eChapters

3.1 INTRODUCTION

The chemistry of the soil is much more complex than can be represented simply by precipitation, adsorption, or exchange processes. The complexity arises from the time scale of soil chemical reactions in which the structure and dimensions, the extent and distribution of pores, and the content and mobility of water in them, interact immensely with the reaction chemistry. The fourth process adding to this complexity is the dissolution of native and reprecipitated soil constituents by percolating water containing solutes accumulated by natural processes or added by man. The fifth complicating factor compounding the time scale is that very often the resultants of soil chemical reactions are large complex molecules, occasionally polymeric, made up of several constituent groups or atoms. It is by no means clear which of the four processes participate in a chemical reaction and in which order. That a process reoccurs during a prolonged reaction is very likely.

Such complexities explain why no single model related rigorously to the retention and release of cations and anions, can provide a basis for a laboratory procedure to test and compare the chemical properties of soils. In this chapter, some of the soil reactions that are important to plant nutrition and pedological processes are discussed, principally those leading to relatively simple compounds accumulating in soil.

The term bioinformatics was coined by Paulien Hogeweg in 1979 for the study of informatics processes in biotic systems. It was primarily used in genetic and genomic studies, particularly in those areas of genomics involving large-scale DNA sequencing. Bioinformatics involves application of information technology to store, organise and analyse the vast amount of biological data available in the form of sequences and structures of proteins (the building blocks of organisms) and nucleic acids (the information carrier). There are umpteen ways to define bioinformatics, mainly owing to its rapid evolutions.

Abstract

The anaerobic fungi are one of the early organisms in evolution which are commonly found in the digestive tract of ruminating and non-ruminating animals, (including herbivores) deep sea and earth, dumping sites, etc. In animals, these are considered the primary colonizers of the plant biomass ingested by them. Still their taxonomy was unclear for a long period of time. However, with the help molecular techniques, they are now classified under a separate phylum Neocallimastigomycota. There are 38 species known so far belonging to 11 genera. These have been identified based on both morphological data (like thallus, rhizoids, flagella per zoospore, etc.) and phylogenetic analysis. There is a need to explore the undiscovered anaerobic fungi and look for their potential in industrial applications and solutions to the present environmental problems. It can be achieved by improving techniques of sampling, isolation, maintenance and long term preservation. Animals like herbivores do not have the ability to produce enzymes for degrading the plant biomass and anaerobic fungi help them by producing a range of extracellular enzymes. This ability of anaerobic fungi are of use to humans in bioenergy (biogas production) and biomass degradation (by producing cellulases). Recent advances in metagenomics coupled with new methods of cultivation are helping immensely to know the uncultivated anaerobic fungal populations from the animal gut. The present book chapter discusses the past and present of anaerobic fungi, lacunaes and need for more work on this group of fungi, It focuses on the progress in sampling, isolation and preservation techniques, simultaneously discussing the advances in the application these fungi in biotechnology.

Recombinant DNA Technology

A transgenic organism contains foreign DNA.

Biotechnology is the industry dedicated to using altered cells or molecules for various applications.

Recombinant DNA technology (i.e. gene cloning) was the first of the modern biotechnologies.

Gene targeting uses precision techniques to “knockout” a gene or substitute a gene for one on a chromosome.

Bioethics questions, gene patenting, and potential uses and risks of biotechnology pose treat questions for society.

Introduction

The Indian hot arid zone occupies an area of 0.32 million km to forming a continuous stretch in the north western states of Rajasthan, Gujarat, Punjab, Haryana and scattered land masses in the states of Maharashtra, Karnataka and Andhra Pradesh, 70 per cent of it falls in western Rajasthan. Low and erratic rainfall, extreme temperatures, long sunshine duration (6.6-10 hours), low relative humidity (30-80 per cent), high wind velocity (9-13 km/h) and high evapo transpiration (1600-1800 mm) and characteristic features of the region. Moreover the soils are poor in nutrients and scarcity of water and recurring droughts are perennial constraints. Despite hostile conditions, the western arid Rajasthan supports a large human and livestock population and a variety of flora and fauna. However, the ever increasing human and livestock population and development activities exert enormous pressure on the natural resource in the region. These areas experience an annual rainfall between 100 and 500 mm with a coefficient of variation varying from 40 to 70%. The region is characterized by low and erratic rainfall with extremes of temperature (1–48 °C), high wind velocity and sandy soils. In dry and arid region, wind erosion affects 13.5% in India (Sehgal et al., 1994, Kar et al.,1959). Wind erosion is very active in the Indian Thar Desert and poses severe multifaceted problems (Dey et al., 2016). Loss of nutrient-rich particles from agricultural fields has been occurring due to suspension of fine particles in air and deposition of eroded soil. Among terrain properties, soil aggregate distribution, surface roughness, soil moisture and vegetation cover are important factors influencing wind erosion. Indiscriminate grazing in the region also further destroys vegetation and exposes the land surface, thus making it more vulnerable to wind erosion.

