eChapters

Fruit crop productivity is greatly impacted by rootstocks in a number of areas, including canopy structure, fruit quality, yield, blooming, and nutritional absorption. Furthermore, they are an essential line of defence against biotic and abiotic stressors such salt, heat stress, soil pathogens, and nutritional imbalances. Because there is a shortage of arable land and a growing market for fruit crops, production frequently takes place in unfavourable environments with high temperatures, little precipitation, floods, salt, and exposure to organic contaminants. The strategic use of appropriate rootstocks is crucial to reducing output losses under these conditions because they can lessen the effect of outside pressures on the scion (Bhandari & Bhattacharya, 2019) Rootstocks are essential to orchard management because they provide anchoring and facilitate the uptake of water and minerals. They also affect the growth of the trees, and they help create “three-piece” trees when they are employed as interstems, placed between the scion and the rootstock during propagation. Interstems assist regulate excessive vigour in desirable rootstocks and handle compatibility difficulties between stock and scion (Singh & Kumar, 2021).
Soils that are too dry or too moist can be made tolerable by rootstocks. The potential of rootstocks in commercial fruit crops has not yet been completely utilised in India, despite substantial research internationally on the discovery and use of appropriate rootstocks in crops including apple, pear, citrus, mango, and grapes. Therefore, the crucial next step involves identifying rootstocks with optimal characteristics tailored to specific environmental conditions, acknowledging that the best rootstock for one variety and set of conditions may not be suitable for another (Sahu & Patra, 2018).
Presently seedlings from open-pollinated seeds are used to propagate guava instead of homogenous clonal rootstocks. It was determined that many rootstocks, such as P. cattleianum, P. guinesee, P. molle, and Philippine guava, were appropriate. Plants using P. cattleianum rootstock had the best yield and the tallest trees. P. pumilum produced fruits with the largest total sugar content, total soluble solids (TSS), and quantity of seeds possible, exhibiting a dwarfing effect. Despite having uneven and rough skin, P. cujavillis rootstock produced

It is not possible that every respondent is willing to and capable of giving honest and true response. Sometimes, it is practically not possible to make the respondent reveal about personal information, but true research requires true response. When direct stimuli fail to elicit true response, then we must use indirect and extractive methods to elicit the required response indirectly from the respondents, which further can be interpreted in the relevant format. This indirect and ambiguous stimulus based methods are known as projective methods. Projective methods are technique of data collection on the person, value, attitude, emotions and motives etc. by using a highly unstructured and ambiguous stimulus (Wilkening, Wilkening & Wilkening, 1973).

Family: Caesalpiniaceae

Flowering: April-June

Fruiting: December-March

Botanical description: The plant is a climber growing up to 10-30 m long. The woody stem can get as thick as 20 cm. The spreading tout branches are covered with rusty fine hairs. The stout tendrils are coiling and occur in pairs. Large leaves are 10-45 cm, 2-lobed with a broad cut. The flowers are white, 2-3 cm across, turn yellow when old and borne in rounded clusters. Fruit is a flat woody pod with fine rusty hairs, 20-30 cm long.

The lymph from the fore limb is collected and carried into the circulation through two main lymph nodes, the prescapular and the axillary lymph nodes. There may be two more lymph nodes in fore limb but those are inconsistent and only occasionally found in ox. These include the accessory axillary lymph node and infraspinous lymph nodes and their afferents are mostly from the thoracic wall.

24.1. Origin and distribution

• Originated in hills of South Western India, Indo-Malaya region.

24.2. Economic importance

• Used to improve appetite and digestion and treat stomachache, heart burn, indigestion, intestinal gas, diarrhoea and cholera.

• Fruits are stomachic and carminative.

• Used for lung problems including Asthma, bronchitis and cough.

24.3. Varieties

• Vellanikara -1 (Visvum tippili) and Yercaud (Pl) - 9.

24.4. Climate

• Elevation between 100 and 1000 m.

• Shade intensity: 20-25%.

