eChapters

The spermatogenesis is a continuous process in male animals but affected by following factors. 
    
1.     Genetic makeup of male animal: Certain sperm defects like Diadem defect, knobbed sperm, decapitated sperm, sterilizing tail stump, corkscrew mid piece and narrow head are inherited defects and reported in certain males. Few inherited genetic factors are also associated with poor libido and poor fertility. Inherited sperm defects affect sperm motility, fertilization and embryo formation.

This vertebra is decidedly atypical in form and structure. The body and spineous process are absent. It has the form of a strong ring, from which two curved plates, the wings, project laterally. The ring incloses a very large vertebral foramen and consistis of two lateral masses connected by dorsal and ventral arches.

The lateral masses (Massae laterales) present two deep oval anterior articular cavities (Foveae articulares craniales) which receive the occipital condyles; they are separated by a wide notch above and a narrow one below. The lateral margin is also notched, and a triangular non-articular depression cuts into the medial part of each cavity. The posterior articular surfaces (Facies articulares caudales) are somewhat saddle-shaped; they are confluent on the ventral arch, but are widely separated dorsally, and do not conform in shape to the corresponding surfaces of the axis.

The dorsal arch (Arcus dorsali,) presents a median dorsal tubercle (Tuberculum dorsale) and is concave ventrally. It is perforated on either side near its anterior margin by the inter vertebral foramen (Foramen invertebrale). The anterior border is deeply notched, and the posterior border is thin and concave.

The ventral arch (Arcus ventralis) is thicker, narrower, and less curved than the dorsal. In its lower surface is the ventral tubercle (Tuberculum ventrale), into which the terminal tendon of the longus colli muscle is inserted. The upper face has posteriorly a transversely- concave articular surface, the fovea dentis, on which the dens or odontoid process of the axis rests.

In front of this is a transverse rough excavation and a ridge for the attachment of the ligamentirm dentis.

Principle

Polyethylene glycol (PEG) is a high molecular weight compound which is inert to rumen fermentation. The concentration in the rumen liquor declines with the passage of digesta from rumen to lower G.I. tract and is proportional to time. Protein free filtrate of rumen liquor gives turbidity in the presence of trichloro acetic acid and barium chloride which is a measure of PEG concentration at different post feeding hours.

Family : Moraceae

Flowering: December-January

Fruiting: June-July

Botanical Description: The plant is an evergreen tree that grows up to 20-30 meter tall and 80-200 cm in diameter with evergreen, alternate, glossy and leathery leaves reaching up to 9 inch long. Leaves are oval on mature trees. All parts of the plant contain sticky and white latex. Inflorescence is solitary, borne axillary on special lateral short leafy shoots arising from older branches. Fruits are large arising from the tree trunk and weighting about 10-50 kg. the exterior of the fruit is green or yellow when riped and composed of numerous hard, cone like points attached to a thick and rubbery, pale yellow or whitish wall.

Theory

This type of moisture measurement is called brown-duvel fractional distillation method. The method is recognized as an official method for determination of moisture content. In this method, directly measures the volume of moisture in cubic centimeter. This apparatus consists of flask, burner, thermometer, condenser, graduated cylinder. This apparatus consists of flask in which grain sample is boiled with mineral oil.

Family : Oxalidaceae

Flowering: July-September

Fruiting: October-December

Botanical Description: The plant is a small evergreen tropical tree that grows upto 25 feet in height. The compound leaves are soft, medium green, spirally arranged around the branches in an alternate fashion. The leaflets are sensitive to light and fold inward at night. The flowers are small and bell shaped, with five petals that have whitish edges. The fruit is fleshy, have a thin and waxy skin, five lobbed, ovate to ellipsoid with attractive yellow-orange in colour.

Principle
Alkalinity of a water sample is can be determined by titrating with standard sulphuric acid. Titration to pH 8.3 or de-colourization of phenolphthalein indicator will indicate complete neutralization of OH and ½ of CO3 while to pH 4.5 or sharp change from yellow to pink of methyl orange indicator, that will indicate total alkalinity (complete neutralization of OH, CO3, HCO3)
Chemicals
1. Phenoplthalein indicator
2. Methyl orange indicator
3. Sodium carbonate, Na2CO3
4. Sodium hydroxide, NaOH
5. Sulphuric acid, H2SO4
Reagents
1. Phenolphthalein indicator: Dissolve 0.5 g in 500 ml 95% ethyl alcohol.
2. Methyl orange indicator: Dissolve 0.5 g of methyl orange in 100 ml of water and dilute to 1000 ml in deionized or distilled water (CO2 free deionized or distilled water should be use).
3. Standard Sulphuric acid, H2SO4 (0.02N): to prepare 0.1N H2SO4 take 3 ml conc. H2SO4 and dilute it in to 1000 ml of deionized or distilled water. Standardise it against standard Na2CO3.
4. Sodium carbonate, Na2CO3 (0.05 N): Take 7g standard Sodium carbonate (Na2CO3) dry at 250 oC for 4 hr and cool in desicator then weigh 5.3 g and transfer it a 1000 ml volumetric flask after that fill to mark with distilled water. Solution should be use within 1 week.

