eChapters

INTRODUCTION

Potato is a vegetatively propagated crop mainly through tubers. Seed is the costliest input, which constitutes nearly half of the production cost. Due to the high cost of seed, seed replacement rate is low, which results in build up of various tuberborne diseases.To maintain high tuber yield, multiplication is done through “Seed Plot Technique” (SPT), which involves seed production in low aphid pressure period to minimize transmission of viruses and also adopt a set of cultural practices aimed at minimizing disease spread. The production of healthy seed tubers through SPT is expensive and the low rate of tuber multiplication (normally 68 times) provides only a limited quantity of quality tubers. Further, the low aphid areas suitable for producing healthy seed in the country lie in the northern plains and high hills (> 1800 m above mean sea level). Changing climate scenario is likely to shrink the window for potato cultivation and increase pathogen/vector attacks across the country thereby seriously limiting quantity and quality of seed in the country. An alternative technology of true potato seed (TPS) or use of botanical seed has shown great potential for producing both diseasefree healthy (quality) and cheaper planting materials for commercial potato production. This chapter describes the classical TPS technology and two novel potential TPSbased technologies in potato propagation.

True protein nitrogen is also termed a Albuminoid nitrogen in animal feeds and fodders and human foods. If material (Such as seeds, seed residue or oil cake) is rich in alkaline phosphates, 1-2 ml of 10 percent solution of potash alum is added to decompose them. If this is not done Cu3 (PO4) and free alkali may be formed and the protein-copper precipitate may partially dissolve in the alkaline liquid.

Citrus reticulata

• A highly polyembryonic species of Chinese origin
• Mandarin occupies 43% area and 42% under citrus spp.
• Seeds of citrus plants do not have dormancy so they should be sown immediately after extraction.
• Blooms three times a year.

  a) February flowering – Ambe bahar

  b) June flowering – Mrig bahar

  c) October flowering – Haste bahar

• Mandarins are highly susceptible to waterlogging.
• Rootstock for HDP – Troyer citrange (1.8 × 1.8 m2)
• Best time for pruning – Last winter or early spring.

Cell counting is the fundamental techniques in various fields of biology, medicine and biotechnology.

Importance of cell counting

1. Research: Cell counting is a critical component of biological and medical research. It helps scientists understand the behavior of cells in various contexts, such as studying cell proliferation, differentiation, and response to stimuli.

2. Disease diagnosis: Monitoring various medical conditions, including infections, autoimmune diseases, cancer and blood disorders. Complete blood counts (CBCs) are routinely used to assess the levels of different blood cell types and assess disease conditions.

3. Stem Cell Research: Cell counting plays a significant role in stem cell research, where precise quantification of stem cells is necessary for various applications, including regenerative medicine and tissue engineering.

4. Cell Culture: Cell counting is integral part to determine seeding density. Seeding of cells to available surface area for growth is important consideration for successful culture.

5. Cell-Based Assay: The cell-based assays are used for drug screening, toxicity testing, and evaluating the efficacy of new compounds. Accurate cell counts are essential to ensure the reliability of assay results.

Kingdom: Protozoa
Phylum: Euglenozoa
Class: Kinetoplasta
Order: Trypanosomatida
Family: Trypanosomatidae
Genus: Trypanosoma
Members of the genus Trypanosoma are responsible for trypanosomosis in animals and human beings and are found in the blood vascular system and tissues all over the world. All the species of trypanosomes are transmitted biologically or mechanically through an arthropod vector except T. equiperdum, which is transmitted through coitus in equines.

Abstract

The recent tsunami occurred on 26^thDecember 2004, caused great devastation along the east coast of Tamil Nadu, India. As the said tsunami has caused colossal damages, the impact assessment has become essential for tsunami mitigation measures. In this context, the extent of tsunami inundation, loss of lives, property loss and damages to physical and natural resources have been assessed in the tsunami affected area between Ennore and Mahabalipuram along the east coast of Tamil Nadu.

IRS LISS III PAN merged satellite data and IKONOS data were used for mapping the pre-tsunami geomorphology and land cover conditions and IRS-P6 PAN merged satellite data were used to map the post – tsunami terrain conditions including the inundations. From the study it is inferred that wherever buildings and population are denser the loss is more. Hence, vertical expansion may be needed as an alternative or to supplement horizontal expansion. Bio-shield along coastal areas, involving raising of mangrove forests, planting of casurina, salicornia, palms, bamboo and other tree species and halophytes which can grow near the sea will serve as speed-breakers for the up rising waves.

