eChapters

Introduction

The most important factor that influences the activity of bacterial enzymes is temperature. Most ofthe organisms grow under mesophilic conditions (at25⁰C). If the optimum temperature is increased, the rate of growth of organism is affected due to changes in protein structure which results denaturation of critical cell protein and ultimately inhibitsthe growth. If temperature is below critical temperature, then there is complete stoppage of growth as less activation energy is available for critical process to occur. However, there are certain organisms which can tolerate extreme temperatures viz.

1. Psychrophiles: Tolerate and grow at > 20⁰C temperature
2. Mesophiles: Tolerate and grow at between 20⁰C and 45⁰C temperature
3. Thermophiles: Tolerate and grow at <45⁰C temperature.

The grasshoppers are widely distributed throughout the country and may be seen in abundance during monsoon season. For the generalized morphological description, this insect has been considered as the most suitable representative of class insects because its structural details are not much variable. Apart from this, being larger, it can be studied easily. The generalized insect body is divided into 3 distinct body regions: a head, a thorax and an abdomen. Grouping of body segments into distinct regions is known as Tagmosis and the body regions are called Tagmata.

The Head

Introduction

Most of the bacteria, excepting the photosynthetic bacteria, are damaged by ultraviolet (UV) radiation. UV rays have lower energy content and are capable of producing lethal effect in cells exposed to wavelengths in the range of 200 nm to 300 nm. Nucleic acids absorb these radiations leading to formation of thymine dimers which distort the structure of DNA. Distortion in DNA brings the interferences in DNA replication and transcription. The germicidal effect of UV can be used to determine the minimum amount of exposure required to produce 100% mortality of micro-organisms.

Requirements

1. Nutrient agar medium
2. Card board
3. Incubator
4. Petri plates
5. Inoculating loop
6. Bacterial cultures

Learning outcome

In this exercise we will learn about handling of:

1. 18-135 mm lens
2. 70-200 mm zoom lens
3. 8.3.5 mm DSLR lens
4. Babylight
5. Computer
6. DSLR camera
7. Field recorder
8. Mic rod
9. Power adaptor
10. Projector
11. SD card 32 GB
12. Speaker
13. Tripod
14. Projector screen

Requirements: Insecticides of different types

Insecticide chemicals in their “raw” or unformulated state are not usually suitable for pest control. These concentrated chemicals and active ingredients may not mix well with water, may be chemically unstable, and may be difficult to handle and transport. For these reasons, manufacturers add inert substances, such as clays and solvents, to improve application effectiveness, safety, handling, and storage. Inert ingredients do not possess Insecticidal activity and are added to serve as a carrier for the active ingredient. Manufacturers will list the percentage of inert ingredients in the formulation or designate them as “other ingredients” on their labels.

Requirements: Dissecting pan with wax pad, dissecting pins, forceps, hand lenses and scissors

Procedure

Place the specimen in the dissecting pan ventral side up. Use scissors to cut through the exoskeleton‘s ventral side from the head to the posterior end of the abdomen.

Pull the cut sides apart and observe the internal organs. Pin the sides of the insect to the dissecting pan. The observation of the reproductive systems of males and females are as follows:

Female reproductive system

The main functions of the female reproductive system are egg production and storage of male spermatozoa until the eggs are ready to be fertilized. The basic components of the female system are paired ovaries, which empty their mature oocytes (eggs) via the calyces (Calyx) into the lateral oviduct which unite to form the common (median) oviduct.

Materials Required 

Fruits/vegetables, hand refractometers, fruit pressure tester, weighing balance, scale, chemicals and glassware required for analysis.

Procedure

    1.    Go to the orchard or vegetable field and collect samples of fruits and vegetables
    2.    Apply the specific maturity indices to the samples

Insect collection is a source of recreation for many people and maybe a hobby for those who are interested in studying insects. Methods of collection and preservation of insects are the pre-requisite to studying the insects and their various internal and external organs. After collection, it becomes imperative to keep and preserve the insect specimens intact and safe for a longer time to further study the characters or to develop the insect collection museum. Let‘s have a look and do the different types methods of collection, and devices used for the collection and preservation of insects including immature stages in this practical session.

Collection

 The purposes of insect collection are:

To know the occurrence of a particular insect in a particular locality.

Taxonomic study of insects.

Identification of insects.

To study the bionomics and rearing of insects for various entomological experiments.

To develop the insect repository etc.

Sprayers

Sprayer is a machine used to apply liquid chemicals on plants to control pest and diseases. It can also be used to apply herbicides to control weeds and to spray micro nutrients to enhance plant growth. The main functions of a sprayer are

•             Breaking the chemical solution in to fine droplets of effective size.

 •            Distributing the droplets uniformly over the plants.

 •            Applying the chemicals with sufficient pressure for positive reaching the plants

 •            Regulating the amount of liquid applied on plants to avoid excessive application.

Learning outcomes

• You should know how to make an audio or video script.
• Describe the differences between the different kinds of programmes.
• Examine the different script development stages. Before we start, please complete the following activity.

Reflect on the programmes you watch on TV or any other programme using Over the top (OTT) platforms like amazon, prime video, Netflix etc. How many of it you classify as

1. Entertainment
2. Educational
3. Informational

The collected insect samples can be inspected in the laboratory after detaching the antenna and putting them under the microscope. They can also be studied through the permanent slides of different types of antennae with the help of microscope.

Antennae are mobile sensory segmented appendages of the head. They articulate with head in front or between the eyes and arise from antennal socket. The size and shape of antennae varies in different insects. They are used for sensory perception which includes motion and orientation, odor, sound, humidity, and a variety of chemical cues. The Sensilla on the antenna acts as tactile, olfaction, carbon dioxide, temperature, wind, humidity, and sound receptors.