• Lower elevations with high humidity

Objective: To identify and understand the taxonomic status and relationships of orders within Endopterygota, focusing on their distinct larval and adult stages and current sister group hypotheses.

Data collected by using different data collection methods remain meaningless until it is screened, cleaned, coded, analyzed and summarized. Data cleaning and screening mostly come under the purview of statistical logics and techniques hence not dealt with in this practical guide. Since these aspects will be dealt by the statistical experts from the allied statistical wings. Presuming that students are aware about data cleaning and screening, we will proceed further with data coding.

Family: Caesalpiniaceae

Flowering: February-March

Fruiting: April-May

Botanical description: The plant is a moderate sized tree growing up to 20-40 ft tall and 10-20 ft wide with a spreading crown of briefly deciduous leaves which are 10-15 cm across and rounded with lobed ends and heart shaped bases. The leaves are shaped like a cow’s hoof. The flowers are white or purple in colour with one petal variegated yellow, 5-10 cm across and carried in clusters at the branch tips. The flowers often make their first appearance in late winter while the tree is bare of leaves. Seeds of the plant are sub-orbicular in shape.

25.1. Origin and distribution

• Native to Western and South Western Europe, Western and Central Asia and Erstwhile USSR countries.

25.2. Economic importance

• Dried leaves constitute the drug, digitoxin and gitoxin.

• Used to treat congestive heart failure and heart rhythm problems.

• Increase blood flow throughout the body and reduce swelling in hands and ankles.

25.3. Varieties

• Strain E.C. 115996 - Higher foliage yield with high glycoside content

25.4. Climate

• Requires a cool and mild climate (20-30°C)

• Well in hill slopes at elevation of above 1250 m above msl

25.5. Soil

• Well drained sandy soil rich in organic matter and slightly acidic (pH 5.5-6.5)

Objective: To explore and document the taxonomic characteristics of Strepsiptera and Mecoptera, highlighting their unique morphological features and biological adaptations.

Endometritis is one of the leading causes of reproductive inefficiency, infertility and decreased milk yield in high yielding dairy cows and buffaloes as well as in other species of animals and are associate with severe economic loss. Indiscriminate use of antimicrobial agents has resulted in development of resistance to the available antimicrobials. Plant-derived active principles have promising potential to act as an alternative to conventional antimicrobial agents. In the present study, an attempt was made to investigate the mechanism of action of Eucalyptus robusta leaves (ER) extract against resistant bacterial isolates. Out of 70 clinical samples of uterine discharges from cattle and buffaloes having history of uterine infection, we have identified one isolate as methicillin-resistant Staphylococcus. As per the CLSI guideline, this isolate (S4) was found to be catalase +ve, oxidase –ve and possess mecA and coaA genes along with shown resistance to cefoxitin and MIC breakpoint for oxacillin was >4 µg/ml. Methanolic extract of ER reconstituted in water showed presence of eucalyptol (marker compound) along with other major bioactive phytoconstituents following GC-MS analysis. ER extract exhibited marked in vitro antibacterial effect against S4 isolate and MRSA (ATCC 33591) as evidenced by agar well diffusion test with MIC value of 0.3125 mg/ml.

Within a few weeks after November 8, 1895, Wilhelm Conrad Roentgen made the discovery of X-rays, they were being used in medicine and many sophisticated medical applications. This single discovery of X-rays revolutionized the entire medical field and developed into a separate discipline in the diagnostic and therapeutic applications in human beings and animals. On 10 December, 1901, Roentgen received the first Nobel Prize for Physics in recognition for discovery of X-rays. Radiography in veterinary practice may not always be used to arrive at a final diagnosis of the disorders. In the last few years, various types of X-ray scanners have been developed that allow highly detailed views of a particular section of the body. One type, known as a computerized tomography (CT) scanner, sends narrow beams of X- rays at various angles through a patient’s body. The information obtained from the X-rays is processed by a computer to produce an image of a cross-section of the body. The image shows much more detail than an ordinary X-ray picture. A section of the body can be studied in three dimensions by producing a series of adjacent cross-sectional images. Nevertheless, radiography remains a valuable modality in diagnostic purposes and has significantly extended the usefulness in medicine and research.

