eChapters

1    Introduction to Patent Laws    540

2.    An Overview of the Patenting Process    541

    2.1.    Inventor’s disclosure, 542
    2.2.    Patentability search, 542
    2.3.    Patent application preparation, 542
    2.4.    Patent application amendment, 542
    2.5.    Patent application examination, 543
    2.6    Main parts of a patent, 543
    2.7.    Granting of patent, 544
    2.8.    Date and term of patent, 544
    2.9.    Provisional specification, 544
    2.10.    Complete specification, 545
    2.11.      Cost of patenting, 545

1.    Introduction    496

2.    Strain Development Technologies    496

    2.1.    Screening of natural variants of microorganisms, 496
    2.2.    Genetic manipulation of microorganisms, 498
        2.2.1.    Random mutagenesis and selection, 498
        2.2.2.    Conjugation, 504
        2.2.3.    Transduction, 504
        2.2.4.    Transposition, 506
        2.2.5.      Hybridization, 507
        2.2.6.    Rare Mating, 509
        2.2.7.    Cytoduction, 510
        2.2.8.    Protoplast fusion, 511
        2.2.9.    Transformation, 511
        2.2.10.    Gene cloning and transformation (Recombinant DNA technology), 513

1.    Introduction    468

2.    Biofuel Cell    470

    2.1.    Microbial-based Biofuel Cells, 471
        2.1.1.    Microbial bioreactors producing H2 for conventional fuel  cells, 475
        2.1.2.    Microbial fuel cells producing electrochemically active metabolites in  
                the anodic compartment, 476
        2.1.3.    Microbial fuel cells operating in the presence of artificial electron relays, 477
        2.1.4.    Microbial fuel cells producing electricity during wastewater treatment, 483
        2.1.5.    Microbial fuel cell producing  electricity from acetate or butyrate, 484

1.     Introduction    424

2.    Conventional Methods of Hydrogen Production    426

3.    Biological Methods of Hydrogen Production    429

    3.1.    Hydrogen production by biophotolysis of water, 430
        3.1.1. Photolysis by cyanobacteria (Blue green algae), 430
            3.1.1.1. Nitrogenase dependent hydrogen production, 434
            3.1.1.2.  Hydrogenase-dependent hydrogen production, 437
            3.1.1.3.  Factors affecting  hydrogen production by cyanobacteria, 440
            3.1.1.4.   Bioreactors for cyanobacterial hydrogen production, 443
            3.1.1.5. Future prospects for cyanobacterial hydrogen production, 446

1.    Introduction    392

2.    Landfill Gas    393

3.    Biogas    394

    3.1.    Benefits of biogas, 394
    3.2.    Potential of biogas, 395
    3.3.    Biogas production system, 396
        3.3.1.    Raw materials, 396
        3.3.2.    Biogas technology, 398
        3.3.3.    Process of anaerobic fermentation for methane generation, 400
        3.3.4.    Conditions for anaerobic fermentation, 404
        3.3.5.    Sludge-algae-methane system, 407
        3.3.6.    Biogas plant, 408

1.    Introduction    360
2.    Feedstocks for Biodiesel Production    361

    2.1.    Fats and oils, 362
    2.2.    Alcohols, 363
    2.3.    Catalysts and neutralizers, 367

1.    Introduction    302
2.    Raw Materials for Alcohol Production    304

    2.1.    Sugary substrates, 306
    2.2.    Starchy residues (cereal grains), 309
    2.3.    Cellulosic materials (crop residues), 314

3.    Microorganisms for Alcohol Production    316

    3.1.    Comparision of ethanol production by bacteria and yeasts, 318

1.    Introduction    272
2.    Alcohols    273

    2.1.    Methanol, 274
    2.2.    Ethanol, 277
    2.3.        Butanol, 283

3.    Biodiesel    284
4.    P-series fuels

1.    Introduction    232
2.    Photosynthesis and Biomass Yields    232
3.    Biomass Properties    233
    3.1.     Polymeric and chemical composition, 233
    3.2.     Fuels analysis, 235
    3.3.     Physical properties, 236

1.    Introduction    174
2.    The Thermodynamic and Mechanistic basis of Cellular Metabolism    174

    2.1.      Adenosine triphosphate, 175
    2.2.    Coenzyme A, 176
    2.3.    Modes of ATP synthesis, 177

3.      Components of Electron Transport Chain    181

    3.1.    Electron carriers in ETC, 182
    3.2.    Complex I - NADH dehydrogenase (NADH coenzyme Q reductase), 185
    3.3.    Complex II - Succinate - coenzyme Q reductase, 187
    3.4.    Complex III - Coenzyme Q - cytochrome c reductase, 187
    3.5.    Complex IV - Cytochrome c oxidase, 188
    3.6.    ATP synthese, 188

