eChapters

I. FILL UPS

1. Somatic embryo encased with artificial endosperm and protective cover is called _____.
2. The term artificial seed was coined by_____.
3. The artificial seed technology was patented by_____.
4. The most common gelling agent used for artificial seeds is_____.
5. In desiccated system, the artificial seeds are coated with _____.

1. Botanical Name : Xeromphis spinosa (Thumb.) Keay

2. Family : Rubiaceae

3. Common Name : Thorny Bone-apple, Common emetic nut

4. Name in Indian languages

Mainphal (Hindi), Ghela, Khajkanda(Marathi), Madkarai (Tamil), Karacchulli (Malayalam), Marrga (Telugu), Kaarekaayi-gida (Kannada), Patova (Oriya), Madanah (Sanskrit), Kare pan (Kodwa).

5. Description

Xeromphis spinosa is a plant of family Rubiaceae. It is a spiny, deciduous shrub or small tree growing up to 7 metres tall. The plant sometimes adopts a climbing habit. The fruits matures in the month of June-July. The whole fruit expect the seed is consumed at ripe stage.

6. Origin and distribution

It is native of East Asia, Southern China, Indian subcontinent, Malaysia, Vietnam, Indonesia. It is found all over India except higher hills. It is also found in the Himalayas, up to an altitude of 1600 m. It is common in hilly area of Rajasthan.

A. Circular letter
• Circular letter is a quick and cheap method of communication.
• It is a letter reproduced and sent to many people by the extension worker, to publicize an extension activity (like meeting, exhibits etc.) or to give information on farm and home problems.
• Circular letter could be used as a mail before organizing any campaign or extension work.
• It is sent from extension wing weekly or fortnightly to maintain regular contact with the farmers depending upon

Bael is commonly known as Bengal quince, Indian quince, golden apple, holy fruit, stone apple and bel. It is more prized for its medicinal virtues than its edible quality.  The deciduous tree with trifoliate aromatic leaves is traditionally used as sacred offering to ‘Lord Shiva’. Fruit is a hard-shelled berry and very well-known for its medicinal properties due to marmelosin content. The ripe fruit is tonic, restorative, laxative and good for heart and brain. The pulp is often processed as nectar or squash. A popular drink called “Sherbet” in India is made by beating the seeded pulp together with milk and sugar. A beverage is also made by combining bael fruit pulp with that of tamarind. These drinks are consumed perhaps less as food or refreshment than for their medicinal effects. For medicinal use, the young fruits, while still tender, are commonly sliced horizontally and sun-dried and sold in local markets. The fruit, roots and leaves have antibiotic activity. The root, leaves and bark are used in treating snakebites.

Introduction

It is a well known fact that the soluble oxalates are broken down by rumen microbes from buffalo and cattle and one of the end product formed is bicarbonates.

The rumen microbes are obtained from a fistulated buffalo calf fed on wheat bhusa and sarson cake supplemented with 60 g potassium oxalate daily for about three months. Representative sample of rumen ingesta is drawn before feeding and mixed culture is prepared by the method as described Donefer et al., (1960). Mixed culture and basal nutrient mixture (Quicke et al., 1959) of pH 6.9 mixed in equal quantities. Fifty millilitres of this mixture are added into each incubation flask containing one gram of wheat straw, ground to 40 mesh as substrate. In the incubation flasks are added 35,100 and 550 mg oxalic acid in the form of potassium oxalate and 0.24 µCi sodium oxalate 14C. Blank (containing no substrate, no oxalic acid added) and two controls, one having substrate but no added oxalic acid and the other containing the above plus radioactivity, are also run alongwith. All the incubation flasks are taken in duplicate. The flasks are incubated at 39ºC for 24 h  in an incubator and at the end of this period microbial activity is stopped by the addition of few drops of saturated solution of mercuric chloride.

6.1 Introduction

Garden design encompasses the aesthetic beauty, artistry, knowledge of plant growth and development with basic maintenance of plants and other elements as required. Principles are the basic rules of designing a landscape. The basic principles of garden design are very simple; however, each is often referred to by more than one name. Principles of landscape garden keeps the garden lively and beautiful. The principles of gardening should be kept in mind always while designing a garden.

6.2 Axis

It is an imaginary line in landscape designs around which the garden is created striking a balance. In a formal garden the central line is the axis.

Awareness campaign should help educate your community on a specific subject by sharing basic information and providing examples. This will help community members have healthy conversations and increase awareness. This will begin the prevention process. Any awareness campaign should include following step;
1. Choose Your Topic Or Goals
2. Find Your Resources: Awareness campaigns take a lot of resources. You might have a set budget to implement your campaign. Money is not the only resource you will be using. Resources such as time, marketing and promotional material etc also needed for successful completion of campaign.
3. Segment Your Audience
4. Create a Schedule of Events, Programs, and Initiatives

There are countless biomarkers present in cells related to and determining the progress of a disease. Identifying these markers could aid in detecting cells with metastatic potential (Ramaswamy et al., 2003).

