eChapters

Principle
Chutney and sauces are important food products prepared both in homes as well as commercially in India. They improve the digestion and are good appetizers. Tomatoes are a good source of vitamin C. Fresh tomatoes are very refreshing and appetizing but can not be stored for a long period. Often they are sold at distress prices during the peak harvest season and nearly 25% of the produce is spoiled due to mishandling and improper processing facilities. Such losses can be reduced by converting fresh tomatoes in to delicious product like paste, puree, juice, ketchup/sauce and powder. Generally, the sugar content in ketchup/sauce varies from 10-26%. The salt content of the product should be 1.3 to 3.4%. Good quality vinegar is essential for the preparation of high quality sauce/ketch up. It should contain 5.0-5.5% acetic acid or glacial acetic acid (100% acetic acid) and should be added when the product has thickened sufficiently. Tomato sauce/ketchup generally contains 1.25-1.50% acetic acid. In order to increase the viscosity and prevent the separation of pulp from clear juice, pectin can be added to extent of 0.1-0.2% pectin by weight of the finished product. Add 0.025% sodium benzoate as preservative to the product before bottling and then pasteurize.

After preparing the desired DNA molecule using E.coli, the plasmid can be transferred into Agrobacterium by this method. Efficiency is low (~10³ transformants per mg of DNA) but the method is reliable and very rapid. This method also eliminates much of the plasmid rearrangement that occurs during triparental mating.

Procedure

Grow an Agribacterium strain containing an appropriate helper Ti plasmid in 5 ml of LB medium 16 hours at 28ºC.

Add 2 ml of the culture to 50 ml LB medium in a 250 ml flask and shake (~250 RPM) at 28ºC until an OD₆₀₀ of 0.5 to 1.0 is obtained.

Chill the culture on ice. Centrifuge the cell suspension at 1000 g for 30 minutes at 4ºC.

Discard the supernatant. Resuspend the cells in 1 ml of 20 mM CaCl₂ (ice cold). Dispense 100 mL aliquots into pre-chilled 1.5 ml tubes.

Add about 1 µg of the desired plasmid DNA (pure or crude) to the cells in each tube.

In India many medicinal plants traditionally used by tribal and other sources have been recorded by different authors, workers and researchers. Many plants that can cause symptomatic relief for various ailments have also been established. Attempts have been made by different scientists to ascertain the active principles behind the herbal cure of certain diseases. Ayurvedic preparations from plant sources have been developed in our country to certain extent. Many Homeopathic medicines have also been procured from plants and herbs. Scientific conferences in State, National and International levels are being held on Traditional Asian Medicines to ascertain the scientific truth behind them. Council of Scientific and Industrial Research, New Delhi, Regional Research Laboratory, Bhubaneswar and many other Institutes of our country are doing creditable work to isolate the composition of medicinal plants. Many useful compounds have been isolated from different medicinal flora and their efficiency has been established scientifically. Good numbers of Allopathic medicines prepared on the basis of herbal cures are flooding in the market in different trade marks. Still the country is to find preventive and curative medicines for Cancer, AIDS, Thalsamia and other such deadly diseases. Recent International Conference on Cancer held at New Delhi came out with a suggestion that certain flora could be explored for the possibility of treating cancer without side effects. When we are starting to realize the importance of flora for a healthy living and scientists are in search of drugs from the vegetation to irradicate diseases deforestation is reampant in our country. Our environment has been contaminated due to various types of pollutants from industries and other anthropogenic sources. At this juncture man has to maintain a balance to achieve harmony of both the factors for our better living. The tribal dweller who is considered as the ‘Maa in the forest’ has to be recognized first so far his knowledge in medicinal plants is concerned. After ascertaining the utility of medicinal plants they are to be protected, propagated both by Government and Public. Scientific experiments are to be carried out to establish the active principles behind the herbal drugs. Herbal gardens are to be instituted at different places by adopting modern biotechnological methods. There is a need to make joint efforts by the workers from the fields of Allopathic, Ayurved and Homeopathic, as a step towards “the irradication of diseases”. Such an inter-disciplinary work on medicinal plants may throw a new light for achieving success, in finding out medicines without side effect. In this context, Pharmacologists have to play a vital role while preparing the doses of drugs from plant sources.

Introduction

Structural changes in host cells by the viral infection is referred as cytopathic effect (CPE) and the responsible virus is called cytopathogenic virus. As a virus hijacks a cell to make copies of itself, the cell goes through distinct changes in its chemistry and structure. These effects are easiest to see in lab grown cell cultures. The severity of the damage caused by the virus depends on the specific virus itself, the type of cell it infects, how many viruses infect each cell (MOI), and other factors.

