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Hybridisation

Hybridisation occurs when two species cross to form a new species. If they are named before their hybrid nature has become apparent, the names would become invalid as soon as the hybridity of the specimen has been established.

Hybridism can be of three types:

1. Sympatric Hybridisation

When individuals in two regions overlap in an otherwise well-defined species, they are called sympatric hybridisation. This is common in bird of paradise and humming birds. This hybridisation is listed as cross of two parental species.

Sulphur dioxide is used as a preservative in many fruit and vegetable products. It can be added either in the form of potassium metabisulphite or by exposingthe product to sulphur fumigation by burning sulphur powder. Food safety and standards regulations have specified the maximum limit of sulphur dioxide in the finished products. Products having sulphur dioxide content exceeding the limit are considered not conforming to Indian Food Laws. Therefore, the estimation of sulphur dioxide in food products is considered an important attribute for quality evaluation.
Principle
Sulphur dioxide added to the food products may exist as undissociated sulphurous acid, as free bisulphite ions, as free sulphite ions and/or as combined SO2 in the form of hydroxy sulphonates. Sulphur dioxide is measured as free or total SO2.
Free SO2 is measured by direct titration with iodine. In the estimation of total SO2, the combined SO2 is liberated by treatment with excess alkali at room temperature, followed by subsequent acidification to prevent recombination and titration with iodine. The method used is known as the Modified Ripper Titration Method.

Botany: The Cocoa (Theobroma cocoa) belonging to the family Sterculiaceae. Cocoa tree is a shade tolerant, moisture loving, understory rainforest tree. The trees live for up to 100 years, but cultivated trees are considered economically productive for only about 60 years. Naturally Cocao grows to a height of 15 meters, but cultivated trees are trimmed shorter to make harvesting easier. The leaves of Cocoa are smooth bright green, oblong, about 15cm by 8cm. The seeds are encased in a large colourful pod which grows close to tree after a flower. The large pod is green while maturing and turns yellow, orange, red or purple when ripe. They range from about 10 cm to greater than 40 cm in length.

Soil and climate: Potash rich alluvial soils friable in nature with high humus and moisture retentivity with a pH of 6.6-7.0 are suitable. Cocoa is normally cultivated at altitudes upto 1200 m above MSL with an annual rainfall of 150 cm and a relative humidity of 80 % and annual mean temperature of 24^oC to 25^oC. Cocoa can be grown as intercrop in coconut and arecanut gardens.

Facilities required for propagating many plant species by seed, cuttings, or grafting and budding include two basic units namely a structure with temperature control and ample light such as a greenhouse or hot bed where seeds can be germinated or cuttings rooted. The second unit is a structure into which the young tender plants can be moved for hardening preparatory to transplanting and cold frames or lath houses are useful for this purpose.Greenhouses: There are different types of greenhouse namely low cost greenhouse and commercial greenhouses. In all polyhouses/ greenhouses means of providing air movement and air exchange is necessary to aid in controlling temperature and humidity It is best, if possible, to have in the greenhouse heating and self-opening ventilators and evaporative cooling systems. Plastic greenhouses covered with various types of plastic film have become very popular among nursery men.

42.1. Aim

To conduct practical on preparation of chutney from different fruits.

42.2. Theory

Fruit chutney is made in the same way as jam except that the spices, salt and vinegar or acetic acid is also added. In comparison to jam, chutney contains less total soluble solids. The product shall be of good quality with palatable and appetizing taste. As per FSSAI specifications, the chutney shall contain minimum of 40% fruit (w/w) in the final product with total soluble solids not less than 50%. The acidity in the final product shall not be more than 2 percent with ash content not exceeding 5 percent. Mixed fruit chutney is commercially made by using different fruits in appropriate combinations.