Structure of Antenna: Antennae consist of three parts

Metamorphosis and immature stages

The change in growth and development (form) of an insect during its life cycle from birth to maturity is called metamorphosis. There are four basic types of metamorphosis in insects.

Ametabola: (No metamorphosis) e.g. Silver fish.

These insects have only 3 stages in their life cycle namely egg, young ones and adults. It is most primitive type metamorphosis. The hatching insects resemble the adult in all respects except for the size and called as juveniles. Moulting continues throughout the life.

Hemi-metabola: (Incomplete metamorphosis) e.g. Dragonfly, damselfly and may fly.

These insects also have 3 stages in their life namely egg, young ones and adults. The young ones are aquatic and are called naiads. They are different from adults in habits and habitat. They breathe by means of tracheal gills. In dragonfly naiad, the lower lip (labium) is called mask which is hinged and provided with hooks for capturing prey. After final moult, the insects have fully developed wings suited for aerial life

The complete system of veins of a wing is termed venation or neuration. Generally in all the insects, there is some similarity in wing venation and therefore, it is presumed that all types of wing venation have developed from the common base or the same ancestor. Using an extensive study of wing venation in different groups of insects, Comstock and Needham constructed a hypothetical type of wing venation from which all other types have presumably been derived. According to them the primitive wing venation has developed from two tracheae which are situated on the anterior and the posterior basal margins of wings and their branches are spread all over the wing.

Materials Required

Fruits/ vegetables, washing tanks/ containers, size grader, fruit waxes, packaging material, neem based fungicides etc. 

Procedure

    1.    Take available fruits or vegetables 
    2.    Wash them in plain water or chemical solution as told by the teacher
    3.    Shade dry the fruits / vegetables
    4.    Prepare suitable wax solution
    5.    Apply wax on to the fruits as told
    6.    Record the fruit weight before packing
    7.    Pack the fruits properly in the trays / boxes depending upon the type and purpose
    8.    Do curing if required 
    9.    Do pre-packaging, if desired
    10.    Label the package for desired information
    11.    Keep the fruits for storage
    12.    Record the observations pertaining to weight loss and rotting periodically depending upon the commodity and its shelf life.

The National Food Security Bill, 2011 designed to make access to food a legal right, is the last chance to convert Gandhiji’s vision of a hunger-free India into reality.

Mahatma Gandhi’s articulation of the role of food in a human being’s life in his speech at Noakhali, now in Bangladesh, in 1946 is the most powerful expression of the importance of making access to food a basic human right.  Gandhiji also wanted that the pathway to ending hunger should be opportunities for everyone to earn their daily bread, since the process of ending hunger should not lead to the erosion of human dignity.  Unfortunately, this message was forgotten after the country became independent in 1947, and government departments started referring to those being provided some form of social support as “beneficiaries”.  The designation “beneficiary” is also being applied to the women and men who toil for 8 hours in sun and rain under the Mahatma Gandhi National Rural Employment Guarantee Programme (MGNREGA).  Sixty-five years after Gandhiji’s Noakhali speech, we find that India is the home for the largest number of under- and malnourished children, women and men in the world.  There are more persons going to bed partially hungry now, than the entire population of India in 1947.

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Objective: To understand basics of photography and practice taking good quality photographs.

Overview: Photograph refers to any image which is created when light falls on a photosensitive surface. With the advent of mobile phones and decreasing cost of technology, many people are taking up photography as a hobby or profession. Photographs are very useful in communication, extension and education, as they arouse interest, simplify complex ideas/ things, can be used to convey messages to illiterate people, and enhance credibility of the information. Apart from technical expertise, it is important to know the basics of composition to take good photographs.

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Objective: To understand communication process and its elements.

Description: Communication is the process by which a person shares knowledge, feelings, ideas, and information, etc. with another person(s) in such a way that each gains a common understanding about the meaning and intent of the message. Major functions of communication include sharing information, issuing instructions, persuasion and motivation. There are several elements in communication process. Anyone who takes the initiative and starts the communication process is called the source or communicator. The intended recipient of the communication content is called as the receiver. Any sign or symbol which has the same meaning for the source and the receiver is called is the message. Receiver is the intended recipient of the message and what the communicator hopes to accomplish is the objective of communication process.

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Objective: To understand gap in communication process.

Description: Communication is never 100 percent efficient. In other words, even an ideal situation does not lead to complete and full understanding between the sender and receiver. Often, the receiver may understand the message differently or receive only a part of the message. This happens as every individual has a different frame of reference. Frame of reference is the set of experiences, beliefs and values which influence a person’s behaviour and acts as a benchmark against which all new stimuli are interpreted. Based on the frame of reference, every individual develops a perception. Perception is the process of receiving and interpreting stimuli.

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Objective: To understand shot sizes in a video production.

Overview: Shot, in video production refers to one continuous recording by the camera. It comprises of a series of frames (still images) and There are 24 frames in one second. A shot includes eeverything recorded between switching on and switching off the record button on the camera. It can contain movement of both camera and talent.

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Objective: To understand the characteristics and limitations of language.

Description: Language is the structured system of symbols, which are called as words. Semantics refers to the study of the relationship between symbols and their meaning. All languages are arbitrary as the word symbol is not the thing it represents (i.e. if all of us agree, we can refer to a “cat” as “donkey”, without turning it into one!). Words can be both abstract (things that cannot be sensed directly) and concrete (things that can be experienced through sensory organs).