The early stages of this damage, called cytopathic effects (CPE), can be spotted directly under a microscope without any dyes or special treatments. While various types of CPE exist in living cells, some signs of viral infection, like inclusion bodies, require staining the cells to be visible. We can classify viruses based on how quickly they cause visible damage (cytopathic effect) in cell cultures. Viruses are typically considered slow if it takes 4-5 days to see this effect in cultures with a low starting dose of virus (MOI). On the other hand, viruses are considered fast if the damage shows up in just 1-2 days under the same conditions. It is important to note that at a high MOI all CPE can occur rapidly. So all decisions about rate of CPE appearance should be based on the lowest MOI that produces CPE

Principle
Pickle is an edible product preserved and flavoured in a solution of common salt and vinegar. Spices and oil are also added. They add to palatability of a meal, aid in digestion and are good appetizers too. Pickles are prepared from fruit and vegetables like cauliflower, reddish, carrot, green chili, turnip, cabbage, onion, mango, jackfruit, aonla and lemon etc. Commercial preservation of many pickle relied upon conversion of fermentable carbohydrates by lactic acid bacteria to organic acids during bulk storage and/or the addition of sufficient amount of sugar, vinegar and other ingredients to the fully cured and packed products to preclude any microbial growth. Organic acids, oil, salt and spices all have antimicrobial properties at suitable concentration especially in combination which preserves the pickles. Pickles in brine such as or after fermentation however, need some amount of preservative or pasteurization to prevent the spoilage.

Methods in Seed Blending

For good seed blending there are three methods and details of which given as below:

Materials required
Material : Wheat Flour (1000g), Shortening (350 g), Fine Sugar (400 g), Sodium bicarbonate (10 g), Dextrose solution (100 ml) i.e 25 g Dextrose hydrate in 500 ml water, Distilled water (50 ml), salt (8 g).
Equipments: Mixer, rolling pin, Tray, cookies cutter, baking oven etc.
Principle: Cookies are made from soft wheat are having high sugar and fat content. They contain relatively low moisture content and have fairy high shelf life. Biscuits are similar name used in Europe and UK. Cookies are chemically leavened. Sugar and shortening are creamed to form dough with flour and leavening agents. When cookies dough enters the oven, the fat starts melting and sugar is dissolved which allow the cookies to spread as a function of gravity. The leavening agents expand it in all direction and structure is stabilized with high temperature prevailing in the oven.

Hazelnut is also known as a cob nut or filbert nut according to species. The hazelnut was native to the Black Sea coast long before our era, where it was found growing wild. Turkey is one of the few countries in the world having a favourable climate for hazelnut production. Hazelnuts are extensively used in confectionery to make praline and also used in combination with chocolate for chocolate truffles and products such as Nutella. Hazelnut oil recovered from hazelnuts is strongly flavoured and used as cooking oil. Hazelnuts are a good source of energy with 60.5 per cent fat content. Hazelnuts have been ranked as one of the most nutritious nuts because they supply significant amounts of protein and fiber, vitamin E and B, minerals like iron, zinc, calcium, magnesium and potassium. 

Mechanical damage is inflicted on seeds during harvesting, threshing drying, processing and handling. Injury may occur from any kind of physical abuse. Threshing abuse probably is most serious but, seed conditioning and handling also contribute to seed injury. High temperatures during drying or drying too quick or excessively can cause injury. Susceptibility to mechanical injury increases as the moisture content of the seed decreases. Effect of all individual impacts received by the seed appears to be cumulative in total seed damage. Cereals are largely immune to mechanical injury presumably because of the protective outer structures, the palea and lemma. Large sized legumes are more suscepitable. Small seeds tend to escape injury during harvest and seeds that are spherical tend to suffer less damage than elongated or irregularly shaped ones.

2. 1. Plant domestication

Process of bringing wild species under human management.

2.1.1. Changes in plant species under domestication

Elimination or reduction in shattering of pods, spikes, etc.

Elimination of dormancy

Decrease in toxins of other undesirable substances. Eg. Bitter principle of cucurbits.

Cultivated plants show altered tillering, branching, leaf characters.

Decrease in plant height. Eg. Cereals and millets.

12.1. Origin and distribution

• Indigenous to West Indies (Jamaica), Central and South America. Jamaica is the main producer of allspice.

• In India, there are few trees in Maharashtra, Tamil Nadu, Karnataka and Kerala.