42.3. Raw material, ingredients, equipments and utensils required

1. Stainless steel peeling/cutting knives, pulper for extraction of pulp.

2. Utensils for cooking and mixing, ladle, glass jars, sterilization tank, diesel or LPG stove/bhatti etc

As it is well understood, food and drinking water are the first among the hierarchical needs of a human being. Growing population, expanding ecological footprint, diminishing per capita land and water availability, increasing biotic and abiotic stresses, and, above all, the prospects for adverse changes in temperature, precipitation and sea level as a result of climate change, emphasise the need for keeping issues relating to agriculture high on the professional, political and public agenda. The multiple roles of agriculture in food production, improving nutrition and health, and climate change mitigation are now well recognised scientifically, but are yet be integrated into coherent national policies and strategies. Opportunities for generating synergy among agriculture, nutrition and health are great and this conference is therefore a timely one.

Preserves (Murabbas) are prepared from whole fruits and vegetables or their segments by addition of sugar followed by evaporation to a point where microbial spoilage cannot occur. The final soluble solids concentration is reached to about 70 per cent. The finished product can be stored with or without hermetic sealing and refrigeration.

Aim

To determine the thermal conductivity of the agricultural materials probe method.

Materials required

Thermal conductivity probe

Stainless-steel sample container

Grain or powder sample

Graph sheet

Power source

Tetanus is a specific infectious disease caused by the exotoxin of tetanus bacilli, usually growing in necrosed tissues, absorbed by the motor end plates of the peripheral nerves, carried to the central nervous system and manifested as lockjaw and paroxysmal spasmodic contraction of other muscles.

Tetanus bacilli present in the soil especially one contaminated by faeces of a horse or of a similar animal, enters through some skin abrasions and remain localized at the site. These are thereafter removed by phagocytes but if any lacerated tissue is present, more so if contaminated by pyogenic bacteria, these multiply and manufacture a highly potent exotoxin which is absorbed through the motor nerve end-plates in the muscles carried to the motor cells of the central nervous system, the sensory ones not being affected.

The posterior root ganglia act as filter preventing the toxin from entering into the spinal cord, the anterior root is thus only affected.

The proper supply of feed and fodder is a requirement for the growth and development of the animals. Over the past few decades, there has been a considerable growth in milk production, with India now producing the most milk in the world (187.7 million tones in 2018–19). The main causes of India’s low milk yield are extrinsic (poor nutrition/feed management) as well as intrinsic (low genetic potential).

The young seedling stock and rooted cuttings as well as nursery plants require proper care and nourishment. The operations to be performed regularly in nurseries are as follows.

1. Shifting of plants: The nursery plants grown in the pots or polybags are to be shifted once in 2-3 months to avoid root penetration into the soil. If the taproot penetrates deep into the soil, the chances of mortality increases. Hence shifting of grafts and potted plants has to be done at the said intervals
2. Removal of rootstock sprouts: Once has to keep a constant watch on the sprouts arising on the rootstock, which are to be nipped off as and when, observed, to encourage rapid growth of scion shoots.
3. Irrigation: The young tender seedlings are exacting in their water requirement. Proper and judicious irrigation is an important indicator of a healthy nursery, which solves many problems in the nursery. There are many methods adopted in nurseries. However, it depends upon water availability, kind of nursery plants and its growth stage, etc. These methods are flow or flood irrigation, furrow irrigation, overhead irrigation through rose cans, automizers or sprinkler systems, etc.

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43.1. Aim

To conduct practical on preparation of preserve and candies from fruits.

43.2. Theory

Preserves (murraba) are made by cooking the entire fruit or its slices/pieces in sugar syrup of higher concentration (65-70°Brix). Usually for 1 kg fruit around 1.75 kg sugar is used and the cooking is continued till the TSS reaches 68%. Fruits like aonla, bael, apple, pear, karonda, pineapple and papaya etc can be used for making preserves. Aonla, bael and apple preserves are commercially available in the market.

Candies are also prepared in similar way as preserve, but the TSS maintained is slightly higher (75-80%). In preserve, the fruit is kept immersed in syrup and packed while in case of candies, the syrup is drained off and fruit pieces are further dried and packed. For candy preparation, the fruit pieces are dipped initially in 55-60% sugar solution and the TSS of the syrup is increased by 5% everyday up to 4-5 days till a TSS of 70oBrix is reached. The fruit pieces are then separated from syrup, rolled in powdered sugar, dried and packed. Karonda, apple, peels of orange, lemon, grapefruit, petha (Ash gourd) and ginger etc are used for candy preparation. Petha, ginger, papaya, aonla etc candies are commercially available in the market.