The role of plant nutrients in crop production is well established. Estimation of nutrients contents and forms in materials, which are involved in nutrient supply and dynamics is a crucial step in scientific nutrient management and crop production. There are 16 essential plant nutrients. These are carbon (C), hydrogen (H), oxygen (O), nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), iron (Fe), sulphur (S), zinc (Zn), manganese (Mn), copper (Cu), boron (B), molybdenum (Mo) and chlorine (Cl). These nutrients have to be available to the crops in quantities as required for a yield target. Any limiting or deficient nutrient (s) will limit crop growth.

Family : Arecaceae

Flowering: February - March

Fruiting April - August

Botanical Description: The plant is a large tree and grows upto 30-40m. in height. The trunk may have a circumference of 1.7 m at the base. The leaves are palmate, leathery, gray green and 1-3 m in wide with folded along at the midrib. Flowers are 90-150 cm long and branched. Fruits are ovoid, 15-20 cm in diameter with fibrous and fleshy mesocarp.

Common elements of natural water that significantly contribute to its hardness include calcium and magnesium ions of these elements leached from rocks. Water hardness reduces the utility of water for domestic use. Calcium hardness sometimes may be helpful because it provides a coating in the pipes which protects them against corrosion.
Principle
When calcium and magnesium are present in the water, EDTA reacts with calcium first. EDTA can be used to directly measure calcium when the pH is raised to a point where magnesium hydroxide is mostly precipitated and an indicator that only combines with calcium is employed. At a pH of 12 to 13, several indicators change colour to indicate that all of the Ca has been incorporated by the EDTA. The variations between an aliquot titrated at pH 10 and one titrated at pH 12 or 13 are what define the amount of magnesium.

Immediately after use, all sorts of items are thoroughly washed with water and the rubber catheters are washed with the force of tap water to remove the residual diluted semen. The glass wares are kept in 1% chromic acid solution for overnight to remove cloudiness of glass surface. Then the articles are washed with lukewarm laboratory grade detergent (ex. Labolene®) with a brush to make the articles grease free. All the items are thoroughly washed several times under distilled water and kept inverted position in a clean trough then the openings of glassware wrapped with aluminum foil / paper.

The scapula is more regularly triangular than in horse, relatively, wider at the vertebral end and narrower at the distal end. The scapular index is about T.0.6. The spine is more prominent and is placed further forward, so that the supras- pinous fossa is narrow and does not extend to the lower part of the bone. The spine is sinuous, bent backward in its middle, forward below. Its free border is somewhat thickened in its middle, but bears no distinct tuber. Instead of little more prominent, and is prolonged by a pointed production, the acromion, from which part of the deltoid muscle arises. The sub scapular fossa is shallow. The areas for the attachment of the stratus muscle are not very distinct. The nutri- ent foramen is usually in the lower third of the posterior border. The glenoid cavity is almost circular and without any distinct notch. The tuberosity is small and close to the glenoid cavity. The coracoid process is short and rounded. The cartilage resembles that of the horse. The tuberosity unites with the rest.

Various products of karanj (Pongamia glabra) are utilized for industrial, health and animal agricultural application in the Indian subcontinent. Despite a rich source of protein (CP 28 —34 %), karanj (honge) cake is found to be slightly bitter in taste and toxic owing to the presence of furanoflavonoid (Karanjin), restricting its safe inclusion in the livestock diets. Though a non polar solvent, soxhlet extraction of karanj seed cake with petroleum ether/ acetone drastically reduces karanjin content. The residual cake left after solvent extraction is proved to be safe as animal feed supplement.

Estimation of karanjin

The samples can be analysed for the toxin, karanjin as per the method of Prabhu et al. (2002).

