eChapters

1.    Milking tube : It is used for milking the animal after udder or teat surgery. It can also be used for infusing intramammary medication through teat canal.
 
 2.    Teat plug : It is used to plug the teat in cases of leaky teats or trodden teats or following operation at teat sphincter. It can also be used for retaining medication in the teat canal.
 
 3.    Udder infusion tube : It is used for infusion of medication in the udder/teat.

 4.    Teat slitter with concealed blade : For cutting any growth inside the teat canal without injury to the teat.
 
 5.    Teat tumor extractor : It is used for removal of teat tumour or polyps from the teat canal. 

Xanthan gum: It is a polysaccharide that is produced by fermentation of carbohydrates by a gramnegative bacterium (Xanthomonas campestris) and is a thickening and suspending agent used in pharmaceuticals and prepared foods. It is also called xanthan. Xanthan is a stabilizer produced by Xanthomonascompestris(Plate 257). Xanthan is not hydrolysed or degraded by humans.

Synthesis of Zn Oxide Nanoparticle

Aim

To synthesize Zn oxide nanoparticle

Introduction

Zinc oxide nanoparticle are effective anti-bacterial and antiodur agent. They can be synthesized by number of techniques including hydrothermal methods. Their synthesis using solvothermal process is described, (Aneesh et al., 2007).

1. Botanical Name : Salacia chinensis L.

2. Family : Celastraceae

3. Common Name : Chinese salacia

4. Name in Indian languages

Chourondi (Malayalam), Saptachakra (Sanskrit), Chuntan, Karukkuvai (Tamil).

5. Description

It is a usually climbing plant, though is sometimes a scrambling shrub and occasionally becomes more tree-like. It can grow from 3 - 10 metres tall with a stem up to 16cm in diameter. The plant is harvested from the wild for local use as a food and medicine.

6. Origin and distribution

It is originated in coastal India, China, Sri Lanka, Myanmar, Indonesia, Cambodia, Laos, Vietnam, Malaysia, Indonesia, Philippines. It is distributed in the Indian subcontinent, including the semi-evergreen forests of the Coastal Karnataka, Kerala and Tamilnadu.

Introduction

The international monetary system refers to the operating system of the f inancial environment, which consists of financial institutions, multinational corporations, and investors. It encompasses a set of regulations and norms designed to facilitate global trade and facilitate the movement of capital and investment between nations. It involves a network of government and f inancial institutions that establish exchange rates for different currencies. The evolution of this system is remarkable, considering its origins centuries ago when currency relied on gold coins and people engaged in bartering goods for trade. It's incredible how far the financial system has come from the days centuries ago, when the only form of money was gold coins, and transactions required bartering of goods or products for cash. The international monetary system provides financial support to nations in order to help them overcome debt and poverty. Inflation is a phenomenon that occurs when there is an excess of supply relative to demand.

Evolution of the International Monetary System

Prior to 1870, there was a bimetallic international monetary system, in which coins made of both gold and silver could be used as means of exchange. Each currency's gold or silver content governed the exchange rates between them. There were certain nations that used the gold or silver standard.

Yabby: It is one of the burrowing Australian crayfishes (Cherax destructor) that are used for food.

Yakitori: A bite-size marinated pieces of beef, seafood, or chicken on skewers is called yakitori.

Yam: It is edible starchy tuberous root of plants (genus Dioscorea of the family Dioscoreaceae) used as a staple food in tropical areas.

Yautia: It is one of the aroid plants (Xanthosoma sagittifolium) chiefly of tropical America with starchy edible shaggy brown tubers that are cooked and eaten like yams or potatoes.

Yearling mutton: Meat obtained from 12-20 month aged sheep. It is also called Earling mutton.

Yeast: Unicellular plant body, very minute in size containing cell wall, cytoplasm with one or more vacuoles and a single nucleus, cause alcoholic fermentation (Plate 258). Yeast usually has little or no mycelium, and reproduces by budding. It results in production of yellowish surface froth or sediment that occurs in saccharine liquids (fruit juices) in which it promotes alcoholic fermentation, consists largely of cells of a fungus (as the saccharomyces, Saccharomyces cerevisiae), and is used in the making of alcoholic liquors and as a leaven in baking.

Principle

Plasmids requires hosts to replicate, hence it has to be inserted into bacteria. But efficiency of uptake of plasmid DNA by bacteria is low and will not do so without getting degraded. Therefore, to enhance the probability of uptake of the plasmid DNA by bacteria it is necessary to make the bacterial cells “competent” to absorb the plasmid DNA. One of the methods to increase efficiency of cell competence is given below. It should be noted that competent cells are more fragile than normal bacteria. Vortexing the cells, heating the cells above 42ºC or to 42°C for prolonged periods, or exposure of the cells to abusive treatments may kill them

Aim

To synthesize iron (III) oxide (Fe₂O₃) nanoparticles

Introduction

Iron oxide nanoparticles have a number of applications in catalysis, sensors and high sensitive biomolecular magnetic resonance imaging (MRI) for medical diagnosis and therapeutics. They can be synthesize by the bottom-up approaches including the sol-gel technique.

The global herbal industry faces significant challenges due to varying regulatory frameworks, quality control standards, and traditional practices. This talk aims to bridge the gap by promoting “Herbal Harmony”- a unified approach to standardizing herbal products worldwide. Experts from diverse f ields will converge to discuss: Harmonization of regulatory frameworks, standardization of quality control and assurance, validation of traditional knowledge, innovative technologies for herbal analysis and global market trends and opportunities by fostering collaboration and knowledge sharing, “Herbal Harmony” seeks to: enhance consumer safety and confidence, promote sustainable herbal trade practices, support evidence-based herbal medicine and to facilitate international collaboration and trade.