The term canning refers to a process that involves heating foodstuff in hermetically sealed containers for a specific time at a specific temperature to eliminate microbial pathogens that endanger public health; and microorganisms and enzymes that deteriorate food during storage.
Principle of canning: Destruction of micro-organisms causing spoilage, within the sealed containers using heat.
Canning Process
Preparation of food commodities for canning consists of selection of commodities, washing, sorting, grading, peeling, halving, blanching, filling into cans, filling hot syrup or brine, exhausting, double seaming, Sterilization/ processing, cooling, storage, etc.
Raw material selection
Fruits should be ripe but firm, evenly matured, and free from blemishes, insect damage, and malformation. Apple, pear, peach, and banana harvested at the mature stage are preferred for canning.

Moraxella bovis occurs as short (1.0 to 1.5 x 1.5 to 2.5 μm), plump Gramnegative rods or, occasionally, cocci which typically occur in pairs. This organism is non-motile, aerobic and usually catalase-positive and oxidasepositive. Although proteolytic, it is unable to utilize sugars. Growth, which is enhanced by the addition of blood or serum to media, does not occur on MacConkey agar. The optimal temperature for growth is 33-35°C. Most M.phenylpyruvica strains will grow on MacConkey agar. But M.bovis and M.lacunata are unable to do so.
Virulent strains, when isolated from cases of infectious bovine keratoconjunctivitis, are fimbriate, haemolytic and grow into the agar. Species, other than M.bovis, which are periodically isolated from clinical specimens, are generally regarded as non-pathogenic.
Usual Habitat
Moraxella bovis is found on mucous membranes of carrier cattle. The organism is susceptible to desiccation and is short-lived in the environment. It can survive for up to 72 hours in the salivary organs and on the body surface of flies, which can act as vectors.
Colonial Appearance
On blood agar after 48 hours’ incubation the colonies of M.bovis are flat, round, small (1 mm diameter), greyish-white and friable, surrounded by a narrow zone of complete haemolysis. The appearance is not unlike that of a beta-haemolytic streptococcus. New isolates are often piliated and erode the agar, sinking into it. Colonial growths will autoagglutinate when suspended in saline. On subculture, colonial variation is common, pili are no longer formed and the colonies are butyrous and less likely to autoagglutinate. Some colonies can become non-haemolytic. The strains of M. hovis (M.equi) isolated from
horses are non-haemolytic even on primary isolation. M.lacunata and M. phenylpyruvica are non-haemolytic on blood agar and some strains of M. phenylpyruvica will grow on MacConkey agar.

This study examines how various socio-economic variables affect migration distance and happiness among toto-pullers in farm ecology, analysing 15 independent variables and their relationships with six dependent variables. The distribution patterns show moderate variability in family size, income, and material possessions, with notable variability in factors such as migration costs and water scarcity. Correlation analyses reveal that higher education, income, and land holdings are negatively correlated with migration distance, while larger family size, older average age, and better housing conditions correlate positively with longer migration distances. Regression analysis indicates that family size significantly influences migration distance, while higher education levels are associated with shorter distances. Stepwise regression confirms family size as a key predictor, explaining 20.7% of the variance in migration distance. Path analysis highlights that larger families directly increase migration distance, whereas higher education leads to shorter distances. In terms of happiness, material possession, house type, and income from livestock are positively correlated, suggesting that better living conditions significantly enhance happiness. Conversely, higher migration costs and lower education and income levels are negatively correlated with happiness. Overall, the findings underscore the critical roles of family size, socio-economic conditions, and material factors in shaping migration patterns and happiness, indicating areas for targeted interventions to improve migration outcomes and overall well-being among toto-pullers. The stepwise regression analysis identifies several key predictors of unhappiness, migration benefits, push factors, and pull factors. Material possession (x7) emerges with a negative coefficient of -0.224 and a t-value of -1.725, suggesting that while higher material possessions are somewhat linked to reduced unhappiness, this effect is not statistically significant. In contrast, total annual income from livestock per capita (x12) significantly reduces unhappiness, with a coefficient of -0.360 and a t-value of -3.826, reflecting a robust negative relationship. Family size (x1) also significantly influences unhappiness, with a coefficient of -0.299 and a t-value of -2.537, indicating that larger family sizes are associated with greater unhappiness. The model explains 51.6% of the variance in

