eChapters

In dry combustion technique, the combustion of soil sample (about 250 mg and mm sieved) is carried out at high temperature (about 1000 ºC) through feeding in an inert boat in the electric furnace. The CO₂ produced from the oxidation of carbon present in the soil sample at high temperature is carried towards non dispersive infrared detector (NDIR) by a carrier gas where it is quantified and total carbon content is determined.

Phosphorus is nearly exclusive found as phosphates in waters and wastewater and it categorised in to: a) orthophosphate, (b) organically bound phosphate and (c) condensed phosphates. Theses all three forms of phosphorus occur in solution, in the body of aquatic organisms through food chain. All the forms of phosphorous, whether dissolved or particulate are converted to inorganic forms (phosphate) after digestion of sample. Various methods use for the digestion of the sample including use of perchloric acid, H2SO4- K2SO4, H2SO4- HNO3 etc. The H2SO4- HNO3 digestion technique is discussed here.
Reagents
1. Phenolphthalein indicator
2. Conc. sulphuric acid (H2SO4)
3. Conc. nitric acid (HNO3)
4. Sodium hydroxide (NaOH), 1 N: Dissolve take 40 g of NaOH in 1000 ml of distilled water
Procedure
Digestion
1. Take 50 ml of sample in a Kjeldahl flask
2. Add 1 ml H2SO4 and 5 ml HNO3
3. Once the HNO completely removed, digest the sample on a hot plate until the volume approaches 5 ml. Then, keep heating the solution until it loses colour (you may also heat it in a beaker covered with a watch glass to prevent excessive evaporation).

The total plate count (TPC) is also called the Bacterial plate count (BPC), aerobic plate count, standard plate count (SPC), total viable count (TVC) or mesophilic count represents the number of colony forming units (CFU) per g (or per mL) of growing microorganisms such as bacteria, yeast and mold on a nonspecific solid bacteriological growth medium under the specified conditions. Temperature, incubating time, medium type, and any other conditions used in the enumeration varies upon reference standards and laboratory objectives. Laboratories participating in the program choose their own routine conditions.
Principle
Usually direct count of too many bacteria in a sample is difficult but the bacterial colonies may be used as a measure of the bacterial cells in the known serially dilution and plated out on an agar surface in such a way that single isolated bacteria form visible isolated colonies. However, colony-forming unit might actually consist of a chain of bacteria rather than a single bacterium if the organism often develops various cell configurations, such chains. Furthermore, a portion of the bacteria might be grouped together. Thus, typically refer to the process of using the plate count technique as a means of estimating the number of colony forming units (CFUs) in that known solution. Then further this figure use to extrapolate the total amount of CFUs present in the original sample.

Objective: To estimate total protein in the plant sample by Folin-Lowry's method.

Background information:

Protein assay can be done to determine the level of protein in solution by the Folin-Lowry biochemical method. The concentration of protein leads to change in color of the solution and based on different color in different protein concentration solution the change in color may be digitized by colorimeter or spectrophotometer. This technique was developed by Oliver H. Lowry in the year 1940s. The tyrosine and trytophan amino acid residues in a protein sample produces purple or blue color in the absorption maxima at 660 nm by using Folin-Ciocalteau (having phosphate and sodium tungstate molybdate) chemical reagent. The color intensity of protein sample depends on the concentration of above aromatic amino acids (tyrosine and tryptophan), hence every sample will vary for color. For protein estimation the BSA (Bovine serum albumin) is widely used as standard protein molecule due to its cost effectiveness, purity and easy availability. This method of protein estimation is very sensitive and can detect up to 10 μg/ml. This is a relative method of protein estimation and may be interfere by salts, Tris buffer, nonionic & cationic detergents, EDTA, lipids and carbohydrates. For reproducible assay the incubation time, pH (9-10.5) is critical in this method (Lowry, et al. 1951).

