun loving plants.

29.1. Aim

To estimate ascorbic acid in fresh and processed fruit and vegetable products.

29.2. Theory and Principle

Fruit, vegetables and their products are important sources of ascorbic acid. The ascorbic acid is present in sufficient quantity in aonla, guava, grapefruit, lemon, pineapple, strawberry fruits etc. Barbados cherry is the chief source of vitamin C. The products manufactured from these fruits are also considered as rich in ascorbic acid and the contents available in the commodities can be detected by using 2, 6 dichloro-phenol - indophenol visual titration method.

The genera Proteus and Providencia belong to the tribe Proteae of the family Enterobacteriaeceae. The members of both these genera are gram negative, motile bacilli, aerobes and facultative anaerobes and can grow on basic media. A characteristic feature which distinguishes tribe Proteae from other members of Enterobactereaceae is the presence of the enzyme phenylalanine-deaminase which converts phenylalanine to phenylpyruvic acid (PPA reaction).They also produces a powerful urease enzyme which rapidly hydrolyses urea to ammonia.
Genus Proteus
They are gram-negative bacilli, 1-3 μm long and 0.6 μm wide. They are noncapsulated and are actively motile by peritrichous flagella. The name ‘Proteus’ refers to their pleomorphism, after the Greek God Proteus who could assume any shape. Four species: Proteus mirabilis, P.vulgaris, P.penneri and P.myxofaciens are recognized. Proteus mirabilis, P.vulgaris are widely recognised as human pathogens.
Culture Characteristics
These can grow on ordinary media like nutrient agar with a characteristic fishy or seminal odour. On MacConkey and Teepol lactose agar, lactose nonfermenting pale colonies, around 2-3 mm in size are formed. On non-inhibitory solid media such as blood and nutrient agar Proteus mirabilis and P vulgaris show characteristic swarming growth in the form of a uniform film, which spreads over the whole surface of the plate. In young swarming cultures, many of the bacteria are long, curved and filamentous, sometimes reaching upto 80 μm in length. When two different strains of swarming proteus mirabilis encounter one another on an agar plate, swarming ceases and a visibile line of demarcation forms. This is known as the Dienes phenomenon. In liquid medium (peptone water, nutrient broth), Proteus produces uniform
turbidity with a slight powdery deposit and an ammoniacal odour.

Oasis effect : Vertical energy transfer from the air to the crop. It is the exchange of heat between a growing crop and hot air whereby air over the crop is cooled.
 
Observation : In meteorology, the evaluation of one or more meteorological elements, such as temperature, pressure, or wind, that describe the state of the atmosphere, either at the earth’s surface or aloft. An observer is one who records the evaluations of the meteorological elements.
 
Occluded Front : Also known as an occlusion, it is a complex front formed when a cold front overtakes a warm front. It develops when three thermally different air masses conflict. The type of frontal boundary they create depends on the manner in which they meet.

Botany: French bean has tap root system with poor nodule formation. Leaves are trifoliate. Though a self-pollinated crop, French bean offers wide variability for plant growth (bushy / climbing), colour of pod (green / waxy coloured), cross section of pod (flat / oval / round), pliability (stringed / string less) etc.

Varieties

1. Arkakomal: Plant is bushy. 70 days for flowering.
2. Blue pod medium: It is a white seed variety. It has bluish black spots on pods.
3. Bountiful: Introduction from US comes up in September to February. Seeds are Brown sweetish. It is resistant to common disease.
4. Contender: Tolerant to powdery mildew and mosaic.
5. Pant anupama: Developed from pantnagar. Bushytype
6. Pusaparvathi: Developed by X ray radiation of American variety IARI

To isolate a streptomycin resistant mutant of E.coli by Direct selection using Gradient Plate Method.

Introduction

Zylbalskii devised this technique.

A mutation is any change in the nucleotide sequence of DNA. These changes are heritable. Although most mutations are harmful to the organism, a small percentage is beneficial under certain environmental conditions. Mutation can occur during chromosome duplication and they may be spontaneous of they may be induced by mutagenic agent. In this experiment, we select for a mutation causing streptomycin resistance in E. coli. Resistant mutants will be detected directly using thy gradient plate method.

Principle

Microbiologist developed a highly efficient method for isolation of antibiotic resistant mutant. The gradient is said as gradient of antibiotic formed on an agar plate. A gene imparting resistant toward an antibiotic is present in plasmid or chromosome. The occurrence of mutant with different level of streptomycin resistant involves mutation of gene with unequal potential. This means that under certain cells degree of mutation is more while few shows less mutation. Some cells show intermediate resistant while others show completes resistant. The resistant in cell against streptomycin of this type, while in case of penicillin resistant increases step arise & so resistant mutant of high concentration of penicillin cannot be isolated/ obtained. While it is possible with streptomycin obtaining high resistance to penicillin stepwise & so this stepwise increase in resistance involves mutation of gene with equal potential. This theory of mutation through multi step procedure. The gene direct the organism to produce an enzyme, which inactivates an antibiotic & thus resistance to antibiotic, is observed in case of cells. Resistance of antibiotic is a plasmid-mediated resistance.

Tamarind (Tamarindus indicus L.) belongs to the family Fabaceae. Genus Tamarindus is a monotypic taxon having only one species. Tamarind is native to tropical Africa, probably Sudan, where it grows wild and is cultivated throughout South-East Asia and America. It is also found growing in Camaroon, Nigeria and Tanzania. Tamarind has a long gestation period of 10-20 years and long-life span of even 100 years.

Cultivars/Varieties: In some regions the type with reddish flesh is distinguished from the ordinary brown-fleshed type and regarded as superior in quality. There are types of tamarinds that are sweeter. One in Thailand is known as ‘Makham waan’. Another one is known as ‘Manila Sweet’. No other improved or named varieties are available.