Transgenic varieties combine genes from totally unrelated species. For example, we can now transfer genes for salinity tolerance to rice from mangroves. The recombinant DNA technology is part of the evolution of genetics starting with the rediscovery of Mendel’s Laws of Inheritance in 1900. In the early part of the twentieth century, various techniques like irradiation, use of chemical mutagens and doubling of chromosomes through colchicine treatment were adopted to develop novel genetic combinations.  Today such gene transfer can be done with both precision and ease through recombinant DNA technology. Both molecular marker-assisted breeding and gene transfer now play a very important role in developing genetic combinations to meet the challenges rising from biotic (i.e., pest and diseases) and abiotic (i.e., drought, flood, sea level rise, etc.) stresses. They will gain further importance in the emerging era of climate change. Varieties developed by marker-assisted selection are also eligible for certification under the protocol for products produced through organic farming.

Nanoparticle characterization is necessary to establish understanding and control of nanoparticle synthesis and applications. Characterization is done by using a variety of different techniques, mainly drawn from materials science. Common techniques are electron microscopy (TEM, SEM), atomic force microscopy (AFM), dynamic light scattering (DLS), x-ray photoelectron spectroscopy (XPS), powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF),ultraviolet-visible spectroscopy and dual polarisation interferometry.

Whilst the theory has been known for over a century (see Robert Brown), the technologyfor Nanoparticle trackinganalysis (NTA) allows direct tracking of the Brownian motion and thismethodtherefore allows the sizing of individual nanoparticles in solution.

Botany: Coffee trees are evergreen shrubs of the rubiaceae family. Coffee of commerce consists of seeds of coffee which when roasted, ground and soaked in hot water yields a fragrant stimulating infusion used for preparing the beverage called Coffee. It is native of America and introduced in India in 1936 near Chickmanglur in Karnataka.

Coffee plants have bright green opposite leaves with smooth margins. Its flowers, white, grow in clusters in the axils of the leaves and are aromatic. From them, fruits are born.They are red drupes with the size of a cherry The outside of the fruit is fleshy and inside it there are two seeds or coffee beans, surrounded by a membranous layer of leathery texture, hence this layer is commonly known as the “parchment”. Some species of trees only produce one seed per fruit are known as “pearl coffee”. In double seed varieties, the two seeds that grow inside each fruit press each other, so they stop the growth in the part which is in contact, so this becomes flat.

Soil and Climate: Coffee arbica comes up well in high altitude from 800 to 1650 meters but -C. canephora Pierre ex A.Froehner adopts well to lower elevations. At high altitude the crop is often late and susceptiable to frost and high winds.Evenly distributed rainfall of 2250 mm is essential. Heavy rainfall is not counducive as it encourages rotting of leaves and plants are easily susceptible to fungal diseases.

Although the Dacron bag technique for protein degradation measurement is relatively simple and not too expensive as compared to in vivo measurements, the technique is not easily adaptable in laboratories that are not fully equipped to maintain cannulated animals. Therefore, different alternative approaches such as measurement of protein solubilities in buffer, enzymes and detergent solutions have been tried (Pichard and Van Soest, 1978; Krishnamoorthy et al., 1982, 1983, 1995; Aufrere et al.,1991; Prabhu et al., 1996; Licitra et al., 1996 and 1998). The advantages of these approaches are that, they are simple, rapid, economical and repeatable. However, since the enzymes used are not of rumen microbial origin, degradation obtained from these enzymes may not be a true .reflection of degradation occurring in the rumen. Nevertheless, because of their simplicity and rapidity, in situations where large number of samples are to be compared, in vitro techniques serve as useful tools.