Keywords: Tsunami, Damage Assessment, Mitigation Measures, Chennai region.

Introduction

On 26^th December 2004, the Indian coastline experienced the most devastating tsunami in recorded history. The tsunami was triggered by an earthquake of magnitude Mw 9.3 at 3.316ºN, 95.854°E off the coast of Sumatra in the Indonesian Archipelago at 06:29 hrs making it the most powerful in the world in the last 40 years. In recent years increasing the vulnerability to natural disasters of developing countries is a major reason for concern. The potential loss of human lives and property due to natural hazards is very high in the urban areas of these countries. The Information obtained from conventional tsunami hazard maps does not always provide sufficient data for users .needs. The difficulty in presenting more significant information on tsunami hazard maps in conventional paper format is due to the limited available physical space of that format. Printing more information on such paper maps tends to confuse rather than provide more needed information to users. Furthermore, these kinds of maps provide little opportunity for users to ask for additional information, to determine the relationships between different kinds of information and important quantitative analyses. Furthermore, Tsunami hazards have important temporal attributes. Presenting these attributes on the map; e.g. the sequence of volcanic eruptions in a region or the frequency of tsumani in a particular area during a particular period of time is very difficult.

Abstract

Ennore is an industrial region having many industries on chemicals, fertilizers, refineries etc., discharging their effluents into the Buckingham canal. Present study focuses on the impact of tsunami on water quality of Buckingham Canal segment found to the north of Chennai city, Tamil Nadu, India, in addition to the human interferences. Water samples were collected from surface and near bottom at five locations covering about 20km stretch of the Buckingham Canal during high tide on the spring tide day before (December 2004) and after (January 2005) the mega tsunami. Various water quality parameters measured were pH, Temperature, Salinity, Dissolved Oxygen, Suspended Particulate Matter, Nutrients and Chlorophyll. The maximum depth observed in the canal was 2.5m in December, whereas a shallow depth of less than 0.5m was recorded in January. The water quality analyses confirmed the incursion of saline water (upto 32ppt) into the Canal. Not much variations were observed in Dissolved Oxygen and pH. Because of the organic pollution in the canal, the water was rich in nutrients before tsunami, whereas due to the large quantity of seawater invasion, dilution had occurred after tsunami. High turbidity was observed in January 2005 indicating the tsunami induced disturbance and resuspension of the sediments from sea bed.

Keywords: Tsunami, Ennore, Tamil Nadu, Buckingham Canal.

15.1 Introduction

A tsunami is a series of waves created when a body of water, such as an ocean, is rapidly displaced. Earthquakes, mass movements above or below water, volcanic eruptions and other underwater explosions, landslides, large meteorite impacts and testing with nuclear weapons at sea all have the potential to generate a tsunami. The effects of a tsunami can range from unnoticeable to devastating. The term tsunami comes from the Japanese words meaning harbor (“tsu”,) and wave (“nami”,). The term is created by fishermen who returned to port to find the area surrounding their harbor devastated, although they had not been aware of any wave in the open water. Tsunamis are common throughout Japanese history; approximately 195 events in Japan have been recorded.

A tsunami has a much smaller amplitude offshore, and a very long wavelength (often hundreds of kilometers long), which is why they generally pass unnoticed at sea, forming only a passing “hump” in the ocean (Fig. 15.1). Tsunami have been historically referred to as tidal waves because as they approach land, they take on the characteristics of a violent onrushing tide rather than the sort of cresting waves that are formed by wind action upon the ocean (Iwan, W.D., 2006).

Abstract

During natural disasters, mostly panic and curiosity lead to greater causalities. But the tsunami is such a disaster which no one have witnessed during their life time nor heard of from their ancestors. On 26, December 2004, tsunami has struck all of a sudden many South Asian coasts including the east coast of Tamil Nadu and Andhra Pradesh causing greater devastation and causalities. This historical event has taught us many lessons and the paper deals with some aspects related to tsunami from the citizen’s view point.

Keywords: Natural calamities, Tsunami mechanisms, Mitigation strategies, Citizen’s perspective.

