eChapters

1.    Rust
        
2.    Cercospora leaf spot
        
3.    Collar rot and Root rot
        
4.    Phyllody

1. ICAR-Central Island Agricultural Research Institute, Port Blair

2. ICAR-Central Arid Zone Research Institute, Jodhpur

3. ICAR-Central Avian Research Institute, Izatnagar

Aim: Perform an experiment to determine the parameters of a three-phase induction machine.

As the circuit modal of an induction motor is similar to that of a transformer, the parameter of the modal can be obtained by no-load test and blocked rotor test.

No-load test

In this test, the motor is to run at no-load at rated voltage and frequency. The applied voltage and current and power input is measured. Power input at no load (Po ) provides only the losses that are rotational losses.

Confocal microscopy is a powerful tool that creates sharp images of a specimen that would otherwise appear blurred when viewed under a conventional microscope. This is achieved by excluding most of the light from the specimen that is not from the microscope’s focal plane.The image thus obtained has less haze and better contrast than that of a conventional microscope and represents a thin cross-section of the specimen. Laser scanning confocal microscopy has become an invaluable tool for imaging thin optical sections in living and fixed specimens ranging in thickness up to 100 micrometers. In fact the confocal microscope is often capable of revealing the presence of single molecule.
The modern confocal microscopes can be considered as completely integrated electronic systems, where the optical microscope plays a central role in a configuration that consists of one or more electronic detectors, a computer (for image display, processing, output, and storage) and several laser systems combined with wave length selection devices and a beam scanning assembly. In most advanced systems, integration between the various components is so through that the entire confocal microscope is often collectively referred to as a digital or video imaging, capable of producing electronic images.
Principle
Current instruments are highly modified from the earliest versions, but the principle of confocal imaging that was developed by Marvin Minsky is employed in all modern confocal microscopes. The image in a confocal microscope is achieved by scanning one or more focused beams of light, usually from a laser or arc-discharge source, across the specimen. This point of illumination is brought to focus in the specimen by the objective lens and laterally scanned using some form of scanning device under computer control. The sequences of points of light from the specimen are detected by a photomultiplier tube (PMT) through a pinhole (or in some cases, a slit) and the output from the

5.1 Introduction
The process of milk processing entails the transformation of liquid milk into dairy products, including pasteurised liquid milk, yoghurt, butter, cheese, and ghee. The following are the rationales for processing: There are numerous benefits to processing milk, including the ability to access distant markets, the extension of the product’s storage life, and the attraction of higher prices because of its enhanced transportability. Furthermore, processed products are less cumbersome and lighter, which facilitates transportation. The refining of milk improves its quality and hygienic safety, enabling the production of a variety of milk products, including cheese and liquid milk, to satisfy the diverse needs of consumers. Additionally, the processing industry contributes to economic development by creating employment. Small-scale processors are capable of producing a wide range of dairy products. Before determining which dairy products the group will produce, it is recommended that a comprehensive market/feasibility study be conducted. Milk products can be processed in the manner depicted in the image.

Global climate change has increased the weather variability across all geographical settings. The transitional regions between the tropics and temperate region are expected to buffer the changes comparatively for a extended duration of time.

The techniques used in disease management mainly based on two principles,: Prevention or prophylaxis and cureThe first principle prevention includes disease management tactics applied before infection i.e., the plant is protected from disease. It includes: avoidance, exclusion, eradication, resistant&protection. Second principle curative action: functions with any measure applied after the plant is infectedi.e., the plant is treated for the disease.It includes therapy.

Characterization of Oct-4/Sox-2 Transcription Factors by Immunostaining for Characterization of Stem Cells
Protocol
1) The cultured cells at a density of 70,000/well in a 6 well plate or 35,000/ well in a 12 well plate are used for immunostaining.
2) The cells are washed with Dulbecco’s phosphate buffered saline (DPBS) for two times and the cells are fixed using 4% paraformaldehyde for 20 minutes at room temperature.
3) After fixation, the spent Paraformaldehyde is removed and the fixed cells are washed with 1000μl of 1x DPBS for 2 times by clockwise and anti-clockwise rotation of the plate slowly.
4) About 400μl of 3% blocking buffer was added to the wells and plate was covered with aluminum foil to prevent the exposure of light and stored at room temperature for 90 minutes.
5) After blocking, the cells are again washed once with DPBS. About 500 μl of primary antibody Oct-4 in the dilution of 1:40 is added to the well and stored at 4°C over night.
6) After overnight staining with primary antibody, the antibody solution is removed from the wells and washed with DPBS for 2 times.
7) Take 500 μl of secondary antibody anti-rabbit-FITC diluted with antibody buffer solution is added in the ratio of 1:200 and cover with aluminium foil and incubated for 90 minutes.
8) Secondary antibody is removed and washed with DPBS.

