eChapters

Abstract

Real-time PCR, also known as qPCR, is a highly sensitive technique used to determine the real-time amplification of a target DNA sequence. However, the presence of secondary structures or spurious amplicons can distort the results. When using SYBR Green chemistry, the features of qPCR primers differ from those of standard endpoint PCR primers. As a result, designing primers for SYBR Green qPCR requires extra caution and attention. This chapter outlines the essential features and conditions for designing qPCR primers, including primer length, melting temperature, GC content, specificity, and avoiding primer-dimer formation.

a) Transformation towards Commercial Agro: The poultry sector in India has experienced a revolutionary shift in structure and operation, which has led its transformation from a simple backyard activity to a major commercial agro based industry over a period of decades.
b) Layer Farming: The production of eggs has increased from 1.83 billion numbers in 1950-51 to 103.32 billion numbers in year 2018-19. The per capita availability reached at 79 eggs per annum in the year 2018-19 from 5 eggs per annum in year 1950-51. Andhra Pradesh, Tami Nadu and Telangana contributes about 55 percent share in total egg production.
c) Broiler Farming: Chicken meat contributes to 50 percent share in total meat production in year 2018-19 with the production of 4.06 million tonnes production. Broiler production is mainly concentrated in
Maharashtra, Haryana, West Bengal, Tamil Nadu and Andhra Pradesh. Broiler farming is mainly done through contract broiler farming. In that the integrator provides inputs such as chicks, feed, medicines and
vaccines. The contract farmer provides labour, shed, electricity, water, litter material, and other miscellaneous services or equipment that may be required. The integrator bears the transportation cost, investment and marketing risks. 

1.    Kernel type: Dent, Flint, Floury, Waxy, Sweet and Pop corn 
        •    Dent corn (Zea mays indentata Staurt.)
        •    Flint corn (Zea mays indurate Staurt.)
        •    Pop corn (Zea mays everta Staurt.)
        •    Sweet corn (Zea mays saccharata Staurt.)
        •    Flour/soft corn (Zea mays amylacea Staurt.)
        •    Waxy corn (Zea mays ceatina Kulesh)

Objective: To study the seed germination, pollination and dispersal type of various temperate tree species.

Observation recording: To record the observation on seed germination, Pollination and dispersal needs to conduct frequent field visits in different seasons for recording the various pollination and dispersing agents and conducting experiment on seed germination.

Modern agriculture faces unprecedented challenges due to climate change, unsustainable farming practices, and declining soil health. In response, nanoparticle-bioinoculants (nano-bioinoculants) have emerged as a promising innovation that integrates nanotechnology with microbial inoculants to enhance plant productivity and sustainability. Conventional agriculture contributes significantly to greenhouse gas emissions, degrades microbial diversity, and compromises ecological balance. Beneficial microbes, known as bioinoculants, play a vital role in improving plant health through nutrient cycling, hormone production, and disease suppression. However, their field performance is often limited by environmental instability and low survival rates. Nanotechnology provides a solution by enabling the encapsulation of bioinoculants within nanoparticles, enhancing their stability, targeted delivery, and effectiveness. These nano-scale carriers exhibit high surface-area-to-volume ratios, improved reactivity, and strong adhesion properties, making them ideal for enhancing microbial colonization and activity in the rhizosphere. Additionally, nanoparticles function as nano-fertilizers and nano-pesticides, supporting both plant nutrition and protection against pathogens. Plant-based nanoparticle synthesis further offers an eco-friendly alternative, minimizing ecological and health risks. Studies demonstrated that nano-bioinoculants improve seed germination, stress resilience, photosynthetic activity, and crop yield across various species. They also mitigate abiotic stresses such as drought, salinity, and heavy metal toxicity while promoting systemic resistance against biotic threats. The synergistic combination of nanoparticles and microbial agents enables multifunctional benefits—supporting soil health, reducing chemical dependency, and enhancing agricultural resilience. While challenges remain in understanding

Maize is a tall, determinate annual C₄ plant varying in height from (1-4) meters producing large, narrow, opposing leaves (about a tenth as wide as they are long), borne alternately along the length of a solid stem. The botanical features of various plant parts are as follows: 

Root

Root system of maize consists of seminal roots, crown or coronal or adventitious or fibrous roots and brace, pop or aerial roots. Seminal roots - which develop from radical and persist for long period. Crown roots developing from the lower nodes of stem below ground level which are the effective and active roots of plant and.  Brace or prop roots, produced by lower two nodes. The roots grow very rapidly and almost equally outwards and downwards. Favorable soils may allow corn root growth up to 60 cm laterally and in depth.

Vacuole: A fluid filled space within the cytoplasm surrounded by membrane called as tonoplast, that occupies most of the volume of cell. Aside from storage, the main role of the central vacuole is to maintain turgor pressure against the cell wall.