The elevation of cyclo-oxygenase (Cox-2) expression was observed in CMT cases through enzyme immunoassay (Queiroga et al., 2005). In further study, enhanced expression of BRAC1and BRAC2 genes, certain enzymes such as matrix metalloproteinases (MMP-2 and MMP-9) by immunohistochemical examinations, and Bcl-2 & Bcl-XL elevated level in dogs with mammary neoplasm have been reported by Queiroga et al. (2011b).

Indian agriculture now stands at a cross road. By 2050, Indian population will overtake that of China’s. The world population in the same year will be close to 9.5 billion. And to feed the human population of this planet, the agricultural production needs to be increased 60% over the present one by 2050. For India the target is 450 million tons by 2050.

This is a humongous task. This mammoth escalation of targeted food production has to be carried out with the constraints and barrier created by climate change, erosion of soil, bio-diversity, ecological disruption due to mindless anthropogenic action and wrong planning.

The production, productivity, income, livelihood, and ecological resilience in agriculture are not performing in perfect orchestration. They are in apparent contradiction. To produce 1 kilogram of rice, the equivalent water foot print is about 4000 litres. It is obvious that enhancing food productivity, there could be more disruption and stress on soil, water and bio-diversity. The ecological resilience is a subject of musical chaos and symphony amidst ecological function-stress-conservation-extraction-. Without depletion and disruption, it is simply impossible to initiate and foster agriculture production as well as productivity. But the question is what could be level of endurance? Wherein restoration and retention, ecological resilience will move in resonance and complementary manner.

Introduction
This method consists of mixing the microorganisms in a small amount of India ink or nigrosine and spreading the mixture over a clean slide. These two pigments are not really bacterial stain and do not penetrate the microorganisms. These pigments make the background dark thus making organisms transparent against dark background. This method is useful for determining cell morphology and accurate size as no shrinkage of cells due to heat.
Materials
1. Bacterial culture (B. subtilis)
2. Microscopic slides (Clean, Grease Free)
3. Inoculating needle
4. Cedar wood oil
5. Microscope
6. Xylene

Principle

    Inorganic salts are relativity insoluble in aromatic solvents which has been detrimental to the estimation of 45Calcium by scintillation counting. This technique inolves the counting of 45Calcium as calcium 2-ethyl hexanoate in a solution of toluene phosphor; and the counting as calcium chloride in a tertiary mixture of absolute ethyl alcohol-hydrochloric acid-toluene-phosphor.

Reagents
    1.    ⁴⁵Calcium. A ⁴⁵Calcium solution supplied as calcium chloride in hydrochloric acid solution, is suitably diluted.
    2.    Calcium chloride, 0.045 N, is prepared by dissolving ACS grade calcium carbonate, dried to constant weight at 110ºC, in 5 N hydrochloric acid.
    3.    Calcium acetate, citrate, lactate, acid phosphate, chloride and hydroxide.
    4.    Ammonium oxalate solution, 4 percent
    5.    2-Ethyl-hexanoic acid.
    6.    Toluene-diphenyloxazole solution, 2g of diphenyloxazole are dissolved in 500 ml of toluene.

1. Botanical Name : Zizyphus rugosa Lamk

2. Family : Rhamnaceae.

3. Common Name : Wild Jujube, wrinkled jujube

4. Name in Indian languages

Churna, Suran (Hindi), Toran, Turan (Marathi), Totari (Tamil), Thodali, Tutari (Malayalam), Gottichettu (Telugu), Belahadu, Mulluhannu (Kannada), Kotte pan (Kodwa).

5. Description

Zizyphus rugosa is a large armed, spiny, evergreen tree growing around 5 - 9 metres tall with large elliptic usually subcordate leaves, paniculate flowers and wood is reddish, moderately. This plant is found chiefly in deciduous and semi-evergreen forest, hills and mountains below 1200 m altitude. The leaves are simple, and are broadly elliptic and short tips. Fruits are globose or pyriform, white when ripe. Whole fruit expect the seed is consumed at ripe stage. It is found in degraded forest areas of entire of Western Ghats. The plant is harvested from the wild for local use as a food and a medicine.

The following is the plan of action to hold Animal Health Camps
1. Attempt will be made to hold at Gram Panchayat level.
2. Before 2-3 days monadi of camps must be do.
3. The camp will be held for 1 day. It will start by 7 AM and close at 2 PM.
4. The venue should be selected so that it is in the central location to get cases from 3-4 villages in order to provide facilities of clinical examination, pathological tests, etc.
5. The venue should be centrally located and easily accessible to the general public.