Total Protein in Tissue Extract or Blood, Serum or Plasma
(Method of Folin-Ciocalteu, 1927 modified by Lowry et al., 1951)
Principle

A deep blue dye is formed when protein is treated with the phenol reagent of Folin ciocalteu. The extraordinary sensitivity of the method is due to the fact that two colour reactions are taking place simultaneously.
    a.    The biuret reaction of peptide bonds with copper in alkaline solution.
    b.    A reduction of phosphomolybdic acid and phosphotungstic acid by the aromatic amino acids, tyrosine and tryptophan, present in the protein.

Abstract
 
The definition of quality is described from a very simpler one “fitness for purpose or use” to a comprehensive “the totality of features and characteristics of a product or service that bear on its ability to satisfy stated or implied needs”. The concept of total quality management in food industry is adopted recently and it is the “integrated organization approach in delighting customer (both external and internal) by meeting their expectations on a continuous basis through everyone involved with the organization working on continuous improvement in all products/processes along with proper problem solving methodology”. TQM is concerned with the integration of all the efforts in organization towards quality improvement, quality development and quality maintenance to meet full customer satisfaction at economic levels. TQM enhances the work culture and employee’s satisfaction through participation and involvement and consequently the image of the organization.

INTRODUCTION

Library and information centers are primarily service institutions which are expected to provide information to the entire satisfaction of user groups. However, in recent times, it is observed that there is conglomeration of several services in a pin-pointed manner in which the libraries are facing problem of confusion. Therefore, leading library and information centers have started adopting emerging management principles and practices in their functions and operations. Among all these new traits of management the concept of quality management appears to have a dynamic impact upon the improvement of organizational objective and functions. Since agriculture libraries are vibrant information organizations their quality of services needs to be improved in the modern technological era. The application of Total Quality Management (TQM) is one of the vital aspects of the management techniques that appears to be very much applicable and can bring about a great transformation in quality of library services. The present paper makes an analytic discussion on the applicability of TQM in Agriculture Libraries in India.

11.1 Quality

• Quality of fruit or any other commodity is combination of attributes, properties or characteristics like appearance, texture, flavor and nutritive value that determine their value to the consumer.

• Keeping quality is also an important quality attributes in all fruits.

• Quality parameters • Appearance (size, shape, color and freedom from defects)

• Texture (firmness and juiciness)

• Flavors (swetness, sourness, bitterness, astringency, aroma and off f lavour)

• Nutritional (vitamins and minerals)

• Quality management Quality management is a method of ensuring the effectiveness of all the factors those implemented to improve and maintain the quality in acceptance form at consumer’s level.

11.2 Pre-harvest management of quality

11.2.1 Genotype and rootstock management

TQM is an integrative philosophy of management for continuously improving the quality of products and processes. It is used around the world.

TQM functions on the premise that the quality of products and processes is the responsibility of everyone who is involved with the creation or consumption of the products or services offered by an organization. In other words, TQM capitalizes on the involvement of management, workforce, suppliers, and even customers, in order to meet or exceed customer expectations. Considering the practices of TQM as discussed in six empirical studies, Cua, McKone, and Schroeder (2001) identified the nine common TQM practices as cross-functional product design, process management, supplier quality management, customer involvement, information and feedback, committed leadership, strategic planning, cross-functional training, and employee involvement.

TQM and Six Sigma

The Six Sigma management strategy originated in 1986 from Motorola’s drive towards reducing defects by minimizing variation in processes. The main difference between TQM and Six Sigma is the approach Six Sigma is a business management strategy originally developed by Motorola, USA in 1986. As of 2010, it is widely used in many sectors of industry, although its use is not without controversy.

Liberalized economies and evolving businessstrategies push variousindustries to achieve a competitive edge both in the domestic and international markets. Food industry especially, works on the bedrock of quality of products and services in order to attract and retain customers. In this new world of economic order, customers can select from wide range of products and services that are available in the market.

Objective: To isolate RNA from plants using TRIzol reagent and its quantification by spectrophotometer.