1.    Introduction    129
2.    Soil Microbiology    129

    2.1.    Bacteria, 130
    2.2.    Fungi, 132
    2.3.    Algae and Cyanobacteria, 132
    2.4.    Protozoa, 132
    2.5.    Viruses, 135

3.    Water Microbiology    133
4.    Microbial Associations    133

    4.1.    Neutral interactions, 133
    4.2.    Positive interactions, 133
    4.3.    Negative interactions, 133

1.    Introduction    71
2.    Microorganisms in Food Industry    72
    2.1.    Microorganisms as the agents of fermentation in  
        traditional fermented foods, 73
    2.2.    Microorganisms as the source of food, 83
    2.3.    Microorganisms as a source of food supplements, 86

3.    Microorganisms in Pharmaceutical Industry    92

    3.1.    Antimicrobial agents from microorganisms, 92
    3.2.     Microorganisms as probiotics, 97
    3.3.    Microbial enzymes in  pharmaceuticals, 98
    3.4.    Mushroom nutriceuticals, 98

1.    Introduction    3
2.    History and Scope of Microbiology    4
    2.1.    Controversy over the origin of microorganisms, 5
    2.2.    Germ theory of fermentation, 6
    2.3.    Germ theory of disease, 6
    2.4.    Development of laboratory techniques and pure cultures, 7
    2.5.    Discovery of immunization, 7
    2.6.    Discovery of protection against infection, 8
    2.7.    Discovery of chemotherapeutic agents, 8
    2.8.    Microorganisms as geochemical agents, 9

Aconites, the tuberous roots of which contain highly toxic alkaloids, are deadly poisons. In small doses, aconites are beneficial in nasal catarrh, sore throat, gibbous, paralysis and chronic fevers. Aconitum is a genus of perennial, erect or rarely twining herbs distributed over North temperate regions of world. The mountains of Eastern Asia form the primary centre, presenting the greatest diversity of genus. About 28 species are reported from India, mostly confined to alpine and subalpine belts of Himalayas. Of the Aconitum spp. occurring in India, roots of nine species are obtained in Indian market. In fact, aconite sold in Indian market is a mixture of 304 species. Still, A. ferox, known as Indian Aconite, is considered the most important.

A. ferox is a perennial herb with tuberous roots. Roots are paired, daughter tuber, ovoid oblong to ellipsoid, with filiform root fibres. Leaves are scattered, orbicular-cordate to reniform, palmately 5 lobed, resembling melon leaves. Petioles are pubescent. Flowers are pale dirty blue, borne in a dense terminal raceme, 10-25 cm long. Follicles are oblong with long, obovoid winged seeds.

Aloe, known for laxative property is one of the old natural remedies that have emerged as modern miracle drug. Popularly known as ‘nature’s sun screen’ has growing use as skin tonic in cosmetic industry and as a nutraceutical. Aloes are also quite popular among succulent ornamental plants because of their stiff, harsh and rugged habit and are commonly grown in deserts and other dry situations. Genus Aloe is indigenous to African continent and Mediterranean countries such as Greece and Southern Italy, and got naturalized in arid tracts of India. In India, Aloe barbadensis is cultivated on a small scale in Rajastan, Andhra Pradesh and Gujarat (CSIR, 1998).

Botany

Genus Aloe belonging to Liliaceae comprises 275 species distributed in Africa, Asia and America. Forty-two species belong to Madagascar region, 12-15 to Arabian Peninsula and rest are distributed over tropical South Africa. Aloe barbadensis, A. ferox and A. arborescens are used as herbal drugs. In India, only four species occur and of these, Aloe barbadensis is the most widely naturalized species. These taxa comprise several varieties viz., officinalis, chinensis, littoralis and they cross freely and several intermediary forms occur in semi wild state.

Good Agricultural Practices

Guidelines for Good Agricultural Practices of Medicinal and Aromatic Plants is intended to apply to the growing and primary processing of all such plants traded and used in food, feed, pharmaceutical, flavouring and perfume industries. The main aim is to ensure that the raw material meets the demands of the consumer and maintains standards of the highest purity. Important aspects are that, these are produced hygienically, in order to reduce microbial load to a minimum, and are produced with care, so that the negative impacts affecting plant during cultivation, processing and storage can be minimised. The guidelines provide guidance for producers to reduce raw material contamination to the lowest level. This document can be used in bilateral relationship between the supplier and the customer and the products documentation is regarded confidentiall (EUROPAM).