Valinomycin: An antibiotic that uncouples electron transport and oxidative phosphorylation only in presence of some cations such as K⁺ or Na⁺

Van der Waals interaction: Weak intermolecular forces between molecules as a result of each inducing polarization in the other.

Papaya (Carica papaya) is a important delicious fruits of herbaceous in nature,. It is most precocious fruit crops cultivated as pure as well as intercropped with mango, guava, peach, and coconut. Papaya starts bearing in 8-10 months after planting.

Observation recording: Today green felling is completely banned. Fuelwood species are managed under clear-felling systems and tree species infected by insects and pests are felled selectively. The students are advised to visit the fuelwood plantations and forests areas where selective felling is going on.

Abstract

FASTA, which stands for “Fast All”, is a sequence alignment tool developed by William R. Pearson and David J. Lipman in 1985. It is commonly used to identify similarities between DNA and protein sequences. FASTA employs a “hashing” strategy to locate matches for short stretches of identical residues with a length of k, referred to as k-tuples or k-tups. This method enables FASTA to quickly search large databases for sequence similarities, making it a popular tool for identifying homologous sequences, predicting gene functions, and analyzing evolutionary relationships. This chapter elaborates some basics of FASTA.

“Curriculum” is seen often as a critical issue during discussions on quality of agricultural education, being considered variously as content, as product and as process. It is easy to make the assumption that a common understanding of curriculum, but in practice this is far from the case, with a host of definitions, approaches and ideological standpoints. Even though a dialogue on curriculum theory is well established, there is still a strong tendency in practice to understand curriculum as a list of content, often developed in considerable detail, that students should be taught.

Cloud usually consists of very small droplets of water which cannot fall appreciably below the cloud base without evaporation. In this condition clouds are stable. Rain drops are about a million times heavier than cloud drops and rain develops only if the cloud droplets grow by some mechanism. Cloud Seeding mechanism depends on the difference in vapour pressure over ice and super cooled cloud, water evaporates of the droplets and migrate to the ice crystal, which grows and begins to fall increasing their growth rate by coalescence with other droplets and eventually melting and falling as rain drops. Presence of ice nuclei determines whether ice crystals form in a supercoold cloud or not. Ice crystals form on naturally occurring nuclei at temperatures of – 10⁰ to 30⁰ C. At warmer temperatures, either the efficient ice-forming nuclei are often deficient in the atmosphere or the cloud may occur with tops supercooled but not to an extent which activates the ice nuclei available. It should be possible to initiate precipitation by introducing ice crystals or by supplying artificial nuclei.

Fig. 25: ICPH 2671 in field

Answer the followings
    Q.1.    What is the different stage of pathogenesis in mastitis?
    Ans.    There are three stages in pathogenesis 
These are as follows:
Invasion: here pathogens move from the exterior of teat to milk inside the teat canal.
Infection: Here pathogens multiply rapidly and invade the mammary gland.
Inflammation: In this stage clinical mastitis occurs with varying degree of clinical abnormalities of milk and udder.
    Q.2.    Of these phases, which phase is important to reduce the incidence of mastitis in control program?
    Ans.    Invasive stage is very important. The incidence of mastitis can be reduced by taking preventive measures at invasive stage by good management and good hygiene practices.
    Q.3.    What are the common abnormalities encountered in udder during mastitis?

Antibiotics are substances which are produced by micro-organisms and which act against micro-organisms. It is also defined as secondary metabolites produced by microorganism, which inhibits the growth of another microorganism. Most antibiotics known uptill now are products of actinomycetes and some are from fungi and bacteria. The use of the antibiotics in managing plant diseases, particlulaty the disease caused by bacteria, mycoplasma and ricketsia, is well demonstrated and are thought to be effective means because of their selective action directed towards causal agents not to the infected host. The mode of action of the antibiotics varies depending on site of action which in turn detrmines their specificity towards given target host (Table 12). Some time mixture of antibiotics are in practice in managing the diseases to reduce delay the chances of resistance development against a particular antibiotic by the causal agent. The most useful antibiotic mixture is streptocycline, which contains streptomycin and tetracycline.

Wall pressure: The inward pressure exerted by the cell wall against expanding protoplast; equal but opposite to turgor pressure.

Warburg effect: Warburg effect states that the competition between CO₂ and O₂ for enzyme RuBisCO with CO₂ promoting photosynthesis and inhibiting glycolate formation and inhibiting photorespiration and O₂ promoting glycolate formation and inhibiting photosynthesis.

Water deficit: Water available to the plant is less than optimum requirement.

Water potential (ψ_w): Water potential is defined as free energy status of water in a system compared to the free energy status of pure water. Here system refers to water in plant cell or soil and pure water refers to the water which does not have any solute in it. Water potential is measured in terms of bars, Pascals or Megapascals and denoted by ψ_w.