Background information

RNA (Ribonucleic acid) is a polymeric substance present in living cells and many viruses, consisting of a long single-stranded chain of phosphate and ribose units with the nitrogen bases adenine, guanine, cytosine, and uracil, which are bonded to the ribose sugar. RNA is necessary in all the steps of protein synthesis in all living cells and carries the genetic information for many viruses.

The isolation of RNA with high quality is a crucial step required to perform various molecular biology experiment. TRIzol Reagent is a ready-to-use reagent used for RNA isolation from cells and tissues (Chomczynski, 1993).TRIzol works by maintaining RNA integrity during tissue homogenization, while at the same time disrupting and breaking down cells and cell components. Addition of chloroform, after the centrifugation, separates the solution into aqueous and organic phases. Aqueous phase contains RNA.

After transferring the aqueous phase, RNA can be recovered by precipitation with isopropyl alcohol. DNA and proteins can be recovered by sequential separation after the removal of aqueous phase. Ethanol precipitation needs DNA from the interphase, and an additional precipitation with isopropyl alcohol requires proteins from the organic phase. Total RNA extracted by TRIzol Reagent is free from the contamination of protein and DNA The isolated RNA can be further used in Northern blot analysis, in vitro translation, poly (A) selection, RNase protection assay and molecular cloning.

Principle
The estimation of all solids, including dissolved, volatile, and suspended solids, is called total solids (TS). The residue that remains after the unfiltered sample evaporates between 103 and 105°C can be used to estimate total solids. Total dissolved solids (TDS) and Total suspended solids (TSS) are its two constituent components. After heating to 550°C, each fraction is separated once more into fixed solids and volatile suspended solids (VSS). The percentage of ash that remains is called fixed solids, while VSS is the organic proportion that was lost as CO2.
Apparatus
1. Ceramic or platinum evaporating dishes 100 mL volume.
2. Dessicator contain CuSO4.5H2O. Desiccator, provided with a desiccant containing a colour indicator of moisture (CuSO4.5H2O).
3. Analytical balance
4. Hot air oven
Procedure
1. Take a clean evaporating beaker or dish of required volume and dry it at hot air oven at 103 to 105oC for 1h, then cool and store it in a desiccator until needed. Immediately weigh before use. Note » the initial weight (Wi) in mg.
2. Take 250-300 ml or required volume of unfiltered well mixed sample in it (beaker or dish).
3. Place it in hot air oven at 103 to 105 oC for 2 h.
4. Cool it in a desiccator and take the final weight (Wf) in mg.
5. Replication the above process until a constant weight is achieved

Measurements of sugars in the fruit can indicate the stage of maturity. Total soluble solid (TSS) contents can give a reliable measure of the sugar and other water-soluble components in fruits, vegetables, and their products such as juice, squash, jam, preserve, candy, sauce, ketchup, etc., In climacteric fruits, carbohydrates accumulate during maturation in the form of starch, and as the fruit ripens the starch is broken down into sugar. In non-climacteric fruits, sugar tends to accumulate during maturation. The presence of sugars, acid, ascorbic acid, and other water-soluble components in the fruit, vegetable, and their products are measured as total soluble solids.
Principle
Soluble solids can be determined using a refractometer which measures the refractive index or a brix hydrometer which measures specific gravity. A Brix hydrometer is used to determine the percentage of brix in syrup while an Abbe refractometer gives both refractive index as well as degree brix. The Refractive index of the test solution is measured at 20°C using a refractometer and the soluble solid content is calculated by the use of tables correlating the refractive index with soluble solids.

