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Denaturing polyacrylamide/urea gels in TBE buffer

Prepare 20ml of a 5% polyacrylamide gel containing 7M urea by adding:

47.5% acrylamide:

2.5% bis-acrylamide solution 2ml

10M urea 14ml

10X TBE buffer 2ml

10% freshly prepared ammonium persulfate 0.2ml

deionized water 1.8ml

Aim

To synthesize Zero valent (Fe) (nZVIs) iron using tea leaf extract.

Introduction

In this approach extracts of natural products (in most cases green tea leaves) with high antioxidant capacities are used. The compounds present in these extracts react with Fe (III) in solution to form nZVIs (Nadagouda et al., 2010).

Group Discussion
Group discussion is the method by which two or more persons meet, express or suggest their ideas for getting the solution to the felt problems by them.
Objectives of group Discussion
• To develop the democratic leadership among the members.
• To conduct the systematic group discussion to solve the problems.
• To give opportunity to each member to share experiences.
• To promote the habit of talking, thinking, group planning and group action.
• To identify the group and focus the public interest and mobilize them for group action.

Carambola or starfruit is native to Indonesia. The fruit has ridges running down its sides (usually five) which in cross-section resembles a star, hence its also known as starfruit. It has a waxy, golden yellow to green coloured skin with a complicated flavour combination that includes plums, pineapples and lemons. Carambola consists of two main types of varieties viz.  sour and sweet. The sour types contain as much as 1 per cent acid and the sweet types have low acid (0.4 per cent) content with 5 per cent sugars.

The requirement of micronutrients is always in low amounts while their moderate decrease causes the deficiency symptoms and a moderate increase causes toxicity. In other words, there is a narrow range of concentration at which the elements are optimum. Any mineral ion concentration in tissues that reduces the dry weight of tissues by about 10 per cent is considered toxic. Such critical concentrations vary widely among different micronutrients. The toxicity symptoms are difficult to identify. Toxicity levels for any element also vary for different plants. Many a times, excess of an element may inhibit the uptake of another element.

General Description of An Animal
    1.    Age      
    2.    Sex                          
    3.    Body weight

The principle of silver nitrate staining of proteins is based on the reduction of silver ions to its metallic form. (Silver ions in alkaline conditions bind with proteins through å-amino group of lysine and sulphur groups of cysteine and methionine residues). When the complexed silver ions are reduced in the presence of formaldehyde, they become metallic silver, which is seen on the gel as bands. Staining of proteins with silver nitrate after SDS-PAGE is quicker and ~ 10 times more sensitive than Coomassie brilliant blue staining and that is the reason it is widely used in research laboratories these days.

The procedure is as follows:

One of the most promising areas of veterinary research these days is cancer immunotherapy and the development of CMT vaccines. DNA vaccines, also known as gene vaccines, are designed to transfer functional genes to target cells, allowing them to express functional proteins. Because endonucleases may easily access bare DNA, it requires a reliable and safe delivery route, which might be biological or non-biological. Different delivery strategies have been tried in human breast tumors to transport tumor antigens such Mucin-1 (MUC-1), Twist, HER-2, mammaglobin-1 and p53, as well as immunostimulatory molecules like IL-12 and IL-6. There have been various studies/ trials in canine animals using different molecules like IL-12, HER-2 and IFN-γ as vaccine candidates, with no clinically relevant outcomes or longlasting cell mediated and humoral immune response outcomes

Ornithosis

Ornithosis is caused by Chlamydia and characterized by enlargement of liver and spleen, serofibrinous pericarditis, air sacculitis and enteritis. This is also known as chlamydiosis, psittacosis and is more common in parrots, pigeons and other psittacine birds.

Etiology

• Chlamydia psittaci group B

• Size 0.3 to 1.5 m

• Disease spreads from wild birds

a) A Key GDP Contributor: Dairy and Livestock sector form the backbone of agriculture with 28 per cent contribution in GDP and the progress in this sector is dependent on innovative measures to increase the value for farmers as well as customers.

b) Doubling Farmer’s Income : Doubling Farmer’s Income by 2022 can be achieved by improving crop and livestock productivity, efficiency to utilize resources, increased intensity of cultivation and diversification in high value cash crops, processing and value addition of food and animal products. This price realization can be assured through market reforms and efficient market linkages.

c) Unorganised sector – a challenge : The Indian dairy sector is still characterized by strong presence of unorganized sector. Apart from the Cooperative dairy sector, in the last few years there has been a considerable growth in Private Dairy sector and Milk Producers Organization like Maahi in Gujarat. If small dairy farmers are provided with sufficient ‘value addition’ training and support, they can increase their income considerably.

d) Value addition : Further, the milk processing plants can also go for value addition and innovation and in turn pay remunerative prices to milk producers.

The English name of cashewnut derives from the Portuguese name for the fruit of the cashew tree, Caju. It is native to the coastal areas of north-eastern Brazil. It was introduced in Goa by the Portuguese in the 16th century mainly to control soil erosion and to use in afforestation programmes but now it has become an important cash crop of Goa. The fruit of the cashew tree is an oval or pear-shaped accessory fruit known as ‘cashew apple’ sometimes called a pseudocarp or false fruit which develops from the receptacle of the cashew flower.  The cashew nut is a popular snack with a rich flavour that is often eaten on its own, lightly salted or sugared. Cashew nuts are also sold covered in chocolate. In Goa, the cashew apple is mashed and juice is extracted and kept for fermentation to prepare beverage called fenny. 