Object: Methods of measurement of water status and water saturation deficit in plant parts. 

Material required: Leaf samples, leaf borer, water, petridishes, forceps, oven and blotting paper. 

Theory: Plant water status strongly influences plant growth and biomass production through its effects on leaf and root expansion and on photosynthesis. Therefore, measurement of plant water status is an important component of understanding plant processes and maximizing yield under different ecosystems. 

Method: Plant water status can be measured and computed by any one of the following methods

Etiology

Demodecosis is caused by Demodex species follicular mite with increased numbers. But, it is generally caused by Demodex canis. It is divided into two forms – juvenile and adult forms. The juvenile form is more common. This condition may be localized or generalized in nature.

In recent years the terms "governance" and "good governance" are being used frequently in development literature. The concept of good governance is related to all branches of social sciences, especially, to political science, public administration, and economics. The term governance has become synonymous to sound development management. The concept of "governance" not new, it is as old as government itself. The term governance has become synonymous to sound development management. In recent times the concept of Good Governance first emerged in the mid-1980s as governability with the emphasis on adherence to the rule of law.

Background
Milk is known as a whole food for human consumption. It is the first food of the newly born human mammals. Milk contains almost all the nutrients required for the patients, the elderly, pregnant mother and new born baby. There is no doubt that milk plays a significant role in human development. We can prepare many physical forms of milk like cheese, yogurt, ghee, and milk powder. The calcium content is higher in buffalo than in cows. Buffaloes are the second largest source of milk and produce 15 % milk. Higher biological value proteins, fat, soluble vitamins, fatty acids, amino acids, water-soluble vitamins, and calcium are all present in milk. The main antioxidant components of milk and milk products are sulfur, phosphate, zinc, and amino acids like cysteine (necessary to produce collagen).
Milk somatic cells are normal cells found in milk in small amounts (epithelial cells), some of which are leukocytes (white blood cells). When mastitis is infected or injured, leukocyte production rises. Milk with a low SC level produces superior milk products that last longer. Better milk management, better cleanliness, and correct nutrition all contribute to a decrease in milk SC, which indicates poor milk production hygiene and a cause of mastitis. Milk somatic cells are made up of both immunological and milk-producing cells. In terms of defense, somatic cells are the second line. During the routine process of milking, these cells are secreted in milk. Somatic cell count is a good tool for evaluating farm cleanliness in bulk milk and is utilized worldwide as an indication of udder health in individual milk. The most prevalent condition affecting dairy buffalo production and their health and well-being is mastitis. The somatic cell count consists of immune cells (75%), such as neutrophils, macrophages, lymphocytes, erythrocytes, and epithelial cells (25%). Investigators have made different attempts to exploit milk somatic cells as a potential non-invasive sample to diagnose early pregnancy udder health in bovines.

In spite of the problems with dosage and viability of probiotic strains, a lot more work is required for industry standardization and safety issues. However, it is noted that probiotics have considerable potential against many pathogenic and other diseases. A wide range of clinical trials are still needed to standardize and optimize the conditions. Ongoing basic research will continue to identify and characterize existing strains of probiotics, identifying strain-specific outcomes, determine optimal doses needed for certain results and assess their stability through processing and digestion.

Defination

Dislocation of carpometarcarpus and tarso metatauses along with upper fracture of MC and MT bone (Fig. 1 and Fig.2). It is considered as difficult fracture and may not be healed appropriately by the usual procedure of lower level fracture repair method. Due to constant movement of fracture ends its treatment should be similar to middle level fracture repair method.

