eChapters

Abstract

Synthetic biology is a multidisciplinary field of study that seeks to understand living systems and organisms, and utilizes engineering principles to design and create new biological components, devices, and systems, as well as modify existing natural systems. This chapter provides an introduction to synthetic biology and describes the procedures and methodologies involved in the design and construction of novel biological systems. The applications of synthetic biology are vast and include the development of biosensors, gene circuits, metabolic pathways, and genetically modified organisms for use in a variety of industries, including healthcare, agriculture, energy, and environmental remediation.

Introduction
Project title:
Objective (S):

    Q.1.    How many species of Mycoplasma are responsible for causing mastitis?
    Ans.    At least seven species like Mycoplasma bovis, Mycoplasma Canadensis, Mycoplasma bovigenitalium, Mycoplasma alkalensis, Mycoplasma capricolium, Mycoplasma californicum, Mycoplasma disper and Ureaplasma sp. are responsible for causing mastitis

    Q.2.    Which herds are more prone to such type of mastitis?
    Ans.    The herds where milking hygiene is very poor and when the cows are imported from other farms or from public sale yards.

    Q.3.    Which organs act as common habitat of the Mycoplasma organisms?

Purpose

To make a list on the requisites on materials required for preparation of rehabilitation mobile sling for large animals.

Heart

The heart is a cone shaped thick walled and four chambered muscular organ. It lies obliquely craniocaudal in the middle mediastinum in between the left and right lungs and is covered by pericardium inside the thoracic cavity. The major part of heart (about 60 per cent) lies to the left of the middle mediastinum. The broad upper end is the base of the heart. It lies at the junction of the dorsal and middle third of the dorsoventral diameter of thorax at the level of about third to sixth rib and it faces craniodorsally. The narrow lower end is the apex. It lies just dorsal to the sternum at the level of about sixth rib and it faces caudoventrally. The surfaces, right and left are convex. They meet cranially at the right ventricular border and caudally at the left ventricular border. The right (cranial) border is strongly convex. It runs ventrally and caudally. The greater part of this border lies parallel to the sternum. The left (caudal) border is much shorter and nearly vertical. The surfaces of the heart are marked by grooves which indicate the division of the heart into four chambers the two atria dorsally and the two ventricles ventrally. The atria are separated internally by the interatrial septum. The ventricles are separated by interventricular septum. The circular coronary groove indicates the division of the heart in to atria dorsally and ventricles ventrally. It encircles the heart in its upper third part except at the level of the origin of pulmonary artery. The coronary groove is quite deep and wide. It provides passage for the coronary artery and vein. Apart from these, it is deeply filled with fat. The right (subsinuosal) interventricular groove and left (paraconal) interventricular groove indicates the line of separation between the two (right and left) ventricles by the interventricular septum. Both of these grooves run downward from the coronary groove towards the apex parallel to the respective borders and terminate just above the apex. The right and left ventricular grooves are occupied by the respective coronary vessels along with the fat.

3.1. Conventional farming

Refers to the use of synthetic chemical fertilizers, pesticides, herbicides and other continual inputs, genetically modified organisms, heavy irrigation, intensive tillage and concentrated monoculture production.

Highly resource and energy intensive, but also highly productive.

Conventional farming typically involves monocropping, but very expensive.

A ‘spice’ is a seed, fruit, root, bark, bulb, corm, rhizome or any other part of a plant primarily used for enhancing the taste, flavor or color of food. Some spices serve as preservative of food substances. Many spices have antimicrobial properties. Spices are sometimes used in medicines, religious rituals, cosmetics, perfumes etc. Spices are more commonly used in countries having warmer climates, especially in meat preparations. Many spices have substantial antioxidant property due to the presence of phenol compounds, particularly flavanoids.

India is the major producer of spices and contributes about 75 per cent of world’s spice production. Developing and under developed countries of the world supply about 55 per cent of spices to the global markets and the major markets in global spice trade are the United States of America, The European Union, Japan, Singapore, Saudi Arabia and Malaysia.

Leaves in the centres appear bleached and may have brown edges. Shade should be provided to reduce the light level to 1800-2500-foot candles to control this problem.

Observation recording: Students are advised to visit the Mahogany plantation for collection of seeds/stem cuttings then raised in polythene bags and record the various nursery practices and conduct tending operations in Mahogany plantation.

Mountains play a vital role in sustaining about 10 per cent of the world population directly as they are the major source of water supply and majority of rivers originate from these ecosystems thus sustain the life of people residing in the plains. In India hill and mountainous areas are vastly distributed all over the country with the major mountain ranges in the Himalayas and the Western Ghats. The Himalayas are extending in 2500 km length and 250 to 400 km breadth (Khanday et al. 2004). They are further classified in three major categories i.e Western Himalayas (including Jammu and Kashmir and Himachal Pradesh), Central Himalayas (including eight hill districts of Uttarakhand), North Eastern Himalayas (including Sikkim, Manipur, Meghalaya, Nagaland, Tripura, Union Territory of Arunachal Pradesh, Mizoram, hill areas of Assam and Darjeeling district of West Bengal). Whereas Western Ghats also known as Sahyadri including border of Gujarat, Maharashtra, Goa, Karnataka, Kerala and Tamil Nadu .

The motive behind conducting the workshop on Capacity Need Assessment and Development is that the existing Extension Advisory Services (EAS) needs new capacities to respond effectively to the emerging challenges in agricultural development.

Leptospirosis

Leptospirosis is an infectious disease of animals caused by spirocheate Leptospria sp. and characterized by widespread haemorrhage in visceral organs, nephritis, icterus, hepatitis and anemia (Fig.6.1).