INTRODUCTION

The tsunami that ravaged the coasts of South India had left tell tale signs that affirm the need for early warning systems as well as well-planned mitigation measures for the benefit of the affected. Over the last 12 months, since the tsunami struck the east coast of India on 26 Dec. 2004, numerous seminars, discussions, programmes and project proposals have been deliberated on various aspects that deserve attention. Government agencies, self-help groups and non-governmental organizations (NGOs) have done commendable post-tsunami work. All sections of the society have responded both emotionally and rationally to the event in an unprecedented manner. In retrospect, to the informed citizen – one who has heard about the event, read about it and discussed it with others, though not have had the ‘experience’ of the natural disaster by himself / herself - what do all these mean ? the tragedy and its aftermath. This paper documents the issues that a citizen would like to ponder over so as to understand the various dimensions of the post-tsunami activities and draw a few lessons that the tragedy had taught us. The major issues pertain to (i) the mechanisms (ii) the coastal vulnerability and the need for assessment (iii) mitigation strategies (iv) media exposition (v) communication networking and (vi) educational facilitation.

The phenomenon we call tsunami is a series of large waves of extremely long wavelength and period usually generated by a violent, impulsive undersea disturbance or activity near the coast or in the ocean. A tsunami is a very long-wavelength wave of water that is generated by sudden displacement of the seafloor or disruption of any body of standing water.  Tsunami are sometimes called “seismic sea waves”, although, as we will see, they can be generated by mechanisms other than earthquakes.  Tsunami have also been called “tidal waves”, but this term should not be used because they are not in any way related to the tides of the Earth.  Because tsunami occur suddenly, often without warning, they are extremely dangerous to coastal communities.

Thrips: Thrips tabaci : Tuberose thrips Franklinella schultzei: Thripidae

These minute insects, preferring hot humid weather, rasp and suck the cell sap, causing bronzing, withering and drying up of leaves and flowers. This is the most serious pest on tuberose. Damage to young plants results in stunted growth.

Introduction

Two-thirds of the population make their living from agriculture, which also supplies raw materials for other businesses. To feed the growing population and decrease hunger and poverty, agricultural output must be increased. Various farming systems coexist in India depending on the resources available, agricultural methods, and location-specific demands of people to meet their needs for food, fuel, and fibre. An intricate, interconnected matrix is the integrated farming system (IFS) of soil, plants, animals, tools, equipment, labour, capital, and other inputs that are partially controlled by farming families, with varied degrees of political, economic, and institutional power and resting elements that affect farms. In India, the size of farm holdings has dropped over time.

Tuber crops grown in India include Cassava, Sweet potato, Dioscorea, Colocasia, Amorphophallus and minor tuber crops like Chinese potato, Arrowroot, Canna, Yam bean etc. Biotechnology can contribute solution to the major problems and realize greater benefits for tuber crops. Traditional breeding in tuber crops is difficult due to their heterozygous nature, low fertility, out crossing coupled with strong inbreeding depression. Many of the agronomic traits are polygenic and/or recessive which make breeding for such characteristics complicated. Biotechnology is a powerful tool to complement traditional breeding. Developments in the field of tuber crops biotechnology are discussed below.

Root and Tuber crops are the most important food crops of man after cereals. Tuber crops are efficient producers of carbohydrates especially under suboptimal conditions of rainfall, fertility and low input conditions encountered in tropics. The group contributes about 6 % of the world’s dietary calories. In fact, one out of 6 kg of human food comes from roots and tubers. Tuber crops find an important place in the dietary habits of small and marginal farmers especially contributing to food security of tribal populations. Tuber crops grown in India include Cassava, Sweet potato, Dioscorea, Colocasia, Amorphophallus and minor tuber crops like Chinese potato, Arrowroot, Canna, Yam bean etc. The biotechnology can contribute to the solution of the major problems and realize greater benefits for tuber crops.

1. Tuber crops Starches

Tropical root and tuber crops are rich sources of starch (Ghosh et al., 1988; Chadha and Nair, 1994; Moorthy, 1994) besides many vitamins, minerals etc (Table 1). In addition to food applications, they have important industrial uses; especially, cassava in view of the considerable starch content. Cereals form the major source of commercial starches in most developed countries, but cassava (Manihot esculenta Crantz) and to a lesser extent, sweet potato (Ipomoea batatas Lam.) tubers are used for starch extraction in India, Brazil, Thailand, Indonesia, Philippines and China.