Introduction

Banana plants are monocotyledonous, perennial and important crop in the tropical and sub tropical world regions. Traded plantain (Musa paradisiaca AAB) and other cooking bananas (Musa ABB) are almost entirely derived from AA-BB hybridization of M. acuminata (AA) and M. balbisiana (BB). Plantain and cooking bananas are very similar to unripe dessert bananas (M. cavendish AAA) in exterior appearance, although often larger. Main difference in the former is the starchy flesh rather than sweet and they are used unripe and require cooking. Dessert bananas are consumed usually as ripe fruits; whereas ripe and unripe plantain fruits are usually consumed boiled or fried. Large numbers of cultivars totalling about 70 are grown in different parts of the country. Some of the popular cultivars are Dwarf Cavendish, Robusta, Poovan, Nyali Poovan (Elakki Bale or Ney Poovan) Rasthali, Virupakshi, Monthan, Karpooravallli, Nendran or Rajeli. (www.pnbkrishi.com)

Commercial cultivation of Nendran has picked up rapidly in Tamil Nadu in the recent past. Nendran bunch has 5-6 hands weighing about 12-15 kg. Fruits have a distinct neck with thick green skin turning buff yellow on ripening. Fruits remain as starchy even on ripening. Keeping banana as a whole fruit for long time is not feasible due to its poor shelf life. The processed products from banana are also very limited at present and there is a good market potential for banana products such as banana figs, banana powder, banana jam, banana jelly and beverage (Federation of Indian Chambers of Commerce and Industry).

TVFA Estimation in Blood
(Method of Friedemann, 1938 modified by Mc Anally, 1944)

A method for the determination of volatile fatty acids in various biological materials has been described by Friedemann (1938) which is said to be applicable to blood, then the blood is added to dilute sulphuric acid, then sodium tungstate solution and solid magnesium sulphate are added and the whole is steam distilled.

Reagents
    1.    Saturated magnesium sulphate solution to which has been added 2.5 per cent by volume of concentrated sulphuric acid.

Touch Screen Kiosks
 
A touch screen is a device, which allows users to control a Personal Computer by simply touching the display screen by finger or pin. This type of input is suitable for a large number of computing applications. Most PC systems use a touch screen as easily as other input devices such as track balls or touch pads. If a touch screen computer system is installed at the kiosk it is known as touch screen kiosk. It may be Just like ATMs or modern plasma TV set which are developed as information kiosk and the information are made available to the users. A visitor can see the visuals and interact with kiosk to get the desired information. He can elicit the expert information by pressing keys or touching icons. Users can also follow the method of application of any technology through seeing of clippings at touch screen kiosk. In the touch screen kiosks, information is made accessible in the form of images, voice, video, graphics and texts. A large number of touch screen kiosks have been established by Indian railway, government offices, Banks and private companies where by touch of finger users can get access to various services (G2C and B2C) offered by the organization or company.

Introduction

India has a big challenge in fruit production in the current backdrop of decreasing per capita land holding and static fruit productivity. In recent years, China has surpassed and secured top position in the fruit production in the world accounting for 4,44,651 mt production followed by India and Brazil.  Now Chinese share in the fruit production is around 13 per cent while India contributes 10.2 per cent, Brazil 8 per cent and USA 6 per cent of total fruit production.

India is bestowed with a wide variety of agro-climatic conditions and enjoys an enviable position in the horticultural map of the world. Almost all types of fruit crops (tropical, sub-tropical and temperate) are grown in one or other part in the country.  The major tropical and sub-tropical fruits grown in India include mango, banana, papaya, orange, mosambi, guava, grape, pineapple, coconut, sapota, ber, pomegranate, litchi, aonla, bael, jackfruit etc.  Cashew nut cultivation has a big potential and its production, productivity and export has increased significantly in recent decades. Indian grape has recorded highest productivity per unit area in the world. The productivity of fruit per unit area in India has increased nearly from 10 t/ha to 12 t/ha in almost one decade.  However, the average productivity in most of the fruit crops is far below than satisfactory except grape and banana in few states.  It is paradox to record that India’s contribution in the overseas market is almost (0.11 per cent) negligible (Chadha, 2000). However, other countries like Mexico, Philippines and Venezuela, which produce far less, export 4 per cent of their total production.