Extraction of Karanjin from Raw and Processed Cake

Exactly 25 gm of ground and thoroughly mixed sample is weighed and transferred into the thimble and extracted for 12h using 200ml of freshly distilled methanol as solvent. Methanol extract is then cooled and filtered into preweighted round bottom flask using Whatman No.1 filter paper.The excess methanol is distilled off under vacuum. The flask containing the extract is weighed again to obtain the weight of extract by subtracting the empty flask’s weight. Sufficient amount of moisture free extract is transferred into vials for further analysis.

Family : Cucurbitaceae

Flowering & Fruiting: Most part of the year

Botanical Description: The plant is a climbing herb with angular stem and long tuberous root. The leaves are classified as palmately simple with five lobes while the shape varies from the heart to pentagon form. The size of the leaves is approximately 5-10 cm in width and length. The tendrils are long, elastic with coil-like springy character that can wrap around the host to the entire length. The flower is large and white about 4 cm in diameter and contains five long tubular petals. The fruit is oblong, narrowed apically or at both ends. The raw fruit is green in color and turns bright red when ripened. The seeds of the plant are oblong and compressed.

The cradle-to-grave dairy supply chain includes a number of operations that result in greenhouse gas (GHG) emissions related to milk production. These activities broadly include the use of agricultural inputs like fertilizers, fossil fuel for the production of animal feed and fodder, processing concentrates in cattle feed plants, enteric fermentation, manure management, and post farm gate activities like processing milk at dairy cooperative societies, milk unions, its conversion into different milk products, and their distribution through retail supply chains.

.0. Objectives

• To recognize different types of sentences and text
• To demonstrate the correct structure of sentence and text
• To demonstrate the method to transform the tense of sentence
• To idenitify the function of teaching verb tense

2.0. Basic Sentence Structure

2.1. Subject and Predicate

In the following short sentence.

There are 6 nutrients which are very important to animal body – carbohydrates, proteins, minerals, vitamins and water.
Carbohydrates: These are polyhydroxy aldehyde or ketone as in monosaccharide or polymers in oligo or polysaccharides. The question arises whether carbohydrate is a dietary essential !!! Carbohydrate may not be dietary essential, but it is definitely metabolically essentially. The organic compounds – fats, proteins are oxidized only in the presence of carbohydrates. Carbohydrates are the structural components of DNA, RNA, and some other vital organic molecules. Although less than 1% is present in the human body [because, constantly being formed and broken down in metabolism], yet without the presence of this carbohydrate, the existence of living creature is at stake. Carbohydrate in the form of glucose primarily provides energy in the body and excess amount of carbohydrate is stored as glycogen in the liver and muscle. More than 50 % of the energy value of the diet is provided by carbohydrate.
Glucose + 6 O2 6 CO2 + 6 H20 Δ E (675 k. cal).
Lipids: These are important constituents of plant and animal tissue and these can be extractable from biological materials with the usual fat solubles, for example – Ether, Chloroform, Benzene, Carbon tetrachloride, Acetone etcetra. The lipid metabolism is in dynamic state. There is constant mobilization and transportation of fatty acids from the depots. Some portion of absorbed fatty acids are degraded in the same way, while others are combined with glycerol transported back to depots. All these reactions are so balanced, that mixtures of fatty acids in the depots, blood and organs tend to remain at equilibrium
condition.

Hardness is the total soluble Ca and Mg Salts (in same cases Fe salts). It includes sulphates and chlorides along with carbonate, bicarbonate and hydroxide salts. Soft water: 0-100 mg/L, Hard water: 100 - 300 mg/L, Very hard water: > 300 mg/L CaCO3. Total hardness should be 50- 300 mg/L. Very hard water causes osmoregulatory stress to fish. Hardness below 50 mg/L reduces growth of plankton.
Reagents
1. 10.01M EDTA solution: Dissolve 3.72 g disodium salt of EDTA in distilled water and make the volume 1L by shaking.
2. Eriochrome Black T (EBT) Indicator: Add 0.2 g dry Eriochrome powder to 50 ml of triethyl amine. Add 5 ml absolute alcohol and shake it.
3. Ammonium chloride-Ammonium hydroxide buffer solution: Dissolve 17.5g ammonium chloride (NH4Cl) in 142 ml of ammonia solution (NH4OH). Add distilled water to make the volume 250 ml.
Procedure
1. Take 50ml water sample in a 250 ml conical flask.
2. Add 2 ml buffer solution and 5 drops of EBT indicator.
3. Wine-red colour will appear.
4. Titrate against 0.01M EDTA till the colour changes into bright greenish blue.