1.    List of Spices
    
2.    Biotechnological Interventions in Spices
    
3.    Micropropagation
    
4.    Protocols for in vitro Multiplication of Some Spices
    
5.    Factors Affecting Large Scale Propagation Systems
    
6.    Field Performance of Tissue Culture Plants
    
7.    Other Tissue Culture Techniques
    
8.    Secondary Metabolites Production
    
9.    in vitro Germplasm Conservation
    
10.    Somaclonal Variation
    
11.    Molecular Marker Studies
    
12.    Disease Diagnostics
    
13.    Genetic Engineering
    
14.    Identification of Adulterants
    
15.    Future Prospects
        
References

The area of circle, rectangular, square, triangle, trapezium, rhombus,parallelogram etc are to be calculated by simple formulae but some times the combined area of more than two either circles or trapezium is difficult to calculate. It needs to understand the concept of area and how it is related to different planes. Some of the tips are given below to understand how to calculate the are

a. Area

A part of the plane enclosed by a simple closed figure is called a plane region and the measurement of plane region enclosed is called its area. Area is measured in square units.

The different geometrical shapes formula of area and perimeter with examples are discussed below:

4.1 Introduction

Many important traits such as yield, quality and some forms of disease resistance are controlled by many genes and are known as quantitative traits or characters (also called ‘metric’,‘polygenic,’ ‘multifactorial’ or ‘complex’ traits). The regions within genomes that contain genes associated with a particular quantitative trait are known as quantitative trait loci (QTLs). The identification of QTLs based only on conventional phenotypic evaluation is not possible. A major breakthrough in the characterization of quantitative traits that created opportunities to select for QTLs was initiated by the development of DNA (also called molecular) markers in the 1980s.

One of the main uses of DNA markers in agri-horticultural research has been in the construction of linkage maps for diverse crop species. Linkage maps have been utilised for identifying chromosomal regions that contain genes controlling simple traits (controlled by a single gene) and quantitative traits using QTL analysis. The process of constructing linkage maps and conducting QTL analysis–to identify genomic regions associated with traits–is known as QTL mapping (also called ‘genetic,’ ‘gene’ or ‘genome’ mapping). DNA markers that are tightly linked to agronomically important genes (called gene ‘tagging’) may be used as molecular tools for marker-assisted selection (MAS) in plant breeding. MAS involves using the presence/absence of a marker as a substitute for or to assist in phenotypic selection, in a way which may make it more efficient, effective, reliable and cost-effective compared to the more conventional plant breeding methods. The use of DNA markers in plant (and animal) breeding has opened a new realm in agriculture and horticulture called ‘molecular breeding’.

This guidance explains when a botanical drug may be marketed under an over-the-counter (OTC) drug monograph and when FDA approval of a new drug application (NDA) is needed. In addition, this document provides guidance to sponsors on submitting investigational new drug applications (INDs) for botanical drug products, including those botanical products (or botanicals) currently lawfully marketed as foods and dietary supplements in the United States. This guidance also discusses several areas in which, because of the unique nature of botanicals, FDA finds it appropriate to apply regulatory policies that differ from those applied to synthetic, semisynthetic, or otherwise highly purified or chemically modified drugs (including antibiotics). In particular, the guidance states that applicants may submit reduced documentation of preclinical safety and of chemistry, manufacturing, and controls (CMC) to support an IND for initial clinical studies of botanicals that have been legally marketed in the United States as dietary supplements or cosmetics without any known safety concerns.

A ratio is the comparison or simplified form of two quantities of the same kind. This relation indicates how many times one quantity is equal to the other; or in other words, ratio is a number, which expresses one quantity as a fraction of the other. For example, the ratio of 1 to 2 is 1 : 2. The numbers forming the ratio are called terms. The numerator, “1”, in this case, is known as the antecedent and the denominator, “2”, in this case, is known as the consequent.

In this article we will learn the approach applicable to solve various problems on ratios and proportions. In the next few lines you will go through some important concepts related to ratio problems and the methods you should apply to solve those problems. It is always better to solve as many problems as you can to boost your confidence. Some important points are given below to remember;

Key Facts

    •    The heavy metals (HM) or trace metals in an aquatic environment can come from natural (e.g. geological minerals, wind blown silicate dust, volcanic emissions, sea spray and combustion) and anthropogenic sources (e.g. mining, industry and sewage treatment discharges as well as electronic waste and agriculture fertilisers). In aquatic systems, the heavy metals of greatest concerns are cadmium, copper, lead, mercury and zinc.
    •    Metals may exist in a number of physico-chemical states in aqueous systems such as particulate matter and dissolved forms. Metal speciation in freshwater is strongly influenced by hardness, alkalinity, pH, natural dissolved organic matter (DOM) and redox potential. 
    •    Water hardness is quantitatively related to metal toxicity in freshwater (i.e. toxicity of heavy metals decrease with increasing hardness and alkalinity; on the other hand, toxicity increases with decreasing salinity, dissolved organic matter and chelators). The uptake and toxicity of heavy metals of most metals in marine waters is low as compared to freshwater because metals are easily complexed with chloride ions.
    •    The toxicity of HMs is related to the dissolved ionic form of the metal rather than total concentration of the metal. Each different physico-chemical forms of an element has a different toxicity, for example, if the element (e.g. Cu) is absorbed on a colloidal particles it is expected to be of little or no effect on aquatic organisms, however,  if the copper is available as free Cu (II) ion, few aquatic organisms may survive.