• Available in the Mahendragiri hills of Kanniyakumari, and Kallar and Burliar of the Nilgiris of Tamil Nadu; Ambalavayal in Kerala.

12.2. Economic importance

• It combines the odour and flavour of nutmeg, clove, cinnamon and pepper and hence, called all spice.

• Berry, berry oil, oleoresin, leaf oil are the products of economic use.

• Used in food industry (65 to 70%), domestic use (5 to 10%), production of berry oil (20 to 25%), extraction of oleoresin (1 to 2%), and pharmaceutical and perfume industry.

• Has, anti-microbial, insecticidal, nematicidal, anti-oxidant and deodorant properties.

As the chill of winter fades away, and nature begins to bloom, it’s time to embrace the joys of spring with your furry friend by your side. While spring brings about a sense of renewal and excitement, it also brings some unique challenges for pet owners. Here are some essential tips to ensure your pet dog stays happy, healthy, and safe during the spring season:

1. Regular Grooming Sessions: Springtime means shedding season for many dogs, as they lose their winter coats to make way for lighter ones. Regular grooming sessions, including brushing and bathing, can help reduce shedding and prevent matting. Additionally, grooming helps in checking for any ticks, fleas, or skin irritations that may arise as the weather warms up.

2. Watch Out for Allergies: Just like humans, dogs can suffer from seasonal allergies too. Keep an eye out for signs such as excessive scratching, licking, or sneezing. Consult your veterinarian if you suspect your dog is experiencing allergies, as they can recommend appropriate treatment options or medication.

3. Protect Against Fleas and Ticks: Springtime brings a surge in flea and tick activity. Ensure your dog is up to date on flea and tick prevention medications recommended by your veterinarian. Regularly inspect your pet for any signs of these pests, especially after outdoor activities in grassy or wooded areas.

4. Stay Hydrated: Warmer weather means increased activity levels for both you and your dog. Make sure your pet has access to clean and fresh water at all times, especially during outdoor excursions. Dehydration can be a serious concern, so carry water and a portable bowl when going for walks or hikes.

Materials required
Material : Wheat Flour (550 g), Pearl millet Flour (50 g), Ragi Flour (50 g), Finger millet Flour (50 g), Barley Flour (50 g), Sorghum Flour (50 g), Foxtel Millet Flour (50 g), Maize Flour (50 g), Oat Flour (50 g), Besan (50 g) Shortening (350 g), Fine Sugar (400 g), Sodium bicarbonate (10 g), Distilled water (50 ml), salt (8 g). Equipments: Mixer, rolling pin, Tray, cookies cutter, baking oven etc. Principle: Cookies are made from soft wheat are having high sugar and fat content. They contain relatively low moisture content and have fairy high shelf life. Biscuits are similar name used in Europe and UK. Cookies are chemically leavened. Sugar and shortening are creamed to form dough with flour and leavening agents. When cookies dough enters the oven, the fat starts melting and sugar is dissolved which allow the cookies to spread as a function of gravity. The leavening agents expand it in all direction and structure is stabilized with high temperature prevailing in the oven.

Jackfruit is believed to be indigenous to the rain forests of the Western Ghats of India and the name “Jack fruit” is derived from the Portuguese word Jaca, which in turn, is derived from the Malayalam language term, Chakka. The jackfruit has played a significant role in Indian agriculture for centuries. Archeological findings in India have revealed that jackfruit was cultivated in India 3000 to 6000 years ago. During the season, each tree bears as many as 250 large fruits, supposed to be the largest tree-borne fruit in the world. The fruit varies widely in size, weigh from 3 to 30 kg and has oblong or round shape measuring 10 cm to 60 cm in length and above 25 to 75 cm in diameter. 

I. FILL UPS

1. Seed village is also known as _____.
2. ITC with Zeneca stands for _____.
3. ITC Zeneca has recently changed name to _____.
4. CENSAR stands for _____.
5. _____ is a compact area approach in which the whole village involved in seed production.
6. The concept of seed village is a _____.
7. Seed village concept was started in_____.
8. _____ has guided the experiment in wheat.
9. _____ is a milestone in green revolution.
10. CAPART stands for _____.

A student investigator confronted time constraints, such as course credit pressure, and resource constraints, such as physical, budgetary, and managerial constraints, in the current investigation. However, every effort and consideration made the study as objective and scientific as feasible. Despite the researcher’s best efforts, the study depends on the respondents’ opinions, which may or may not be devoid of their perceptions and biases.

As a result, it is a modest piece of labour that may be completed over a more extended period.

Suggestions for Future Research

• The findings of the present study warrant the need for further investigations in several directions. The following suggestions are made for further research in the following areas.