Presently the farming situation urges need to develop farming techniques, which are sustainable from environmental, production, and socio-economic points of view. Modern agricultural production throughout the world does not appear to be sustainable in the long run. Sustainable agricultural development “is the management and conservation of the natural resource base and the orientation of technological and institutional change in such a manner to assure the attainment and continued satisfaction of human needs for the present and future generations”. Such sustainable development in the agriculture, forestry and fishery sectors, conserves land, water, plant and animal genetic resources, is environmentally non-degrading, technically appropriate, economically viable and socially acceptable. Such concerns imparted a way to organic farming. It is the need of the day to understand the prospects and problems of organic farming to launch a successful and flawless organic production programme in the farm of environment.

Organic farming

It is the most widely recognized alternative farming system to chemical agriculture evolved during 1940’s largely in response to the publication of J.I Rodale in the U.S., Lady Eve Balfour in England and Sir Albert Howard in India. In 1980, USDA released a landmark report on organic farming. The report defined organic farming as follows;

“Organic farming is a production system, which avoids or largely excludes the use of synthetically compounded fertilizers, pesticides, growth regulators, and livestock feed additives. To the maximum extent feasible, organic farming systems rely upon crop rotations, crop residues, animal manures, legumes, green manures, off-farm organic wastes, mechanical cultivation, mineral bearing rocks, and aspects of biological pest control to maintain soil productivity and tilth to supply plant nutrients, and to control insects, weeds, and other pests”.

In 1920, although electric vehicles were not used on the American farms about 5,000 electric industrial trucks were produced by 12 American manufacturers, while estimates of the total fleet ranged from 6,000 to 15,000, most of them built during the war. Most used an exchange charging system. Among the manufacturers seen in and around cities and towns were the Commercial Truck Company, the Lansden Company, the Walker Vehicle Company (now owned by Commonwealth Edison), the Ward Motor Vehicle Company, the Steinmetz Electric Motor Corporation, Walter Motor Truck Company, and the Baker R L Corporation.One step away from agriculture, Charles A. Ward of New York City, was head of his family’s baking company, made up of the Mackey Company, the Ward Corby Company, the Ward Bread Companyand the Ohio Baking Company of Cleveland. In 1911, Ward had decided to replace his horse-drawn delivery vehicles with electric trucks, thus doing away with the unsanitary stable hitherto part of or adjacent to bakery.¹

Alongside this, the challenge of using stationary electric agricultural machines continued to be met. Two innovations which entered into dairy farming were the milk cream electric centrifugal separator and the surge milker. The Babson brothers (Henry, Fred and Gus) of Chicago were friends of and dealers for electrical battery inventor and entrepreneur Thomas Edison, and importers of Belgian-made Méllote separators. In 1916, the Babsonsproduced their own Pine Tree milking machine. While the overall design was similar to others, the electrically powered Pine Tree Pulsator outperformed any other pulsator made. These machines were powered by a 32-volt Delco system with 12 batteries near the barn. Pine Tree Pulsators were simple enough that the farmer could maintain them himself. The Pine Tree Pulsator was patented in 1921. The problem with this unit was that it was difficult to keep clean.

Livestock and livelihoods are intimately linked in our country.  Mixed farming involving crop-livestock as well as sometimes fish integration has been both a way of life and a means to sustainable livelihoods.  Also, the ownership of the livestock is more egalitarian than that of land.  That is why in our ancient culture, livestock has been given a pride of place in the area of spiritual veneration and natural resources conservation.  Maharashtra is rich in its livestock resources and has also a long coastline with a wide range of living aquatic resources.

The crop-livestock integrated farming system provides an opportunity for promoting organic farming and restoration of soil health, and thereby of an ever-green revolution leading to an enhancement of productivity in perpetuity without associated ecological harm.  It also strengthens household nutrition security.  The country as a whole has over 500 million farm animals.  However, grazing land has been reduced to less than 5 per cent of the arable land area.  Village common property resources, which used to provide fodder and shelter to farm animals, have been overexploited.  Consequently, our farm animal productivity is very low.