Etiology

• Leptospira ictehaemorrhagae

• L. pomona

Pathogenesis

The organism is transmitted through direct contact with urine of infected animals or ingestion of urine contaminated feed or water. It can also be transmitted through aborted fetuses and infected uterine discharges. The organism enters through abraded skin and mucous membrane and reaches in blood and multiplies rapidly in blood producing septicemia and high rise in body temperature. Thereafter the organism localizes in the kidneys leading to acute or chronic nephritis. In kidneys, it multiplies in proximal convoluted tubules (PCT) and is excreted in urine which acts as source of infection.

Reducing insecticide application to the lowest level is the motto of IPM in vegetables. Monitoring for the initial build up and timely mechanical removal of the first population is a must in vegetables. Always remove the first affected plants, by diseases like mosaic, and destroy them by burying or burning. Remove insect eggs, insect affected plant parts and adults and immature stages of insects, and destroy them every day. Do not throw them in the field. They will either come back, or pupate and attack later. During nursery stage, use wood ash, cow dung solution, and plant extracts to deter all pests. Use only low residual insecticides like Dichlorvos 1ml/l, Malathion 2ml/l, Endosulfan 2ml/l or Quinalphos 2ml/l, in spots, at the beginning of attack itself.

Q₁₀value: The number of times a reaction rate change with a 10^oC change in temperature is called Q ₁₀value. If reaction rate doubles with 10^oC change in temperature, Q ₁₀= 2. It is also called temperature quotient.

Q-enzyme: It is a branching enzyme responsible for formation of 1-6 bonds in amylopectin.

QHV process: It stands for Quality Has Value Process.

QO₂ : It is a unit for measurement of respiration. Or It is the oxygen uptake in microlitres per milligram on dry weight per hour.

Quailing: In this practice the oranges are kept in the baskets in which they are harvested to allow the fruit to loose little moisture. It is done for a couple of hours before transport to packhouse. This practice would allow the fruit to lose a little moisture which is called quailing. This operation is done for oranges to prevent Oleocellosis.

Qualitative measurement: It is a subjective / objective measurement of the quality of the product for presence of certain compound. It gives the idea of the presence or absence of the particular compound. It deals with the nature of the substance and not its amount or quantity.

Quality: Quality is a measure of the degree of excellence or degree of acceptability by the consumer. The quality may also be defined in term of end use and may vary depending upon consumer’s perception and need. Or quality is the totality of features and characteristics of a product that bear on its ability to satisfy stated or implied needs. It is a term defined by the consumer, buyer, grader, or any other client based on subjective and objective measurements of the food product.

It consists of 2 parts-A) fabrication of fixed supporting frame B) fabrication of animal swing frame.

Fabrication of fixed supporting frame -

Materials required - Bamboo pole and rope

Fabrication Technique - Size a frame 3m for large animals, 2.5m for medium sized and 2 ml for small sized animals.

Fabrication of animal swing frame-

Materials required - Strong fixed rectangular iron frame

Fabrication technique - The required no. of bicycle tyres are fastened upon the frame. All individual tyres are tied with jute threads at 3-4 places.

The great vessels that leave and enter the chambers of the heart include the Aorta, the Pulmonary Artery and the Pulmonary Veins.

Aorta

The Aorta is the main systemic arterial trunk. It begins as ascending aorta at the base of the left ventricle, passes craniad and dorsad between the pulmonary artery on the left and the right atrium on the right and then turns caudad inclining slightly to the left, thus describing a curve called the aortic arch with its convexity directed upward and forward and the concavity facing downward and backward. From here it proceeds caudad on the ventrolateral surface of the thoracic vertebrae and the longus colli muscle as the descending aorta. At its origin from the base of left ventricle, the caliber of the aorta is greatest forming the bulbus aorta and here it presents three dilatations called the aortic sinuses. As the descending aorta reaches the level of the seventh thoracic vertebra it continues behind as thoracic aorta running through the middle and caudal mediastinum between the left and right lungs. The thoracic aorta first lies to the left of the median plane and then gradually inclines inwards medially and becomes median in position when it reaches to the diaphragm. It then passes through the hiatus aorticus of the diaphragm and enter the abdominal cavity as abdominal aorta. From here it passes caudad below the bodies of the lumbar vertebrae and the psoas major muscle. After reaching at the level of the sixth lumbar vertebra, the abdominal aorta terminates in the iliac quadrification by dividing into two external and two internal iliac arteries.

1. Botanical Name : Pyrus pashia L.

2. Family : Rosaceae

3. Common Name : Himalayan pear, Indian wild pear, Wild pear

4. Name in Indian languages

Kainth, Chotia, Kaimh, Shegal (Himachali), Mehal, mol (Hindi), Mehal, mol (Kumaoni), Mayal, Passi (Nepali), Kainth, Shegal (Punjabi), Sohshur (Khasi), Soh ait-syiar (Pnar), Tangi (Kashmiri).

5. Description

Himalayan pear is a small, deciduous tree of rosaceae family. It is common in open field borders and forest margins from 1000-2400m. The tree reaches up to a height of 5-8 m. The stem is greyish brown and rough. The flowering takes place in the month of February-March and the fruits mature in month of October to November. Fruits are globose, brown ultimately becoming black and rough. The fruits are astringent in taste when immature. The ripe fruits are sour -sweet with some astringency. This is widely used as rootstock for pear.

Gladiolus is a fluoride pollution indicator plant, as it expresses the effects of fluorine at very low levels. Symptoms of scorching of the leaves look like drying of the tips of the leaves. A strong application of super phosphate is also associated with fluoride toxicity. By spraying 5 percent lime or Magnesium sulphate, it decreases