Toxic elements like Cadmium (Cd), Fluoride (F), heavy metals, radioactive metals are associated with mainly rock phosphates, other organic wastes like sewage sludge and pollutants. Their chemistry is similar to plant nutrients and replace them in their physiology leading to reduced uptake of nutrients, productivity and contaminated food chain.

In the realm of cell culture, the quality and composition of the growth media and reagents play a pivotal role in growth and sustaining cells in vitro. The preparation of these essential components requires precision, attention to detail, and adherence to the strict protocols to ensure optimal cellular growth, viability, and experimental reproducibility. This introduction serves as a foundational overview of the critical aspects involved in the preparation of media and reagents for cell culture experiments. Furthermore, the preparation of media and reagents for cell culture necessitates adherence to stringent quality control measures to ensure consistency and reproducibility across experiments. Validation of media batches, sterility testing, and periodic quality assessments are essential practic

 Objectives
• Understand the meaning of the communication process.
• Learn the various types of communication.
• Explore different models of communication.
• Analyze how communication flows in different contexts.
Meaning of Communication Process
The communication process refers to the systematic steps through which a message is conveyed from a sender to a receiver. It involves the transmission of information through a medium, with feedback confirming whether the message has been received and understood correctly.
Elements of the Communication Process
The communication process consists of the following elements:
• Sender: The person or entity initiating the message.
• Message: The content or information to be communicated.
• Encoding: The process of converting the message into symbols, language, or signals that can be understood by the receiver.
• Medium/Channel: The pathway through which the message is transmitted (e.g., face-to-face, email, phone).
• Receiver: The person or entity for whom the message is intended.
• Decoding: The process by which the receiver interprets and understands the message.
• Feedback: The response of the receiver that indicates whether the message was understood

In GADVASU, under the DBT sponsored project on “Development and evaluation of serodiagnostic assay for timely diagnosis and prognosis of mammary tumors”, we have generated and used hyper-immune sera raised against certain canine recombinant mammary tumor associated proteins for the standardization of sandwich and indirect ELISA for serodiagnosis of canine mammary tumor. The sensitivity and specificities of the assay’s ranges between 85-88% and 80-85%, respectively. However, an antibody against a single marker when used for different histotypes of CMT, its specificity reduced significantly. Multiplex assays are capable to detect many specific antigens in a single run whereas a traditional ELISA is used only to detect a single antigen. It has high-throughput potential, providing more results per sample, with lower sample volumes and with utmost sensitivity and specificity. Attempts are being made to increase the sensitivity and specificities of the assays by multiplexing various markers together (Fig.4).

Spirochetosis

Spirochetosis is caused by a spiral shaped organism Borrelia anserina and characterized by greenish diarrhoea, enlarged and mottled spleen, small necrotic patches on liver and linear haemorrhage in proventriculus. This disease is transmitted by a tick Argas persicus and thus also known as “tick fever” or “tick paralysis”.

Etiology

• Spirochete Borrelia anserina

• Spiral shape 8-24m long and 0.2 to 0.3 m wide microorganism having 8-11 spirals.

• Stained with aniline dyes

Principles
The quality of foods can be defined as the ‘composite’ of those ‘characteristics’ that differentiate individual unit of a product and have significance in determining the degree of acceptability of that unit by the consumer. The overall quality refers to technological, physiological, chemical, microbiological, nutritional; and sensory parameters to achieve the wholesome food. Sensory quality attributes such as taste, colour, texture, flavour and overall quality of any food products were evaluated by 9- point Hedonic rating test is used for the purpose of evaluation. This test measures the consumer’s acceptability.

Cell Lysis Procedure

Pellet the cells in a microfuge tube: add 1.5 ml culture, centrifuge for 15 seconds at maximum speed, discard the supernatant, and then repeat the procedure. This will result in the cells from 3 ml of culture in a single tube.

Resuspend the cells in 1 ml Lysis buffer.

Take 300 µl aliquot for Bradford protein assay.

Add 50 µl lysozyme, and mix gently.

After 10-20 minutes at room temperature, microfuge for 10 minutes to pellet cell debris.

Use supernatant for LDH assay.

Mammary tumors are the most common type of neoplasia found in female dogs with high percentage of malignancy and rate of mortality. The rate of incidence of these mammary neoplasia and dysplasia is continuously advancing nationally and internationally over the last 45 years. There are different risk factors such as age, diet, hormonal exposure and breeds, which are required to be monitored in order to control the incidence of CMTs. In addition to conventional therapies such as surgical excision, radiotherapy and chemotherapy, additional precision therapeutic agents targeting TKIs, p53 tumor suppressor gene, cyclooxygenases, epithelial-mesenchymal transitions and various hormones/ hormonal receptors could be beneficial for the treatment of CMTs. Scientific efforts are also being made to develop different biomarker panels and tests for prognosis/diagnosis of the diseases as well as to develop vaccines against CMTs.