eChapters

12.1. Origin and distribution

• Native of Persia and neighbouring tracts of Western and Southern Asia

• In India, North Guj arat, Raj asthan, Haryana and Bihar are the maj or states.

• Husk and peel - exported to USA, Germany, UK and France

12.2. Economic importance

• Psyllium from Greek meaning flea, in reference to colour, size and shape.

• Economic part - Seed coat, husk used in indigenous medicine. Seeds contain glucoside and tannin.

• Works as an anti-diarrhoea drug.

• Seed - Pentose, a sugar is isolated.

Date:

Objective: To write a radio script for infotainment program.

Overview: Radio is an electronic audio medium that can broadcast messages to a large number of dispersed audience. Radio has several advantages as a medium of communication, especially in rural areas:

a) Radio can be carried around.

b) Low cost of production and ownership.

c) Usage is not limited by literacy and technical know-how.

d) It can be used by the listener while doing other tasks.

e) Usage is not limited by power supply.

f) Easy to operate.

Objective: The order Dictyoptera includes insects like cockroaches and praying mantises with netted wings. Recognizing their unique taxonomic traits is essential for precise identification and classification.

A high-yielding cultivar Vallabh Medha of mandukparni (Centella asiatica) was identified. Fresh herbage yield (12,331 kg/ha) and dry herbage yield (2,113 kg/ ha) of this variety were far more than the local variety (2,050 and 392 kg/ha respectively), besides higher active ingredients. Among mucuna selections, IIHR PS 15 with long duration, IIHR PS 6 with medium duration and IIHR PS 14 with short duration recorded significantly higher yield and high L-dopa yield/ plant. In Coleus, a promising hybrid, Hy 08-53, recorded significantly higher root yield (60.22g) and higher forskolin yield/plant (0.58 g). In ashwagandha, IIHR- WS 3 was found to be the best yielder (11.65 q/ha).

Family: Amaranthaceae

Flowering: November-December

Fruiting: January-March

Botanical description: The plant is a herb, reaches heights of over 1 meter tall with fleshy oval shaped leaves that are sometimes pointed at the tips. It is having a deep maroon colour in the center of the leaf with streaks of red, purple and green. The plant produces feathery purple, magenta or red flowers from the central stalk which is packed with edible seeds. The flower buds are edible, once they mature and become bushy.

Ecology: The plant is commonly used as leafy vegetables and cultivated throughout the country. The plant loves to grow in the sandy soil in winter season. It is also cultivated in left over potato fields.

Use: Leaves and young shoots are cut into small pieces, cooked with salt and chilly and then eaten. Sometimes the leaves and shoots are mixed with vegetables in cooking.

1.    Collect the bone marrow from iliac crest of rabbit, dog, goat and sheep; sternum of pigs by 16-18 G bone marrow biopsy needle under sedation/anesthesia and maintain strict asepsis protocol
2.    To aspirate bone marrow, negative pressure should create in the syringe by pulling back the plunger. Syringe should be first flushed with heparin before bone marrow collection
3.    Collect approximately 2.5-5 ml of bone marrow from each animal
4.    Mix the bone marrow sample with equal volume of low DMEM having 10% FBS and 1 ml of PS and then layer on 5 ml of histopaque/Ficol-Paque Plus in a falcon tube
5.    Centrifuge at 3000 rpm for 30 min
6.    Collect stem cells from the interface with the help of a micropipette
7.    Cells wash thrice with DPBS 
8.    Centrifuged at 2000 rpm for 5 min

Refers to induced chromosome manipulation

Aneuploidy-One or few chromosomes extra or missing from 2n.

Autoploidy-Genomes identical with each other.

Aim : To determine the Calcium in plant sample.

Date:

Objective: To understand basics of photography and practice taking good quality photographs.

Overview: Photograph refers to any image which is created when light falls on a photosensitive surface. With the advent of mobile phones and decreasing cost of technology, many people are taking up photography as a hobby or profession. Photographs are very useful in communication, extension and education, as they arouse interest, simplify complex ideas/ things, can be used to convey messages to illiterate people, and enhance credibility of the information. Apart from technical expertise, it is important to know the basics of composition to take good photographs.

Objective: To investigate and comprehend the taxonomic traits of family Mantidae, specifically cockroaches and praying mantises, through detailed examination and diagrammatic representation.

Family: Amaranthaceae

Flowering: June-August

Fruiting: September-November

Botanical description: The plant is an annual erect herb that grows upto 1 meter in height. The stem is grooved and often redish. The leaves are long-petioled, ovate or elliptic, acute or subacute with base shortly cuneate to attenuate. The flowers are in axillary and terminal red or green panicled spikes with up to 20 cm long. Seeds are black in colour, shining and compressed or subspherical with thick margin.