Bioinformatics is a multidisciplinary approach that combines computational and biological expertise to analyze biological data to advance research and development and it drew public attention with the announcement of the draft sequence of human genome by the then US President, Bill Clinton along with Craig Venter and Francis Collins in 2000. Bioinformatics and life sciences research are gaining attention in the government, industries and academic sectors. India was the first country in the world to establish a biotechnology information system (BTIS) network in 1987 through the Dept. of Biotechnology and created an infrastructure that enables it to harness biotechnology through the application of bioinformatics. Through this branch of science, biological data are acquired, stored and then analyzed. Bioinformatics can be used to store huge bioscience data for their future uses. By exploring computational tools, databases and new strategies for pests and diseases, resistant genes can be identified and plants with desired traits can be engineered to enhance agricultural productivity. Bioinformatics can be utilized in two ways for agriculture, viz., (i) at molecular level for application in breeding strategies and (ii) agri-informatics for land use planning, farmers window, early warning system, impact assessment and environmental applications.

WNV was first identified in the West Nile district of Uganda in 1937. Reported in Western Hemisphere for the first time in 1999, in New York City. The virus had until that time been found only in Africa, Eastern Europe, West Asia and the Middle East.

Family: Cucurbitaceae

Flowering: October-November

Fruiting: December-January

Botanical description: The plant is a annual climbing herb, having stems up to 6 metres long that scramble over the ground or climb on other plants, supporting themselves by means of tendrils. Leaves are roundish, kidney-shaped, base deeply heart-shaped. Upper surface is rough, lower surface shortly bristly, 5-7-lobed, with long and hairy stalk. Tendrils are slender and rarely simple. Flowers are yellow in colour, calyx tube 10-15 mm long with spreading petals ending in a short point. Fruits large, succulent, densely hairy when young, with a thick waxy deposit when mature.

26.1. Origin

• Native to Dalmatia (Yugoslavia), Herzegovina and Montenegro.

26.1. Economic importance

• Natural insecticide from dried flower heads.

• Seed or achene contains Pyrethrin I, II, Cinerin I, II, Jasmolin I, II collectively called Pyrethrins.

26.2. Varieties and types

• KKL-1 released from TNAU.

• Hansa and Jhelum released from CIMAP, Lucknow.

• Sel-2 - developed at the Kodaikanal Centre of CIMAP.

• Two types

? Dalmatian pyrethrum: C. cinerariaefolium, grown on the Easte Coast of the Adriatic Sea.

? Japanese pyrethrum also derived from C. cinerariaefolium.

Objective: To study and document the taxonomic characteristics of Siphonaptera and Trichoptera, emphasizing their unique morphological features and ecological adaptations.

1. The removal of moister from the timber is known as seasoning.

2. For boat building purpose timber should have moisture about 18-20%.

3. Mild steel contain 0.15-0.30% of carbon.

4. Low carbon steel contain less than 0.15% of carbon.

5. High carbon steel contain 0.8-1.5% of carbon.

6. The specific gravity of steel 7.84.

7. The aluminum boat was firstly built in France 1892.

8. Ferro cement boat was introduced in France 1847.

9. Specific gravity of aluminum-2.7.

10. Specific gravity of Ferro cement 2.4-2.6.

This chapter introduces hybridoma technology and its application in producing monoclonal antibodies. It explains the fusion of antibody-producing B lymphocytes with immortal myeloma cells, selection in HAT medium and the roles of HGPRT and polyethylene glycol. The survival and cloning of hybrid cells and the advantages of monoclonal antibodies are considered. The chapter also discusses tissue and organ transplantation, including autografts, isografts, allografts and xenografts. The immunological mechanisms of acute graft rejection, donor histocompatibility antigens and the involvement of T lymphocytes are addressed. These questions connect laboratory antibody production with transplantation immunology and its diagnostic, therapeutic and research applications.