• The study was confined to one state; therefore, the results cannot be generalised to the whole country. Similar analyses can be taken up in other states to generalise the results.

As the temperature drops and snowflakes begin to fall, it’s essential to ensure that your beloved canine companion stays warm, healthy, and happy during the winter months. While winter brings its own set of challenges for pet owners, with some extra care and attention, you can make sure your pet thrives even in the coldest of seasons. Here are some essential tips for caring for and managing your pet dog during winter:

1. Provide Adequate Shelter: If your dog spends time outdoors, make sure they have a warm and dry shelter to retreat to when the weather gets harsh. Whether it’s a sturdy doghouse or a cozy insulated shelter, ensure it provides protection from wind, rain, snow, and low temperatures. Consider adding blankets or straw bedding for extra warmth.

2. Limit Outdoor Exposure: While dogs may enjoy playing in the snow, it’s crucial to limit their time outdoors during extreme cold weather. Frostbite and hypothermia are real dangers for dogs, especially those with short fur or small breeds. Keep outdoor activities short and supervise your dog closely to prevent any weather-related emergencies.

3. Protect Their Paws: Snow, ice, and road salt can be harsh on your dog’s paws, causing irritation, dryness, and even chemical burns. Invest in a pair of dog booties to protect their paws during walks or consider using paw balms to moisturize and create a protective barrier. After walks, wipe your dog’s paws with a warm, damp cloth to remove any salt or ice-melting chemicals.

Procedure
1. Mix the dough with ingredients I. Keep it for fermentation till breaks easily without stretching.
2. Put the sponge to mixer. Add salt, sugar, flour and optional ingredients and mix. In last, add shortening. The dough should be soft and very smooth.
3. Let the dough relax for about 30 min. cut pieces of dough weighing 100 g/450g.
4. Mould in along oblong shape and place in baking pans.
5. Proof the moulded dough to a desired height.
6. Bake at 210oC to a golden colour.
7. Cool the loaves overnight.
8. Cut the rusk of desired thickness. Arrange on baking sheets and toast at 190oC till these are crisp and golden brown in colour.
9. Cool and packaged.

Jamun is tall evergreen tree indigenous to India.  According to Hindu tradition, Rama subsisted on the fruit in the forest for 14 years during his exile from Ajodhya, because of this, many Hindus regard jamun as a ‘Fruit of the Gods’. It is known by different names in different regions of India: these names are java plum, black plum, jambul and Indian blackberry. Though the fruits of jamun are liked by all and sell at a good price, but it has still not grown as an orchard tree and is generally known as an avenue tree or as a wind break. Jamun is a hardy fruit plant which can be grown under adverse soil and climatic conditions. It is one of the hardiest fruit tree and best suited for wastelands. It is drought tolerant and at the same time can tolerate water stagnation as well as marshy wetlands. 

Mass selection is the simplest, oldest and least expensive method of plant breeding as applicable to cross-pollinated crops. The method involves planting balanced bulk seed of the population to be improved, in isolation, inferior plants rogued out before pollination wherever possible and selecting and bulking superior genotypes that already exist in the population. A balanced bulk of selected ears, for example, in corn is made for planting the next cycle. Selection units are individual plants. Selection is surely on phenotypic performance. This method exploits additive gene effects plus additive x additive interactions. The efficiency of this method depends largely on the heritability of the trait under selection. Selection results using mass selection have been quite effective for less complex traits. Mass selection could be very effective for those traits that can be identified or selected prior to flowering, for example, curd/head shape, size, colour in cauliflower and cabbage, root shape, size, colour and quality traits in radish and carrot and bulb shape, size, colour and weight in onion. A few examples of such traits in maize could be flowering, leaf angle, prolificacy, resistance to thrips, fall armyworm, reduced plant and ear height, reduced anthesis-silking interval and barrenness and tolerance to pre-flowering diseases.

Lack of pollination control is major limitation of this method as both desirable and undesirable pollen will be involved in pollination of the selected plants. Further, if selection intensity is very high (small plant population size advanced), the possibility of inbreeding depression is increased. This selection is not very effective if the heritability of the trait under selection is low (less than 10% on individual plant basis). Mass selection is likely to be more effective for individual or a combination of few traits rather than a whole series of characters. Despite some limitations, mass selection can be very useful and relevant procedure in breeding of cross-pollinated crops in many situations characterized by resource limitations, non-availability of trained scientific manpower and additional test sites, lack of seed storage facilities even for short term and lack of continuity of technical personnel. Lack of control on soil variability also makes this method less effective.