Ecology: The plant being commonly used as leafy vegetables, is cultivated throughout the country. The plant loves to grow in soil rich with organic materials. The plant is commonly cultivated in summer and it grows luxuriantly in rainy season.

1.    Remove the growth media
2.    Wash 2 times with warm PBS (w/o Ca and Mg)
3.    Add 5ml of trypsin /EDTA 0.35 % (Invitrogen), place at 370C in a CO2 incubator for 4-5 min
4.    Wait until cells are detached/lifted and make sure by examining on microscope
5.    Add equal volume (5ml-1:1) of growth medium as appropriate and mix with 5 strokes
6.    Transfer into 15 ml centrifuge tube
7.    Centrifuge at room temperature for 4 min in 980 rpm
8.    Remove supernatant complete but carefully without touching the cell aliquot
9.    Re-suspend in 10ml DPBS (with Ca and Mg)-5 strokes
10.    Immediately after mixing transfer 100µl of cell suspension into Casy vial, containing 10ml Casyton

Aim : To determine the Magnesium in plant sample.

14.1. Origin and distribution

• Originated from Africa and distributed throughout warmer regions of world

• Naturally grown in hilly areas of Gujarat, Rajasthan and Madhya Pradesh

14.2. Economic importance

• Economic part - Root

• Roots of safed musli contains 2-15% saponin having medicinal property of enhancing vitality and immunity to human beings.

• Also helps in correcting gynecological disorders.

• Dried tubers - Used as curative for pre-natal and post-natal illness, arthritis, restorative and a health tonic

• Known as divya aushadhi and ayurvedic anori.

Date:

Objective: To visit a video production facility to understand the process and set up.

Overview: Video programs are often produced in a studio environment as it is convenient, easy to manage and there are very few disruptions. A typical video production facility comprises of two areas – control room and the studio floor. Director, assistant director, video and audio technicians sit in the control room. The control room has a series of monitors that display the final and camera outputs. It may also have “on air” and “preview” monitors which display images being broadcast and to check the image before recording, respectively. The control room also has a production console, which is used to select the video and audio output.

Key Points

• Allergies are widespread among animals.

• Common allergens include food and drugs.

• Allergic reactions can occur through inhalation, skin contact, or eating.

• Housed cattle develop hypersensitivity pneumonitis, a persistent respiratory illness.

• Chronic exposure to spores in moldy, dusty hay is linked to the disease.

• There are two types of hypersensitivity: acute (also known as anaphylaxis or shock) and delayed.

Objective: To explore and document the taxonomic characteristics of cockroaches belonging to the family Blattidae through detailed observation and analysis.

Family: Amaranthaceae

Flowering: June-August

Fruiting: September-December

Botanical description: The plant is an annual herb with sometimes red tinged erect stems, sometimes ascending, 30-150 cm long, usually branched. The leaves are ovate to rhombic- ovate, elliptic, lanceolate-oblong, or lanceolate, blades 1-12 cm long, 0.89-6 cm wide and smooth. The stem and its branches carry spines. Flowers of the plant are green, and are in axillary clusters in the lower part of the plant and in unbranched or branched spikes in the upper part. The lower clusters are entirely without stamens as the lower flowers of the spikes, the upper flowers in the spikes are staminate. Seeds are dark brown or black in colour.

Date:

Objective: To understand shot sizes in a video production.

Overview: Shot, in video production refers to one continuous recording by the camera. It comprises of a series of frames (still images) and There are 24 frames in one second. A shot includes eeverything recorded between switching on and switching off the record button on the camera. It can contain movement of both camera and talent.

Acetylcholinesterase activity in brain is measured by the method described by Ellman’s et al. (1961).

Reagents

1. Phosphate buffer (0.1M) (pH 8.0) Solution A: 5.22 g of K2 HPO4 and 4.68 g of NaH2 PO4 are dissolved in 150 ml of distilled water. Solution B: 6.2 g of NaOH is dissolved in 150 ml of distilled water. Solution B is added to solution A to get desire pH 8.0 and then finally the volume is made up to 300 ml with distilled water.

2. DTNB reagent 39.6 mg of DTNB with 15 mg of NaHCO3 is dissolved in 10 ml of 0.1 M phosphate buffer.

3. Acetylthiocholine (ATC) 21.67 mg of Acetylthiocholine is dissolved in 1 ml of distilled water.

Procedure

The brain tissue is weighed and 10% homogenate is made in 0.1M Phosphate buffer (pH 8.0). Take 0.4 ml aliquot of homogenate in a cuvette containing 2.6 ml phosphate buffer (0.1M) and 100 µl DTNB. The contents of the cuvette are mixed thoroughly by bubbling air and absorbance is measured at 412 nm in a spectrophotometer. When absorbance reached a stable value, it is recorded as the basal reading .To this 20 µl of acetylthiocholine is added and change in absorbance is recorded for a period of 10 min at intervals of 2 min. Change in the absorbance per minute determined.