Energy is vital for the whole universe. Since the formation of the universe, energy acts a specific role. Steven Hawkins, George Ellis and Roger explained “The Theory of Relativity “Time and space had a finite beginning than corresponded to the origin of matter and energy from the time of BIG BANG.”. Before invention of fire, energy hovers everywhere but people did not have any knowledge about that. Living being requires energy for every day work which cells of the body provide them. Energy is also a basic requirement for the existence and development of human life. Energy plays an important role in the entire process of evolution, growth and survival of all living being and also in the social –economic development and human welfare of country. Energy is defined as the ability to work. Then we run or walk or do any type of work, we burn the energy in our body produced in cells by transforming food. Cars,planes,trolleys,ships, aeroplanes etc and all kind of machineries require energy to run. There are many sources to get energy as coal, natural oil, natural gas, wind, water, wave, sun and nuclear etc. The commercial sources of energy as fossil fuels (coal, oil, and natural gas) hydro-power and then nuclear power provide the need of energy now of the world. The demand of energy is growing an alarming rate. As for example, according to International Energy Agency the Global consumption of energy has increased from 4606 Mtoe (Million Ton Oil equivalent) in 1973 to 7287 Mtoe in 2003.

Suppose you go to a Big Bazar to buy a shirt. You finalize the shirt and are shocked to see its price tag. The price mentioned on the sweatshirt is Rs. 2500. You ask the owner to give some discount. He offers a discount of 20%. Now just figure out, how much you will pay to the shopkeeper. Did you say 2000 rupees? You are bang on1 You will pay a sum after subtracting 20% of amount written on price tag i.e. Rs. 500 from Rs. 2500. You finally pay the shopkeeper 2500 – 500 = Rs. 2000. This example introduces you to the concept of marked price and discount. What you indirectly learned from this case is that the discount is calculated on the marked price only. Now let us assume here that the shopkeeper has purchased that sweatshirt for Rs.1600. How much money that shopkeeper has earned by selling this sweatshirt ? Now the shopkeeper has invested Rs. 1600 to buy this sweatshirt and finally sells for Rs. 2000. He makes a profit of Rs. 2000 – 1600 = Rs. 400

He earns this profit by putting in Rs. 1600. Thus his percentage profit is 100 x 400/1600 = 25%. This makes another concept of cost price and profit clear to you i.e. the price you put in from your pocket to buy a product is called cost price and the extra amount you get after selling the product is called the profit. The profit and loss percentage is calculated on cost price. Profit and loss problems are an integral part of all the competitive exams. You must understand the meaning of the basic terms used in profit and loss questions.

This chapter focuses on the fundamental ways in which meteorological elements directly influence plant growth, development, and ultimately, agricultural productivity. Understanding these relationships is crucial for optimizing farming practices and mitigating weather-related risks. The growth and yield of any crop are profoundly influenced by the prevailing weather and climate conditions. Plants are biological systems highly sensitive to their surrounding environment, and meteorological factors dictate the rates of crucial physiological processes (Fig. 2.1).

2.2 Effect of Weather Parameters on Crops The effect of different weather parameters on crops is given below.

Small millets such as foxtail millet, proso millet, finger millet, barnyard millet, kodo millet, fonio millet, teff and little millet and pseudo cereals such as buckwheat,quinoa, amaranth and cockscomb and chia are the oldest crops and staple food for millions of people around the world. Small millets and pseudo cereals are the main source of fodder and feed material for livestock.

In most of the examinations, the questions related to boat and stream are asked. These questions are seemed to be very difficult but are very ticklish and easy to solve. The students need to concentrate on flow of stream upward and down ward. If the boat is moving towards the flow (downstream), the speed of boat and stream will be added and if the boat is moving towards opposite of stream flow (upstream), the distance covered by the baot will be equal to boat speed minus strean speed. Few important tips are given below to solve such type of questions.

Downstream and Upstream Motion

Downstream motion of a boat is its motion in the same direction as the flow of the river. Upstream motion is exactly the opposite. There are two parameters in these problems.

Words like rDNA (recombinant DNA) technology, cloning, genetically modified organism (GMO), living modified organism (LMO) and transgenics are of today’s common knowledge. However rather than scientific interest, debates on biosafety issues have made these terms more agnized among general people. Global, sectorial and individual protests against transgenic plants and animals are enunciated allover the world raising issues and concerns over risks and health hazards of using these organisms. Despite these protests and concerns, commercial use of transgenic organism as food, feed or other purposes are increasing at an exponential rate. Although there is not much scientific evidence where transgenics have caused havocs on ecological balance or human health, the biosafety issues need to be solved to provide a safer environment rather than be sorry and lament in the future for today’s mistakes. One of the major problems in supporting use of GMOs lies in the incapability of biosafety science to draw reliable and widely acceptable conclusions for labeling a safe tag on these organisms and their derivatives.