Materials Required

Thick padded cloth

Adhesive gum (fevicol)

Netted bamboo splint

Thomas splint

Principle: The copper in the Fehling solution is reduced to red, insoluble cuprous oxide in the presence of invert sugars like glucose and fructose. The quantity of sugars solution liquids for complete neutralization of a known quantity of Fehling mixture is determined by titration using methylene blue as the indicator.

(Lane and Eynon Method)

Government

Government is a territorial based body that makes authoritative decisions (for which it has constitutional or legislative authority) that are binding on residents and businesses within its boundaries. It is the executive arm of the state. Government refers to actions carried out within a formal legal setting; governance involves all activities of government along with formal activities even outside a formal governmental setting, which are meant to achieve common goals.

Governance is the process of governing through which decisions are made that are intended to affect societal outcomes, including economic, social, environmental and other important outcomes. It encompasses the direct and indirect roles of formal institutions of local government and government hierarchies, as well as the roles of informal norms, networks, community organizations, and neighborhood associations in pursuing collective actions.

Leucaena leucocephala, commonly known as Subabool or Koobabool, is a fast-growing leguminous shrub often used as fodder for livestock due to its high protein content and palatability. Despite its nutritional benefits, long-termor excessive feeding of Subabool can lead to adverse health effects, including dwarfism in livestock. This condition is primarily caused by the presence of toxic compounds in the plant, notably mimosine and its breakdown products, which interfere with normal growth and metabolic functions.
Nutritional profile of subabool
Subabool is a valuable fodder crop due to its high protein content (20–30%) and digestibility. It also contains significant levels of vitamins and minerals, making it a preferred choice for supplementing livestock diets, particularly in regions with limited forage availability. However, its nutritional benefits are overshadowed by the presence of anti-nutritional factors, particularly mimosine and its derivatives.
Mimosine and its toxic effects
Mimosine, a non-protein amino acid, is the primary toxic compound in Subabool. It constitutes about 5–12% of the dry weight of the plant and can exert toxic effects on animals through:

Background
Fine needle aspiration (FNA) is a minimally invasive diagnostic procedure used to obtain cells from a mass or lesion for examination under a microscope. It’s a valuable tool in diagnosing tumors in canines (Figure 5) and other species of animals, including humans.
Advantages of FNA
• Minimal invasiveness to the animals. Thus, no sedation of animals is required.
• Rapid results - Results are obtained faster as cell processing is not required.
• Cost-effective
• Risk-free
• FNA can be performed under image guidance, such as ultrasound, CT, and MRI, for precise identification of the location of cells inside the tissue.
• May be able to differentiate between benign and malignant tumors
• Can assess tumor grade
• FNA helps monitor treatment response. FNA is helpful as it can help pre-plan the treatment of the dogs. If the tumor is malignant, then the treatment can be given immediately, and if it is benign, then the treatment can be postponed.

Food extrusion technology is a popular means of preparing snacks and ready-to-prepare food items. It is a method of size enlargement in which tiny granular food or powdered particles are strengthened into bigger parts with various forms, texture, color, etc. Extrusion cooking or thermoplastic extrusion is a common extrusion technology considered to be a process of HTST (high temperature, short time). It allows the manufacturing of a wide range of food products with little or no alteration of the fundamental equipment and adequate process control. Extrusion cooking is used for starchy and protein substances to make nutritious foods. Generally, such products are rich in calories and incorporation of protein rich fish improves its nutritional value. Most of the cereal-based extruded snacks were primarily made from maize, wheat and rice. However, these cereals (e.g. rice) have comparatively low protein content (6 - 8 g/100 g db) and an elevated amino acid profile in glutamic and aspartic acid, whereas lysine is the restricting amino acid. Therefore, it is recommended that protein food ingredient (e.g. fish) be added to the extruded snack products to develop nutrient rich diets.

Fish is considered to be great sources of protein of high dietary importance with a balanced profile of essential amino acids and lipids containing omega-3 fatty acids, in particular essential fatty acids such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), respectively. EPA and DHA are very essential to normal growth and development. To avoid or moderate coronary artery disease, hypertension, diabetes, arthritis, other inflammatory and autoimmune illnesses, these vital fatty acids are discovered. The benefits of fish-based extruded products will assist in providing a big population in the nation like India with nutritious and balanced diets.