These instruments are used for surgery and need special care and attention for sterilization and storage.
 
    1.    Strabismus scissors : It is used for treating strabismus i.e. sectioning of affected muscle.
 
    2.    Me Guire corneal scissors : It is used for incising cornea
 
    3.    Graefe’s eye speculum : It is used for retraction of eyelids for examination of eye or for ophthalmic surgery.
 
    4.    Ziegler knife needle : It is used for separation of ocular tissues during ophthalmic surgery.
 
    5.    Iris hook : It is used for handling of Iris drug ophthalmic surgery.
 
    6.    Tenotomy scissors : It is used either for cutting the ocular ligament of eye or for removal of ocular sutures.
 
    7.    Dressing forceps : It is used for manipulation of eyelids, 3rd eye lid and other tissues of the eye.

Aim

To synthesize silver (Ag) nanoparticle.

Introduction

The Ag nanoparticles easily interact with other particles and increases their antibacterial efficiency. The Ag nanoparticles can be synthesized using various methods: biological, chemical electrochemical, γ-radiation, photochemical, laser ablation etc (Udapudi et al., 2012). The most popular preparation of Ag colloids is chemical reduction of Ag salts by sodium borohydride or sodium citrate (Udapudi et al., 2012).

Most of the bones of the skull are joined (articulated) with the adjacent bones by sutures. These are immovable joints (synarthroses). The uniting medium is a fibrous tissue and is called the sutural ligament. Most of these joints exist in the early life of the animals and as the age of the animal advances the adjacent bones fuse with each other.

1. Botanical Name : Securina leucopyrus syn. Flueggea leucopyrus

2. Family : Euphorbiaceae.

3. Common Name : Bushweed, Indian snow berry, White honey shrub

4. Name in Indian languages

Katupila, Shinar (Hindi), Pandharphali (Marathi), Mulluppulatti (Tamil), Cerimklaav (Malayalam), Pulugudu (Telugu), Bilchuli (Kannada), Parpo (Konkani), Humri (Gujarati), Shwetakambuja(Sanskrit), More pan (Kodwa).

5. Description

Bush weed is an erect shrub of family Euphorbiaceae. The plant grows up to 4 m tall with branches cylindrical or obtusely angular. Leaf is elliptic, obovate, or round papery to thinly leathery. Flower cymes arise in leaf axils or at leafless nodes. Flowers are tiny and yellowish. Fruit is a nearly spherical berry, about 4 mm in diameter, whitish when ripe.

Transformation

The uptake of DNA by competent cells is called transformation. Transformation involves:

Mix a small volume of DNA (usually 1-3 µl) with 100 µl of competent cells.

The mixture is incubated on ice for 30 minutes to allow the cells to take up the DNA.

The cells are then heat-shocked at 37 to 42°C for 1 minute, and cold shocked on ice for 2 minutes.

The heat shock/cold shock reverses the effect of the competent cell process, by healing the damage inflicted by the competent cell solution.

Add 0.5 ml of LB culture mediumz

Incubate the cells for 10 - 30 minutes at 37°C to aid the healing process.

This cell/medium mixture is then spread onto bacterial culture plates containing agar, LB, and a selection mechanism.

Failure in timely diagnosis of diseases and other pests has often been responsible for devastating losses. Reducing crop losses by keeping pests at bay is crucial to food security. Plant clinic is an innovative paradigm which plays a vital role in assuring food security and ushering prosperity by providing timely diagnosis and rendering necessary advice to the growers, gardeners and other stakeholders for managing pest problem in India. Plant clinics are all about plant health. Though the major role of plant clinic lies in diagnostics and advisory, the activities of plant clinic extend beyond plant clinic, with emphasis on extension, working more closely with farmers and organizations involved in promoting food production. The role of plant clinics beyond diagnostics and advisory. These are:

Radio script should be prepared in such a way that listener should feel that somebody is talking with him in a friendly manner and with informal style. For this purpose:
1. Collect the material from your experience.
2. Keep the purpose of the script and audience in mind.
3. Decide the method of presentation of information.
4. Considering the time available for the talk, the script should be prepared.
5. Generally we talk two words per seconds and 8 to 10 lines are required per minute having 5-6 words per sentences.
6. Time for talk is always limited hence select one idea at a time never include maximum technical information in the script.
7. Arrange the information in the sequences.
8. Include supporting and illustrated facts.
 

1. Introduction
All glassware used for bacteriological work must be meticulously cleaned and sterilized. Used and soiled glassware should be thoroughly washed to remove dirt and solid residues before drying and undergoing sterilization.
2. Cleaning of New Glassware
Borosilicate glass (e.g., Pyrex) and factory-washed soda glass require only standard washing before use. However, new, unwashed soda glass needs additional treatment to neutralize alkali content.
• Place the items (e.g., test tubes, Petri dishes, and flasks) in a suitable container, cover with a 1% tri-sodium phosphate solution, and bring to a boil.
• Remove the items, rinse with tap water, and immerse them in a 1% hydrochloric acid solution to neutralize alkalinity.
• Rinse thoroughly with tap water, followed by distilled water. Allow the glassware to dry before sterilization.
3. Cleaning of Used Glassware and Other Apparatus
• Autoclave all glassware contaminated by microorganisms (121°C for 15–20 minutes). This liquefies solid media, facilitating removal.
• Rinse the empty glassware with tap water, then warm water (50–55°C).
• Soak in a detergent solution (e.g., 1% washing soda or tri-sodium phosphate solution) and scrub with a brush.
• Rinse thoroughly with tap water to remove detergent traces, followed by distilled water.

    1.    Molar tooth cutter :  It is used to cut the extra sharp molar tooth in large animals (Fig. 7.1).