Uses

Tamarind pulp is an important condiment as a sour ingredient in the Indian cuisine. Pulp has an excellent keeping quality. Timber is used for making agricultural implements. The wood yields excellent charcoal and is used for making gunpowder. Starch is obtained from seeds and is used in the textile industry. Seeds can be fed to animal after roasting and removing the outer skin. Seed is also a good source of tanning. Oil is obtained from seeds and is used in paints and varnishes. Leaves and flowers are also edible. It is rich in medicinal properties. Fruits is considered as digestive, carminative and laxative. With the addition of water and sugar, tamarind yields a cool drink which is quite popular in Latin America.Today India is the largest producer of tamarind in the world.

Microbes that live inside plant tissues without causing disease are termed as endophytes. Endophytes have been found in all parts of plant viz., seeds, roots, stems, leaves, fruits etc. Within these organs, there is evidence of endophytes living in apoplastic intercellular space within parenchyma tissues and xylem vessel apoplast. Endophytic bacteria have been isolated from both monocotyledonous and dicotyledonous plants, ranging from woody tree species to herbaceous crop plants. Further, a single plant may be a host for several different bacterial species. They are consistently present in the root, stem, leaf, fruit and tuber tissues of a wide range of agricultural, horticultural and forest species. All plants in nature harbor a diverse community of endophytic bacteria which can positively affect host plant growth.

The potential of endophytic bacteria to fix nitrogen, promote plant growth and suppress plant diseases has renewed interest in such associations. It is advantageous to have the endophytic plant growth promoting organisms safely inside the plant, which could supply substantial amounts of nitrogen and other nutrients, growth hormones and other beneficial effects to the crop plants without the interference of the rhizosphere soil environment. Furthermore, plant growth promotion is often greater when it is induced by endophytes rather than by rhizosphere bacteria.

It is very important to watch weight, not allowing oneself to become too thin or too fat, but trying to remain neat ideal. Most youngsters in their teens, if they are healthy and active, are fairly close to the ideal weight for their size and age. Changes often come in the late twenties, reducing a person's physical fitness. Sluggish habits begin to develop as the individual loses his skill in running, swimming or healthy activities.

When greater quantities of energy (in the form of food) enter the body than are expended, the body weight increases. Therefore, obesity is caused by excess energy input over energy output. In other words, extra weight is due to extra eating. For each 9.3 Calories of excess energy that enters the body, one gram of fat is stored.

Excess energy input occurs only during the development phase of obesity. Once a person has become obese, all that is required to remain obese so that the energy input equals the energy output. For a person to reduce in weight, the input must be less than the output. People with large bones and well-developed muscles may seem somewhat overweigh compared with those of lighter build. But actually the lighter person may carry around more fat. His muscles may be weak and underdeveloped.

Biofilms play an important part in biotechnology, immunology, biofouling and biodeterioration (Fleming and Wingender, 2001; Dunne, 2002). When the pathogenic cells adhere to the surface, it has been found that the transcription of the EPS genes get stimulated (Dunne, 2002). If EPS gene expression are monitored it can provide a good understanding of biofilm phenotypes (Allison and Sutherland, 1997; Helm and Naumann, 1995; Petersen et al., 2005). EPS serves many functions. It also enables the bacteria to capture nutrients (Pontefract,1991).The formation of biofilm goes through a mechanism which is different from species to species. Even the matrix thickness can be different from 0.2 to 1.0pm depending on the species and doubling time of the cells as thickness of 10 to 30 nm has been found in some bacteria species (Sleytr,1997). The cells secrete polysaccharides, proteins, nucleic acids and glycoproteins. Adherence on solid surfaces by microorganisms is a very complicated process that depends mostly on extracellular molecule production (Czaczyk and Myszka, 2007). The biosynthesis of EPS provides an optimal environment in which genetic material between the cells can be exchanged. This is needed for cell identification and the antigenic properties are helpful in serological characterization of the cells. Among the various compounds of extracellular carbohydrates are acetate esters, pyruvtes, formates, succinates, polysaccharides and proteins (Sutherland, 2001a).

Testicular Tumours

Seminoma

• Cells were round exhibiting anisocytosis
• Round to oval nuclei
• Single distinct eosinophilic to basophilic nucleoli
• Cytoplasm scanty

Sertoli Cell Tumours

• Cellularity - High, anisocytosis
• Nucleus round to oval, megakaryosis, 1-3 basophilic nucleoli
• Chromatin coarse and granular
• Cytoplasm vacuolated

Q1. Penicillins disrupt the formation of following peptide bond during its antibacterial action on bacteria:

1. D-alanine-D-aspartate
2. D-alanine- D-glutamate
3. D-Glycine-D-alanine
4. D-alanine-D-alanine

Q2. In E.coli, topoisomerase IV, the target enzyme of the quinolones is encoded by the genes

1. parC and parE
2. gyrA and gyrB
3. par C and gyr A
4. parE and gyrB

Q3. Streptomycin interferes in protein synthesis by inhibiting the start codon

1. AUG
2. AAU
3. UUA
4. AGUa

30.1. Aim

To estimate the salt contents in given samples by using Mohr method.

30.2. Theory and principle

Common salt is used during preparation of pickles, sauces, ketchup, fruit chutney and appetizers. Besides, imparting taste, the salt in appropriate concentration also acts as a preservative. In different products, it is estimated by using Mohr method. The sample is titrated with standard solution of silver nitrate for estimation of salt by using potassium chromate as an indicator. From the titre the concentration of salt is estimated by using a standard expression.