Tropical tuber crops are an important group of subsistence crops in tropical and subtropical zones. Carbohydrates synthesised and stored in the form of starch in underground tubers are used as food by millions in under-developed countries. It is also used as raw material by many industries. Cassava, sweet potato, Chinese potato, edible aroids like elephant foot yam, taro, tannia and yams like greater yam, lesser yam and white yam are major tuber crops grown in India.

Tuber crops are mainly grown as rainfed with minimum cost of production. All are propagated through vegetative means and require fertile and well drained loose soil for proper root development. All roots crops require high dose of potassium for high productivity.Cassava is the most important starchy root crop grown in the tropics and is mainly cultivated in southern peninsular India. Introduced during seventeenth century by Portuguese, the crop played a significant role to overcome food shortage among the low income group of people in Kerala. Underground tuber is rich in starch and mainly consumed after cooking. Processed products like chips, sago and vermicelli made of tapioca are also popular in the country. Being easily digestible, it forms an important ingredient in poultry and cattle- feeds. It is also widely used for production of industrial alcohol, starch and glucose.

Tropical tuber crops are an important group of subsistence crops in tropical and subtropical zones.  Carbohydrates synthesised and stored in the form of starch in underground tubers are used as food by millions in under-developed countries.  It is also used as raw material by many industries.  Cassava, sweet potato, Chinese potato, edible aroids like elephant foot yam, taro, tannia and yams like greater yam, lesser yam and white yam are major tuber crops  grown in India.

Tuber crops are mainly grown as rainfed with minimum cost of production.  All are propagated through vegetative means and require fertile and well drained loose soil for proper root development. All roots crops require high dose of potassium for high productivity.

6.1. Potato (Solanum tuberosum L.)

Potato, Solanum tuberosum, is a starchy, tuberous crop belonging to the Solanaceae family. The name potato originally referred to a type of sweet potato, batata, in Spanish. Potatoes are occasionally referred to as “Irish potatoes” or “white potatoes” in the United States, to distinguish them from sweet potatoes. The potato was believed to be first domesticated in the region of southern Peru and extreme northwestern Bolivia between 8000 to 5000 BC. The potato diffused widely after 1600, becoming a major food resource in Europe and East Asia. Greater geographic mobility of farmers led to the rapid spread of potato cultivation throughout China, and India. China is now the world’s largest potato producer and nearly one third of the world’s potatoes are produced in China and India.

Today potato is the fourth major food crop after rice, wheat and maize in the world. In India potato is a very important crop and are mainly produced in the states of Uttar

Cultivars/Varieties: Most of the improved potato varieties were developed by CPRI, Kufri, Shimla. Most of the earlier varieties released as clonal selections derived from foreign varieties have been replaced with high yielding new varieties from the same institute. The popular varieties under cultivation now are Kufri Sindhuri, Kufri Chandramukhi, Kufri Jyothi, Kufri Lauvkar, Kufri Muthu, Kufri Dewa, Kufri Badshah, Kufri Bahar, Kufri Lalima, Kufri Swarna, Kufri Jawahar, Kufri Ashoka, Kufri Megha, Kufri Sutlej, Kufri Pukhraj, Kufri Chipsona-1, Kufri Chipsona-2 and Kufri Giriraj.

Uses

Potatoes are used in different ways like boiled, fried, baked and roasted. Potatoes are used to brew alcoholic beverages such as vodka, potcheen, or akvavit.They are also used as food for domestic animals. Potato starch is used in the food industry as, for example, thickeners and binders of soups and sauces, in the textile industry, as adhesives, and for the manufacturing of papers and boards. Many companies are exploring the possibilities of using waste potatoes to obtain polylactic acid for use in bioplastic products; other research projects seek ways to use the starch as a base for biodegradable packaging. Potato skins, along with honey, are used in folk medicine for burns in India. Burn centers in India have experimented with the use of the thin outer skin layer to protect burns while healing. The potato is best known for its carbohydrate content, which is in the form of starch. Because of its high starch content, it is a staple food in several parts of world including in some parts of India. A small but significant portion of this starch is resistant to digestion by enzymes in the stomach and small intestine, and so reaches the large intestine essentially intact, thereby, increasing its physiological significance. Besides these the potato also provides various other nutritional constituents to the body. Potato is also processed into various products like chips, powder, pappad, savory items.