A thorough history with complete physical examination including careful palpation of the mammary glands should be performed. As there are chances that dogs may be suffering from any other systemic ailments during middle to old age. In dogs with multiple tumors, all tumors should be excised and their location and size should be noted. Histopathological examination of the excised tumors should be carried out to classify mammary gland tumors. Mammary tumors are classified based on some of the parameters i.e., tumor size, lymph nodes, metastasis. Therefore, to stage a dog with mammary tumors information of these parameters needs to be collected and recorded. If lymph nodes are palpable and/or clinically enlarged, fine-needle aspiration should be performed for cytological evaluation. The technique is less invasive but with higher accuracy. Thoracic radiographs remain the mainstay for diagnosing the metastatic condition. The mammary tumors most commonly metastasize into lungs in dogs. The staging of CMTs becomes necessary as it is likely to confer some prognostic significance and provide guidance to veterinarians to make treatment decisions.

Ber is most commonly found in the tropical and sub-tropical regions of the world. Originally native to India, it is now widely naturalized in tropical region from Africa to Afghanistan, China, Malaysia, Australia and in some Pacific regions. The major production regions for Indian jujube are the arid and semi arid regions of India. It is also known as ‘King of Arid Fruits’ and ‘Poor Man’s Fruit’. In Philippines it is called manzana or manzanita (“apple” or “little apple”). Fully ripe fruits are less crisp and somewhat mealy; overripe fruits are wrinkled, the flesh buff-coloured, soft, spongy and musky. 

(Method of Olbrich et al., 1971)

Jones and Ekman (1960) showed that ¹⁴⁴Ce is absorbed to a very small extent in the gut of the ruminant and suggested its use as a marker in digestibility trials. Huston and Ellis (1968) studied the affinity of radioactive cerium for feedstuffs and digesta particles in vitro experiments and found that cerium is rapidly absorbed onto and remained tenaciously bound to  digesta particles. Ellis and Huston (1968) tested radioactive cerium as a particulate flow marker in sheep with favourable results.

Experimental Procedure
Three Holstein bulls weighing 315, 239 and 305 kg and one Holstein steer weighing 327 kg are used. Twice daily, at 9.00 am and 9.00 pm, they are fed 3.4 kg of a balanced ration, meeting NRC requirements for normal growth and maintenance.

Surgical removal is the routine treatment method for mammary tumors, however, there is no consensus on the optimal procedure. There are supporting factors for every surgical procedure conducted for mammary tumors including lumpectomy (removal of tumor only), mastectomy (affected gland removal only), radical mastectomy (complete mammary gland and associated lymphatic tissue removal) and modified radical mastectomy (removal of complete mammary gland, lymph nodes and associated chest muscles).The more extensive procedures do not increase survival in dogs when compared to the other procedures, and the benefits of the simpler procedures are clear. Further, the radical mastectomy increases the disease-free interval but not the survival duration in cats.

This chapter introduces soil testing as an essential component of scientific nutrient and land management. It traces the establishment and expansion of soil-testing laboratories in India and explains their role in assessing soil fertility, productivity and physical, chemical and biological conditions. Soil testing helps determine suitable fertilizer and amendment requirements while preventing the excessive or unbalanced application of nutrients. The chapter emphasizes that fertilizer recommendations must reflect differences among soils and fields. It also introduces representative soil sampling as the foundation of dependable laboratory results and outlines important precautions concerning equipment calibration, clean glassware, contamination prevention and safe laboratory practices.

The breadfruit is believed to be native to New Guinea. It is grown throughout the tropics. Breadfruit is a staple food in many tropical regions. Raw breadfruit has a very light fragrant taste that is somewhat like a guava, but not as sweet. The texture of raw breadfruit is rather tough and rubbery. It is used more as a vegetable than as a fruit. It can be eaten ripe as a fruit or under-ripe as a vegetable. Soft or overripe breadfruit is best for making chips and biscuits. The dried fruit can be made into flour. Its flour is much richer than wheat flour in lysine and other essential amino acids. Breadfruit is high in calories and contains a good amount of vitamin C and potassium with smaller amounts of calcium, iron and magnesium. The wood is yellowish or yellow grey with dark markings or orange speckles; light in weight; not very hard but strong, elastic and termite resistant and is used for construction and furniture. Fiber from the bark is highly durable and is used for clothing in Malaysia.

Plant nutrients are naturally components of the soil orchestrated by weathering processes and that the abundance of these metals in our soil environment now-a-days is due to numerous anthropogenic activities. In the environment, heavy metals are partitioned among the various environmental compartments such as water, soil, suspended solids and biota. Processes such as dilution, dispersion, sedimentation, adsorption and desorption govern their distribution. They also exist in colloidal ionic particulate and dissolved phases,  have a high affinity for humic acids, organic colloids and oxides coated with organic matter. The soluble forms are generally ions or unionised organo metallic chelates or complexes. The solubility of metals in soil and groundwater is predominantly controlled by pH, amount of metal, cation exchange capacity, organic carbon content, the oxidation state of the mineral components and the redox potential of the system.