Medicinal plants and their various products are extensively utilized not only in traditional systems of medicine but also in modern medicine throughout the world. In recent times, they have also attracted attention of scientists encompassing various disciplines of medicine and biotechnology for search of new drug molecules and modern drug development. Although several research strategies are followed for drug development only the traditional, empirical and molecular methods/strategies are mostly applied. There is also need of scientific validation for safety, efficacy and rational use of traditional form of medicine along with development of standards for identity, purity and strength (Singh and Bagchi, 2002).

Approaches of Drug Developement

Following approaches are generally employed to discover and invent new therapeutic agents from plants viz. screening of plant materials, identification of active constituents, pharmacological and clinical evaluation and drug design.

Phytochemistry

Pytochemistry is concerned with enormous variety of organic substances that are elaborated and accumulated by plants and deals with chemical structures of these substances and biosynthesis, and their natural distribution and biological function. Chemical constituents of plants can be classified in different ways. Based on biosynthetic origin, solubility properties and presence of certain key functional groups, it is classified as phenolic compounds, substances that are readily recognized by their hydrophilic nature and by their common origin from aromatic precursor shikimic acid. Terpenoids share lipid properties and a biosynthetic origin from isopentenyl pyrophosphate (Fig. 1). Organic acids, lipids, alkenes and related hydrocarbons, polyacetylenes and sulphur compounds are derived biosynthetically from acetate. Nitrogen compounds of plants like amino acids, amines, alkaloids, cynogenic glycosides, indoles, purines, pyramidines and cytokinins and chlorophyll are basic substances recognized by their positive responses to either ninhydrin or Dragendorff reagent. Monosaccharides, oligosaccharides, sugar alcohols and cyclitols are water-soluble carbohydrates and their derivatives. Macromolecules of plants such as nucleic acids, proteins and polysaccharides are easily separated from other constituents by their high molecular weights (Harborne, 1984). Some of the therapeutically active compounds are discussed below:

Plants have been a major source of therapeutic agents since time immemorial. The increasing acceptance of traditional herbal systems of medicine, like Ayurveda, within India and outside has resulted in the revival of ancient traditions of medicine. Medicinal plants and their derivatives are thus looked upon not only as a source of affordable healthcare but also as an important commodity item of international trade and commerce. As per World Health Organization estimates, traditional medicines, mostly plant drugs, cater to the health needs of nearly 80% of the world population. The medicinal plant related trade is growing rapidly every year due to the growing recognition of natural products being non-narcotic, with relatively less side effects and being easily available at affordable costs.

In India, medicinal plant sector has traditionally occupied an important position in the socio-economic, cultural and spiritual arena of rural and tribal lives. About 8000 flowering plants, 650 lichens, 650 algae, 200 pteridophytes and 150 bryophytes are attributed with medicinal properties. The Indian coded systems encompasses a large number of treaties on recognized systems of medicine viz. Ayurveda, Unani and Siddha. The Ayurvedic system of medicine, which cater to the health needs of a major segment of population currently utilizes as many as 1000 single drugs and over 8000 compound formulations of recognized merit. Other systems of medicine viz. Siddha, Unani and Amchi (Tibetan) systems of medicine together utilize about 1800-1900 medicinal species. Many medicinal plants are the source of clinically useful prescription drugs being used in modern systems of medicine. Also incredible knowledge on phyto medicine is acquired in non-coded form by tribals and rural community, as is clear from evidences related to folklore medicine. Recognizing their importance, medicinal plant sector has been identified as one of the key areas by the Government of India and the Department of Indian System of Medicine and Homeopathy.

Management of paddy straw is one of the important problems being faced by the farmers. Burning of rice straw in the field is the traditional method being practiced by most of the farmers, which causes atmospheric pollution and nutrient loss. There are three options available to manage the straw viz. i) burning the straw in the field, ii) collecting/baling the straw from the field, and incorporation of straw in the field. If rice straw is not burnt then baling may provide an attractive economic environmentally safe option. Manual collection of paddy straw is a laborious job and also storing the large volume of straw is another problem. As for as paddy straw is concerned, it has higher silica content so animals do not prefer it.